Objective To assess the effect of existing cleaning methods applied in cardiac surgical intensive care unit, analyze potential danger and provide evidence to support clean processes in the future. Methods Each patient′s bed unit was defined as a sample and the frequency of hand-to-bed contact and the extent of patient-to-bed contact were assessed.Totals of 15 monitoring surface on each sheet were taken after sterilization within 24 hours and the Relative Light Units( RLU) of the monitoring surface values were compared with Adenosine Triphosphate ( ATP) bioluminescence techniques.Results RLU values were more than 500 on bedside table surface, bed block, ECG buttons,micro pump surface, pressurized infusion ball surface and crank bed.RLU values were between 250 and 500 on nurses′small table surface, breathing machine buttons, rulers and stethoscope surface.RLU values were less than 250 on treatment plate, footboard, pump lines and vacuum tube surface.Conclusions Hand hygiene plays a significant role in daily life.It is important to strengthen routine hand hygiene publicity and education, and monitoring and supervision can be used as an effective measure to improve the surface cleanliness of objects.