Objective To study salinomycin (Sal) enhance the sensitivity of liver cancer cell to 5‐fluorouracil (5‐FU) and its mechanism ,and to provide drug‐resistant primary hepatocellular carcinoma (HCC) patients with a new treatment .Methods Hepa‐toma cell line HepG2 ,SMMC‐7721 ,M HCC‐97H were used for the research .The effect of Sal combined with 5‐FU on the cell pro‐liferation inhibition rate ,colony formation ,apoptosis and tumor stem cell proliferation were detected by the M TT assay ,colony for‐mation assay ,flow cytometry .And the effect on Wnt/‐catenin when Sal combined with 5‐FU were detected by Western‐blot . Results Sal combined with 5‐FU significantly inhibited the liver cancer cell proliferation and colony formation and induced cell ap‐optosis ,showed the synergistic effect .5‐FU promoted the proliferation of hepatocarcinoma stem ,but Sal reduced the function of 5‐FU .At the same time Sal could inhibit the Wnt/‐catenin signal pathway .Conclusion The Sal can increase the sensitivity of hepato‐cellular carcinoma cells to 5‐FU by inhibiting Wnt/‐catenin signal pathway ;Sal combined with 5‐FU could provide drug‐resistant HCC patients with a new treatment .

[ ABSTRACT] AIM:To investigate the effect of insulin resistance ( IR) on the biological function of hepatocellu-lar carcinoma ( HCC) and sensitivity to cisplatin.METHODS:IR was induced in HepG2 cells via incubation with a high concentration of insulin.Afterwards, the effects of IR on adhesion, migration, invasion and sensitivity to cisplatin of the cells were detected.RESULTS:The results indicated that glucose consumption was reduced in the IR cells.The expres-sion of the insulin receptor and glucose transporter 2 was down-regulated.Furthermore, HepG2/IR cells displayed marked-ly enhanced adhesion, migration, and invasion.These cells exhibited a lower sensitivity to cisplatin.On the contrary, HepG2/IR cells exhibited decreased adhesion and invasion after treatment with the insulin sensitizer pioglitazone hydrochlo-ride.CONCLUSION:IR is closely related to drug resistance, adhesion, migration and invasion in HepG2 cells.These findings may help explain the clinical observation of the limited efficacy of chemotherapy on a background of IR.

Objective To observe the effect of integrated therapy of Chinese medicine and chemical drugs on adverse reaction and curative effect of initial treatment of secondary pulmonary tuberculosis. Methods Totally 1404 patients with secondary pulmonary tuberculosis and TCM lung consumption diagnostic criteria (syndrome of lung yin deficiency, qi-yin deficiency, yin-deficiency caused excessive fire) were chosen for single blind, randomized, controlled, multicenter clinical trials. Trial group was given 2HRZE/4HR, 1 time/day with Chinese medicine 2 or 3 times/day, and control group was given 2HRZE/4HR only for six months. The adverse reactions and clinical symptoms were observed to evaluate clinical efficacy and safety. Results In terms of reducing liver damage and other adverse reactions, the ratio of trial group had statistical difference with that of control group (P<0.001). In symptom scores of lung yin deficiency syndrome treated for 2, 4, 6 months, yin-deficiency caused excessive fire syndrome treated for 6 months, qi-yin deficiency syndrome treated for 4, 6 months, the differences between the two groups were significant (P<0.001). TCM syndrome curative effect between the two groups was statistical different (P<0.001). Safety evaluation result between the two groups was statistical different by tratified analysis (P<0.001). Conclusion Integrated therapy of Chinese medicine and chemical drugs can improve the symptoms and reduce adverse reactions caused by chemical drugs. It can enhance the curative effect and safety.