OBJECTIVE: To compare the trauma of neck dissection on the human body between two-striae incision and traditional "L" shaped incision by serum trauma cytokines. METHODS: Patients with differentiated thyroid carcinoma hospitized from December 2008 to July 2011 were divided into 2 groups according to their own will. The first group 26 patients) had two-striae incision and the second group 32 patients) had traditional "L" shaped incision. The serum level of interleukin(IL)-2, IL-6 and C-reactive protein (CRP) in all patients were examined 1 day before and 1, 3 and 5 days after the surgery. RESULTS: No statistical significance was found between the 2 groups, although level of IL-2 decreased 1 day after the surgery, but recovered to normal 3 days later. The level of IL-6 in both groups increased 1 day after the surgery, began to decrease 3 days after the surgery, and recovered to normal 5 days after the surgery. The level of CRP suggested statistical significance (P<0.05), which increased obviously 1, 3 and 5 days after the surgery. No statistical difference was found before or after the surgery between the 2 groups (P>0.05). After follow-up for 8-40 months, no local recurrence or lymph node metastasis was found. CONCLUSION Compared with the traditional "L" shaped incision, two-striae incision in neck dissection does not increase the serum level of trauma cytokines and trauma to human body after the surgery. Two-striae incision is an ideal surgical approach to differentiated thyroid carcinoma.
Adult ; C-Reactive Protein ; metabolism ; Carcinoma, Papillary ; blood ; surgery ; Cytokines ; blood ; Female ; Humans ; Interleukin-2 ; blood ; Interleukin-6 ; blood ; Male ; Middle Aged ; Neck Dissection ; methods ; Thyroid Neoplasms ; blood ; surgery

Objective The purpose of this study was to investigate the bacterial communities of biting midges and ticks collected from three sites in the Poyang Lake area,namely,Qunlu Practice Base,Peach Blossom Garden,and Huangtong Animal Husbandry,and whether vectors carry any bacterial pathogens that may cause diseases to humans,to provide scientific basis for prospective pathogen discovery and disease prevention and control. Methods Using a metataxonomics approach in concert with full-length 16S rRNA gene sequencing and operational phylogenetic unit(OPU)analysis,we characterized the species-level microbial community structure of two important vector species,biting midges and ticks,including 33 arthropod samples comprising 3,885 individuals,collected around Poyang Lake. Results A total of 662 OPUs were classified in biting midges,including 195 known species and 373 potentially new species,and 618 OPUs were classified in ticks,including 217 known species and 326 potentially new species.Surprisingly,OPUs with potentially pathogenicity were detected in both arthropod vectors,with 66 known species of biting midges reported to carry potential pathogens,including Asaia lannensis and Rickettsia bellii,compared to 50 in ticks,such as Acinetobacter lwoffii and Staphylococcus sciuri.We found that Proteobacteria was the most dominant group in both midges and ticks.Furthermore,the outcomes demonstrated that the microbiota of midges and ticks tend to be governed by a few highly abundant bacteria.Pantoea sp7 was predominant in biting midges,while Coxiella sp1 was enriched in ticks.Meanwhile,Coxiella spp.,which may be essential for the survival of Haemaphysalis longicornis Neumann,were detected in all tick samples.The identification of dominant species and pathogens of biting midges and ticks in this study serves to broaden our knowledge associated to microbes of arthropod vectors. Conclusion Biting midges and ticks carry large numbers of known and potentially novel bacteria,and carry a wide range of potentially pathogenic bacteria,which may pose a risk of infection to humans and animals.The microbial communities of midges and ticks tend to be dominated by a few highly abundant bacteria.