1.Repeatability and reproducibility of retinal straylight measurement using C-Quant compensation comparison method
Jin-hai, HUANG ; Shi-shi, LIN ; Qin-mei, WANG ; Bo, ZHENG ; Si-si, CHEN ; Rong-rong, GAO
Chinese Journal of Experimental Ophthalmology 2012;30(2):160-163
BackgroundIn an ideal eye there would be no light scattering at all,but the eye media is not optically ideal.Intraocular straylight causes a veil of light and reduction in the contrast of the retinal image and thus decrease the quality of vision.ObjectiveThe present study was to investigate the repeatability and reproducibility of C-Quant straylight meter( Oculus,Germany)in measuring retinal straylight of myopia and post-laser in situ keratomileusis(LASIK) corneas.MethodsThis is a prospective research.The consecutive 35 eyes of 21 myopic patients and 34 eyes of 22 patients who received LASIK were included in this trail.Retinal straylight was measured for 7 times at a period of time and analyzed quantificational to evaluate the repeatability of measurement.Thirty-eight eyes of 19 patients were measured again at 3-7 days for 3 times at a period of time to assess the reproducibility of C-Quant straylight meter.The mean standard difference (SD) and coefficient of variation (CV) were used as the credibility evaluation.This clinical study complied with Helsinki Declaration and the informed consent was obtained prior to the medical procedure.ResultsThe straylight Log(s) of 7 times measurement were all less than 0.95.The mean Log(s) were 0.92±0.12 and 0.93±0.17 respectively in myopia group and post-LASIK group,without statistically significant differences among 7 times measurement( F=0.335,P=0.812;F=1.000,P=0.409).The mean SD for the 7 times measurement was 0.07 Log units.SD and CV increased with the number of measurements.The differences of mean SD and CV between 3 times result and 6 times result were significant different (t =-2.080,P =0.045;t =-2.190,P =0.035 ).No difference was found between different time periods( t =-0.531,P=0.598 ).The difference of the results between two measurements from the same patient was 0.013.ConclusionsC-Quant is a noncontact,noninvade,rapid and convenient method for the measurement of straylight in myopia and post-LASIK eyes due to the high repeatability and reproducibility.
2.The effect of music exposure in juvenile stage on anxiety-like behavior and fear extinction in adult rat
Tuo LIANG ; Chao WANG ; Ye CAO ; Feiyifan WANG ; Si CHEN ; Mei ZHENG ; Changqi LI
Chinese Journal of Behavioral Medicine and Brain Science 2012;21(4):311-314
ObjectiveTo study the effect of music exposure in juvenile stage on anxiety-like behavior and fear extinction in adult rats.Methods4 broods of two-week-old healthy Sprague-Dawleyda rats were separated into two groups randomly.The music groups were exposed to music from 20:00 to 22:00 for 21 consecutive days.When the rats were 5 weeks old,they were caged by sex,with 8 male rats and 6 female rats in each group.When the rats were 8 weeks old,the two groups were assessed in the elevated-plus maze test,the open-field test,trained and assessed by the conditional fear training and fear extinction training.ResultsIn the elevated-plus maze test:Prior to the foot shock,the percent of time spent in the open arms in the music group (female(7.07 ± 1.14)%,male (5.12 ± 1.95 ) % ) exhibited no significant difference (P> 0.05 ) comparing with the rats in the control group (female (4.65 ± 0.86 ) %,male ( 4.86 ± 1.95 ) % ).After the foot shock,the percent of time spent in the open arms in the music group ( female ( 8.63 ± 3.35 ) %,male ( 7.79 ± 2.49 ) % ) increased comparing to the control group (female(1.48 ±0.11)%,male(4.29 ± 1.68)%) (P<0.01). In the open-field test:prior to the foot shock,the percent of time spent in the center zone of the music group ( female (6.16 ± 2.17 ) %,male (6.25 ±3.47) % ) exhibited no significant difference (P> 0.05) comparing with the rats in control group (female(5.27 ±1.95 )%,male (6.22 ± 3.13 )% ).After the foot shock,the percent of time spent in the center zone in the music group (female(8.52 ± 1.93) %,male (6.95 ± 2.46 ) % ) was larger than the control group ( female ( 3.47 ±0.93 ) %,male (4.36 ± 2.22 ) % ) (P < 0.05 ).The fear extinction training showed that the percent of freezing time exhibited no significant difference between the male and female rats of the music group and control group in the first block of the first extinction training day and the percentage of freezing time of female rats in music group was significantly lower than that of female rats in control group on the third extinction training day.The percentage of freezing time of male rats in the music group was significantly lower than that of male rats in control group on the second and third training day.ConclusionMusic exposure in juvenile stage decreases the anxiety level after foot shock,and promotes the long-term fear extinction,while has no effect on the level of fear memory.
3.Study on the Interaction of Gliotoxin with BSA
Jun-Jie CHEN ; Mei YANG ; Lian-Ru ZHANG ; Zhong-Hui ZHENG ; Si-Yang SONG ;
Microbiology 2008;0(08):-
The interaction between Gliotoxin and bovine serum albumin (BSA) was studied by the fluo-rescence, Circular Dichroism (CD) and ultraviolet visible (UV-Vis) techniques. The fluorescent experiment showed that the intrinsic fluorescence of BSA was quenched by the binding of gliotoxin in a static quenching procedure, with an association constant of 7.2?103 L/mol and in hydropobic forces. And the CD spectrum revealed that gliotoxin effected the conformation of BSA by increased the mass of ?-helix.
4.Analysis of Helicobacter pylori infection and its correlation with serum bilirubin in railway drivers
JIAO Lin lin ZHANG Lei LI Yu zheng JIN Chao mei ZHOU Rong ZHANG Si yu TIAN Mei ZHOU Fang
China Occupational Medicine 2022;49(05):577-
Objective Helicobacter pylori( ),
To investigate the infection status of HP and analyze the correlation between HP
Methods
infection and serum bilirubin in railway drivers. A total of 2 731 railway drivers in Zhengzhou locomotive depot were
-
selected as study subjects using judgment sampling method. Carbon 13 urea breath test was used to evaluate the HP infection
,
status. The metabolic indexes of HP positive group and HP negative group were compared and the relationship between HP
Results ( ) ,
infection and serum bilirubin was analyzed. The HP infection rate was 42.3% 1 156/2 731 . The older the age the
, ( ), ( P )
longer the work years and the higher the body mass index BMI the higher the HP infection rate all <0.01 . The infection
(P )
rate of HP in married people was higher than that in unmarried people <0.01 . The HP infection rate of smokers was higher
- (P ) - ,
than that of non smokers <0.01 . Compared with the HP negative group fasting blood glucose and serum levels of total
, ( - ), ( ) -
cholesterol low density lipoprotein cholesterol LDL C triglyceride and homocysteine Hcy were increased in the HP
( P ) ( - ), ,
positive group all <0.05 . The serum levels of high density lipoprotein cholesterol HDL C total bilirubin direct bilirubin
( ) - ( P )
DBIL and indirect bilirubin were lower than those in HP negative group all <0.05 . Logistic regression analysis showed that
( P )
HP infection was associated with low serum total bilirubin and low DBIL all <0.01 after adjusting for the confounding effects
, , , , , , , - , - ,
of age work years marital status smoking history fasting blood glucose total cholesterol triacylglycerol LDL C HDL C
Conclusion , , ,
and Hcy. The age work length BMI smoking and marital status are the influencing factors of HP infection in
railway drivers. HP infection is associated with low levels of total bilirubin and DBIL.
5.The Application of PBL Teaching Mode in the Theory Teaching of Microbiology
Si WANG ; Mei-Lian WANG ; Ji-Chun WANG ; Lan-Yan ZHENG ; Jun-Yan SHI ; En-Jie LUO ;
Microbiology 1992;0(04):-
PBL teaching method is a new mode of teaching which is originated from the West and implemented into China in recent years with an expectation that it would mainly develop the students’ self-learning ability,and enhance their skills of comprehensive thinking and solving actual problems.The author summarizes the practical experience of using PBL teaching methods in the theory teaching in Department of Medical Microbiology and Human Parasitology,China Medical University in the past three years,and then proved this method is very helpful to improving the students’integrated thinking by analysis of sample.At the same time the results also suggested that the students showed high enthusiasm in discussing the cases.By this way,the students showed great subjective intiative in their studies.
6.Effect of arginine vasopressin on membrane potential of dorsal root ganglion neurons in rats.
Hai-Yan HU ; Zhi-Ping SUN ; Yi-Mei ZHAO ; Jun-Qiang SI ; Yu ZHENG
Acta Physiologica Sinica 2004;56(1):107-111
The effect of arginine vasopressin (AVP) on membrane potential of neurons from dorsal root ganglion (DRG) was examined in the rat by means of intracellular recording technique. The results showed that (1) AVP induced hyperpolarization in the membrane of most DRG neurons. (2) The membrane conductance of the DRG neurons increased by 19.32% following application of AVP (p<0.05). (3) Perfusion with balance sodium solution (BSS) containing Cd(2+) (blocker of Ca(2+) channel) instead of Na+ failed to affect the AVP-induced membrane hyperpolarization of the DRG neurons (p> 0.05). After perfusion with BSS containing tetraethylammonium (TEA), however, the extent of AVP-induced hyperpolarization was reduced (p<0.05). (4) The AVP-induced hyperpolarization of the neurons was blocked by the antagonist of AVP V(1) receptors. The results demonstrate that AVP induces hyperpolarization of most DRG neurons, which might be caused by K(+) outflow mediated by AVP V(1) receptors in the membrane of the neurons.
Animals
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Arginine Vasopressin
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pharmacology
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Ganglia, Spinal
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drug effects
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physiology
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Membrane Potentials
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drug effects
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Neurons
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drug effects
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physiology
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Patch-Clamp Techniques
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Potassium Channel Blockers
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pharmacology
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Potassium Channels
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drug effects
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Rats
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Tetraethylammonium
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pharmacology
7.Trend analysis of cancer mortality in China between 1989 and 2008.
Hong-mei ZENG ; Rong-shou ZHENG ; Si-wei ZHANG ; Ping ZHAO ; Jie HE ; Wan-qing CHEN
Chinese Journal of Oncology 2012;34(7):525-531
OBJECTIVECancer is one of the leading causes of death in China. The study aimed to examine the temporal trend of cancer mortality rate during 1989-2008 in urban and rural areas of China.
METHODSThe mortality data of all cancers from 1989 to 2008 from National Cancer Registry database were sorted and checked. Age standardized mortality rates were calculated by the direct methods using the China population of 1982 and World Segi's population. Joinpoint regression was performed to obtain the annual percentage changes (APC) in mortality rates. The top ten cancer sites were calculated and analyzed. The mortality rates were compared with statistics of the United States.
RESULTSFrom 1989 to 2008, the trend of crude cancer mortality increased with an annual percentage change (APC) of 1.0%. After age standardization, the mortality rate was significantly decreased, with an APC of -1.2%. In urban areas, lung cancer was the most common cancer of death, whereas in rural areas, stomach cancer and esophageal cancer remained top cancers of death. Especially, in both urban and rural areas, the mortality of lung cancer was on increase. The mortality rates of stomach and esophageal cancers showed a decrease in urban areas. Compared with the cancer mortality rates of the United States, the Chinese cancer mortality rate in males remained highest. The decreasing trend of cancer mortality in females of China was less obvious than that of the United States.
CONCLUSIONSThe crude mortality rates of cancer in China show an increase whereas the age standardized mortality raters has declined between 1989 and 2008. Cancer is still a major public health issue threatening people's life in China. Effective intervention for cancer control and prevention is needed in the future.
China ; epidemiology ; Esophageal Neoplasms ; mortality ; Female ; Humans ; Lung Neoplasms ; mortality ; Male ; Mortality ; trends ; Neoplasms ; mortality ; Registries ; Rural Population ; Sex Factors ; Stomach Neoplasms ; mortality ; United States ; epidemiology ; Urban Population
8.Trend analysis and projection of cancer incidence in China between 1989 and 2008.
Wan-qing CHEN ; Rong-shou ZHENG ; Hong-mei ZENG ; Si-wei ZHANG ; Ping ZHAO ; Jie HE
Chinese Journal of Oncology 2012;34(7):517-524
OBJECTIVENationwide cancer incidence data were used to analyze the trends of cancer incidence in China in order to provide basic information for making cancer control strategy.
METHODSWe retrieved and re-sorted valid cancer incidence data from the National Central Cancer Registry Database over the 20 year-period 1989-2008. Crude incidence rate and age-standardized incidence rate were calculated for analysis. Annual percent changes in incidence for all cancers combined were estimated using Joinpoint software.
RESULTSThe cancer incidence rate in cancer registration areas was increased from 184.81/10(5) in 1989 to 286.69/10(5) in 2008 (from 209.33/10(5) to 307.04/10(5) in urban and from 176.10/10(5) to 269.57/10(5) in rural areas). Uptrends of crude cancer incidence were shown in both male and female in urban and rural areas over the 20 year-period. After standardized by age, overall incidence rate kept stable with 0.5% annual increase in urban and no change in rural areas. Since 2000, the cancer incidences in both sexes and areas were significantly increased. The incidence increased for most major cancers, especially lung cancer, colorectal cancer, female breast cancer and cervical cancer.
CONCLUSIONSOver the 20 year-period 1989-2008, cancer incidence of most cancers has been increasing by time. The incidences of gastric cancer, liver cancer and esophageal cancer still keep gradually increasing. The incidences of lung cancer, female breast cancer, colorectal cancer and cervical cancer are markedly going up, so that cancer prevention and control should be enhanced. Cancer registration will play an important role on cancer control in China along with the number of registries increasing and data quality improving.
Breast Neoplasms ; epidemiology ; China ; epidemiology ; Colorectal Neoplasms ; epidemiology ; Esophageal Neoplasms ; epidemiology ; Female ; Humans ; Incidence ; Liver Neoplasms ; epidemiology ; Lung Neoplasms ; epidemiology ; Male ; Neoplasms ; epidemiology ; Registries ; Rural Population ; Stomach Neoplasms ; epidemiology ; Urban Population ; Uterine Cervical Neoplasms ; epidemiology
9.Myocardial free radical metabolic changes in rats after repeated high +Gz exposure and protective effects of low-G preconditioning and tea polyphenols.
Hao ZHAN ; Zheng ZHANG ; Jiang-yang LU ; Qing-jun ZHANG ; Yi-mei XIN ; Tong LI ; Si-huang WEI
Chinese Journal of Applied Physiology 2004;20(3):249-252
AIMTo determine whether repetitive exposure to high sustained +Gz acceleration induces persisting changes in the myocardial free radical metabolism and observe the protective effects of low-G training and antioxidant tea polyphenols (TP).
METHODSThirty-two male Wistar rats were randomly divided into four groups (n=8 each): group A, restrained, was only submitted to +1 Gz for 5 min. Group B, centrifuged, was exposed to five plateaus of 30 s at +10 Gz for intermittent times, three times a week, for three weeks. Group C, low-G trained, was exposed to +2 Gz for 5 min about 1 h prior to +10 Gz stress, and group D was orally given TP at dose of 200 mg/kg about 1 h prior to +10 Gz stress. On the next day morning after last centrifuge run, the rats were decapitated and the hearts were quickly removed. Malondialdehyde (MDA) concentration and superoxide dismutase (SOD) activity were measured. Additionally, CuZn-SOD and inducible NO synthase (iNOS) enzymatic contents were examined by immunohistochemical staining and their mRNA were analyzed by semi-quantitative reverse transcription polymerase chain reaction(RT-PCR).
RESULTSCompared with group A, MDA concentration and iNOS enzymatic content in myocardial mitochondria were increased significantly (P < 0.05) in group B. Compared with group B, mitochondrial SOD activity was significantly increased in group C (P < 0.05). iNOS enzymatic content was significantly decreased in group C and D. There were no significant differences of CuZn-SOD content, CuZn-SOD and iNOS mRNA levels among the four groups.
CONCLUSIONRepeated high +Gz exposure can induce myocardial free radical metabolic disorder and mainly result in mitochondrial peroxidative injury. But low-G training and natural antioxidant TP have protective effects, and the former is better.
Acceleration ; Adaptation, Physiological ; physiology ; Animals ; Free Radicals ; metabolism ; Male ; Myocardium ; metabolism ; Polyphenols ; pharmacology ; Rats ; Rats, Wistar ; Tea ; chemistry
10.A study on the epidemic characteristics of HIV/AIDS by different route of transmission in Huzhou City
Zhong-Rong YANG ; Mei-Hua JIN ; Zheng-Quan DONG ; Si-Chao ZHANG ; Jing LI
Journal of Preventive Medicine 2016;28(12):1200-1204
Objective The aim of this study is to explore epidemic characteristics of HIV/AIDS by different route of transmission during 2011—2014 in Huzhou City,then to provide a scientific basis for the prevention and control of HIV. Methods The epidemiological case data of HIV/AIDS from 2011 to 2014 were collected in Huzhou City by China information system for disease control and prevention.Descriptive epidemiological methods were used for analysis.Results A total of 436 cases with HIV/AIDS were reported during 2011—2014 in Huzhou City,and the number of HIV/AIDS reports increased year by year.The main route of transmission include heterosexual contact,which was accounted for 56.65%.MSM transmission was accounted for 38.53%,and injection drug transmission was accounted for 3.90%.Mother to child transmission was 1 case and blood transfusion /blood products transmission were 3 cases,which accounting for 0.92%.The constituent ratio of injecting drug patients,mother to child transmission or blood transfusion /blood products patients presented declining trend (P <0.05).Patients who were infected through heterosexual contact mainly were 30 to <60 years of age (68.83%),junior middle school or below education level (80.97%),married (61.94%).Patients who were infected through MSMmainly were 15 to <45 years of age (76.19%),high school or higher education (47.62%), unmarried (51.19%),household registration of Huzhou City (52.98%).Patients who were infected through injection
drug mainly were 30 to <45 years of age (70.59%),primary school or lower education level (82.35%),unmarried (47.06%),household registration of other province (94.12%).Most cases were detected HIV positive through clinical tests,which accounting for 51.15%.Most HIV /AIDS of MSM,heterosexual transmission and inject -drugs were distributed in Wu Xing District,which accounting for 67.26%,51.42% and 64.71%,respectively.51.15% HIV /AIDS were detected by clinical test.Conclusion The reported cases of HIV /AIDS were still mainly transmitted by heterosexual contact in recent years in Huzhou City,and the constituent ratio of MSMtransmission has increased slightly.