Objective To investigate the effect of propofol on the function of gap junction (GJ) in HeLa cells transfected with Cx32/Cx26 plasmid. Methods Cervical cancer HeLa cells transfected with Cx32/Cx26 was given as present by professor Andrew L. Harris from New Jersey Dental Medical School, department of pharmacology and physiology. The transfected cells were selected by G-418. The effective GJ channels were identified by "Parachute Assay". The cells were randomly divided into 6 groups: Ⅰ control group (group C); Ⅱ fat emulsion group was exposed to fat emulsion 10 μg/ml (group E); Ⅲ 18-α-GA group was exposed 18-α-GA (gap junction blocker) 1.0 μg/ml (18-α-GA); Ⅳ, Ⅴ, Ⅵ propofol groups were exposed to propofol 1.3, 2.2 and 3.2μg/ml respectively (group P1, P2, P3). The transfected HeLa cells were incubated at 37 ℃ for 4 h. Gap junction function was assessed using fluorescent indicators Calcine-AM which emits green fluorescence and CM-Dil which emits red fluorescence. The small molecular Calcine-AM can pass through gap junction and enters HeLa cells while the large molecular CM-Dil cannot pass through gap junction and stays in the loading cells. Fluorescent indicator transmissibility and inhibition rate were calculated. Results The fluorescent indicator transmissibility was significantly lower and inhibition rate higher in group 18-α-GA, P1, P2 and P3 than in control group. There was nosignificant difference in the fluorescent indicator transmissibility and inhibition rate between group C and E. The inhibition of GJ function by propofol was dose-dependent. Conclusion Propofol can inhibit the function of GJ in HeLa cells transfected by Cx32/Cx26 in a dose-dependent manner.

Objective: To retrospectively analyze the diagnosis time, pathogen distribution, and drug resistance of fungal bloodstream infection in severe burn patients. Methods: Blood samples were collected from 55 severe burn patients with fungal bloodstream infection (including 46 males and 9 females, aged 42 (1, 78) years) admitted to the intensive care unit of the Institute of Burn Research of the First Affiliated Hospital of Army Medical University (the Third Military Medical University) from July 2011 to May 2019 for retrospective analysis. Microbial monitoring system was used to cultivate pathogens, API yeast identification kit and Candida chromogenic medium were used to identify pathogens, and Kirby-Bauer paper disk diffusion method was used to detect drug resistance of fungi to fluconazole, amphotericin B, itraconazole, ketoconazole, and voriconazole. The positive rate of blood fungal culture, mortality rate, distribution of local fungal proliferation sites, the diagnosis time distribution of fungal bloodstream infection, the distribution of fungal species, resistance to commonly-used antifungal drugs, and the use of antibiotics were assessed. The WHONET 5.6 software was applied to analyze the distribution and drug resistance of fungi. Results: (1) Totally 4 839 blood samples were collected during the 9 years, and 122 strains of fungi were isolated, with positive rate of 2.52%. The mortality rate was 14.55% (8 patients) in 55 patients. Catheter fungal proliferation ranked the first among 30 cases of local fungal proliferation. (2) The diagnosis time of fungal bloodstream infection mainly distributed in ≤1 week of hospitalization [32.73% (18/55)]. (3) Among the 55 strains of fungi detected, the detection rate of Candida parapsilosis ranked the first (21.82%, 12 strains), Candida glabrata was the second (18.18%, 10 strains), and Candida tropicalis was tied with Candida albicans in the third place (14.55%, 8 strains). All the detected fungi were sensitive to amphotericin B, and the resistance rates to voriconazole, fluconazole, itraconazole, and ketoconazole were between 4.5% and 9.1%. (4) Droad-spectrum antibiotics were used in all the 55 patients, ≥3 kinds of antibiotics were used in 44 patients, and 37 patients used antibacterial drugs ≥7 days. Conclusions The diagnosis time of fungal bloodstream infection in the 55 severe burn patients was mainly within 1 week of hospitalization. Candida parapsilosis is the most commonly detected fungal species. Catheter fungal proliferation occurs most commonly among the 30 patients with local fungal proliferation. All the detected fungi were sensitive to amphotericin B, with low drug resistance to voriconazole, fluconazole, itraconazole, and ketoconazole. Broad-spectrum antibiotics were overused in the severe burn patients with fungal bloodstream infection.

Objective: To investigate the clinical characteristics of burn patients infected with Stenotrophomonas maltophilia (SM) and antibiotic resistance of the strains. Methods: Clinical data of burn patients detected with SM, admitted to our unit from July 2011 to July 2017 were retrospectively analyzed. API 20NE bacteria identification panel or fully automated microbial identification instrument was used to identify pathogen. Minimal inhibitory concentration method was used in drug sensitivity test of levofloxacin, compound sulfamethoxazole, minocycline, and cefoperazone/sulbactam. Annual detection of SM, clinical characteristics and prognosis of patients infected with SM, sample source and detection time of SM, detection of the pathogens and antibiotics application of patients before their detection of SM, and drug resistance of SM to the above four antibiotics were analyzed. The results of drug sensitivity test were analyzed by software WHONET 5.5. Results: (1) There were totally 119 patients detected with SM, with 11, 12, 21, 22, 28, 13, and 12 cases from 2011 to 2017, respectively. (2) Among patients infected with SM, there were 86 (72.3%) males and 33 (27.7%) females. Patients aged more than or equal to 65 years accounted for 11.8% (14/119). Patients aged more than or equal to 18 years and less than 65 years accounted for 76.5% (91/119). Patients aged less than 18 years accounted for 11.8% (14/119). Patients with scald were the most common (totally 72 cases, accounted for 60.5%), and patients with total burn area less than or equal to 10% total body surface area were the most common (totally 35 cases, accounted for 29.4%), too. The proportion of patients with history of basic disease was 16.8% (20/119), with tracheotomy of 46.2% (55/119), with deep vein catheterization of 47.9% (57/119), with history of staying in intensive care unit (ICU) of 61.3% (73/119). Seventy-five (63.0%) patients were cured. Twenty-four (20.2%) patients were improved. Fourteen (11.8%) patients gave up treatment. Six (5.0%) patients died. (3) SM detected from wounds exudate of patients occupied the highest proportion (58.0%, 69/119), which was followed by samples of sputum (17.6%, 21/119), blood (14.3%, 17/119), wound tissue (4.2%, 5/119), catheter (4.2%, 5/119), and urine (1.7%, 2/119). The detection time of SM was 10 hours to 71 days post admission, with the average time of 12.7 days. (4) The proportion of patients detected with pathogens before detection of SM was 66.4% (79/119), and Acinetobacter baumannii and Staphylococcus aureus occupied high proportion among the strains. (5) The proportion of patients using antibiotics before detection of SM was 91.6% (109/119), and 44.0% (48/109) patients used 3 kinds of antibiotics or more. The antibiotics were applied for 271 times. The most frequently used antibiotics were glycopeptides antibiotics (63 times), followed by carbapenems antibiotics (61 times). (6) The total sensitivity rates of SM to levofloxacin and minocycline in 7 years were high (91.6% and 99.4%, respectively). The total sensitivity rate of SM to cefoperazone/sulbactam was low (52.5%). The total sensitivity rate of SM to compound sulfamethoxazole was high (77.6%), and the annual sensitivity rate was higher than 90.0% in recent 3 years. Conclusions Burn patients infecting SM have high rates of tracheotomy and deep vein catheterization, and most of them stay in ICU and use broad-spectrum antibiotics. SM has high sensitivity to levofloxacin, minocycline, and compound sulfamethoxazole.

Objective: To analyze the distribution and drug resistance of pathogens isolated from patients with catheter-related bloodstream infection (CRBSI) in burn intensive care unit (BICU). Methods: From January 2011 to December 2018, among 2 264 patients who were peripherally inserted central venous catheter at the BICU of the First Affiliated Hospital of Army Medical University (the third Military Medical University), hereinafter referred to as the author′s unit, 159 patients were diagnosed CRBSI, including 131 males and 28 females, aged 43 (1, 79) years. The pathogens primarily isolated from peripheral venous blood and central venous catheter blood/anterior central venous catheter specimen of patients with CRBSI were retrospectively analyzed. API bacteria identification kits and automatic microorganism identification instrument were used to identify pathogens. Broth micro-dilution method or Kirby-Bauer paper disk diffusion method was used to detect the drug resistance of the pathogens to 5 antifungal drugs including fluconazole and itraconazole, etc., and 37 antibacterial drugs including tigecycline and imipenem, etc. Modified Hodge test was used to further identify imipenem- and meropenem-resistant Klebsiella pneumonia. D test was used to detect erythromycin-induced clindamycin resistant Staphylococcus aureus. The WHONET 5.6 software was applied to analyze the annual incidence of CRBSI, mortality of patients with CRBSI, incidence of CRBSI cases, distribution of infection site, and duration of catheterization, detection of Gram-negative and Gram-positive bacteria, fungi, methicillin-resistant Staphylococcus aureus (MRSA), and methicillin-sensitive Staphylococcus aureus (MSSA), and drug resistance of fungi and major Gram-negative and Gram-positive bacteria to the commonly used antibiotics in clinic. Results: (1) The incidence of CRBSI was 7.0% (159/2 264) during the eight years, which was slightly higher in 2014 and 2017 with 13.6% (30/221) and 11.1% (24/217) respectively. The mortality rate of patients with CRBSI was 7.5% (12/159). (2) The incidence of CRBSI cases was 14.9% (338/2 264); the main infection site was femoral vein, totally 271 cases (80.2%), and the duration of catheterization of this site was 9 (2, 25) d. (3) During the eight years, totally 543 strains of pathogens were isolated, including 353 (65.0%) strains of Gram-negative bacteria, 140 (25.8%) strains of Gram-positive bacteria, and 50 (9.2%) strains of fungi. The top three isolated pathogens with isolation rate from high to low were Acinetobacter baumannii, Staphylococcus aureus, and Pseudomonas aeruginosa, accounting for 23.2% (126/543), 17.1% (93/543), and 15.7% (85/543), respectively. Fungi were mainly Candida parapsilosis. Among the Staphylococcus aureus, the detection rate of MRSA was 98.9% (92/93), and that of MSSA was 1.1% (1/93). (4) Except for the low drug resistance rates to polymyxin B, minocycline, and tigecycline, the drug resistance rates of Acinetobacter baumannii to the other antibiotics were considerably high (80.1%-100.0%). Pseudomonas aeruginosa was not resistant to polymyxin B but highly resistant to netilmicin (88.7%) and piperacillin (92.6%), with resistance rates to the other antibiotics from 34.5% to 62.7%. Klebsiella pneumoniae was not resistant to tigecycline and lowly resistant to imipenem and meropenem (28.9%, 9 imipenem- and meropenem-resistant strains were further confirmed by modified Hodge test), with resistance rates to the other antibiotics from 40.9% to 95.2%. The resistance rates of MRSA to most antibiotics were higher than those of MSSA. MRSA was not resistant to linezolid, vancomycin, teicoplanin, sulfamethoxazole, or tigecycline. The resistance rates of MRSA to clindamycin and erythromycin were 7.9% and 62.0%, respectively, and those to the other antibiotics were higher than 91.5%. Except for the complete resistance to penicillin G and tetracycline, MSSA was not resistant to the other antibiotics. Thirty-three strains of Staphylococcus aureus showed resistance to erythromycin-induced clindamycin. Fungi was not resistant to amphotericin B, with drug resistance rates to voriconazole, itraconazole, ketoconazole, and fluconazole from 4.2% to 6.2%. Conclusions The incidence of CRBSI and mortality of patients with CRBSI are high in BICU of the author′s unit, and the main infection site is femoral vein. There are various types of pathogens in patients with CRBSI, and most of them are Gram-negative. The top three isolated pathogens are Acinetobacter baumannii, Staphylococcus aureus, and Pseudomonas aeruginosa, accompanying with grim drug resistance phenomenon.

Purpose: The systemic inflammation response index (SIRI) has been reported to have prognostic ability in various solid tumors but has not been studied in gallbladder cancer (GBC). We aimed to determine its prognostic value in GBC. Materials and Methods: From 2003 to 2017, patients with confirmed GBC were recruited. To determine the SIRI’s optimal cutoff value, a time-dependent receiver operating characteristic curve was applied. Univariate and multivariate Cox analyses were performed for the recognition of significant factors. Then the cohort was randomly divided into the training and the validation set. A nomogram was constructed using the SIRI and other selected indicators in the training set, and compared with the TNM staging system. C-index, calibration plots, and decision curve analysis were performed to assess the nomogram’s clinical utility. Results: One hundred twenty-four patients were included. The SIRI’s optimal cutoff value divided patients into high (≥ 0.89) and low SIRI (< 0.89) groups. Kaplan-Meier curves according to SIRI levels were significantly different (p < 0.001). The high SIRI group tended to stay longer in hospital and lost more blood during surgery. SIRI, body mass index, weight loss, carbohydrate antigen 19-9, radical surgery, and TNM stage were combined to generate a nomogram (C-index, 0.821 in the training cohort, 0.828 in the validation cohort) that was significantly superior to the TNM staging system both in the training (C-index, 0.655) and validation cohort (C-index, 0.649). Conclusion The SIRI is an independent predictor of prognosis in GBC. A nomogram based on the SIRI may help physicians to precisely stratify patients and implement individualized treatment.

Objectives:To investigate the prevalence,clinical characteristics and risk factors of coronavirus disease-2019(COVID-19)in patients with pulmonary hypertension(PH). Methods:A questionnaire survey was conducted from December 30,2022 to January 6,2023 through the WeChat official account of the PH Patients Mutual Aid Organization.PH patients aged≥18 years from 26 province(municipality/autonomous region)were recruited to fill in the electronic survey questionnaire. Results:A total of 293 valid questionnaires were collected from PH patients.The mean age of patients was(40.6±12.7)years,and 226 patients(77.1%)of them were female.The vaccination rate was 59.7%(175/293),117 patients(39.9%)received three or more doses of vaccine,145 patients(49.5%)received inactivated vaccine.242 patients(82.6%)had COVID-19.The most common symptoms during infection were fever(85.5%),cough(77.7%),and fatigue(66.5%).10.7%of the patients had severe or critical COVID-19.Age(OR =1.057,95%CI:1.027-1.087,P<0.001)and comorbid pulmonary disease(OR=3.341,95%CI:1.215-9.184,P=0.019)were associated with severe or critical COVID-19.After adjusting for confounding factors,age was an independent risk factor for severe or critical COVID-19(OR=1.049,95%CI:1.019-1.080,P=0.001).Severe or critical COVID-19 was an independent risk factor for worsening heart failure in PH patients during COVID-19 pandemic(OR=10.522,95%CI:4.311-25.682,P<0.001). Conclusions:The immunization coverage of PH patients is insufficient.PH patients have a higher risk of developing severe or critical COVID-19 than general population.Ageing is an independent risk factor for severe or critical COVID-19,and the risk of worsening heart failure in PH patients with severe or critical COVID-19 is significantly increased during COVID-19 pandemic.

Objective To analyze the distribution and drug resistance of pathogen isolated from severely burned patients with bloodstream infection,so as to provide reference for the clinical treatment of these patients.Methods Blood samples of 162 severely burned patients (including 120 patients with extremely severe burn) with bloodstream infection admitted into our burn ICU from January 2011 to December 2014 were collected.Pathogens were cultured by fully automatic blood culture system,and API bacteria identification panels were used to identify pathogen.Kirby-Bauer paper disk diffusion method was used to detect the drug resistance of major Gram-negative and-positive bacteria to 37 antibiotics including ampicillin,piperacillin and teicoplanin,etc.(resistance to vancomycin was detected by E test),and drug resistance of fungi to 5 antibiotics including voriconazole and amphotericin B,etc.Modified Hodge test was used to further identify imipenem and meropenem resistant Klebsiella pneumonia.D test was used to detect erythromycin-induced clindamycin resistant Staphylococcus aureus.The pathogen distribution and drug resistance rate were analyzed by WHONET 5.5.Mortality rate and infected pathogens of patients with extremely severe burn and patients with non-extremely severe burn were recorded.Data were processed with Wilcoxon rank sum test.Results (1) Totally 1 658 blood samples were collected during the four years,and 339 (20.4％) strains of pathogens were isolated.The isolation rate of Gram-negative bacteria,Gram-positive bacteria,and fungi were 68.4％ (232/339),24.5％ (83/339),and 7.1％ (24/339),respectively.The top three pathogens with isolation rate from high to low were Acinetobacter baumannii,Staphylococcus aureus,and Pseudomonas aeruginosa in turn.(2) Except for the low drug resistance rate to polymyxin B and minocycline,drug resistance rate ofAcinetobacter baumannii to the other antibiotics were relatively high (81.0％-100.0％).Pseudomonas aeruginosa was sensitive to polymyxin B but highly resistant to other antibiotics (57.7％-100.0％).Enterobacter cloacae was sensitive to imipenem and meropenem,while its drug resistance rates to ciprofloxacin,levofloxacin,cefoperazone/sulbactam,cefepime,piperacillin/tazobactam were 25.0％-49.0％,and those to the other antibiotics were 66.7％-100.0％.Drug resistance rates of Klebsiella pneumoniae to cefoperazone/sulbactam,imipenem,and meropenem were low (5.9％-15.6％,two imipenem-and meropenem-resistant strains were identified by modified Hodge test),while its drug resistance rates to amoxicillin/clavulanic acid,piperacillin/tazobactam,cefepime,eefoxitin,amikacin,levofloxacin were 35.3％-47.1％,and those to the other antibiotics were 50.0％-100.0％.(3) Drug resistance rates of methicillin-resistant Staphylococcus aureus (MRSA) to most of the antibiotics were higher than those of the methicillin-sensitive Staphylococcus aureus (MSSA).MRSA was sensitive to linezolid,vancomycin,and teicoplanin,while its drug resistance rates to compound sulfamethoxazole,clindamycin,minocycline,and erythromycin were 5.3％-31.6％,and those to the other antibiotics were 81.6％-100.0％.Except for totally resistant to penicillin G and tetracycline,MSSA was sensitive to the other antibiotics.Fourteen Staphylococcus aureus strains were resistant to erythromycin-induced clindamycin.Enterococcus was sensitive to vancomycin and teicoplanin,while its drug resistance rates to linezolid,chloramphenicol,nitrofurantoin,and high unit gentamicin were low (10.0％-30.0％),and those to ciprofloxacin,erythromycin,minocycline,and ampicillin were high (60.0％-80.0％).Enterococcus was fully resistant to rifampicin.(4) Fungi was sensitive to amphotericin B,and drug resistance rates of fungi to voriconazole,fluconazole,itraconazole,and ketoconazole were 7.2％-12.5％.(5) The mortality of patients with extremely severe burn was higher than that of patients with non-extremely severe burn.The variety of infected pathogens in patients with extremely severe burn significantly outnumbered that in patients with non-extremely severe burn (Z =-2.985,P =0.005).Conclusions The variety of pathogen in severely burned patients with bloodstream infection is wide,with the main pathogens as Acinetobacter baumannii,Staphylococcus aureus,and Pseudomonas aeruginosa,and the drug resistance situation is grim.The types of infected pathogen in patients with extremely severe burn are more complex,and the mortality of these patients is higher when compared with that of patients with non-extremely severe burn.

Objective To analyze the distribution and drug resistance of pathogens from the wounds of thermal burn patients,so as to provide reliable basis for the rational use of antibiotics and the effective control over nosocomial infection.Methods Wound samples of 1 310 thermal burn patients admitted into our burn wards from January 2012 to December 2017 were collected and retrospectively analyzed.API bacteria identification panels and automatical bacteria identification equipment were used to identify pathogens.E test was conducted to detect drug resistance of pathogens to vancomycin,tigecycline,and oxacillin.KirbyBauer paper disk diffusion method was used to detect drug resistance of pathogens to 31 antibiotics including penicillin G,gentamicin and rifampicin,etc.,and drug resistance of fungi to 5 antifungal agents (voriconazole,amphotericin B,fluconazole,itraconazole,and ketoconazole).The WHONET 5.6 software was used to analyze the constituent ratios of Gram-negative bacteria,Gram-positive bacteria,and fungi in each year;the distribution of fungi;the distribution of top 10 bacteria with the highest constituent ratios in each year;the constituent ratios of methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-sensitive Staphylococcus aureus (MSSA);the drug resistance of top 3 bacteria with the highest constituent ratios to commonly used antibiotics in each year;and the drug resistance of Candida to commonly used antifungal agents.Results (1) Totally 2 183 strains of pathogens were isolated for the first time,including Gramnegative bacteria 1 194 (54.70％) strains,Gram-positive bacteria 879 (40.27％) strains,and fungi 110 (5.04％) strains.From 2012 to 2016,the constituent ratio of Gram-negative bacteria showed a decreasing trend,while that of Gram-positive bacteria showed an increasing trend year by year;and the constituent ratio of fungi was with a significantly increasing trend from 2016 to 2017.(2) Among all the fungi,the constituent ratio of Candida parapsilosis ranked the first,Aflatoxin ranked the second,Candida albicans and Candida tropicalis both ranked the third.(3) From 2012 to 2017,top 10 bacteria with the highest constituent ratios,from high to low,were Staphylococcus aureus,Pseudomonas aeruginosa,Acinetobacter baumannii,Enterobacter cloacae,Escherichia coli,Staphylococcus haemolyticus,Klebsiella pneumoniae,Enterococcus faecalis,Aeromonas hydrophila,and Stenotrophomonas maltophilia respectively.The constituent ratio of Staphylococcus aureus ranked the first in each year.The constituent ratio of Pseudomonas aeruginosa was fluctuating but showed a rising trend comprehensively.The constituent ratio of Acinetobacter baumannii went up after decreasing.(4) Among all the Staphylococcus aureus,constituent ratio of MRSA was above 65.00％,while that of MSSA was below 31.00％ in each year.(5) From 2012 to 2017,Staphylococcus aureus resistant to vancomycin,linezolid,or teicoplanin was not detected;the drug-resistant rates of MRSA to penicillin G,oxacillin,gentamicin,rifampicin,tetracycline,ciprofloxaein,ofloxacin,and levofloxacin were above or equal to 80.0％ in each year;the drug-resistant rates of Staphylococcus aureus to clindamycin and erythrocin showed an obviously increasing trend,the drug-resistant rates of Staphylococcus aureus to moxifloxacin and queenoputin/daputin in 2017 were higher than those in 2016,while the drug-resistant rates of Staphylococcus aureus to the other 14 antibiotics showed no significant change in trend.From 2012 to 2017,Acinetobacter baumannii was sensitive to polymyxin B and tigecycline;the drug-resistant rate of Acinetobacter baumannii to ceftriaxone was relatively high;the drug-resistant rates of Acinetobacter baumannii to levofloxacin,minocycline,and tetracycline were decreasing while those to the other 14 antibiotics went up after decreasing.From 2012 to 2017,Pseudomonas aeruginosa wasn't resistant to polymyxin B,and its drug-resistant rates to the other 14 antibiotics showed decreasing trends.(6) The drug-resistant rates of Candida albicans to voriconazole,amphotericin B,fluconazole,itraconazole,and ketoconazole were all zero.The drug-resistant rates of non-Candida albicans to voriconazole,fluconazole,itraconazole,and ketoconazole were higher than those of Candida albicans.Conclusions Among the pathogens from the wounds of thermal burn patients,Staphylococcus aureus,Pseudomonas aeruginosa,and Acinetobacter baumannii had the top 3 constituent ratios;the constituent ratio of non-Candida albicans was obviously higher than that of Candida albicans.The high drug resistance rates of Staphylococcus aureus and Acinetobacter baumanni require more attention from clinicians and the local hospital's infection control department.

The burn microbiology laboratory of the author′s unit is a level Ⅱ biosafety laboratory, which is mainly responsible for handling clinical microbial samples from our department and other departments in the hospital. Since the outbreak of the coronavirus disease 2019, in order to ensure the normal operation of routine work and the safety of medical staff, the microbiology laboratory has actively adjusted the daily work flow. The detailed work flow is summarized as follows to provide references for the safety protection of peer in clinical microbiology laboratory.

Objective: To explore the resistance mechanism and gene type of carbapenems-resistant Klebsiella pneumoniae (CRKP) in burn care unit. Methods: A total of 27 CRKP strains were primarily isolated from 22 patients [20 males, 2 females, aged (42±16) years] admitted to burn care unit of Institute of Burn Research of the First Affiliated Hospital of Army Medical University (the Third Military Medical University, hereinafter referred to as our department) from January to December 2017. After identification of bacteria, the months of detection and distribution of sample source were analyzed. Drug resistance tests of 15 antibiotics were conducted. Polymerase chain reaction was used to detect the drug resistant genes. Pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST) were used to analyze the gene type of strains. Results: (1) During the whole year of 2017, CRKP strains were mostly detected in August (8 strains), September (6 strains), and October (5 strains), with no CRKP in January, March, June, November, and December. Five strains from bed units were detected in August (2 strains), September (1 strain), and October (2 strains). (2) Twenty-seven CRKP strains were derived from blood samples (40.7%, 11/27), wound exudate samples (18.5%, 5/27), deep vein catheter samples (11.1%, 3/27), sputum samples (7.4%, 2/27), urine samples (3.7%, 1/27), and bed unit samples (18.5%, 5/27). (3) The 27 CRKP strains were detected with drug-resistance rates of 100.0% to 7 antibiotics including cefoperazone/sulbactam, piperacillin/tazobactam, cefazolin, ceftriaxone, cefepime, ertapenem, and compound sulfamethoxazole, no drug-resistance to tigecycline, with drug-resistance rates higher than 81.0% to the rest 7 antibiotics. (4) Detection rates for resistance gene bla_CTX-M-10, bla_SHV, bla_TEM, bla_CTX-M-14, bla_ACT, and bla_KPC were all above 92.5%. (5) According to PFGE, the 27 CRKP strains had 6 types (A, A₁, A₂, B, C, and D). Strains of type A were mainly detected in February, May, and September, with detection rate of 37.0% (10/27). Strains of type C were mainly detected in July, August, and October, with detection rate of 48.1% (13/27). Strains of types A₁, A₂, B, and D were scatteredly detected, with detection rate of 3.7% (1/27) respectively. According to MLST, the 27 CRKP strains had 6 STs. ST11 was the most frequent type, accounting for 74.1% (20/27), which was detected in August to October. The detection rate of ST395, ST2230, ST215, ST260, and STnew ranged from 3.7%(1/27) to 7.4%(2/27), and the strains were scatteredly detected. Conclusions The main source of CRKP from burn care unit of our department was bloodstream. All the CRKP strains showed high drug-resistance rate and complicated resistance mechanism. There were small scale outbreaks caused by CRKP of type A, type C, and ST11, which should be paid more attention to in clinical treatment and infection control.