1.Expert's Comment.
Chinese Journal of Pediatrics 2011;49(8):616-617
Female
;
Humans
;
Male
;
Sepsis
;
complications
;
Wernicke Encephalopathy
;
diagnosis
4.Effect of diameter-controlled Ti-TiO2 nanotubes on the adhesion of osteoblast and fibroblast.
Hong-cai LI ; Yu-mei ZHANG ; Hai-ping SUN
Chinese Journal of Stomatology 2012;47(2):122-126
OBJECTIVETo study the effect of Ti-TiO2 nanotubes with different diameters on the adhesion of fibroblast and osteoblast, and to find which diameter was more favorable for cells' respective adhesion.
METHODSPure titanium sheets were polished and then anodized at different potentials for 1 h with Ti as anode and Pt as cathode. TiO2 nanotubes formed at 1, 5, 10 and 20 V potentials served as experimental groups and polished pure titanium served as control group. Field emission scanning electron microscopy (Fe-SEM) was used to analyze the surface topography. Stained nucleus with Hoechst33342 were used to measure the cell adhesion. The cell shape on the sample surface were analyzed with Fe-SEM.
RESULTSTiO2 nanotube array of different inner diameters from 15 nm to 100 nm were grown on titanium sheets by anodization at potentials from 1 to 20 V. At 30, 60 and 120 min, fibroblast adhesion at nanotubes anodized at 5 V was (141 ± 9), (388 ± 14) and (489 ± 15) respectively, significantly less than any other nanotube surface at the same time (P < 0.01). Nanotubes anodized at 20 V had the least inhibitory effect for fibroblast adhesion with a number of (579 ± 14) at 120 min, and the cell shape was also inhibited. At 30, 60 and 120 min, osteoblast had a significant better adhesion on nanotubes formed at 5 V than it did on any other surface at the same time (P < 0.01), except the control group at 30 min, with the adhesion number of (198 ± 10), (431 ± 10) and (501 ± 10) respectively, and osteoblast had a abundant spread on nanotubes formed at 5 V; while osteoblast adhesion on nanotubes anodized at 20 V was (152 ± 11), (403 ± 9) and (465 ± 12) respectively, less than on any other nanotube surface within the same time (P < 0.05), and the cell shape on the surface changed to be more elongate.
CONCLUSIONSFibroblast adhesion is inhabited more or less on Ti-TiO2 nanotubes of different diameters. Nanotubes formed at 5 V have the most osteoblast adhesion, and inhibit fibroblast adhesion.
Animals ; Cell Adhesion ; Fibroblasts ; cytology ; ultrastructure ; Mice ; Microscopy, Electron, Scanning ; Nanotubes ; chemistry ; Osteoblasts ; cytology ; ultrastructure ; Surface Properties ; Titanium ; chemistry
6.Sorting of side population cells from breast cancer MCF-7 cell line and its biological characteristics
Xin SUN ; Ping LI ; Mei ZHANG ; Jiao CHEN
Chinese Journal of Primary Medicine and Pharmacy 2012;19(13):1927-1928
Objective To separate the side population cells(SP) from breast cancer MCF-7 cell line,and observe its biological characteristics.Methods Flow cytometry and Hcechst 33342 dye efflux assay were used to isolate SP cells and non-SP cells from the MCF-7 cell line of human breast cancer.Tumorigenicity of the two subpopulations was observed by a soft agar cloning method.Results The results of FACS analysis indicated that (6.5 ± 0.4 ) %of the MCF-7 cells were SP cells;The vitro colony formation rate of SP cells was(38.5 ±9.4)%,and higher than that of non-SP cells ( 8.4 ± 2.6 ) % ( t =5.34,P < 0,05 ).Concluslon The SP cells sorted from MCF-7 cell line enriched tunor stem cells,which exhibited high tumorigenicity.It indicated that SP cells should play a principal role in breast cancer.
7.Establishment of MDCK cell models expressing human MATE1 or co-expressing with human OCT1 or OCT2.
Hong-mei LEI ; Si-yuan SUN ; Li-ping LI ; Mei-juan TU ; Hui ZHOU ; Su ZENG ; Hui-di JIANG
Acta Pharmaceutica Sinica 2015;50(7):842-847
To establish single- and double-transfected transgenic cells stably expressing hMATE1, hMATE1 cDNA was cloned by RT-PCR from human cryopreserved kidney tissue, and subcloned into pcDNA3.1(+) plasmid by virtue of both HindIII and Kpn I restriction enzyme sites. Subsequently, the recombined pcDNA3.1(+)- hMATE1 plasmid was transfected into MDCK, MDCK-hOCT1 or MDCK-hOCT2 cells using Lipofectamine 2000 Reagent. After a 14-day-cultivation with hygromycin B at the concentration of 400 µg · mL(-1), all clones were screened with DAPI and MPP+ as substrates to identify the best candidate. The mRNA content of hMATE1, the cellular accumulation of metformin with or without cimetidine as inhibitor, or transportation of cimetidine was further valuated. The results showed that all of the three cell models over expressed hMATE1 mRNA. The cellular accumulation of metformin in MDCK-hMATE1 was 17.6 folds of the control cell, which was significantly inhibited by 100 µmol · L(-1) cimetidine. The transcellular transport parameter net efflux ratios of cimetidine across MDCK-hOCT1/hMATE1 and MDCK-hOCT2/hMATE1 monolayer were 17.5 and 3.65, respectively. In conclusion, cell models with good hMATE1 function have been established successfully, which can be applied to study the drug transport or drug-drug interaction involving hMATE1 alone or together with hOCT1/2 in vitro.
Animals
;
Biological Transport
;
Cimetidine
;
pharmacology
;
DNA, Complementary
;
Dogs
;
Drug Interactions
;
Humans
;
Madin Darby Canine Kidney Cells
;
Metformin
;
pharmacology
;
Organic Cation Transport Proteins
;
genetics
;
metabolism
;
Transfection
8.Pathological observation on pulmonary arterioles in coal workers with pneumoconiosis and pulmonary heart disease.
Jun-Hua PENG ; Hong-Mei HOU ; Jing SUN ; Li-Ping YANG ; Li-Juan WANG ; Wei-Ping JIANG
Chinese Journal of Industrial Hygiene and Occupational Diseases 2006;24(11):672-672
Aged
;
Arterioles
;
pathology
;
Coal Mining
;
Humans
;
Male
;
Middle Aged
;
Pneumoconiosis
;
complications
;
pathology
;
Pulmonary Artery
;
pathology
;
Pulmonary Heart Disease
;
etiology
;
pathology
9.Dynamic Changes of Neuron-Specific Enolase mRNA and Protein in Brain Tissue of Offspring Rats with Bilirubin Encephalopathy
zhi-mei, JIANG ; xiao-jie, LI ; ai-ping, SUN ; bing-kun, CHEN
Journal of Applied Clinical Pediatrics 2004;0(12):-
Objective To observe the dynamic changes of neuron-specific enolase(NSE)mRNA and protein in offspring rats brain tissue with bilirubin encephalopathy and explore the pathological mechanism and its diagnostic value on bilirubin encephalopathy.Methods Seven-day postnatal Wistar rats were used for study.One hundred and twenty rats were divided into 2 groups randomly(control group and experimental group),which were respectively subdivided into 6 groups(6,12,24,48,72,96 h).The rats in control group were intraperitoneally administered physiological saline 0.5 mL,the rats in experimental groups were intraperitoneally administered bilirubin(200 mg/kg).Reverse transcription-polymerase chain reaction was used to analyze the dynamic changes of NSE mRNA expression at 6,12,24,48,72 and 96 h in brain tissue of rats with bilirubin encephalopathy.Immunohistochemistry was used to evaluate NSE protein expression in hippo-campi,cerebral cortex,thalamic and pallidus at different times.Results The expression of NSE mRNA significantly decreased in brain tissue of rats with bilirubin encephalopathy from 6 h to 96 h compared with the control groups.The expression of NSE protein in hippocampi decreased in offspring rats with bilirubin encephalopathy from 6 h to 96 h,but there were no differences compared with the control groups.The expression of NSE protein in cerebral cortex was significantly decreased in rats with bilirubin encephalopathy from 6 h to 96 h,there were significant differences compared with the control group.The expression of NSE protein in thalamic significantly decreased in rats with bilirubin encephalopathy from 6 h to 96 h,but there were significant differences between experimental groups and the control groups at 24 h and 72 h.The expression of NSE protein in pallidus significantly decreased in offspring rats with bilirubin encephalopathy from 6 h to 96 h,and there were significant differences compared with control groups.Conclusions The changing trends of expression of NSE mRNA were identical to those of NSE protein.NSE may reflect the degree of injury of neurogliocyte.It can serve as reliable index to determine bilirubin encephalopathy.
10.Changes of cardiac pathology in patients with cor pulmonale and coal workers' pneumoconiosis.
Hong-Mei HOU ; Jun-Hua PENG ; Jing SUN ; Wei-Ping JIANG
Chinese Journal of Industrial Hygiene and Occupational Diseases 2004;22(6):472-472
Aged
;
Anthracosis
;
complications
;
pathology
;
Cardiomyopathy, Hypertrophic
;
pathology
;
Edema
;
Humans
;
Male
;
Middle Aged
;
Myocardium
;
pathology
;
Pulmonary Heart Disease
;
etiology
;
pathology