Objective To study the polymorphisms of the large mutiplefunctional protease (LMP2) genes in women with severe pre-eclampsia (S-PE) and the sharing rate of LMP 2 in these women and their partners and couples. Methods Totally, 102 S-PE women and their husbands were enrolled in the study group, and 200 normal pregnant women and their husbands as control. The types of LMP2 alleles were assessed by polymerase chain reaction-restriction fragment lengthpolymorphism method (PCR-RFLP) after PCR. Results The frequency of LMP2R/H in S-PE group was significantly higher than that of the control (53.9% vs 35.%, P

Objective To study the association between paternal CD3, CD4 and CD8 antigenecity to their pregnant spouses and the development of pregnancy induced hypertension (PIH). Methods Maternal serum from 15 women with PIH in the third trimester and 82 in normal pregnancies (16 in the first, 32 in the second and 34 in the third trimester) were incubated with paternal T lymphocytes. Monoclonal CD3, CD4 and CD8 fluorescent conjugated antibodies were then added and the percentage of paternal T cell differentiation antigen CD3, CD4 and CD8 were measured by flow cytometry. Results During normal pregnancy, the levels of maternal serum blocking antibodies on paternal CD3, CD4 and CD8 were (4.14?1.02, 2.02 ?0.24, 2.37?1.05)% in first trimester, (-0.29?0.13, 1.03?0.27, 0.65?0.23)% in the second trimester and (-1.33?1.47,0.15?0.01, -1.04? 0.37)% in the third trimester. There were significant difference between them( P

Objective To investigate the risky sexual behaviors,associated factors and characteristics of sexual network among clients receiving methadone maintenance treatment (MMT) at the clinics in Taizhou prefecture of Zhejiang province.Methods A cross-sectional survey was conducted.Data was collected through questionnaire and from the national unified MMT system.Urine was collected to test heroin,methamphetamine,3,4-methylenedioxymethamphetamine (MDMA) and ketamine.Blood was collected to test infections on HIV,hepatitis C vims(HCV) and syphilis.Results Totally,362 clients were recruited.Most of the clients were male (88.7％),31--40 year-olds (54.5％),married (61.6％) and having received junior high school education (56.6％).85.1％ of them received urine test with 82(26.6％) positive for heroin,21 (6.8％) positive for methamphetamine,19 (6.2％) positive for MDMA and none for ketamine.77.1％ of them received blood test,and prevalence rates for HIV,HCV and syphilis were 1.1％,36.7％ and 3.6％.19.5％ of the clients who had sex in the past 6 months.Factors as having multiple sexual partners and positive for club drugs were under higher risk.Sexual networking seemed to be loose,linear and acyclic among this population but overlapping with the drug-using network.Conclusion Effective and targeted interventions should be taken among the MMT clients since continuing drug use and HIV/STD related sexual behavior were found common in them,suggesting there was a risk of HIV/STD transmission in this ppulation.

Objective To examine the prevalence and correlates of hepatitis C virus (HCV) co-infection as well as plasma HCV viral load among newly diagnosed HIV-infected adults during 2009-2012 in Taizhou prefecture of Zhejiang province,China.Methods Five hundred and seventy-two adults who were newly diagnosed as HIV-infection were screened for anti-HCV immunoglobulin G (IgG).Plasma HCV RNA was quantified if positive for HCV IgG.Results Forty-two (7.3％) out of the 572 HIV-infected adults were tested positive for HCV IgG.HCV infection was more likely to occur among participants who were infected with HIV through blood transmission (OR=49.46,95％CI:13.71-178.48).Otherwise,HCV infection was less likely to occur among participants who were under 46-86 years of age (OR=0.12,95％CI:0.02-0.58),those with mode of homosexual transmission of HIV (OR=0.11,95％ C I:0.01-0.86),with education of high school or above (OR=0.13,95％ CI:0.02-0.78).Thirty-three (78.6％) of the total 42 HIV-HCV patients with coinfection had detectable plasma HCV RNA,whereas 9 (21.4％) had undetectable plasma HCV RNA (i.e.,lower than 5.0 × 102 IU/ml) or known as relative HCV viral suppression.No significant associations were noticed on factors as:HCV viral suppression and demographics,HIV transmission route and CD4 + T-cell counts according to univariate regression analyses.Conclusion Prevalence of HCV co-infection was relatively high among newly diagnosed HIV-infected adults in this study area.Majority of the HIV-HCV coinfected patients had detectable plasma HCV RNA,of which the long term impact on disease progression deserves called for further research.