Objectives　To evaluate prospectively the bone markers of serum osteocalcin (BGP),bone-specific alkaline phosphatase (BAP),C-terminal peptide of type I collagen (CICP),urinary pyridinoline (PYD),urinary calcium to creatinine value (uCa/Cr),and hydroxyprolin to creatinine ratio (uHOP/Cr),and to assess their responsiveness to subtle changes of bone metabolism,and furthermore to compare which of the bone markers are more sensitive to estrogen shortage caused by menopause.Methods　Markers of above bone formation and bone resorption were measured in 146 women classified into three groups: group I: 51 normal premenopausal women,group II:42 postmenopausal women,and group III: 53 bilateral ovariectimized women.Correlation analysis was made between these indexes and bone mineral density (BMD).Results　The mean values of the four bone markers of BGP,BAP,CICP,PYD in group II and III were significantly higher than those in group I (P<0.05).All correlations between the biochemical markers of BGP,BAP,CICP and PYD for bone turnover were highly significant and exhibited a significant connection to BMD.The best correlation was found between BGP and PYD,BGP and BAP.However,the levels of Ca/Cr and HOP/Cr made no difference in the three groups,and there was no correlation with any other indexes.Conclusion　In postmenopausal women (natural menopause and surgical menopause) suffering from severe E2 dificiency,BGP,BAP,CICP and PYD are significantly increased,indicating a clear correlation between E2 diffciency and these markers of bone turnover,so they are clinically useful in the assessment of bone turnover during changes of the estrogen status that modify the metabolic activity of the skeleton.But the traditional markers of Ca/Cr and HOP/Cr have limitations in gynecological practice because of lack of sensitivity and specificity.

0 05) While patients′ satisfactory rate was significantly higher in electrical stimulation group than in medical group( P

Objective To investigate the therapeutical effect of Resina draconis. on endometriosis rat model. Methods Totally, 60 adult rats were selected and divided into blank control group( n=10) and blood stasis endometriosis model group( n=50 ) . The blood stasis endometriosis model group was randomly divided into 5 groups after modeling: the Resina draconis. high-, medium-, and low-dose group, Guizhi Fuling group and model control group, 10 rats in each group.The Resina draconis. at 0.50, 0.25 and 0.12 g.kg-1 .d-1 was administered to the rats in the Resina draconis. high-, medium-, and low-dose groups, Guizhi Fuling capsule at 0. 25 g . kg-1 . d-1 to those in Guizhi Fuling group, and the same volume of 0. 5% sodium carboxyl methyl cellulose solution to those in model control group and blank control group. All treatments lasted for 3 weeks.At the end of the treatment, the rats were sacrificed;the volume and weight of ectopic tissue were measured. The blood viscosity was detected with blood rheometer.The concentration of estradiol and progesterone was detected using enzyme-linked immune sorbent assay.The matrix metalloprotein-2 expression in ectopic tissues was determined by RT-PCR. Results High, medium and low dose Resina draconis. significantly reduced the volume and weight of the ectopic tissue, and inhibited MMP-2 expression in ectopic tissues.High-, medium-dose Resina draconis. significantly reduced plasma cutting middle and low shear viscosity of the rats.But the Resina draconis. had no obvious effect on serum estradiol and progesterone concentration. Conclusion Resina draconis. reduces blood viscosity and inhibits MMP-2 expression, which can be used for the treatment of blood stasis type of endometriosis.

Objective To study the quality control methods for dragon's blood spraying film agent. Methods The pH value and viscosity of dragon's blood spraying film agent were detected. Drug dispersed homogeneous degree and particle sizes were determined with Nano Particle Size Analyzer and microscope. Content of Loureirin B was measured by Ultra Performance Liquid-Chromtography (UPLC). UPLC was performed on Waters C18 column (2. 1 mm×100 mm,1. 7 μm), the wavelength was 280 nm, the column temperature was 40 ℃ , and the mobile phase was 0. 1% formic acid aqueous solution and acetonitrile, and the flow rate was 0. 8 mL·min-1 . Results The pH value and viscosity of dragon's blood spraying film agent were stable, drug dispersion was homogeneous, and particle size of the drug was tiny. The concentration of Loureirin B had a good linear relationship in the range of 15. 51-77. 54 μg. Conclusion This method can be accurately controlled, has good stability and repeatability, and can fully control quality of dragon's blood spraying film agent.

Humans ; Magnetic Resonance Imaging ; trends ; Molecular Imaging ; trends ; Plaque, Atherosclerotic ; diagnosis

Objective To study the film￣forming property of film￣forming materials in dragon blood ethanol solution, and provide the reference for screening of film￣forming materials for dragon blood plastics. Methods PVA124,PVA1788, PVP￣K30, EC, MC, PVB were chosen to study the film￣forming property of film￣forming materials based on solubility, formation time, and adhesion. Results PVA1788 and PVP￣K30 mixed in dragon blood ethanol solution in proportion of 4:1 showed good film￣forming property, and the membrane was smooth, uniform and easy to tear off.However, PVA124 and PVP￣K30 mixed in proportion of 4:1 precipitated in dragon blood ethanol solution.EC, MC and PVB were not easy to tear off the membrane. Conclusion PVA1788 and PVP￣K30 mixed in proportion of 4:1 and added with propylene glycol is the optimal choice of film￣forming material for dragon blood spray film.

Objective To explore the preventive effects of different extracts from Aspidopterys obcordata on renal tubu-lar epithelial cells injury induced by sodium oxalate in vitro, and initially identify the effective part for treating urolithiasis. Methods The injury model of HK-2 cells induced by sodium oxalate was established to screen the active parts of Aspidopterys obcordata by testing the protective effects of different polarity extracts on HK-2 injury cells through MTT method. Results Dif-ferent extracts from Aspidopterys obcordata improved the activity of HK-2 injury cells, which were elevated to 86.17% and 95.42%by 0. 5 mg?mL-1 and 1 mg?mL-1 aqueous extract, respectively. And the activity reached to 93. 59% and 84. 77% by 0.5 mg?mL-1 and 1 mg?mL-1 50% alcohol extracts, reached above 81.56% by 95% alcohol extracts,all of which showed sig-nificant difference compared with the model group. The HK-2 cells viability were elevated to 82. 53% and 91. 58% by 0.5 mg?mL-1 and 1 mg?mL-1 95% alcohol extracted ethyl acetate parts,and increased to 77.24% and 87.22% by 0.5 mg?mL-1and 1 mg?mL-1 of 95% alcohol isolated n-butanol extracts, approached to 95.46% and 81.36% by 0.5 mg?mL-1 and 1 mg?mL-1 water extracts, all of which showed significant difference compared with the model control group. Conclusion The aqueous extracts and alcohol extracts from Aspidopterys obcordata have obvious preventive effects on HK-2 cells injury, among which the ethyl acetate extracts, n-butanol extracts and water extracts present the remarkable effects, which are supposed to be the active parts for inhibiting calcium oxalate stone formation in vitro.

Objective To investigate the relationship of HSP27 expression with treatment response ,remission rate ,prognosis in multiple myeloma(MM) patients .Methods Eighty‐six patients with MM first diagnosis and 27 relapse cases were collected re‐spectively .Control was defined as non‐hematological malignance .The MM patients divided into first diagnosis ,relapse conventional chemotherapy group and bortezomib group .HSP27 expression and protein level were measured on pre‐and post 1 ,2 and 3 treatment circles .Results The tendency of HSP expression and protein level:relapse‐MM patients> first‐diagnosis MM patients> control . HSP27 expression and level in Ⅲ phase was higher than those in Ⅱ phase for first‐diagnosis MM patients .Bortizomib‐containing chemotherapy protocol showed more effectively decrease on HSP27 than chemotherapy treatment only .At post‐1 treatment circle , HSP27<1 showed higher survival rate than HSP27>1 and delayed the relapse time .Conclusion BMMNC‐HSP27 expression and protein level is associated with staging ,treatment response and prognosis in MM patients .

OBJECTIVETo analyze the genetic relationship of 9 Marsdenia species from Yunnan, especially the traditional Dai medicine "Dai Bai Jie" (M. auricularis).

METHODApplying the inter-simple sequence repeats (ISSR) markers technology.

RESULTTwenty-five primers were screened out of 60 ISSR random primers and produced 391 bands totally, every primer produce 8-21 bands and the mean number was 15.6. The range of the GS (genetic similarity) value was 0.6675-0.8210. In 9 Marsdenia species, M. auricularis is a relative of M. tenacissima. M. balansae and M. officinalis have the closest genetic relationship.

CONCLUSIONIt is supported by ISSR that the M. auricularis which is sib species of M. tenacissima, and the folk medicine of Marsdenia are worthy deep investigation and study.

China ; Genetic Markers ; genetics ; Marsdenia ; classification ; genetics ; Medicine, Chinese Traditional ; Microsatellite Repeats ; genetics ; Phylogeny ; Plants, Medicinal ; classification ; genetics ; Polymerase Chain Reaction

This paper is based on the theory of"internal diseases will be presented externally"in Traditional Chinese Medicine, combined with the idea of syndrome differentiation and grasping the main symptoms, and proposes the concept of"virtual special disease". It is extracted from ancient books and expert experience, and it is believed that it is widespread and can be tested by clinical practice. The application of virtual syndrome to diagnosis can achieve the purpose of simplifying the complexity TCM syndrome differentiation. On the one hand, it provides a new exploration for enriching the TCM syndrome differentiation system; on the other hand, it provides new ideas for supplementing the biological research of syndromes and developing the diagnosis of integrated traditional Chinese and Western medicine.