1.Relationship between of fragility erythrocyte membrane and false increase of white blood cell count
Mei WANG ; Tieying YI ; Hongchu CHEN ;
Journal of Chongqing Medical University 2003;0(05):-
Objective:To explore the relationship between false increase of white blood cell count and fragility of erythrocyte membrane in automatic whole blood count. Methods: The osmosis and incubation osmosis fragility of erythrocyte membrane in controls 1 (Group 1, n =30)and white blood cell count false increase (Group 2, n =30) were detected by the operation rule of the group of clinical examination. Results: Compared with the results of Group 1 frogility of erythrocy to membrane in infectious hepatitis,livercirrhosis and liver cancer were reduced( P
4.Establishment of Teaching Files in Medicine Curriculum Construction
Ruilin CHEN ; Miqing XU ; Yi TAO ; Mei CAO ; Ziguan YE
Chinese Journal of Medical Education Research 2002;0(01):-
The standard administration and the management of the teaching files have been built firstly in the teaching and research apartment of medicine.A variety of databases to achieve files informatics and a series of teaching resources have been reinforced for recent years.Informatics technology,curriculum construction and teaching methods have been conformed to take full advantage of the teaching files.
5.Modified nasolacrimal duct stenting
Min TIAN ; Mei JIN ; Huanjun CHEN ; Yi LI
Journal of Interventional Radiology 2001;0(06):-
Objective Traditional nasolacrimal duct stenting possesses some shortcoming including difficulty of pulling ball head guide wire from the nasal cavity with turbinate hypertrophy and nasal septal deviation. The new method of nose-oral tube track establishment can overcome the forementioned and increase the successful rate. Methods 5 F catheter and arterial sheath were modified to be nasolacrimal duct stent delivery device respectively. Antegrade dacryocystography was taken firstly to display the obstructed site and followed by the modified protocol of inserting the guide wire through nasolacrimal duct and nasal cavity, and establishing the stent delivery track for retrograde stent placement. Results 5 epiphora patients with failure implantation by traditional method were all succeeded through the modified stenting(100%). During 6-mouth follow-up, no serious complications and reocclusion occurred. Conclusion The establishment of eye-nose-mouth-nose of external nasal guide wire track can improve the successful rate of nasolacrimal duct stenting.
6.EFFECTS OF DAIDZEIN ON THE ENZYMES OF BONE METABOLISM AND BONE REBUILDING IN OVARIECTOMIZED RATS
Mei GAO ; Bangquan JIN ; Yi ZHANG ; Chen LIU
Acta Nutrimenta Sinica 1956;0(02):-
Obejective To study the effect of daidzein(Daid) on the enzymes of bone metabolism and bone rebuilding in ovariectomized rats.Method Sixty 3-mon female SD rats were divided into 6 groups:Sham,OVX,OVX-D1~D4 groups.Every day the rats in Sham and OVX groups were given distilled water,and the rats in OVX-D1,OVX-D2,OVX-D3,OVX-D4 groups were given 25,50,75,100 mg/kg bw Daid respectively.After 6 mon,all of rats were killed,and the blood,liver and femur were collected.The content of calcium and phosphorus in serum and the enzymes of bone metabolism were measured.Results The content of calcium,phosphorus and ATPase in serum was increased,but the activity of serum alkaline phosphatase(ALP) and tartrate-resistant acid phosphatase(TRAP-5b) was decreased dose-dependently in OVX-D groups compared with OVX group.Conclusion The expression of bone metabolism-associated enzymes could be inhibited,which resulted in inhibition of bone formation and absorbsion in ovariectomized rats.The new balance between bone formation and absorption could be rebuilt by Daid,through the mechanism of decreasing rate of bone conversion.
7.Rapid diagnosis of trisomy 21 syndrome by denaturing high-performance liquid chromatography
Xiaoli HUANG ; Duanrong MEI ; Yi HUANG ; Yanan WU ; Hao CHEN
International Journal of Laboratory Medicine 2014;(21):2879-2880
Objective To detect the double DNA of trisomy 21 syndrome patients by denaturing high-performance liquid chro-matography(DHPLC) in order to rapidly diagnose trisomy 21 syndrome .Methods To amplify DNA fragments of three short tan-dem repeats of D21S111 ,D21S1411 and D21S1412 using corresponding primers .Then DHPLC was introduced to analyze the DNA fragments in the temperature of 50 ℃ .Results DHPLC elution profiles of D21S111 ,D21S1411 and D21S1412 of normal control showed one peak or two peaks with the same altitude .However DHPLC elution profiles of 21 trisomy syndrome patients showed two peaks of different altitudes ,which one′s altitude was twice than another .Conclusion DHPLC is a sensitive ,convenient ,auto-matic and highly-efficient method to diagnose trisomy 21 syndrome and can be widely used in the clinic diagnosis .
8.Genetic Engineering Progresses in Plant Resistance to Salt Stress
Yi-Min HE ; Hong-Juan NIAN ; Li-Mei CHEN ;
China Biotechnology 2006;0(03):-
Salinity is the main limitation factor for plant growth and crop production.Many approaches to enhance plant resistance to salinity by genetic engineering have been developed.Over-expressions of salt-tolerance related genes encoding proteins involved in signal transduction pathways,ion channels and compatible solutes synthesis for the stabilization of biological structures under salinity stress are the most often used strategies.The recent progresses in genetic engineering to improve salt tolerance in plants and the possible problems in researches was reviewed.
9.The impact of health management strategy for the quality of life of outpatients with functional dyspepsia
Xiya WANG ; Mei′e NIU ; Yi CHEN ; Anwei XIE ;
Chinese Journal of Practical Nursing 2016;32(12):943-947
Objective To explore the influence of application of health management strategy on quality of life in outpatients with functional dyspepsia (FD). Methods A total of 140 FD patients were divided into the intervention group and the control group by random digits table method with 70 cases in each. The patients in the intervention group received the researchers on the implementation of health management strategy, and the patients in the control group were given routine outpatient health education and telephone follow- up. Before and after the intervention in one month or three, the FD patients Quality of Life Scale (FDDQL) was used to evaluate the quality condition of the patient's life. Results Eventually 65 cases in the intervention group and 61 cases in the control group completed the research. 1 months after intervention in the intervention group, total score of life quality and score of each dimension rose faster, in addition to the dimension of pressure, total score and daily activities, anxiety, diet, sleep, feeling unwell, health, disease control dimension scores were 73.84 ± 3.45, 81.22±4.55, 71.58±6.19, 73.05±5.14, 74.74± 5.58, 74.72±3.91, 79.26±5.85, 73.75±6.32, higher than 60.59 ±5.06, 76.04±6.06, 68.41±9.78, 69.52±6.68,66.63±9.87, 64.37±6.24, 54.50±7.96, 50.76±7.54, the difference was statistically significant (t =2.160-20.590, P < 0.01 or < 0.05). Three months after intervention, the intervention group the total score of life quality and score of daily activities, anxiety, diet, sleep, feeling unwell, health, disease control, pressure dimension scores were 76.35±3.74, 85.21±5.34, 80.15±5.86, 73.21±4.99, 75.49±6.81, 75.46±3.80, 79.35± 6.39, 74.06±6.74, 70.86±7.00, significantly higher than 58.60±5.22, 72.02±6.53, 59.26±7.81, 60.90±6.36, 61.19±9.08, 62.24±6.02, 54.15±8.25, 50.13±7.67, 60.02±5.67 in the control group, the difference of two groups was statistically significant (t = 5.726-19.946, P < 0.01). Conclusions Using health management strategy intervention for FD outpatients will improve FD patient′s quality of life.
10.Reports of life experience in chronic obstructive pulmonary disease patients with exercise rehabilita-tion for over five years
Yi CHEN ; Mei′e NIU ; Hongying QIAN ; Xiuqin ZHANG ;
Chinese Journal of Practical Nursing 2016;32(10):754-757
Objective To explore life experience in chronic obstructive pulmonary disease (COPD) patients with the habit of exercise for more than 5 years,to enhance the confidence of participating in the rehabilitation in other patients, to help the medical staff pay more attention to guide the patients with COPD to take up the rehabilitation exercise. Methods The narrative research method in qualitative research was used to analyze the collected data. Results The study found that the coping approach of the contradiction between exercise and dyspnea, the ability to live together with the disease peacefully and the enthusiasm of persuing life were deserved to be learned. Conclusions Full of perseverance and persisted rehabilitation exercise may alleviate the symptoms, reduce the number of acute exacerbation of COPD and improve the quality of life for COPD patients. Medical staff should encourage COPD patients to participate in the rehabilitation in the right time with appropriate methods.