Nonalcoholic fatty liver(NAFL)is characterized by the hepatocyte steatosis and lipid accumulation,and is a clinical syndrome of unrelated to excessive alcohol consumption. At present,patients with the syndrome obviously increase in the whole world. Sterol regulatory element-binding proteins (SREBPs) are the key transcription factors of controlling the biosynthesis of cholesterol,fatty acids and triglycerides,and adipocyte differentiation. In this paper,the regulation of SREBPs and its research progress in the development of NAFL were reviewed.

Aim To establish and evaluate a mouse model of my-ocardial fibrosis by hypodermic injection of isoprenaline. Meth-ods Kunming mice were randomly divided into 2 groups, namely, the myocardial fibrosis model group and the control group, 10 mice in each group. The mice in the model group were given isoprenaline 5 mg · kg-1 by hypodermic injection. From the following day, the dose of isoprenaline was reduced to 2. 5 mg·kg-1 , and lasted for 30 days. The mice in the control group were treated with the physiological saline in the same way. Final-ly, heart weight was then weighed and the cardiac weight index (CWI) was calculated. Hydroxyproline (Hydro) level in myo-cardium was determined by a colorimetric method. HE and Mas-son’s trichrome staining were used to estimate the extent of myo-cardial fibrosis and calculate the collagen volume fraction ( CVF) . RT-PCR was used to measure the myocardial Collagen I mRNA expression. Results Compared with the control group, the CWI and Hydro content in the myocardial tissues in the model group were increased(P<0.01). The content of col-lagen in the myocardial tissues and the CVF were increased obvi-ously(P<0.01). The RT-PCR results showed that the left ven-tricle Collagen I mRNA expression in the model group increased obviously( P<0.01 ) . Conclusion Isoprenaline-induced myo-cardial fibrosis model has been established and the method is very simple, economic and reliable.

Aspirin is one of the major drugs used for preventing atherothrombotic vascular diseases,but not effective to all patients or so-called aspirin resistance.However,the mechanisms of this resistance still remains to be elucidated.The recent progresses is reviewed in the therapeatic effect of aspirin and mechanisms of the drug resistance.

Literatures available was reviewed on the thrombosis and mechanism of new antithrombotic agents.The pathophysiology of the thrombosis involves dysfunction of endothelium and cell adhesion.The new antithrombotic drugs include factor Ⅹa inhibitors, GPⅡb/Ⅲa receptor blocking agents and the drugs modulating the function of endothelium.

Objective To study the antioxidation and immuno-regulation effects of the active fraction in Jiawei Zhisou Powder.Methods Hypersensitive asthma mice were established by the miscible liquids of ovalbumin(OA)and Al(OH)3.The influences of the powder on the contents of SOD and MDA in the serum and lung tissue of mice were observed and the contents of IL-4 and IFN-?in supernatant of mouse spleen cells cultured in vitro were measured by enzyme-linked immunosorbent assay.And Jiawei Zhisou Powder and dexamethasone were administered to normal mice respectively,then the immune organs weight were measured and the spleen and thymus indexes of mice were calculated.Results Compared with the normal group,the contents of SOD and IFN-?were significantly decreased,while MDA and IL-4 contents were obviously enhanced in asthma group(P

AIM:To investigate the protective effect of ginger oil on liver.METHODS:Mouse model of acute hepatic injury induced by carbon tetrachloride and acetaminophen were used to observe the protective effect of ginger oil on liver.RESULTS:Ginger oil could obviously decrease ALT and AST levels of mouse model of acute hepatic injury induced by carbon tetrachloride and acetaminophen,significantly decrease MDA level of rat liver tissue and mouse serum(P

Aim To investigate the effect of osthole against thrombosis and platelet aggregation.Methods Rat model of artery-vein bypass thrombosis and mouse model of thrombosis by injecting collagen-adrenaline to the vein of the tail were used to measure the thrombus weight and to observe the number of dead mice in 5 min and the recovery mice from hemiplegia in 15 min. ADP,thrombin and arachidonic acid were used to induce platelet aggregation on human and the rate of platelet aggregation in 1,3 and 5 min and the maximum rate of platelet aggregation were detected.Results Osthole inhibited artery-vein bypass thrombosis and reduced thrombus weight in rats to defferent degrees,inhibited the thrombosis induced by collagen-adrenaline and reduced the death rate in 5 min and increased the recovery rate in 15 min. Osthole also inhibited human platelet aggregation induced by ADP,thrombin and arachidonic acid in vitro.The values of IC_(50 )(dose of the drug giving 50% inhibition) were 0.444 g?L~(-1) for ADP,0.186 g?L~(-1) for thrombin, 0.421 g?L~(-1) for arachidonic acid, respectively.Conclusion Osthole exert remarkable effects against thrombosis and platelet aggregation.

Hyperlipidemia is a major risk factor for the generation and development of atherosclerosis and coronary heart disease. The lipid-lowering effects of drugs were mediated by the control of lipid metabolism.Recently some new targets in the process of lipid metabolism were found,they may lead to the development of new drugs.