1.Subdural puncture in diagnosis and treatment of subdural fluid collection in infants or toddlers with purulent meningitis:report of 207 cases
Journal of Third Military Medical University 2003;0(07):-
Objective To investigate the role of subdural puncture(SDP)in the diagnosis and treatment of subdural fluid collection in young children with purulent meningitis.Methods Totally 207
3.The relationship between expression of cyclooxygenase-2,vascularendotheliai growth factor and angiogenesis in hepatoceUular carcinoma
Dong-Mei ZOU ; Qing-Xu YANG ; Tong-Tong WU ;
Chinese Journal of Primary Medicine and Pharmacy 2006;0(08):-
Objective To investigate the significance of cyclooxygenase-2 (COX-2),vascular endothelial growth factor(VEGF) protein expression and angiogenesis in hepatocellular carcinoma(HCC).Methods Tissue sec- tions from 40 HCC patients were examined immunohistochemically for protein expression of COX-2 and VEGF.Mi- crovessel density(MVD) was counted by endothelial cells immunostained by anti-CD34 antibody.Results The ex- pression of COX-2 protein in well differentiated HCC was stronger than that in moderately differentiated HCC and poorly differentiated HCC(P
4.Effects of fibrogenetic growth factors on migration of hepatic stellate cells
Chang-Qing YANG ; Yi-Zhong CHANG ; Xi-Mei CHEN ;
Chinese Journal of Digestion 2001;0(12):-
Objective To investigate the impact of alterations within the space of Disse micro- environment on the migration of hepatic stellate cells(HSC) during the process of liver fibrosis,and to ex plore the novel mechanism of liver fibrosis from the view of cell migration.Methods A modified in vitro Boyden chamber system to partially mimic in vivo microenvironment of Disse space of normal and liver fibrosis was employed.The effects of fibrogenetic growth factors on the migration of HSC in liver fibrosis were observed via cell migration and cell proliferation experiments.Results Enhanced platelet-derived growth factor(PDGF)-BB,transforming growth factor(TGF)-?1 and/or epithelial growth factor(EGF) in liver fibrosis resulted in an increase in migratory capacity of activated HSC.The enhanced migration of HSCs induced by PDGF-BB was partially associated with their increased proliferation,while,TGF-?1 or EGF-induced migration was proliferation independent.The elevation of basic fibroblast growth factor (bFGF) or vascular endothelial growth factor(VEGF)during liver fibrosis had no effect on the migration of HSCs.Conclusions The study provides valuable insights into the role of space of Disse microenvironment in regulating HSC migratory behavior.TGF-?1,PDGF-BB and EGF,which increased in liver fibrosis, could induce the migration of activated HSC.However,bFGF or VEGF has no such kind of effect,al- though they also increased during liver fibrosis.
5.Anti-tumor effects of a novel cyclophosphamide derivate 9b in vivo and in vitro.
Pu-Mei CUI ; Li SHU ; Fei LIU ; Jun-Qing YANG ; Yang SONG ; Wen-Juan SUN
Acta Pharmaceutica Sinica 2014;49(1):44-49
This study is to investigate the anti-tumor activities of a novel cyclophosphamide derivate 4, 6-diphenyl cyclophosphamide (9b) in vivo and in vitro, and its possible mechanism of action. The inhibitory effects of 9b on human hepatoma cell line HepG2, human breast carcinoma cell line MCF-7 and human myeloid leukemia cell line K562 were measured by MTT assay in vitro. Cell cycle distribution and apoptotic rate were evaluated by flow cytometry. To evaluate the anti-tumor effect of 9b in vivo, mouse model bearing inoculated H22 tumor was established. The results indicated that 9b could inhibit the proliferation of HepG2, MCF-7 and K562 cells in a dose and time dependent manner. The ICo50 values of 9b were 32.34 micromol.L-1 to HepG2 cells, 87.07 micromol.L-1 to MCF-7 cells and 149.10 micromol.L-1 to K562 cells after incubation for 48 h. The results of flow cytometry indicated that after being treated for 48 h with different concentrations of 9b, the ratios of HepG2, MCF-7 cells at the Go/G1 phase and K562 cells at the G0/Gl phase and G2/M phase increased significantly compared with control group, and the apoptotic rate increased with the increase of the concentration of 9b. 9b could significantly reduce tumor weight of H22 solid tumor mouse model in vivo. To summarize, 9b showed significantly anti-tumor activity in vivo and in vitro, of which the mechanism might be associated with the change of cell cycle distribution and induction of tumor cell apoptosis.
Animals
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Antineoplastic Agents, Alkylating
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chemistry
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pharmacology
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Apoptosis
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drug effects
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Cell Cycle
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drug effects
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Cell Line, Tumor
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Cell Proliferation
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drug effects
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Cyclophosphamide
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analogs & derivatives
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chemistry
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pharmacology
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Dose-Response Relationship, Drug
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Female
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Humans
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Inhibitory Concentration 50
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Liver Neoplasms, Experimental
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pathology
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Male
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Mice
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Molecular Structure
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Random Allocation
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Tumor Burden
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drug effects
6.The Outcome nalysis of microsurgical operation for treatment of intractable epilepsy
Zhi-Guo MA ; Hui YANG ; Ning AN ; Mei-Hua YANG ; Sheng-Qing LU ;
Chinese Journal of Microsurgery 2000;0(03):-
Objective To explore the therapeutic effect of patients with intractable by microsurgical operation.Methods All patients assisted by a 3-dimensional precise localization system of epileptic foei for epilepsy diagnosis and the epileptogenic focus and comprehensive surgical measures were taken for the treat- ment of isolated epileptic foci and their network.The outcomes of 50 patients in the following up 1~2 years were defined.A retrospective review was conducted.Results In this group,Excellent 53 cases (31.90%);good 83 cases(50.93%);moderate 10 cases(6.13%)and poor 18 cases(11.04%).The ef- fective rate was 80%.Conclusion The microsurgical management is an effective approach for treatment of refractory epilepsy.
7.Intervention effects of qingre jiangya capsule on brain hippocampus of spontaneously hypertensive rats based on metabonomic research.
Hai-Qing JIANG ; Lei NIE ; Yun-Lun LI ; Miao-Miao WANG ; Mei ZHU ; Wen-Qing YANG ; Xin-Ya ZHANG
China Journal of Chinese Materia Medica 2014;39(1):134-139
Thirty SHRs were obtained randomly to hypertension, model group, captopril group and Qingre jiangya capsule group. Ten Wistar rats were used as control group. The hippocampus tissue was removed to explore the damage of spontaneously hypertensive rats (SHR) and the protective effect of Qingre jiangya capsule after continuously administered for 14 days. And then the data were processed by principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA). The research results revealed captopril group was significantly different from the other three groups. The classification of other three groups is also very clear after captopril group removed. This suggested that Qingre jiangya capsule could improve the overall metabolism compared with captopril. Four metabolites were identified: dimethylglycine, glycerophosphocholine, aldosterone and noradrenaline. Hypertension hippocampus damage may mainly be expressed in tyrosine metabolism, aldosterone-regulated sodium, vascular smooth muscle contraction reabsorption, and Qingre jiangya capsule could reverse the hippocampus tissue damage of SHR.
Animals
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Capsules
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pharmacology
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Drugs, Chinese Herbal
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pharmacology
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Hippocampus
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drug effects
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Hypertension
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drug therapy
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Male
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Rats
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Rats, Inbred SHR
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Rats, Wistar
8.Research and practice of permeating the humanity quality education into pharmacology teaching
Jun-xia NG YA ; Hong-mei QIU ; Ying LUO ; Qi-xin ZHOU ; Jun-qing YANG ; Qing-song JIANG
Chinese Journal of Medical Education Research 2011;10(11):1333-1335
Enhancing medical students' humane quality education is an urgent requirement for modem medical mode transformation for medical education.The pharmacology teachers of Chongqing Medical University follow the modem education concepts and fully search the human spirit materials hidden in pharmacology,then actively explore how to integrate the humanity spirit education into the pharmacology teaching to achieve the changes of from exam-oriented education to quality education.
9.Dynamic accumulation regulation of curcumin, demethoxycurcumin and bisdemethoxyeurcumin in three strains of curcuma longae rhizome.
Qing-Miao LI ; Wen-Yu YANG ; Xue-Mei TANG ; Mei ZHANG ; Xian-Jian ZHOU ; Guang-Ming SHU ; Jun-Ning ZHAO ; Qing-Mao FANG
China Journal of Chinese Materia Medica 2014;39(11):2000-2004
The paper is aimed to study the dynamic accumulation regulation of curcumin (Cur), demethoxycurcumin (DMC) and bisdemethoxyeurcumin (BDMC) in three strains of Curcuma longa, and provide scientific references for formalized cultivation, timely harvesting, quality control and breeding cultivation of C. longa. The accumulation regulation of the three curcumin derivatives was basically the same in rhizome of three strains. The relative contents decreased along with plant development growing, while the accumulation per hectare increased with plant development growing. The accumulation of curcuminoids per hectare could be taken as the assessment standard for the best harvest time of C. longa. A3 was the best strain in terms of Cur and BDMC content.
Curcuma
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chemistry
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growth & development
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metabolism
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Curcumin
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analogs & derivatives
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analysis
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metabolism
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Quality Control
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Rhizome
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chemistry
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growth & development
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metabolism
10.Effect of syndecan-4 on the proliferation and extracellular matrix secretion of human mesangial cells stimulated by basic fibroblast growth factor
Dexuan WANG ; Qing YANG ; Ruixia LIN ; Changlin MEI ; Bing DAI ; Jiangqin LIU ; Lili FU
Chinese Journal of Nephrology 2009;25(5):375-380
Objective To investigate the effect of syndecan-4 on the proliferation and extracellular matrix (ECM) secretion of human mesangial cells(HMC) stimulated by basic fibroblast growth factor (bFGF) and to evaluate the role of syndecan-4-PKCα pathway. Methods The expression of syndecan-4 in HMC was observed by immunofluorescence. After the down-regulation of syndecan-4 in HMC by RNA interference, the cell proliferation was detected by MTT. The secretion of fibronectin (FIN), type IV collagen, type Ⅰ collagen was assessed by ELISA. The copy number of syndecan-4 and PKCα was measured by fluorescent quantitation PCR at different time points. Results Syndecan-4 was expressed in HMC. bFGF could promote the cell proliferation and ECM secretion together with the PKCα copy number per million house-keeping genes of HMC, which could be reversed by the syndecan-4 siRNA transfection (MtT: 48-60 h, P<0.01; FiN: 24 h, P<0.01, 48-96 h, P<0.05; type Ⅳ collagen: 72-96 h, P<0.05; PKCa: 0 h, P<0.05, 12-48 h, P< 0.01). Conclusion Syndecan-4 may regulate the proliferation and ECM secretion of HMC stimulated by bFGF through syndecan-4-PKCα pathway.