1.Evaluation of the mass-screening of the uterine cervical cancer using Kato's Self-abrasive Device in China.
Journal of the Japanese Association of Rural Medicine 1985;34(4):808-815
Using Kato's Self-abrasive Device, mass-screening for uterine cervical cancer has been carried out for 5, 983 married women at Chicheng country of a mountainous farm village and Shijiazhuang city in Hebei province, China, and the following result has obtained:
The women who diagnosed as class III and over were 42 persons (0.70%). Cancer cases which were confirmed by the biopsy were 10 (3 at Shijiazhuang city and 7 at Chicheng country); the occurrence rate of the cervical cancer was 0.17% and no false positive was observed. The mass -screening of the uterine cervical cancer has been carried out simultaneously by both way of the self-abrasive method and the scraper method for one third of persons who will take medical advice atboth districts. The result of comparative analysis for the quality of smear and the Papanicolaouclassification by the two method was;
1. As for the self-abrasive method, mixing with erythrocytes and overlapping of cells were few compared with the scraper method, and the diagnosis was more easily made in cytological examination.
2. The rate of coincidence by both methods were 78.85% in class I, 94.14% in class II, and 100.00% in class III-V.
2.Application of foreign body cage in removal of tracheobronchial foreign bodies.
Mei-sheng LI ; Qiu-yan KONG ; Xian-jun SUN
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2007;42(7):536-536
Adolescent
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Adult
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Aged
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Bronchi
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Child
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Female
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Foreign Bodies
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surgery
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Humans
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Male
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Middle Aged
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Surgical Mesh
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Trachea
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Young Adult
3.Effect of caffeic acid, seopoletin and scutellarin on rat retinal neurons in vitro.
Yi ZHANG ; Yan-Mei SHENG ; Xian-Li MENG ; Yi LONG
China Journal of Chinese Materia Medica 2005;30(12):907-909
OBJECTIVETo observe the effect of caffeic acid, seopoletin and scutellarin on rat retinal neurons in vitro and explore neuroprotection in glaucoma of Erigeron breviscapus.
METHODThe retinal of 18 post-natal 2-3 days Sprague-Dawley rats were dissociated into cell suspension with trypsin digestion. The cell suspension was implated in 96-well culture plates covered with hyaluronic acid and laminin in each well. After culturing for 3 days, caffeic acid, seopoletin and scutellarin were added to the cultures, continue to culture 2 days. Then, the A of living cells in each well was tested by MTT colorimetric microassay. Some of the 5-day culture cells were identified by Nissel technique.
RESULTMost of the living cells were retinal neurons by Nissel identification. The number of living cells increased significantly in high concentrations of caffeic acid, seopoletin and scutellarin compared with control group (P < 0.05, P < 0.01).
CONCLUSIONcaffeic acid, seopoletin and scutellarin can all promote retinal neurons to live in vitro, with caffeic acid being most effective.
Animals ; Animals, Newborn ; Apigenin ; isolation & purification ; pharmacology ; Caffeic Acids ; isolation & purification ; pharmacology ; Cell Survival ; drug effects ; Cells, Cultured ; Dose-Response Relationship, Drug ; Erigeron ; chemistry ; Glucuronates ; isolation & purification ; pharmacology ; Neurons ; drug effects ; Neuroprotective Agents ; isolation & purification ; pharmacology ; Plants, Medicinal ; chemistry ; Rats ; Rats, Sprague-Dawley ; Retina ; cytology ; Scopoletin ; isolation & purification ; pharmacology
4.Saponin from Tupistra chinensis Bak Inhibits NF-κB Signaling in Sarcoma S-180 Cell Mouse Xenografts
Tai-Sheng YE ; Xiu-Ping WANG ; Xian-Mei ZHANG ; Man-Ling ZHANG ; Ying-Wen ZHANG
Journal of Huazhong University of Science and Technology (Medical Sciences) 2018;38(4):697-703
This study examined the effect of saponins from Tupistra chinensis Bak (STCB) on the growth of sarcoma S-180 cells in vitro and in mouse xenografts as well as the underlying mechanisms.Cell proliferation was assessed by MTT assay.Cell cycle distribution was determined by flow cytometry.Sarcoma S-180 tumor-bearing mice were treated with different doses of STCB with 10 μg/mL 5-fluorouracil (5-Fu) as a positive control.The activity of nuclear factor (NF)-κB was detected by gel mobility shift assay.The mRNA level of NF-κB was determined by real-time quantitative RT-PCR.The results showed that in vitro STCB inhibited the growth of S-18 0 cells in a concentration-dependent manner,which was accompanied by cell cycle arrest at S-phase.In vivo STCB significantly inhibited the growth of S-180 tumor mouse xenografts in a dose-dependent manner with apparent induction of cell apoptosis.Moreover,STCB inhibited the activity of NF-κB p65 and reduced the expression of NF-κB p65 mRNA in mouse xenografts.It was concluded that STCB inhibits the proliferation and cell cycle progression of S-180 cells by suppressing NF-κB signaling in mouse xenografts.Our findings suggest STCB is a promising agent for the treatment of sarcoma.
5.Adaptation of myofibrilla, MHC and metabolic enzyme of rabbit diaphragm muscle to different frequency chronic electrical stimulation.
Sui-Yang ZHANG ; Fang-Mei WANG ; Gang LIU ; Dong-Lin WANG ; Xian-Jian GUO ; Gui-Sheng QIAN
Chinese Journal of Applied Physiology 2005;21(4):367-370
AIMTo detect effect of the different frequency of chronic electrical stimulation (CES) on myofibrillar isoform, myosin heavy chain (MHC) and metabolic enzyme activities.
METHODSThe histochemical method and SDS-polyacrylamide gel electrophoresis were respectively employed.
RESULTS(1)There were a significant increase in I myo-fibrillar isoform and I MHC isoform and decrease in II B myofibrillar isoform and II B MHC isoforms in the chronic low frequency electrical stimulation (CLFES) 10 Hz and 20 Hz groups, but opposite results were found in the chronic high frequency electrical stimulation (CHFES) 50 Hz and 100 Hz groups. (2) There were a significant increase in the aerobic-oxidative enzyme activities and capacity, and a concomitant significant drop in glycolysis enzyme activities in CLFES groups, but opposite results were found in CHFES 50 Hz and 100 Hz groups.
CONCLUSIONIt was suggested that there was a significant dependent relation between chronic electrical stimulation frequency and myofibrilla isoforms, myosin heavy chain (MHC) and metabolic enzyme activities.
Adaptation, Physiological ; Animals ; Diaphragm ; enzymology ; metabolism ; physiology ; Electric Stimulation ; Muscle Contraction ; Myosin Heavy Chains ; metabolism ; Nonmuscle Myosin Type IIB ; metabolism ; Protein Isoforms ; Rabbits
6.Clinical research of vestibular autorotation test for patients with vertebrobasilar insufficiency.
Tai-sheng CHEN ; Wen-hong WANG ; Wei SONG ; Hong-hua LU ; Xian-hua ZUO ; Jin-mei ZHANG
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2006;41(10):721-725
OBJECTIVETo explore the diagnostic values of vestibular autorotation test (VAT) for patients with vertebrobasilar insufficiency (VBI).
METHODSVAT and videonystagmography ( VNG) were performed on 73 patients with VBI and 48 patients with peripheral vestibular lesions (contrast group). Parameters analyzed included Gain, phase and asymmetry of VAT, as well as the canal paresis (CP) of caloric test and results of optokinetic-pursuit tests in VNG. Positive result of the test could be defined if anyone of the parameters was abnormal.
RESULTSFor VAT test, Gain was enhanced in VBI group and was reduced in contrast group. In VBI group and contrast group, Gain enhanced showed in 47 (64.4%) cases and 5 (10.4%) cases, respectively (chi2 = 31.19, P < 0.01). Simultaneity, Gain reduced in 11 cases (15.5%) and 22 cases (45.8%), respectively (chi2 = 13.82, P < 0.01). But there was no statistics significant for results of the parameters of phase, asymmetry and integration between two groups. For VNG test, results with optokinetic-pursuit tests were more abnormal in VBI group than that in contrast group, which showed central lesions characteristics. Forty-four cases (60.3%) in VBI group and 10 cases (20.8%) in control group showed central lesions results with optokinetic-pursuit tests and visual fixation test (chi2 = 15.89, P < 0.01). Unilateral or bilateral CP showed in 33 cases (68.6%) in control group and 51 cases (69.9%) in VBI group with caloric test.
CONCLUSIONSGain of VAT is mostly enhanced in VBI group, and Gain as a main characteristic is reduced in patients with peripheral vestibular lesions. The Gain parameter is availability for assessing characteristics of vestibular lesions. Phase and asymmetry can be used to assess the vestibular function but can not indicate the characteristics of vestibular lesions.
Adolescent ; Adult ; Aged ; Child ; Female ; Humans ; Male ; Middle Aged ; Vertebrobasilar Insufficiency ; complications ; physiopathology ; Vertigo ; etiology ; physiopathology ; Vestibular Function Tests ; methods ; Young Adult
7.Effect of adriamycin on sarcoplasmic reticulum Ca2+ -ATPase activity in cardiomyocyte of rabbits
Xian-Mei HUANG ; Man-Li KANG ; Li-Zhong DU ; Zhong-Sheng YU
Journal of Zhejiang University. Medical sciences 2002;31(1):37-40
OBJECTIVE: To evaluate influences of regular-dose of adriamycin (ADR) on heart function and sarcoplasmic reticulum (SR) Ca2+ -ATPase in cardiomyocyte of rabbits. METHODS: Nine rabbits received intraveneous injection of ADR (2mg/kg) once a week for 8 weeks, the rabbits injected with saline were used as control group. Cardiac output (CO), blood pressure (BP), mean artery pressure (MAP), left ventricular systolic pressure( LVSP), left ventricular end-diastolic pressure (LVEDP), calcium in cardiomyocyte (MyoCa2+) of rabbits and SR Ca2+ -ATPase activity were examinated 3 weeks after the final injection. RESULTS: CO, LVSP and SR Ca2+ -ATPase activity were significantly decreased in ADR treated group compared with the control group. Conversely, LVEDP and MyoCa2+ were significantly increased in ADR treated rabbits. CONCLUSION: Heart function can be decreased by regular-dose of ADR in injection. Calcium overload in cardiomyocyte and decrease of SR Ca2+ -ATPase activity is important physiopathologic mechanism in ADR-induced impairment of heart.
8.A clinical study of recombinant human granulocyte colony-stimulating factor on prevention of leukopenia caused by chemotherapy
Ya-Bin SHI ; Jing-Sheng HE ; Xu-Mei SONG ; Yun-Hua WANG ; Xue-Hong ZHU ; Yu-Guo ZHAO ; Lei KOU ; Qun-Xian LU ;
Cancer Research and Clinic 2006;0(11):-
Objective To observe the preventive value of recombinant human granulocyte colony stimulating factor(rhG-CSF)in cancer patients after chemotherapy.Methods In the open study,enrolled 52 patients with previously untreated cancer and with normal bone marrow function were randomly divided into 2 matched groups,A and B group.Each patient received one cycle of chemotherapy.In the study cycle,the pa- tients received a single subcutaneous injection of rhG-CSF 150 ?g before 24 hours of chemotherapy and in control cycle the patients only received chemotherapy.Efficacy and safety parameters were monitored.Results The incidence rates of leukopenia in the 26 valuable study cycles and 26 valuable control cycles were 19.23 % and 53.85 %,There were significant lower than those of group B(P
9.Teaching research on student ability training of biomedical engineering specialty
sheng Yong LI ; mei Xiang DONG ; feng Xian ZHU ; hao Guang YU
Chinese Medical Equipment Journal 2017;38(9):151-152,158
Objective To design a student training program of biomedical engineering specialty to enhance student ability and employment rate.Methods The program design was executed from the aspects of curricula,teaching content,teaching approach,examination scheme,directed training by tutorial system as well as training integrating university and enterprise.Results The program design gifted the student with high comprehensive quality and professional skills.Conclusion Teaching approaches have to be regulated continuously according to the requirements of employing facilities.
10.Difference of gene expression profiles between HL-60/VCR and HL-60 cells detected by human genome genechip.
Bao-Xian DONG ; Xie-Qun CHEN ; Zhe WANG ; Rong LIANG ; Qing-Xian BAI ; Gao-Sheng HUANG ; Wei-Ping ZHANG ; Guang-Xun GAO ; Dong-Mei HAN
Journal of Experimental Hematology 2006;14(3):492-496
This study was aimed to detect the gene expression profile changes between human acute leukemia cell line HL-60 and VCR-resistance HL-60, and to investigate the underlying mechanisms of MDR by using genechip technology. In experiments, mRNA were harvested using TrizoL reagent from these two cell lines, through RT-PCR, the biotinylated nucleotide were incorporated into the cRNA during the in vitro transcription reaction. The high quality RNA was hybridized to the gene expression array--human genome U133A developed by Affymetrix. It was scanned by G2500A GeneArray Scanner and the acquired image was analysed by a series of softwares. The results showed that 5,507 genes were differentially expressed between human acute leukemia cell line HL-60 and VCR-resistant HL-60. Compared with HL-60, 3,100 genes were up-regulated and 2,407 genes were down-regulated in VCR-resistant cell line. These genes were involved in different cell activities such as growth regulation and signal transduction. Among the genes with remarkable differential expression between the two cell lines, 435 were up-regulated and 605 were down-regulated. It is concluded that many different kinds of genes are involved in the mechanism of MDR and there is an intricate molecular network that controls the sensitivity of leukemia cells to the chemotherapeutic agents. Genechip is an efficient tool for parallel gene expression analysis.
Drug Resistance, Neoplasm
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genetics
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Gene Expression Profiling
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Gene Expression Regulation, Neoplastic
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Genome, Human
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HL-60 Cells
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Humans
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Oligonucleotide Array Sequence Analysis
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Vincristine
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pharmacology