Objective To analysis the clinical features of mycoplasma pneumoniae nucleic acid(MP-DNA)testing positive cases of from the region's children,provide the basis for clinical diagnosis and treatment.Methods Outpatient and hospitalized patients with respiratory infection symptoms were sampled pharyngeal swab in the region from 2014 to 2015.After DNA was extracted,polymerase chain reaction method was conducted to detect the MP gene.At the same time,blood routine and mycoplasma pneumoniae antibody were detected from drawing blood.Results There were 209 cases of MP-DNA positive from 2018 cases in children with respiratory tract infection,the positive rate was 10.36 ％.MP-DNA positive rate in 3-＜ 14 years old age group was 18.25 ％,which was higher than other age groups(P＜0.05).The positive rate was 16.11％ from July to September,which was higher than in other month groups(P＜0.05).The positive rate of girl (13.04 ％) was significantly higher than that of boy (8.79 ％) (P＜ 0.05).WBC count was higher than normal for 22.01 ％,normal accounted for 49.28 ％,lower than normal for 28.71％ in MP-DNA positive children.Serum MP-IgG positive rate was 69.86％ in MP-DNA positive children.Conclusion MP-DNA testing has important value to the pediatric respiratory infection diagnosis,detection of multiple projects has important significance to preventing illness aggravating.

Objective To investigate the effect of acute or chronic administration of eseitalopram(ESC)on behavior of mice,which suffered from chronic unpredictable mild stress(CUMS).Methods Mice were exposured to CUMS for 8 weeks,then the behavior of mice were investigated every 2 weeks,and the effect of acute and chronic administration of escitalopram on behavior was compared.Results (1)The body weight of all stressed mice were lighter than control group(18.5±1.1)g from 2nd to 8th week(P＜0.01).After chronic administration of escitalopram,the body weight of cms+Cesc group(18.5±0.6)g were increased relative to other stressed groups(P＜0.01).(2)In open-field test for 15min,the total distance and central distance of stressed groups were further,and numbers of rear were more than control group(P＜0.05)from 2nd to 4th week,but then these indexes were decreased from 6th to 8th week(P＜0.05).After chronic administration,the total distance(57.4±11.0)m,central distance(12.0±3.0)m and numbers of rear(74.2±6.1)of cms+Cesc group increased(P＜0.05).(3)In sucrose preference test,the preference percentage of stressed groups was less than control group(P＜0.05)from 6th to 8th week at 8th week,the preference percentage of cms + Cesc group((79.0±2.7)%)was more than other stressed groups(P＜0.05).(4)In forced swimming test,compared with control group,immobility time of stressed groups became longer from 4th to 8th week..After chronic administration,immobility time of cms + Cesc group((124.7±8.00)s)was shorter than other stressed groups.(5)After acute administration,the behavior of cms + Aesc group was not different relative to pure stressed group(P＞0.05).Conclusion Chronic administration of escitalopram show up efficacy on depression behavior of CUMS mice,but acute administration is ineffective.

Objective To summarize the published studies of diabetic cerebral infarction treated by integration of traditional and western medicine (ITWM) or pure western medicine and compare their clinical curative effects. Method The clinical study of ITWM for diabetic cerebral infarction were collected and seleted according to the standard. The effect of ITWM and only western medicine was compared, and Meta-analysis was made. Result 59 studies were included to analysis neurological deficit and the total efficiency. Meta-analysis revealed that the improvement of ITWM is better than pure western medicine treatment. Conclusion The existed limited evidences suggest that ITWM treatment of diabetic cerebral infarction can significantly improve neurological deficit and curative effect. More high-quality randomized, double-blind, placebo-controlled trials are needed to confirm the efficacy and safety of ITWM treatment of diabetic cerebral infarction.

Background: The quality of life has been greatly influenced and the cost of medical expenses is very high in patients with irritable bowel syndrome (IBS). The etiology and pathogenesis of IBS are still unclear, and the prevention and treatment of this disease still lack of effective methods. Objective: To explore and analyze the effects of Tiaohe Ganpi Hexin Decoction (TGHD), a compound traditional Chinese herbal medicine for regulating the liver and spleen, on IBS patients with diarrhea. Design, setting, participants and interventions: All 40 IBS patients came from the First Hospital of Jinan University, Guangzhou Red Cross Hospital, and the First Affiliated Hospital of Guangzhou University of Chinese Medicine, and were randomly divided into two groups. Patients in the treatment group (n=20) were given TGHD, while those in the control group (n=20) were prescribed oral pinaverium with a four-week treatment period. Main outcome measures: Traditional Chinese medicine (TCM) syndrome score, total obviously effective rate, disappearance rate of symptoms, and clinical symptom score in the two groups were evaluated before and after four-week treatment. Results: After the treatment, TCM syndrome scores in both groups were decreased (P<0.01), and the TCM syndrome score in the treatment group was significantly lower than that in the control group (P<0.01). There was a significant difference in the total obviously effective rate between the two groups (P<0.01), and the total obviously effective rates in the treatment and control groups were 85%(17/20) and 45%(9/20) respectively. The disappearance rates of abdominal pain, abdominal distention, poor stool output, stool frequency, stool character and mucous stool in the treatment group were higher than those in the control group (P<0.05). The symptom scores of abdominal pain, abdominal distention, poor stool output, stool frequency, stool character and mucous stool in the treatment group were lower than those in the control group (P<0.05). Conclusion: TGHD can significantly improve the clinical symptoms in IBS patients with diarrhea.

Objective To investigate the diagnostic value of conventional and dynamic gadolinium-enhanced T1-weighted (T1W) MRI in benign and malignant endometrial polypoid lesions. The pathologic basis of MRI features was also evaluated. Methods The conventional and dynamic contrast enhanced MRI features in 48 cases with clinicopathologically-proved endometrial polypoid lesions were retrospectively analyzed, including 26 cases with malignant tumors (23 eases with endometrial carcinoma and 3 cases with endometrial sarcoma) and 22 cases with benign polypoid lesions (12 cases with endothehal polyps and 10 cases with submucons leiomyomas). The occurrence probability of central fibrous core, intratumoral cysts and intact junctional zone were evaluated by Fisher's exact test. Results Benign and malignant endometrial polypoid lesions had specific characteristics on MRL Endometrial carcinomas usually appeared as homogeneous intermediate or slightly high signal-intensity masses on T2-weighted images, and relatively homogeneous hypedense masses on contrast-enhanced images with myometrial invasion (P < 0.05). An irregular central fthrous core and small non-enhanced intratumoral cysts were seen more frequently in endometrial polyps (P < 0.05) than others. All 3 cases with uterine sarcomas showed obviously inhomageneous intensity on T2WI and demonstrated areas of early and persistent marked enhancement.Submucous leiomyomas demonstrated isodense or slightly low-density masses with defined outline on T2 WI and the endomerium was displaced. The diagnostic sensitivity, specificity and accuracy of malignant polypoid masses were 92.3% (24/26), 83.3% (20/24) and 95.8% (46/48) respectively. Conclusion Conventional and dynamic contrast-enhanced MRI are valuable in characterizing the benign and malignant endometrial polypoid lesions.

ObjecfiveTo compare the sensitivity of diffusion weighted imaging(DWI)with dynamic contrast enhanced(DeE)MRI for the detection of small breast caJlcers and to evaluate the clinical value of DWI.MethodsForty-eight patients with benign(n=25)and malignant(n=45)small breast lesions(≤2 cm)proved by pathology underwent DWI and DCE MRI.The DCE MRI was performed using FLASH sequence and the time-signal intensity chive was drawn.The DWl was performed using GRAPPAEPI sequence with different b values(800,1000 s/mm2) and the ADC values of lesions were measured.The sensitivity and specificity of DWI for the detection of small breast cancers were compared with DCE MRI. ResultsForty of 45 small breast cancers and 19 of 25 small benign breast lesions were corrlectly diagnosed using DCE MRI.The sensitivity and positive predictive value of TIC were 88.9%(40/45)and 87.0%(40/46).With b values of 800 s/mm2 and 1000 s/mm2,the avemge ADC values of small breast cancers were(1.153±0.192)× 10-3 and(1.079±0.186)× 10-3 mm2/s,while those of benign ones were (1.473±0.252)×10-3 and(1.419±0.255)×10-3 mm2/s,respeetively. There was no significant difference for the ADC values with different b values in the same group(P＞0.05),while there was a signiticant difference between the malignant and the benign lesions(P＜0.05)Thirty-nine of 45 small breast cancers and 19 of 25 small benign breast lesions were correctly diagnosed using DWI with b value of 1000see/mm2.Both the sensitivity and positive predictive value of diagnosis were 86.7%(39/45).The abilities of DWI and DCE MRI for the diagnosis of small breast cancers werle the same. The sensitivity(93.3%)and positive predictive value(91.3%)were improved with the combination of DCE MRI and DWI. Conclusion DWI has a high sensitivity for the detection of small breast cancers,the ADC value can provide valuable information in the differential diagnosis.

Objective To investigate the effects of low dose radiation on dendritic growth of newborn neurons in the hippocampus of young rat.Methods One month-old male rats were randomized into radiation group aind sham control group.Radiation group received whole brain irradiation at a single dose of 2 Gy.Retrovirus expressing green fluorescent protein (GFP) was used to label newborn neurons in the hippocampus through stereotaxic intracranial infusion.Immunofluorescence assays were performed to detect dendritic architecture alterations induced by irradiation at different time points.Results Compared with control group,the lengths of total dendrite and the longest dendrite significantly decreased at 2 and 4 weeks after irradiation (t =3.10,2.07,2.94,4.02,P ＜ 0.05).The branching points of new born neurons were also decreased significantly at 2 weeks post irradiation (t =2.23,P ＜ 0.05).The number of new born neurons reduced at 4 weeks post irradiation (t =8.43,P ＜ 0.05).Conclusions Low dose radiation could inhibit newborn neuron growth in the hippocampus of young rat,which may be one of the most important mechanisms involved in radiation-induced cognitive impairment.

A Ni-based rare-earth perovskite LaNiTiO3 nanoparticles was synthesized and its catalytic activity was investigated. Based on this, a simple and quick nonenzyme electrochemical sensor was fabricated with stable and reliable performances for the determination of hydrogen peroxide (H2 O2 ). The techniques of X-ray diffraction, FT-IR spectra, transmission electron microscopy, X-ray fluorescene spectroscopy and scan electronmicroscope were used to characterize the composition, structure and morphology of as-synthesized sample. The sensor based on this nanomaterial was investigated and optimized by cyclic voltammetry and current-time techniques. The results showed the working electrode modified with LaNiTiO3 (0. 5 g / L, 8. 0μL) in 0. 1 mol/ L NaOH exhibited good catalytic properties for H2 O2 . Under the optimum conditions, the sensor performed excellent properties, such as quick response time ( about 2 s ), a wide linearity (0. 2 μmol/ L -8. 0 mmol/ L), a low detection limit of 0. 05 μmol/ L ( S / N = 3 ), a high sensitivity of 957 μA (mmol/ L) -1 cm-2 , good reproducibility and anti-interference ability, which was better than those of some other biosensors reported recently. So, it may be used for the analysis and detection of H2 O2 in practical samples such as biomedicine.

OBJECTIVE:To investigate the characteristics and general rule of ADR induced by Ginkgo leaf extract and dipyri-damole injection,and to provide reference for clinical rational drug use. METHODS:UsingGinkgo leaf extract and dipyridamole injectionADRas subject,the journal articles were retrieved from CJFD during Jan. 1st,2005-Jun. 28th,2016,and then ana-lyzed statistically in respects of gender,age,primary disease,allergic disease,drug use,occurrence time of ADR,organs/systems involved and clinical manifestations. RESULTS:A total of 14 valid articles had been collected,involving 727 patients in total. Meanwhile,female was more than male(57.63% vs. 42.37%)and most of them aged more than 50 years;primary diseases were mainly thromboembolic disease and coronary heart disease;most of ADR happened within 30 min after medication (268 cases, 36.86%). Organs/systems involved in ADR were mainly nervous system (254 cases,28.60%),followed by skin and its appen-dants(228 cases,25.68%),digestive system(187 cases,21.06%);severe ADR could cause anaphylactic shock. There were 18 cases of new severe ADR (2.48%);all ADR cases were recovered,and no death occurred. CONCLUSIONS:It is suggested to strictly control indications,differential diagnosis and treatment,rational drug use,close monitoring through the whole process, maintain a high level of awareness to ADR.

Objectives: To evaluate the efficacy, toxicity and side effects of CAP (cytoxan, adriamycin, cisplatin), NP(navelbine, cisplatin), GP(gemcitabine, cisplatin) in the treatment of patients with non small cell Lung cancer(NSCLC). Methods:146 patients with NSCLC diagnosed by pathology or cytology were treated with CAP, GP or GP regimen. Results:The overall response rate of the three groups (CAP, NP, GP)were 33.33%, 46.43% and 47.92% respectively. The major toxicity and side effects was bone marrow inhibition and the gastrointestinal reaction. Grade Ⅲ-Ⅳ side effects of CAP group were significant higher than the others. Conclusions:The regimen of navelbine and cisplatin or gemcitabine and cisplatin was more effective and less toxic than cytoxan, adriamycin, cisplatin and cisplatin.