High efficient dyes decolorizing bacterium, strain S12~T, was isolated from activated-sludge of textile-printing wastewater treatment plant. The strain was identified as a novel specie of the genus Shewanella, for which the name Shewanella decolorationis sp. nov. is proposed. It's decolorizing rate reached 96% in 4h, when 50mg/L of azo dye was used. The organism exhibited a remarkable color removal capability, even at azo dye's concentration of 2,000mg/L. A clear decolorizing zone around each colonies appeared after four days grown on LB plate containing 500mg/L azo dye. The changes of UV-visible spectra of azo dye solution indicate that the color removal was largely attributed to biodegradation. The decolorizing enzymes of strain S12~T were constitute type and not secreted to the culture medium.

Biological process is the important approach to treat the dye pollutants.This paper gives a systematic introduction to the three types of important dyes,azo dyes,triphenylmethane dyes and anthraquinone dyes,including azo reduction and mechanism under anaerobic and aerobic conditions,advances of triphenylmethane dyes and anthraquinone dyes biodegradation.

Dissimilatory Fe(Ⅲ)reduction is the important process in Biogeochemical cycle.This paper gives a systematic introduction to the types of dissimilatory Fe(Ⅲ)reduction,mechanism of insoluble Fe(Ⅲ)oxidizes reduction and the advances of molecular biology in- volved in Fe(Ⅲ)reduction.The status of the applications of dissimilatory Fe(Ⅲ)reduction in environmental contaminant treatment were also discussed.

Objective To investigate the effect of forced-air warming system on the cellular immune function during radical esophagus cancer resection. Methods Thirty-six ASA Ⅰ or Ⅱ patients of both sexes, aged ≤ 64 yr, with body mass index ＜ 30 kg/m2 , scheduled for elective radical esophagus cancer resection, were randomized to 2 groups ( n = 18 each): normal temperature care group (group C) and forced-air wanning group (group T) . Anesthesia was induced with midazolam, sufentanil, propofol and vecuronium. The patients were tracheal intubated and mechanically ventilated. The patients were not warmed intraoperatively in group C. In group T, the patients were prewarmed for 20 min at 43℃, using forced-air warming system before induction and then kept warm until the end of operation. The nasopharyngeal temperature was measured at 0, 30, 60, 120 and 180 min after anesthesia induction and at the end of operation (T1-6 ) to reflect the body temperature. Venous blood samples were taken at T1,6 for analysis of T-lymphocyte subsets (CD3+ , CD4+ , CD8+ , CD4+ /CD8+ ) and NK cells (by flow cytometry) and determination of the plasma concentrations of noradrenaline and adrenaline (by ELISA) . Results Compared with T1 , the body temperature was significantly decreased at T2-6 in group C, and the percentage of CD4+ cells and CD4+ /CD8+ ratio were significantly decreased and the percentage of CD8+ and plasma concentrations of noradrenaline and adrenaline increased at T6 in both groups ( P ＜ 0.05). Compared with group C, the body temperature was significantly increased at T2-6, plasma concentrations of noradrenaline and adrenaline were significantly increased at T, , while the change rate of concentrations was significantly decreased in group T ( P ＜ 0.05) . ConclusionThe efficiency of forced-air warming system in maintaining perioperative normothermia is good and it reduces the stress response, but it exerts no influence on the cellular immune function in patients undergoing radical esophagus cancer resection.

Objective To study the species and drug resistance of Mycobacterium isolated from patients with spu-tum smear positive for acid-fast bacillus in Wuj iang city,and provide reference for the prevention and control of tu-berculosis. Methods Sputum specimens with positive smear were cultured,isolated bacteria were identified and performed drug susceptibility testing,drug resistance among different species of strains and between patients with initial and repeated treatment were compared.Results A total of 1 03 Mycobacterium isolates were included in the study,13 of which were nontuberculous Mycobacterium,drug resistance rate was 100.00% ,multidrug resistance (MDR)rate was 84.62% ;90 isolates were Mycobacteriumtuberculosis,81(90.00% )of which were Mycobacteri-umhominis. Drug resistance rate of Mycobacteriumtuberculosiswas 35.56% ,MDR rate was 14.44% . Of 70 ini-tially treated tuberculosis patients with positive sputum smear,14(20.00% )were resistant to drugs,MDR rate was 4.28% (3/70);Of 20 repeatedly treated tuberculosis patients with positive sputum smear,18(90.00% )were resist-ant to drugs,MDR rate was 50.00% (10/20).Conclusion Mycobacteriumtuberculosisis the major isolated strain from patients with positive sputum smear. Drug resistance and MDR rates of nontuberculous Mycobacterium are very high. Drug resistance and MDR rates of Mycobacteriumtuberculosisin repeatedly treated patients are higher than initially treated patients.

Objective To investigate the effect of L-N6-(1-iminoethyl) Lysine(L-NIL) on ischemia-reperfusion (I/R) -induced lung injury in a rat model of lung transplantation. Methods Pathogen free male SD rats weighing 250-350g were used as donor and recipient rats in this study. The animals were randomly divided into 3groups (n = 6 each): sham operation group (group S); lung tratsplantation group (group L) and lung transplantation + L-NIL (selective iNOS inhibitor) group (group L-NIL). In group L and L-NIL orthotopic left lung allograft transplantation was performed. In group L-NIL 3 mg/kg was injected iv at the beginning of reperfusion. The donor lungs were removed from live donor rats and placed in Euro-collins solution at 4 ℃. The lung transplantation was performed under microscope and non-suture cuff technique was used. The implanted donor lungs were ventilated and reperfused. 0.5% Evans blue 0.2 ml was injected iv during reperfusion. The donor lungs were removed after being implanted, ventilated and reperfused for 2 h for microscopic examination and determination of iNOS, endothelial NOS (eNOS) and myeloperoxidase (MPO) activity and malondialdehyde (MDA) and Evans blue content in the lung tissue and W/D lung weight ratio. Results Lung transplantation significantly inceased W/D ratio, iNOS and MPO activity, and Evans blue and MDA content in the lung tissue and decreased eNOS activity in group L as compared with group S. L-NIL iv significantly attenuated the increase in the variables mentioned above and ameliorated capillary congestion and inflammatory cell infiltration in the lung. Conclusion Intravenous L-NIL administered at the beginning of reperfusion can reduce I/R injury to the transplanted donor lungs.

Objective:To investigate the relationship between PaCO 2 and PetCO 2 while the respiration pattern changed from spontaneous to mechanical ventilation during artificial valve replacement. Methods:The changes of PaCO 2,PetCO 2,CO,MAP,PAP,CVP,PAWP,SVR,PVR and V D/V T,alv from spontaneous respiration to mechanical ventilation were observed pre and post general anesthesia in 22 patients undergoing artificial valve replacement. Results:Compared with spontaneous respiration before general anesthesia induction, the differences between PaCO 2 and PetCO 2,CVP,V D/V T,alv ratio were significantly increased( P

Objective To explore the method of anesthesia and intra-operative management for robotic thoracic surgery. Methods Twelve patients who underwent robotic thoracic surgery using the Da Vinci surgical system were anesthetized with general anesthesia combined with T4-8 paravertebral block. After induction of anesthesia, a double-lumen endotracheal tube was positioned by bronchofibroscope to allow onelung ventilation during intra-operative procedure. Hemodynamics and respiratory function were routinely monitored and arterial blood gas (ABG) were tested during operation. Results All patients could tolerate the anesthesia for robotic thoracic surgery and there was no hospital mortality. The arterial carbon dioxide tension (PaCO2) and arterial oxygen tension (PaO2) after induction were (35.2 ± 3.6) mm Hg( 1 mm Hg =0.133 kPa) and (213.3 ± 57.5) mm Hg respectively; PaCO2 and PaO2 30 min after one -lung ventilation were (37.9 ± 4.8) mm Hg and ( 125.3 ± 36.5) mm Hg respectively. When the one-lung ventilation started about 58% (7/12) of the patient developed temporarily low SpO2 (over 0.90) and recovered to 0.95 soon when using 3 - 5 cm H2O( 1 cm H2O = 0.098 kPa) positive end expiratory pressure (PEEP). The anesthesia time was ( 291.5 ± 99.4) min, the time for one-lung ventilation was (206.3 ± 93.4) min, the volume of blood loses in operation was ( 171.7 ± 110.3 ) ml and the tracheal catheter extration time was ( 16.3 ± 4.5 ) min, all the patients left ICU on the second day after surgery. Conclusions The anesthesia for robotic thoracic surgery with Da Vinci surgical system is multiplicity, the hemodynamics and respiratory function can be instable, it is a new challenge for the technology and management of anesthesia. Good one-lung ventilation is important for this surgery, ventilation parameter need to be adjusted when hypoxia occurred and PEEP could be used to the ventilated lung. General anesthesia combined with paravertebral block will be a good option for postoperative pain control and minimal hemodynamics disturb ance.

Pulmonary function changes during and after cardiopulmonary bypass were studied using single breath test for CO_2 (SBT-CO_2) in 16 adult patients undergoing cardiac surgery. The results showed that P_(A-a)CO_2 and P_(A-a)O_2 increased significantly after bypass though PaCO_2 kept in normal range by adjusting ventilation volume. The CO_2 production increased as time passed after bypass, resulting in the increase of required minute volume and the rise of airway pressure. Compliance showed a tendency to decrease, while physologic dead space and alveolar dead space increased significantly. It is concluded that the causes of pulmonary dysfunction occurring in early stage of post-bypass are mainly due to the V/Q mismatch induced by low perfusion of the lung. To improve the pulmonary function at the early stage of post-bypass, the circulatory function should be improved accordingly.

Objective: To evaluate the effect of propofol on coronary circulation of acute myocardial ischemia-reperfusion inury. Metbod: 18 hybrid dogs (11-14kg) were divided randomly into three groups:NS group (normal saline, 2ml?kg~(-1)?h~(-1)), HP group (high-dose propofol, 11.2mg?kg~(-1)?h~(-1)), LP group (low-dose propofol, 5.6mg?kg~(-1) ?b~(-1)). These dogs were subjected to 90 min left anterior descending coronary artery (LAD) occlusions followed by 200 min of reperfusions. Before administration, 60 min after administration, 60min, 120min, 180min following reperfusion,coronary circulation was assessed by measurement of coronary perfusion pressure (CPP) and blood flow of LAD. Result: The values of CPP in HP and LP groups 60 minafter administration were significantly lower than those in NS group before LAD occlusion (P