Objective To understand the situation of sexual behavior and STD in floating population attending STD clinics Methods Floating population in STD clinics were investigated by questionnaires.Results Sexual behavior rate was 60.0% in unmarried floating population.Sexual behavior rate was 47.6% before marriage at the first sexual time.46.8% of the patients had more than 2 sex partners,and the rate of condom use among them was under 50%.STD prevalence rate was 37.1%,Re-infection rate was 12.2% accounting for 32.8% in all investigated STD patients.Conclusions It is importent to standardize service in STD clinics,provide health information,raise prevention knowledge,change their sexual attitude,advocate safety sexual behavior in various ways in high risk groups.

Objective To investigate the genetype distribution of mycoplasma pneumoniae(MP) by denaturing high-performance liquid chromatography(DHPLC).Methods A total of 300 cases nasopharyngeal aspirate were collected from our hospital.The MP genes of standard strains and clinical specimens isolates were amplified by PCR followed by DHPLC and genetype determination.Results A total of 110 cases were positive after 24 hours fermentation from 300 cases with pharyngeal swab.By the specific primers of MP-129,MP-FH standard strain and specimens,2 280 bp and 2 580 bp gene fragments were made out respectively.One hundred and ten strains of clinical isolates were detected by DHPLC.One hundred and seven strains of P1-Ⅰ were 1b subtype,3 were type P1-Ⅱ which were all 2a subtype.Conclusion The genetype of MP infection in children from our hospital is P1-Ⅰ,1b subtype by using DHPLC technology.

The herpes simplex virus type 1 (HSV-1) VP22, is one of the most abundant HSV-1 tegument proteins with an average stoichiometry of 2 400 copies per virion and conserved among alphaherpesvirinae. Many functions are attributed to VP22, including nuclear localization, chromatin binding, microtubule binding, induction of microtubule reorganization, intercellular transport, interaction with cellular proteins, such as template activating factor I (TAF-I) and nonmuscle myosin II A (NMIIA), and viral proteins including tegument protein VP16, pUS9 and pUL46, glycoprotein E (gE) and gD. Recently, many novel functions performed by the HSV-1 VP22 protein have been shown, including promotion of protein synthesis at late times in infection, accumulation of a subset of viral mRNAs at early times in infection and possible transcriptional regulation function.

Functional imaging plays an important role in lung cancer radiotherapy.Functional imaging techniques include single photon emission computed tomography,positron emission tomography,magnetic resonance imaging,and computed tomography.Using lung function information provided by these techniques for lung cancer radiotherapy can better protect the normal function of lung tissue,optimize the radiotherapy program,and reduce the incidence of radiation pneumonitis.With the development of perfusion and imaging technology,functional imaging technology is expected to be widely used in the precise radiotherapy of lung cancer in the future.

Objective To prepare an influenza A(H1N1) split-virus vaccine and observe its safe-ty and effectiveness. Methods According to the process for preparing seasonal flu split-virus vaccine two batches of vaccine were prepared with the flu A (H1N1) vaccine virus strain recommended by WHO. The pilot products were tested against the requirements of flu split-virus vaccine. Results The quality of the pi-lot vaccine has been tested by National Control Laboratory and conformed to the requirements. Nine hundred and sixty volunteers received one dose of vaccine containing either 15 μg or 30 μg of hemagglutinin. The re-suits indicated the both seroconversion rate and protection rate were higher the 70%. The GMT of HIAb of the volunteers who received 1 dose of 15 μg increased significantly by 15, 39, 37 and 25 times compared to those before vaccination in the age groups of 3-11, 12-17, 18-59 and ≥60, respectively. And 26, 72, 68 and 36 times rise were found in the postvaccinated volunteers of 30 μg group. The total adverse reaction rates of 15 μg and 30 μg dose group were 29.38% and 43.75%, respectively. The grade 2 adverse reaction rates of 15 μg and 30 μg dose group were 6.25% and 15.42%, and the grade 3 adverse reaction rates of 15 μg and 30 μg dose group were 0.83% and 1.46%, respectively. No serious adverse reactions were found. Conclusion The influenza A (H1N1) split-virus vaccine prepared according to the requirements of season-al flu vaccine is safe and effective.

Objective To evaluate the difference between stroke volume measured by electrical cardiometry-Icon (Osypka Medical, Berlin, Germany) and pressure recording analytical method-MostCare (Vygon, Vytech, Padova, Italy), and to assess their nursing demand, safety and sutibility.Methods From March to September in 2016, twenty-six infants undergoing open heart surgery using CPB were enrolled. After helping anesthesiologist to complete general anesthesia, invasive arterial pressure monitoring was established to use MostCare. After positioning, four electrodes were placed on patients skin. Stoke volume index along with cardiac index (CI), heart rate, systolic and diastolic pressure were recorded before surgical incision (t0), after pericardiotomy (t1), weaning from CPB and aortic catheter withdrawed (t2), after skin closure (t3). Pearson correlations were calculated and agreement were compared by Bland-Altman analysis between the two devices. Meanwhile, by observing and analyzing factors that affect correct readings of the two devices, to eliminate factors related with nursing. Adverse events related to the using of the two devices were recorded.Results No adverse events showed up in surgery, the process of surgery were not affected. Specialized intraoperative nursing ensure the two devices providing reliable and continuous readings. A good linear correlation was found between Icon-and MostCare-SVI at each time point (r=0.84,0.66,0.87,0.81;P<0.001 for all). At Bland-Altman analysis the mean difference (ml/m2), 95% limits of agreement (ml/m2), and percentage error between the two devices at four time points were (-0.4,-8.4-7.6,27.2%), (-7.2,-17.4-3.4,42.6%), (-1.8,-7.9-4.6,29.7%), (-1.2,-6.1-4.0,25.6%), respectively.Conclusions In children undergoing open heart surgery, except the period from thoractomy to pre-cardiopulmonary bypass, Icon-and MostCare-SVI readings were interchangeable. Sufficient preoperative preparation, specialized intraoperative nursing can ensure the two devices function safely and correctively.

Objective? To use near-infrared spectroscopy (NIRS) to monitor the effects of peripheral artery catheterization on limb muscle oxygen saturation (SmO2) in infants and children. Methods? Totally 60 children aged under 36 months receiving selective operation in Children's Hospital, Capital Institute of Pediatrics between March and September 2017 were selected. NIRS was used to monitor and record the SmO2 of muscle of thenar or gastrocnemius muscle on the limbs of both sides before and after trachea cannula and artery cannulation under general anesthesia. The original SmO2 values of the limbs with or without cannulation were taken as controls to observe the changes of SmO2 of the limbs with cannulation after cannulation. Results? There was no statistically significant difference in original SmO2 values of the limbs with or without cannulation before cannulation (P＞0.05). The SmO2 values of the limbs with cannulation dropped after cannulation, which were lower than the values of the limbs without cannulation (P＜0.001). The decreasing range of SmO2 values of the children aged under 6 months were larger than those of the children aged between 6 and 12 months and between 12 and 36 months; and the decreasing range of SmO2 values of the children weighing under 10 kg were larger than those of the children weighing between 10 and 20 kg (P＜ 0.001). Conclusions? NIRS can accurately reflect the changes of SmO2 after artery cannulation in real time. NIRS used in monitoring helps to assess limb perfusion more precisely. Monitoring should be enhanced for limb perfusion in children aged under 6 months and weighing under 10 kg.

Objective To evaluate masticatory performance and life quality of children with ectodermal dysplasia(ED) after prosthetic rehabilitation.Methods Six children with ED received denture restoration and 18 healthy children were involved in this study.The surface electromyography(EMG) of masseter(MM) and anterior temporalis(TA) during clenching and chewing movement were recorded.The EMG amplitude,area,asymmetry index of total and activity index of MM/TA were compared at each stage.The masticatory efficiency was measured with spectrophotometer.The life quality was assessed using visual analogue scale questionnaire.Results The EMG amplitude of MM and TA during chewing in ED Group were 41.7％ and 45.6％ of the control group respectively,the area were 35.9％ and 36.0％ respectively.Significant difference in asymmetry index of total during clenching was observed between the two groups (P＜0.05) but not during chewing(P＞0.05).The differences of activity index of MM/TA during clenching and chewing between the two groups were not detected(P＞0.05).The masticatory efficiency of ED group was 67.2％ of the control group.The score of chewing function in children with ED after prosthetic rehabilitation was three times higher than before,and no difference was present between the two groups (P＞0.05).Conclusions Early prosthetic rehabilitation can significantly improve the masticatory performance and life quality of children with ED.

Objective:To analyze the clinical and radiographic effectiveness of a calcium silicate-based bioactive ceramic iRoot BP Plus? pulpotomy of immature permanent teeth with complicated crown fracture and to evaluate the factors influencing its long-term success rate.Methods:The digital medical records of patients under 13 years old who had undergone iRoot BP Plus? pulpotomy in the Department of Oral Emergency or the First Clinical Division,Peking University School and Hospital of Stomatology from March 2017 to September 2022 due to complicated crown fracture of anterior teeth,and had taken at least one post-operation apical radiograph were reviewed.The clinical and radiographic information at the initial examination and follow-up period were obtained,including crown color,mobility,percussion,cold test(partial pulpotomy teeth),dental restoration,fistula,swelling or inflammation of the gingival tissue,the formation of apical foramen,pathologic radiolucency and calcification of pulp chamber or root canal obliteration.Data were tested by Fisher exact test and a multiple comparison.Results:In the study,64 patients including 37 males(57.8％)and 27 females(42.2％)with a mean age of 9.1 years were finally enrolled.The total number of permanent teeth that received pulpotomy was 75,and the average follow-up time was 19.3 months.The success rate was 93.1％with the time interval between dental injury and treatment in 24 h,while the success rate dropped to 88.2％with the time intervals beyond 24 h.The time intervals did not significantly affect the pulp survival rate(P=0.61)after pulpotomy(partial or co-ronal).The success rate 6 months after pulpotomy was 96.0％,and one-year success rate was 94.7％.A total of 23 cases were reviewed for more than 2 years after pulpotomy,and 6 cases failed.The mobility had no significant effect on the success rate(P=0.28).Pulp chamber calcification and pulp canal obli-teration were not observed in all the post-operative radiographs.Conclusion:The one year clinical and radiographic success rates obtained in this study indicate that iRoot BP Plus? is an appropriate pulp cap-ping material option for pulpotomy treatment of complicated crown fracture in immature permanent teeth without displacement injuries.This technique has broad promotional value.