Object To study the antitumor action of KANGXIANLING (KXL), a traditional herbal preparation and its mechanism of action at molecular pharmacological level. Methods Effect of KXL containing serum on cell periferation and protein expression of P 21 WAF1/CIP1 of human hepatic cancer HepG 2 were studied by serologic pharmacologic tests. Results KXL containing serum at concentrations of 18, 9, 3 g/kg inhibited proliferation of ex vivo cultured HepG 2 cells by (63.09?5.08)%,(55.09?4.09)%, and (48.08?4.08)% respectively. Their cytotoxicities were (48.06?4.08)%, (38.09?5.01)%, and ( 25.00? 4.13)% respectively, which were all higher than that of blank serum without KXL (P

Objective Effect of spirulina's complex preparation(SACP) on morphologies and protein expression of HSP70 in myocardium, skeletal muscle and liver tissue were investigated in the mice during 4-week prolonged exhaustive exercise . Methods Male mice were divided into 5 groups as follow: N(control), M(exercise with moderate intensity), MS(exercise with moderate intensity plus spirulina supplementation), F(exercise with increasing intensity) and FS(exercise with increasing intensity plus spirulina supplementation). Light microscope and electron microscope were used to observe the effect of SACP on morphologies in myocardium, skeletal muscle and liver tissue in mice in group F and group FS. HSP70 protein expression was detected by immunohistochemical method. Results Under the light microscope, edema, degeneration and necroses in myocardium, skeletal muscle and liver tissues were found in the mice of group F, and fragmentation of muscle fiber, cell swelling, mitochondrial swelling and nucleoplasm solution in myocardium, skeletal muscle were found under the electron microscope in the mice of group F. The aforementioned lesions of myocardium, skeletal muscle and liver tissue in the mice of group FS were obviously alleiated. HSP70 protein expression in myocardium, skeletal muscle cells and liver cells in swimming mice was significantly higher than that in control (P0.05). Conclusions SACP had protecting effect against the microtrauma in myocardium, skeletal muscle and liver tissues caused by prolonged exhaustive exercise, and the effect was probably related to the improvement of the protein expression of HSP70 affected by SACP supplementation.

The experiments were carried out in mice to investigate the effects of acupuncturing Tsusanli (AT) on physical work capacity and neuroendocrino-immunological system. Mice were divided into four groups. The general health condition of the animals during the experimental period, the swimming duration, the content of hemoglobin and T, C, T/C, C 3, C 4 of alexin in the serum, the coefficients of spleen and thymus were observed. Results showed that AT could effectively improve the general health condition of the mice and strengthen the mice endurance as manifested by the prolonged swimming time,the increased content of hemoglobin and T, T/C, C 3, C 4 of alexin in the serum, and the higher coefficient of spleen and thymus gland. These indicated that AT was effective in elimination of fatigue and improving the endurance performance,showing the possibility of its appllication in the field of sports medicine.

Objective To study the immunophenotypic feature of CD+2 adult B cell acute lymphoblastic leukemia (CD+2 B-ALL) and provide evidences for the diagnosis, therapy and prognosis. Methods The immunophenotypes of 18 cases of adult CD+2 B-ALL and 68 cases of adult CD-2 B cell acute lymphoblastic leukemia(CD-2 B-ALL) were assayed by a panel of monoclonal antibodies (McAbs) with FACS. Results The age of CD+2 B-ALL adults was younger compared to that of CD-2 B-ALL. The patients in the CD; group were similar to CD-2 group for the expression of most of the cell surface antigens assayed. However, it was notable that expression frequencies of CD10 [(73.78±26.67) %] in CD+2 B-ALL was higher than those in CD; B-ALL [(52.84±35.25) %] (t = 2.35, P<0.05) and CD33 [(15.46±27.41) %] were significantly lower than that of CD2 BALL [(31.15+27.72) %] (t = 2.16, P<0.05). Both groups showed high positive expression for CD34 72.2 % (13/ 18) and 80.9 % (55/68), respectively (χ2 = 0.64, P >0.05). The CD20 expression in CD+2 B-ALL was lower than that in CD-2 B-ALL significantly (χ2 = 11.38, P <0.05). The myeloid antigen (CD13 or CD33) expression in CD+2 B-ALL 44.4 % (8/18) was lower than that in CD-2 B-ALL 72.1 %(49/68) (χ2 = 4.86, P <0.05). Conclusion Patients in the CD+2 B-ALL were similar to CD-2 B-ALL for expression of most of the cell surface antigens assayed. CD+2 B-ALL showed low expression frequency of myeloid antigens (CD13, CD33) and CD20 antigens than CD-2 B-ALL. These results suggested that those with the CD+2 B-ALL immunophenotype generally presented with favorable prognostic features and usually could achieve good outcomes after treatment.

OBJECTIVETo study the clinical, biological features and prognosis of acute biphenotypic leukemia (BAL) in the adults.

METHODSBone marrow specimens of 63 BAL patients were evaluated to prove the diagnosis and the classification by morphologic, cytochemical, immunologic and cytogenetic (MIC) examinations. These patients were treated with protocols suitable for acute myeloid leukemia (AML), or acute lymphoblastic leukemia (ALL), or both.

RESULTSNo significant difference in clinical features was observed between BAL, AML or ALL. Morphologically, the subtypes of M(5), M(1) and M(2) were predominant in AML, as L(2) and L(1) were in ALL. Immunologically, coexpression of myeloid and B lineage associated antigens was predominant and CD(34) was hyperexpressed in BAL, which suggested that BAL might originate from malignant transformation of earlier hematopoietic cells. Cytogenetically, Ph chromosome was observed in 25.5% (13/51) of BAL patients. Prognostically, both the treatment response and the overall survival of BAL patients were poor.

CONCLUSIONPatients with BAL have unique clinical, biological and prognostic features.

Acute Disease ; Adolescent ; Adult ; Aged ; Aged, 80 and over ; Child ; Child, Preschool ; Cytogenetics ; Female ; Humans ; Leukemia, Myeloid ; drug therapy ; genetics ; immunology ; physiopathology ; Male ; Middle Aged ; Precursor Cell Lymphoblastic Leukemia-Lymphoma ; drug therapy ; genetics ; immunology ; physiopathology

The petroleum ether fraction of ethanol extract of Acorus tatarinowii were separated by column chromatography and recrystallization to afford seven compounds. On the spectroscopic analysis, they were identified as 1-hydroxy-7(11),9-guaiadien-8-one (1), calamenone(2), cis-asarone(3), chrysophanol (4), physcion (5), emodin (6), (+)-galbacin (7). Compound 1 is a new compound. Compounds 4-7 were isolated from this plant for the first time.
Acorus ; chemistry ; Ethanol ; Ether ; Plant Extracts ; analysis

OBJECTIVETo study the chemical constituents in fruit of Alpinia oxyphylla and their cytotoxicities on cancer cell lines.

METHODCompounds were isolated and purified by various column chromatographic methods. Their structures were determined by physico-chemical properties and spectral analyses. Compound cytotoxicity was assessed by the sulforhodamine B (SRB) assay.

RESULTEight compounds were obtained from Me2CO-H2O (70%) extract of the fruit of A. oxyphylla and their structures were identified as: (9E)-humulene-2, 3; 6, 7-diepoxide (1), 3(12), 7(13), 9(E)-humulatriene-2, 6-diol (2), (-)-oplopanone (3), yakuchinone A (4), yakuchinone B (5), tectochrysin (6), isovanillin (7), (2E, 4E)-6-hydroxy-2, 6-dimethylhepta-2, 4-dienal (8), and the cytotoxicities of compounds 1, 3-5 on cancer cell lines, A549, HT-29 and SGC-7901, were also investigated.

CONCLUSIONCompounds 1-3, 7, 8 were isolated for the first time from this genus and compounds 1, 3-5 exhibited no cytotoxicity against three cancer cell lines at a concentration of 10 mg x L(-1).

Alpinia ; chemistry ; Benzaldehydes ; chemistry ; isolation & purification ; pharmacology ; Cell Line, Tumor ; Cell Survival ; drug effects ; Diarylheptanoids ; chemistry ; isolation & purification ; pharmacology ; Drugs, Chinese Herbal ; chemistry ; isolation & purification ; pharmacology ; Flavonoids ; chemistry ; isolation & purification ; pharmacology ; Fruit ; chemistry ; Guaiacol ; analogs & derivatives ; chemistry ; isolation & purification ; pharmacology ; HT29 Cells ; Humans