Objective:To explore the relationship between false increase of white blood cell count and fragility of erythrocyte membrane in automatic whole blood count. Methods: The osmosis and incubation osmosis fragility of erythrocyte membrane in controls 1 (Group 1, n =30)and white blood cell count false increase (Group 2, n =30) were detected by the operation rule of the group of clinical examination. Results: Compared with the results of Group 1 frogility of erythrocy to membrane in infectious hepatitis,livercirrhosis and liver cancer were reduced( P

Breast Neoplasms ; metabolism ; pathology ; Humans ; Immunohistochemistry ; methods ; standards ; Liver Neoplasms ; metabolism ; pathology ; Quality Control ; Receptor, ErbB-2 ; metabolism ; Receptors, Estrogen ; metabolism ; Reproducibility of Results

Objective To explore the influence of application of health management strategy on quality of life in outpatients with functional dyspepsia (FD). Methods A total of 140 FD patients were divided into the intervention group and the control group by random digits table method with 70 cases in each. The patients in the intervention group received the researchers on the implementation of health management strategy, and the patients in the control group were given routine outpatient health education and telephone follow- up. Before and after the intervention in one month or three, the FD patients Quality of Life Scale (FDDQL) was used to evaluate the quality condition of the patient's life. Results Eventually 65 cases in the intervention group and 61 cases in the control group completed the research. 1 months after intervention in the intervention group, total score of life quality and score of each dimension rose faster, in addition to the dimension of pressure, total score and daily activities, anxiety, diet, sleep, feeling unwell, health, disease control dimension scores were 73.84 ± 3.45, 81.22±4.55, 71.58±6.19, 73.05±5.14, 74.74± 5.58, 74.72±3.91, 79.26±5.85, 73.75±6.32, higher than 60.59 ±5.06, 76.04±6.06, 68.41±9.78, 69.52±6.68,66.63±9.87, 64.37±6.24, 54.50±7.96, 50.76±7.54, the difference was statistically significant (t =2.160-20.590, P < 0.01 or < 0.05). Three months after intervention, the intervention group the total score of life quality and score of daily activities, anxiety, diet, sleep, feeling unwell, health, disease control, pressure dimension scores were 76.35±3.74, 85.21±5.34, 80.15±5.86, 73.21±4.99, 75.49±6.81, 75.46±3.80, 79.35± 6.39, 74.06±6.74, 70.86±7.00, significantly higher than 58.60±5.22, 72.02±6.53, 59.26±7.81, 60.90±6.36, 61.19±9.08, 62.24±6.02, 54.15±8.25, 50.13±7.67, 60.02±5.67 in the control group, the difference of two groups was statistically significant (t = 5.726-19.946, P < 0.01). Conclusions Using health management strategy intervention for FD outpatients will improve FD patient′s quality of life.

Objective To explore life experience in chronic obstructive pulmonary disease (COPD) patients with the habit of exercise for more than 5 years,to enhance the confidence of participating in the rehabilitation in other patients, to help the medical staff pay more attention to guide the patients with COPD to take up the rehabilitation exercise. Methods The narrative research method in qualitative research was used to analyze the collected data. Results The study found that the coping approach of the contradiction between exercise and dyspnea, the ability to live together with the disease peacefully and the enthusiasm of persuing life were deserved to be learned. Conclusions Full of perseverance and persisted rehabilitation exercise may alleviate the symptoms, reduce the number of acute exacerbation of COPD and improve the quality of life for COPD patients. Medical staff should encourage COPD patients to participate in the rehabilitation in the right time with appropriate methods.

Streptococcus pneumoniae(S.pn) is an opportunity pathogenic bacteria,environmental factors play a key role in the pathogenicity of S.pn. It is important to study virulent gene in vivo. The S.pn suicide plasmid containing gfp reporter was constructed by fusing the genes pneumolysin and gfp,in which gfp is an excellent molecule probe in vivo. The plasmid was integrated to No.22 S.pn by homologous recombination. The recombinant S.pn was gained and evaluated in aspects of fluorescence excitation, biological character and physio-activity. The results showed it is efficient and available to report the expression of virulent genes in vivo and in vitro, which will provide a new easy method for evaluating and screening the virulent genes of S.pn in vivo.

Salinity is the main limitation factor for plant growth and crop production.Many approaches to enhance plant resistance to salinity by genetic engineering have been developed.Over-expressions of salt-tolerance related genes encoding proteins involved in signal transduction pathways,ion channels and compatible solutes synthesis for the stabilization of biological structures under salinity stress are the most often used strategies.The recent progresses in genetic engineering to improve salt tolerance in plants and the possible problems in researches was reviewed.

Objective To investigate the impact of alterations within the space of Disse micro- environment on the migration of hepatic stellate cells(HSC) during the process of liver fibrosis,and to ex plore the novel mechanism of liver fibrosis from the view of cell migration.Methods A modified in vitro Boyden chamber system to partially mimic in vivo microenvironment of Disse space of normal and liver fibrosis was employed.The effects of fibrogenetic growth factors on the migration of HSC in liver fibrosis were observed via cell migration and cell proliferation experiments.Results Enhanced platelet-derived growth factor(PDGF)-BB,transforming growth factor(TGF)-?1 and/or epithelial growth factor(EGF) in liver fibrosis resulted in an increase in migratory capacity of activated HSC.The enhanced migration of HSCs induced by PDGF-BB was partially associated with their increased proliferation,while,TGF-?1 or EGF-induced migration was proliferation independent.The elevation of basic fibroblast growth factor (bFGF) or vascular endothelial growth factor(VEGF)during liver fibrosis had no effect on the migration of HSCs.Conclusions The study provides valuable insights into the role of space of Disse microenvironment in regulating HSC migratory behavior.TGF-?1,PDGF-BB and EGF,which increased in liver fibrosis, could induce the migration of activated HSC.However,bFGF or VEGF has no such kind of effect,al- though they also increased during liver fibrosis.

Objective To investigate the nursing points of the mechanical ventilated patients after percutaneous endoscopic gatrostomy (PEG). Methods The nursing approaches of 28 mechanical ventilated patients after percutaneous endoscopic gatrostomy in our department was analyzed retrospectively. Results The nutritional status of the 28 patients was improved to different degree after enteral nutrition (EN) support by PEG. Thereby, the nutritional requirement of the mechanical ventilated patients was ensured effectively. Among these patients, 4 patients could take food by mouth after taking off ventilator and extubation and stoma tube was pulled out, 15 patients were discharged carrying with stoma tube and convalescent care was continued, 3 patients died of primary diseases. Conclusions The PEG is a simple,safe and cost-effective approach of nutritional support for EN of mechanical ventilated patients. The key point of insuring EN of severe patients is intensive nursing and to prevent and reduce the complication of PEG.

Objective To detect the double DNA of trisomy 21 syndrome patients by denaturing high-performance liquid chro-matography(DHPLC) in order to rapidly diagnose trisomy 21 syndrome .Methods To amplify DNA fragments of three short tan-dem repeats of D21S111 ,D21S1411 and D21S1412 using corresponding primers .Then DHPLC was introduced to analyze the DNA fragments in the temperature of 50 ℃ .Results DHPLC elution profiles of D21S111 ,D21S1411 and D21S1412 of normal control showed one peak or two peaks with the same altitude .However DHPLC elution profiles of 21 trisomy syndrome patients showed two peaks of different altitudes ,which one′s altitude was twice than another .Conclusion DHPLC is a sensitive ,convenient ,auto-matic and highly-efficient method to diagnose trisomy 21 syndrome and can be widely used in the clinic diagnosis .

The standard administration and the management of the teaching files have been built firstly in the teaching and research apartment of medicine.A variety of databases to achieve files informatics and a series of teaching resources have been reinforced for recent years.Informatics technology,curriculum construction and teaching methods have been conformed to take full advantage of the teaching files.