OBJECTIVETo compare the efficacy differences between dog-days medicinal vesiculation and regular-day medicinal vesiculation for perennial allergic rhinitis (PAR), and observe their effects on serum immune globulin E (IgE) and interleukin-4 (IL-4).

METHODSSeventy-two patients were randomly divided into a dog-days moxibustion group (34 cases) and a regular-day moxibustion group (38 cases). In the dog-days moxibustion group, medicinal vesiculation was applied on the 1st dog-day, 2nd dog-day and last dog-day in summer by lunar calendar, 3 treatments per dog-day for totally 9 times. In the regular-day moxibustion group, the moxibustion was given on the regular day for continuous 9 times. The symptom score, rhinoconjunctivitis quality of life questionnaire (RQLQ) and the level of IgE and IL-4 were compared before and after treatment in two groups; the short-term and two-year efficacy evaluation were performed too.

RESULTSThe short-term total effective rate was 88.2% (30/34) in the dog-days moxibustion group, which was not significantly different to 86.8% (33/38) in the regular-day moxibustion group (P>0.05). The long-term total effective rate was 97.1% (33/34) in the dog-days moxibustion group, which was significantly superior to 81.6% (31/38) in the regular-day moxibustion group (P<0.05). After treatment, the serum IgE, IL-4 and RQLQ were significantly reduced (all P<0.01), but the difference between two groups was not significant (all P>0.05).

CONCLUSIONMedicinal moxibustion could be taken as a regular treatment for PAR, which could be performed during the whole year, and dog-days moxibustion could be considered as an enhanced method for prevention and treatment of PAR.

Acupuncture Therapy ; Adult ; Drugs, Chinese Herbal ; administration & dosage ; Female ; Humans ; Male ; Rhinitis, Allergic, Perennial ; drug therapy ; therapy ; Treatment Outcome ; Young Adult

Female ; Humans ; Integrative Medicine ; Polycystic Ovary Syndrome ; therapy

0.05).Frequencies of P12A12 genotype and A12 allele in DN group were significantly decreased respectively,when compared with DN-0 group(for P12A12 genotype,9.1% vs 18.1%,P=0.034,OR=0.453;for A12 allele,4.5% vs 9.0%,P=0.041,OR=0.479). Conclusion The observations suggest that P12A polymorphism of PPAR?2 gene is associated with Chinese type 2 diabetic nephropathy,and A12 allele may protect the development of diabetic nephropathy in type 2 diabetic patients of Chinese.

OBJECTIVE: To investigate the genetic polymorphism of 12 STR loci in 240 Han population and evaluate application of the 12 STR loci systems in forensic cases. METHODS: 12 STR loci were amplified with Muti-loci STR PCR system, which were genotyped with electrophoresis separation on dPAGE. RESULTS: 12 STR loci system had high power of discrimination (DP) and it was suited for analysis of dated bloodstain especially. CONCLUSION The 12 STR loci systems is simple economy and practicality, which is useful in forensic identification and disputed paternity.
Alleles ; Asian People/genetics* ; China/ethnology* ; Coloring Agents ; DNA/blood* ; Electrophoresis, Polyacrylamide Gel ; Forensic Genetics/methods* ; Gene Frequency ; Genetics, Population ; Humans ; Polymerase Chain Reaction/methods* ; Polymorphism, Genetic ; Tandem Repeat Sequences/genetics*

Nasal polyp (NP) is a common chronic inflammatory disease of the nasal cavity and sinuses.Although some authors have suggested that NP is related to inflammatory factors such as interleukin (IL)-1β,IL-5,IL-8,granulocyte-macrophage colony-stimulating factor (GM-CSF),tumor necrosis factor (TNF)-α,and IL-17,the mechanisms underlying the pathogenesis and progression of NP remain obscure.This study investigated the expression and distribution of IL-17 and syndecan-1 in NP,and explored the roles of these two molecules in the pathogenesis of eosinophilic chronic rhinosinusitis with nasal polyps (Eos CRSwNP) and non-Eos CRSwNP.Real-time PCR and immunohistochemistry were used to detect the expression of IL-17 and syndecan-1 in samples [NP,unciform process (UP) from patients with CRS,and middle turbinate (MT) from healthy controls undergoing pituitary tumor surgery].The results showed that the expression levels of IL-17 and syndecan-1 were upregulated in both NP and UP tissues,but both factors were higher in NP tissues than in UP tissues.There was no significant difference in IL-17 levels between the Eos CRSwNP and non-Eos CRSwNP samples,and syndecan-1 levels were increased in the non-Eos CRSwNP tissues as compared with those in Eos CRSwNP tissues.In all of the groups,there was a close correlation between the expression of IL-17 and syndecan-1 in nasal mucosa epithelial cells,glandular epithelial cells,and inflammatory cells,suggesting that IL-17 and syndecan-1 may play a role,and interact with each other,in the pathogenesis ofnon-Eos CRSwNP.

Objective To evaluate the effects of optimal pharmacotherapy according to guideline on treating chronic heart failure(CHF)in real world clinical practice. Methods A total of 231 consecutive outpatients with reduced left ventricular ejection fraction(LVEF≤40%)and enlarged left ventricular end diastolic diameter(male ＞55 mm, female ＞60 mm)were recruited from January 2001 to June 2009. All patients were treated with optimal pharmacotherapy according to guideline recommendations and followed up to December 31,2009. Mortality, rehospitalization and changes of heart size and cardiac function at baseline and at the end of follow-up period were analyzed. Results(1)14 patients were lost during follow-up (6. 1%), and follow-up was complete in 217 patients(93.9%). 97.2% and 98.2% patients were prescribed angiotensin converting enzyme(ACE)inhibitors and β-blockers(βB). Combined of ACE inhibitors and BB use was applied in 95.3% patients. The target dose of ACE inhibitors and βB were reached in 50. 7% and 37.3% patients.(2)Lower mortality and re-hospitalization rates were observed in this cohort: all-cause morality, average annual mortality was 11.5% and 3.9% respectively. Rehospitalization rate was 27.6%.(3)Left ventricular end-diastolic diameter(LVEDD)decreased from (68.2 ±7.2)mm to(62. 2 ±9. 6)mm. LVEDD value was normal or near normal(male≤60 mm, female ≤55 mm)in 43.2% patients. LVEF improved form(29. 8 ±7. 5)% to(43. 3 ± 11.8)%, LVEF was ＞40% in 60.4% patients, LVEF was ≤ 40% but increased ≥ 10% after treatment in 22.9%patients. Conclusion Optimal pharmacotherapy according to guideline can improve prognosis of outpatients with CHF.

AIMThe interactions between diorganotin (IV) complexes of 1,3-dimethyl-4-acetyl-5-pyrazolone (HL1) and mono-nucleotides together with DNA near physiological condition were investigated.

METHODSThe mode of action of the diorganotin (IV) complexes with mononucleotides and DNA under different conditions and different times were investigated by high resolution NMR technology and UV spectra.

RESULTSThe interaction of [(L1)2SnEt2] with AMP was shown to result in significant change of chemical shift of H(8), H(2) and 31P of AMP. Hyperchromic effect of DNA could be observed due to the interaction of; [(L1)2SnEt2] with DNA, while interaction of [(L1)2SnMe2] with AMP and DNA could only cause obvious change of chemical shift of 31P and lead to hypochromic effect of DNA.

CONCLUSIONThe results indicate that [(L1)2SnEt2] can selectively bind to the N1 atom of the base and the phosphate oxygen atom of AMP and may further destroy the helical structure of DNA, while the dimethyltin (IV) compound of 1,3-dimethyl-4-acetyl-5-pyrazolone [(L1)2SnMe2] merely binds to the the phosphate oxygen atom of AMP and causes the contraction of DNA helical structure.

Antineoplastic Agents ; chemistry ; DNA ; chemistry ; Nucleotides ; chemistry ; Organotin Compounds ; chemistry ; Porphyrins ; chemistry

OBJECTIVETo explore the incidence and risk factors of campus violence in Guangzhou.

METHODS2200 college students in three universities in Guangzhou were selected by cluster sampling method and were interviewed with self-designed questionnaire about the incidence and risk factors of campus violence in 2010. The final analysis sample was 2103. Chi-square test was used to analyze the gender, grade and major distribution of campus violence. Logistic regression method was used to analyze the influencing factors of campus violence in bully and victim.

RESULTSThe incidence of campus violence in Guangzhou was 69.9% (1471/2103). In boys and girls the incidence of campus violence was 75.6% (830/1098) and 63.8% (641/1005) (χ(2) = 34.82, P < 0.05). The incidence of bully and victim of campus violence was 63.6%(1338/2103) and 55.3% (1163/2103). The incidence of bully and victim in boys was 70.9%(778/1098) and 60.0%(659/1098), and in girls was 55.7% (560/1005) and 50.1% (504/1005) (χ(2)(bully) = 51.93, χ(2)(victim) = 20.68, P < 0.01). The incidence of psychological violence was the highest (68.0%, 1430/2103), followed by sexual violence (34.2%, 719/2103), the incidence of physical violence was the lowest (16.5%, 348/2103). Results of logistic regression showed that boys (OR = 1.60), arts (OR = 1.82), with siblings (OR = 1.31), the living expenses was not enough (basic enough OR = 1.35, not enough OR = 1.54), playing the computer games (OR = 1.70), playing tricks such as sliding plate (OR = 2.03), loving violence program (general OR = 1.30, very like OR = 1.44), mother with gamble behavior (OR = 4.29), father's indulgent education style (OR = 1.60), been bullied by others before high school (OR = 1.61), dissatisfaction to the environment and rules of campus (nothing special feeling OR = 1.67, some dissatisfaction OR = 1.89), been treated badly by primary school teacher (one kind OR = 1.35, two kinds and above OR = 1.90)were the risk factors of bully. Boys (OR = 1.23), minority (OR = 1.71), with siblings (OR = 1.39), bad behavior and habit (OR = 1.32), the bad family economic conditions (general OR = 1.51, difficult OR = 1.88), mother with gamble behavior (OR = 2.33), father's indulgent education style (OR = 1.37), occasional physical punishment by mother (OR = 1.35), been bullied by others before high school (sometimes OR = 1.61, often OR = 1.85), high pressure during the study (a little high OR = 1.37, very high OR = 1.40), dissatisfaction to the environment and rules of campus (some dissatisfaction OR = 1.56, completely dissatisfaction OR = 2.04), been treated badly by primary school teacher (one kind OR = 1.70, two kinds and above OR = 2.04)were the risk factors of being victim.

CONCLUSIONThe campus violence in Guangzhou is serious, especially the psychological violence and sexual violence. And the risk factors of campus violence in bully and victim are multifold.

Adolescent ; Adult ; China ; Female ; Humans ; Incidence ; Logistic Models ; Male ; Risk Factors ; Students ; psychology ; Surveys and Questionnaires ; Universities ; Violence ; statistics & numerical data ; Young Adult

Objective To evaluate the role of cerebral state index(CSI),burst suppression (BS)and electromyograph(EMG)in monitoring coma/consciousness depth and damage degree of brain. Methods CSM was done in 50 cases with brain injury and coma to analyze its relation with physical reflection,auditory evoked potential(AEP),Glasgow coma score(GCS)and Glasgow outcome scale (GOS).Results As scale range meaning from consciousness to deep coma and to brain death,CSI 0- 100 was positively correlated with coma depth,coma score of GCS and physical reflection.CSI changes under invariable ache stimulation in combination with BS and EMG can accurately estimate prognosis and quantify changes of brain function.Conclusions The quantifiable digit of coma/consciousness depth and damage degree in brain function by CSM can attain real time judgment of dynamic evolvement course of coma and objective guide clinical therapy and assure prognosis,as will change absolutely scoring coma/ consciousness depth and prognosis under current state of artificial diversity and lacking objective evi- dences.

OBJECTIVETo evaluate the value of high-risk HPV (hrHPV) detection by Hybrid Capture II (HC2) in screening cervical intraepithelial neoplasm (CIN).

METHODSTotally 723 patients who had received a dual screening with thinprep cytologic test (TCT) and HC2 in our department were analyzed retrospectively. Among them, 350 patients received a triple examination with TCT, HC2, and colposcopic biopsy.

RESULTSAmong the 723 patients, the incidences of hrHPV infection with atypical squamous cell (ASC), low squamous intraepithelial lesion, and high squamous intraepithelial lesion were 70.7% (94/133), 88.9% (249/280), and 90.9% (90/99), respectively, significantly higher than 55.5% (117/211), the incidence of hrHPV infection with normal cytological results (P = 0.005, P < 0.001, P < 0.001, respectively). Among 350 cases who were received triple examination, the incidence of hrHPV infection with cervical intraepithelial neoplasia (CIN) 1 and CIN 2 were 88.9% (72/81) and 96.3% (52/54), significantly higher than 77.7% (153/197), the incidence of hrHPV infection with normal pathological results (P = 0.03, P = 0.002); The incidence of hrHPV infection with CIN 3 and squamous cancer were 91.7% (11/12) and 100.0% (6/6), also higher than normal cases. Among these 350 cases, the incidence of hrHPV infection with ASC was 79.3% (69/87). The incidence of CIN 2-3 with ASC and hrHPV infection was 38.0%, significantly higher than the incidence of CIN 2-3 with ASC and without hrHPV infection (5.9%) (P = 0.04).

CONCLUSIONhrHPV infection has a close relation with CIN, and the incidence of hrHPV infection increases along with the severity of CIN.

Adolescent ; Adult ; Cervical Intraepithelial Neoplasia ; virology ; Cervix Uteri ; pathology ; virology ; Female ; Human papillomavirus 16 ; isolation & purification ; Human papillomavirus 18 ; isolation & purification ; Humans ; Middle Aged ; Nucleic Acid Hybridization ; methods ; Papillomavirus Infections ; epidemiology ; virology ; Uterine Cervical Neoplasms ; virology