Objective To investigate the college students′understanding of antimicrobial agents knowledge in two colleges of Huainan and provide a scientific basis to further conduct the health education of rational use of anti -microbial drugs among college students .Methods The antimicrobial perception of 2 college students with 10 differ-ent majors were investigated by cluster-sampled with the questionnaires designed by the authors .Results The correct answer rate of antimicrobial agents knowledge was 61.7%in overall college students ,72.3%in pharmaceutical pro-fessional class students,53.4%in non-medical specialty,and the difference was statistically significant (P<0.01). In lower grade students,the rate was 57.2%and 68.1%in high school students.Also the difference was statistically significant (P<0.01).The rate of students who use antimicrobial drugs without guidance reached 84.1%,the rate in pharmaceutical professional class students (88.6%) was higher than that in non-medical professional class students (80.6%) (P<0.01).Conclusion College students are not sufficiently aware of using antibiotics reasonably and adverse reaction .Consequently ,related publicity and education should be strengthened in various forms to improve lev-el of using antibiotics reasonably among college students .

Objective to investigate the relationship between CRP,IL-12 and gestational diabetes mellitus.Methods Fifty normal pregnant women (NGT group) and forty with GDM (GDM group) were enrolled in the study.The serum IL-12、fasting blood sugar、CRP were mesured respectively.Results GDM fasting blood glucose,fasting insulin,insulin resistance index (IRI),CRP,IL-12 were higher than those in the control group [(4.95 ± 0.76 vs 4.57 ± 0.58) mmol/L,(13.65 ± 1.74 vs 8.78 ± 1.65) uIU/ml,(2.91 ± 1.05vs 1.72 ± 0.87),(5.56 ± 1.24 vs 3.25 ± 1.13) mg/L,(71.47 ± 4.35 vs 56.03 ± 4.06) PG,/ml,P ＜ 0.05),the results of single factor analysis of CRP showed a positive correlation with IRI (r =0.865,P ＜ 0.05),IL-12 was positively correlated with IRI (r =0.913,P ＜ 0.05).Conclusions Our study show inflammatory cytokins take part in the genesis of gestational diabetes.Serum levels of IL-12 and is one factor in pathogensis of GDM.

AIM:To investigate the protective effect of ginger oil on liver.METHODS:Mouse model of acute hepatic injury induced by carbon tetrachloride and acetaminophen were used to observe the protective effect of ginger oil on liver.RESULTS:Ginger oil could obviously decrease ALT and AST levels of mouse model of acute hepatic injury induced by carbon tetrachloride and acetaminophen,significantly decrease MDA level of rat liver tissue and mouse serum(P

Objective To study the serum level of resistin and IL‐6 in the sepsis‐induced acute lung injury (ALI) in rats and to probe the relation of IL‐6 and resistin to ALI and its possible mechanism .Methods The forty‐five Wistar rats were randomly as‐signed to three groups ,a control group ,a sham group and a model group .ALI was induced by intravenous injective of LPS (10 mg/kg) and those animals were killed after 6 hours ,12 hours ,24 hours .The lung wet/dry weight ratio ,serum IL‐6 was detected by chemiluminescence and resistin levels were detected by ELISA .Results Compared with the control group and the sham group ,the lung wet/dry weight ratio ,lung pathologic tissue score ,the serum level of resistin and IL‐6 were increased(P<0 .05) .Positive cor‐relation was observed between resistin and IL‐6 in different time .Conclusion Resistin possibly participate the process of sepsis in‐duces ALI .

Objective:To investigate the expression and clinical significance of cell division cycle 42 (Cdc42) and WASP family verprolin-homologous protein l (WAVE1) in non-small cell lung cancer (NSCLC). Methods:The expression of Cdc42 and WAVE1 was detected in 106 paraffin-embedded NSCLC tissues and 46 adjacent normal lung tissues (control group) using immunohistochemis-try. Results:The expression levels of Cdc42 and WAVE1 was distinctly higher in NSCLC than in the control group. The expression of Cdc42 in NSCLC significantly correlated with tumor differentiation, TNM stage, and lymph node metastasis (P<0.05). The expression of WAVE1 in NSCLC was significantly correlated with TNM stage and lymph node metastasis (P<0.05 or P<0.01). The expression of Cdc42 was significantly correlated with WAVE1 in NSCLC (r=0.469, P<0.01). The 3-year survival rates were significantly lower in the group with high Cdc42 expression (44.16%) than in the low expression group (72.41%;P<0.01). Similarly, the 3-year survival rates were significantly lower among patients with high WAVE1 expression (39.44%) than in those with low expression (77.14%;P<0.01). Lymph node metastasis and the common high Cdc42 and WAVE1 expression were independent prognostic factors for NSCLC. Conclu-sion:The Cdc42 expression is correlated with WAVE1 expression. They may act together and have an important function in NSCLC. The expression of both Cdc42 and WAVE1 in NSCLC tissue may be used as markers for assessing the clinicopathologic features and prognosis.

Objective To investigate the relationship between ambulatory arterial stiffness index (AASI) and left ventricular mass index (LVMI) in the elderly hypertensive patients.Methods This study population consisted of 332 elderly hypertensive patients,who hospitalized in our department from January 2012 to December 2014.AASI was calculated from 24 h ambulatory BP monitoring recordings and LVMI from echocardiography examination.According to the median value of AASI,patients were divided to less than the AASI median group (low AASI group) and equal to or above the AASI median group (high AASI group).Differences between two groups were evaluated using the Student's t-test and Chi-square test.Univariate association was assessed by the Pearson correlation analyses.Multivariate linear regression models were performed to analyze the correlation between AASI and LVMI.Results LVMI was significantly higher in high AASI group compared with low AASI group ((115.91 ±21.36) g/m2 vs.(104.11 ± 17.24) g/m2,P =0.008).Pearson correlation analyses showed that AASI and 24 h pulse pressure were positively correlated to LVMI (r =0.332,P ＜ 0.001;r =0.169,P =0.002).In multivariate linear regression model,AASI(β =44.48,P＜0.001),LDL-C(β =-5.97,P＜0.001) and UA (β =0.02,P =0.045) showed significant association with LVMI.Conclusion AASI independently associated with LVMI,and AASI might be one predictor of left ventricular hypertrophy in hospitalized elderly hypertensive patients.

Objective:To observe the efficacy of Dabigatran etexilate anticoagulation therapy and analyze its influencing factors in elderly patients.Methods:The clinical data of the elderly patients using Dabigatran etexilate anticoagulation for prevention or treatment of thrombosis in the Department of Geriatrics, Peking University First Hospital were collected.Changes of coagulation indexes and its influencing factors were analyzed when taking Dabigatran in different doses.Results:A total of 142 patients receiving Dabigatran therapy were collected, with an average age of(79.0±7.5)years.Among them, 128 patients had atrial fibrillation and 14 had deep venous thrombosis and/or pulmonary embolism.After taking Dabigatran, the trough values of activated partial thromboplastin time(APTT)and thrombin time(TT)were increased significantly in all patients compared with that before administration( P<0.001). In patients taking Dabigatran 110 mg once a day, APTT was(32.4±3.0)s before administration, with a trough value of(41.4±5.4)s and a peak value of(53.9±9.8)s.In patients taking Dabigatran 110 mg twice a day, APTT was(31.0±3.1)s before administration, with a trough value of(42.4±5.5)s and a peak value of(48.7±7.3)s.Compared with patients taking Dabigatran 220 mg/d, those who took Dabigatran 110 mg/d were older( P<0.0001), often women( P<0.0001), had lower body weight( P=0.001), body mass index( P=0.018)and creatinine clearance rate( P<0.001), and higher basal( P=0.008)and peak values( P=0.002)of APTT, but with similar trough values.Multivariate analysis showed that the APTT trough values were significantly related with APTT basal value( β=0.885, P<0.001), creatinine clearance rate( β=-0.121, P<0.001), dosage of Dabigatran( β=0.037, P<0.001)and combined amiodarone( β=3.784, P=0.022). Conclusions:There are individual differences in the anticoagulant effect of Dabigatran etexilate.The APTT trough value is higher in patients with high APTT basal value, high Dabigatran dose, low creatinine clearance rate and combined use of amiodarone.Dabigatran 110 mg/d was often used in women, elderly patients, and those with low body weight, and decreased renal function, and with a similar trough value and a high peak value of APTT compared with patients on Dabigatran 220 mg/d.

Objective:To investigate acute adverse events and pregnancy outcome after vaccination of inactivated COVID-19 vaccine in the first trimester of pregnancy.Methods:The retrospective-prospective cohort study was conducted among pregnant women of 11-13 +6 weeks of gestation who visited the obstetric clinics for prenatal check in Lianyungang Maternal and Child Health Hospital from May to November in 2021, after registration for perinatal health cards in the community. Those who met the inclusion criteria were recruited and were divided into vaccination group and non-vaccination group according to whether they received inactivated COVID-19 vaccine in the first trimester. Women in the vaccination group were further divided into 1-dose group and 2-dose group. Information, including pregnancy-related screening, pregnancy complications, pregnancy outcome and acute adverse events, were collected and compared with independent samples t-test or ANOVA, Kruskal- Wallis H test or Mann-Whitney U test, χ2 test or Fisher's exact probability method. Results:Totally, 105 pregnant women were analyzed in 1-dose group, 90 in 2-dose group, and 194 in non-vaccination group. (1) There were no statistically significant differences in the occurrence of acute adverse events [1-dose group: 2.86% (3/105); 2-dose group: 6.67% (6/90); non-vaccination group: 4.63% (9/194); χ2=1.59; vaccination group was 4.61% (9/195), when compared with non-vaccination group, χ2=0.00], abnormal pregnancy-related screening indicators and abnormal pregnancy outcome among the three groups (all P>0.05), neither between the vaccination and non-vaccination group (all P>0.05). The acute adverse events in these women included fever, pain at the inoculation site, fatigue, local induration and rash.(2) The differences in hypertensive disorders in pregnancy among the three groups were statistically significant [1-dose group: 10.5%(11/105); 2-dose group: 17.8%(16/90); non-vaccination group: 7.7%(15/194); χ2=6.46, P=0.040], and the incidence was higher in the 2-dose group than that in the non-vaccination group (adjusted by Bonferroni, P<0.017). (3) Regarding other pregnancy complications, no difference was found among the three groups (all P>0.05), neither between the vaccination and non-vaccination group (all P>0.05). Conclusion:The risk of acute adverse events and adverse pregnancy outcome is similar in pregnant women who received inactivated COVID-19 vaccine versus those who did not in the first trimester, and regular blood pressure monitoring is recommended for those who received two doses of inactivated COVID-19 vaccine.

Objective:To explore the clinicaleffect of radial arterial puncture cannulationunder ultrasonic guidance in patients with critical diseases.Methods:From December 2016 to May 2017,120 patients under critical conditions in Department of Intensive Care Unit,Xiangya Hospital,Central South University,who received arterial cannulation,were randomly divided into 2 groups:a control group (traditional blind puncture method) and an observation group (ultrasound-guided radial arterial cannulation) (60 cases in each group).The success ratio of radial arterial puncture cannulation by one time,total success ratio of radial arterial puncture cannulation,numbers of puncture,the rate of complications and the time of consumption were compared between the 2 groups.Results:There was no statistically significant difference in the total success ratio of radial arterial puncture cannulationin the 2 groups (P＞0.05).The success ratio radial arterial puncture cannulation by one time was significantly greater in the observation group than that in the control group (P＜0.05);the numbers of puncture,the rate of complications and the time of consumption in the observation group were lower than those in the control group,with statistically significant (all P＜0.05).Conclusion:The application of ultrasound-guided radial arterial puncturecannulation can improve the success ratio of radial arterial puncturecannulation by one time,decrease the numbers of puncture,reduce the incidence of complications and save operation time.

The clinical evidences of the guideline came from clinical trials based evidence-based medicine. Applied principle of the evidence was: systematic reviews, RCTs, the results from multiple factors ana-lysis, consensus, especially combined with Chinese experience and some lung cancer guidelines used in USA or Europe. All doctors who use the guideline in making therapeutic strategy must combine patients' conditions with the knowledge of biological behavior, dynamic change and response to treatment of lung cancer.