1.In Vitro Anti-influenza Virus Activities of Sulfated Polysaccharide Fractions from Gracilaria lemaneiformis
Meizhen CHEN ; Haogui XIE ; Lawei YANG ; Zaohui LIAO ; Jie YU
Virologica Sinica 2010;25(5):341-351
In this paper, in vitro anti-influenza virus activities of sulfated polysaccharide fractions from Gracilaria lemaneiformis were investigated. Cytotoxicities and antiviral activities of Gracilaria lemaneiformis polysaccharides (PGL), Gracilaria lemaneiformis polysaccharide fraction-1 (GL-1), Gracilaria lemaneiformis polysaccharide fraction-2 (GL-2) and Gracilaria lemaneiformis polysaccharide fraction-3 (GL-3) were studied by the Methyl thiazolyl tetrazolium (MTT) method, and the inhibitory effect against Human influenza virus H1-364 induced cytopathic effect (CPE) on MDCK cells were observed by the CPE method. In addition, the antiviral mechanism of PGL was explored by Plaque forming unit (PFU), MTT and CPE methods. The results showed: i) Cytotoxicities were not significantly revealed, and H1-364 induced CPE was also reduced treated with sulfated polysaccharide fractions from Gracilaria lemaneiformis; ii) Antiviral activities were associated with the mass percentage content of sulfate groups in polysaccharide fractions, which was about 13%, in polysaccharides (PGL and GL-2) both of which exhibited higher antiviral activity; iii) A potential antiviral mechanism to explain these observations is that viral adsorption and replication on host cells were inhibited by sulfated polysaccharides from Gracilaria lemaneiformis. In conclusion, Anti-influenza virus activities of sulfated polysaccharide fractions from Gracilaria lemaneiformis were revealed, and the antiviral activities were associated with content of sulfate groups in polysaccharide fractions.
2.Effect of Rehabilitation Gymnastics with Music on Postlaparoscopic Pain Syndrome
Chunlan WU ; Meizhen WEN ; Xiaoyun DENG ; Lixiu LEI ; Shi LIAO ; Xiaoqing ZHENG ; Cuilan MA
Chinese Journal of Rehabilitation Theory and Practice 2009;15(6):591-592
Objective To observe the effect of rehabilitation gymnastics with music on non-incisional pain of gynecological patients after laparoscopic operation.Methods 192 gynecological patients undergone laparoscopic operation were divided into the music group (66 cases, adopted rehabilitation gymnastics matching music after operation), non-music group (64 cases, adopted rehabilitation gymnastics) and routine group (62 cases, adopted routine nursing without rehabilitation gymnastics or music).Results The patients of the music group got pain eased more obviously than those of the non-music group and rule group ( P<0.05).Conclusion The rehabilitation gymnastics with music can relieve non-incisional pain after gynecological laparoscopic operation, and improve anus exhausting.
3.Analysis on the septum correction and plasma radiofrequency ablation combination therapy for nasal septum deviation.
Weixin HUANG ; Jiannan HUANG ; Meizhen LIAO ; Jijun GUO ; Yuzhen YANG
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2011;25(8):352-353
OBJECTIVE:
To discuss a effective method of nasal septum deviation.
METHOD:
One hundred and sixteen subjects with nasal septum deviation were divided into treatment group (69 subjects) and control group (47 subjects) randomly. The combination therapy of correction of deviated nasal septum and plasma radiofrequency ablation were used in the treatment group. The combination therapy of deviated nasal septum and partial inferior turbinectomy were used in the control group. The data were analyzed by statistical method.
RESULT:
The effective rate of physical signs and symptoms of the treatment group was 100.0%, while the control group was 85.1%. There was significant difference of two effective rates (P < 0.05). Furthermore, hyperventilation and nasal adhesion were not happened in treatment group 6 months after treatments.
CONCLUSION
The correction of deviated nasal septum and plasma radiofrequency ablation combination therapy had the satisfied and safety treatment effect, which was and plasma radiofrequency ablation combination therapy had the satisfied and safety treatment effect, which was easy for observation as well as the minimal tissue damage. The combination therapy method was according with the principle of functional minimally invasive surgery.
Adult
;
Catheter Ablation
;
Combined Modality Therapy
;
Female
;
Humans
;
Male
;
Middle Aged
;
Nasal Septum
;
abnormalities
;
surgery
;
Reconstructive Surgical Procedures
;
Young Adult
4. Survival analysis of AIDS patients after antiretroviral treatment in Shandong province during 2003 to 2017
Xiaoyan ZHU ; Guoyong WANG ; Na ZHANG ; Meizhen LIAO ; Pengxiang HUANG ; Xingguang YANG ; Tao HUANG ; Xiaorun TAO ; Dianmin KANG
Chinese Journal of Experimental and Clinical Virology 2019;33(2):187-192
Objective:
To understand the survival status of AIDS patients after initiation of antiretroviral treatment (ART) in Shandong province during 2003 to 2017, and to determine the factors associated with survival status.
Methods:
A retrospective cohort study was conducted among AIDS patients initially received antiretroviral therapy from 2003 to 2017. The epidemiological characteristics of the subjects were described, and life tables were used to estimate the survival rates, the influencing factors were analyzed by Cox regression model.
Results:
A total of 9 813 cases were enrolled in this study, of the subjects, the median age was 34.9±11.0 years. The median of baseline CD4+ T lymphocyte count was 300.0(
5.Studies on behavior change and related factors among female sex workers in Qingdao city of Shandong province, between 2006 and 2015
Meizhen LIAO ; Xiaoyan ZHU ; Pengxiang HUANG ; Zhenxia JIANG ; Xijiang ZHANG ; Ning ZHANG ; Guoyong WANG ; Yuesheng QIAN ; Xiaorun TAO ; Dianmin KANG
Chinese Journal of Epidemiology 2017;38(12):1660-1664
Objective This study aimed to analyze the behavior change and related factors regarding HIV/STD epidemics among female sex workers (FSWs) in Qingdao city.Methods According to the requirements set by the "National HIV/AIDS sentinel surveillance program",information on demographics,sexual and drug use behaviors,and HIV-related services among female sex workers (FSWs) was collected from ten consecutive annual cross-sectional surveys from 2006 to 2015.Blood samples were drawn for serological tests on both HIV and syphilis antibodies.Results Data from the sampled FSWs over the ten years,a higher proportion of participants who were aged 30 or more,married or cohabited and on-call FSW were followed.The prevalence of syphilis increased significantly from 1.0% (4/420) in 2006 to 13.3% (53/400) in 2015 (trendx2=54.22,P<0.001).Rates on illicit drug use were ranging from 12.0% (48/400) and 55.5% (222/400) while the rate on consistent condom use with clients in the last month showed decreasing,with trendx2=170.62,P<0.001.The proportion of HIV-related knowledge score ≥ 6 (trend x2 =152.96,P< 0.001),or ever been tested for HIV (trendx2=114.87,P<0.001) were both significantly increased over the last ten years.Between 2009 and 2015,results from the annual stratified analysis showed that the FSWs who used drugs were more likely than the FSWs who were non-drug users less consistently using condoms with clients in last month and being syphilis positive (P<0.05).On-call FSWs were more likely to be syphilis positive (P<0.05) than the non on-call FSWs.Conclusions The prevalence of syphilis among FSWs in Qingdao city had been rising over the last ten years,with synthetic drug abuse as an important risk factor.Better targeted surveillance and intervention efforts among those drug-using FSWs seemed important to reduce the epidemics.
6. Behavior and influencing factors of HIV infection among men who have sex with men only and men who have sex with both men and women in Shandong province
Meizhen LIAO ; Dongying LIU ; Xiaoyan ZHU ; Pengxiang HUANG ; Guoyong WANG ; Yuesheng QIAN ; Xiaorun TAO ; Dianmin KANG
Chinese Journal of Epidemiology 2019;40(8):941-946
Objective:
This study aimed to examine the demographic characteristics, HIV related knowledge and behavior, correlates of bisexual behavior and status of HIV infection among men who have sex with men only (MSMO) and men who have sex with both men and women (MSMW) in Shandong province.
Methods:
According to the requirements from "National HIV/AIDS sentinel surveillance program" , a cross-sectional survey was conducted to collect information on demographics, sexual and drug use behaviors, and HIV-related services among MSM in nine sentinel surveillance sites from April to July in 2018. Blood samples were drawn for serological tests on both HIV and syphilis antibodies.
Results:
A total of 3 474 participants were included in this study. Related information on these participants would include: average age as (31.66±9.01) years; 35.06% (1 218) married or cohabiting with a woman, 50.52% (1 755) had college or higher education, 80.11% (2 783) self-identified as gays and 14.22% (494) self-identified as bisexual men,16.87% (586) ever having sex with woman in the past 6 months, 10.51% (365) ever using drugs. HIV and syphilis prevalence rates were 2.99% (104/3 474) and 2.76%(96/3 474). Through multivariable logistic models, MSMW were more likely to be ≥35 years of age, local residents, self-identified as heterosexual/bisexual/uncertain, ever having commercial sex with man but less likely to consistently use condoms in the past 6 months, less using internet/dating software to find male sex partners and less using drugs. There was no significant differences noticed in the following areas: number of sexual partners in the last week, condom use in the last six months with commercial sex partners, with HIV or syphilis infection and self-reported history of STD in the past year between MSMO and MSMW (