Objective To detect multidrug resistance gene(mdr1) transcription in peripheral blood lymphocyte(PBL) before and after chemotherapy and to determine its correlation with chemotherapeutic effect.Methods The peripheral blood sample was taken from each lung cancer patients(n=32) before chemotherapy and at 3rd week after chemotherapy and subjected to fluorescent quantitative real-time RT-PCR.Simultaneously the tumor size was determined by chest X-ray or CT.Results The mdr1 mRNA level in PBL after chemotherapy increased significantly as compared with that before chemotherapy [(4.316 6?0.923 1) vs(3.881 8?0.828 0),t=-4.207,P=0.000].There was an negative correlation between mdr1 mRNA level and the tumor size(r=-0.389,P=0.028).The level of mdr1 transcription in SCLC patients was significantly less than that of NSCLC patients before chemotherapy [(2.831 4?0.398 4) vs(4.231 9?0.603 4),t=6.101,P=0.000].Conclusion In terms of an anticancer drug resistant mechanism,there could be a difference between NSCLC and SCLC.Longitudinal study of mdr1 level in PBL is valuable for evaluating the treatment(response.)

Objective:To evaluate the real-time fluorescent quantitative-polymerase chain reaction approach(fqRT-PCR) of cytokeratin 19(CK19)targets for its feasibility to estimate the number of circulating tumor cells(CTCs)in the patients with lung carcinoma during radiotherapy and to correlate our observation to clinical findings.Methods:Samples of 5ml peripheral blood were taken from each lung cancer patients(n=30)both before and after the radiotherapy course.Meanwhile tumour size was determined by chest X-ray or computed tomography.The blood samples were subjected to real time RT-PCR assay.All patients with lung cancer were treated with primary definitive and mediastinal radiotherapy.Results:Compared to with that before the-treatment,the value of CK19 mRNA in PB after therapy decreased dramaticall(y5.093 2?1.062 8 vs 4.249 3 ?0.832 3,t=3.192,P=0.007).The change of CK19 mRNA level before and after radiotherapy was closely related to the typ(esquamous cell cancer/adenocarcinoma vs.small cell cancer,0.538 9?0.903 0 vs 1.682 6?0.946 7,t=2.146 5,P=0.051).Meanwhile,there was a close link between the grade(Well/Mod vs Poor)and the change of CK19 mRNA(0.502 4 vs 1.527 1,t=2.017,P=0.065).The change of CK19 mRNA level in peripheral blood(PB)was related to variation of tumour burden during radiotherapy(r=0.057 5,P=0.025).Of the 30 cases studied,24 cases were positive before radiotherapy (24/30,80%).The positive rate was 53%(16/30)after radiotherapy,meaning that 8 cases were converted intonegative after radiotherapy. Conclusion:The disseminated cancer cells cannot be completely eradicated by radiotherapy,suggesting further more systemic adjuvant treatment should be conducted.Early assessment of the therapeutic response might be possible by serial quantitative of CTCs.Detection of tumour cells disseminated in peripheral blood can provide clinically important data that are of value for tumour staging and possibly for prognostication.

Objective:In order to develop a new standard of curative effect,this study was designed to track the number of CTCs in patients with lung cancer during chemotherapy.Methods:Samples of peripheral blood was taken from each lung cancer patients(n=32) on the day before chemotherapy as well as the third week after the chemotherapy cycle.The samples were subjected to real-time fqRT-PCR.Meanwhile the tumour size was determined by chest X-ray or computed tomograghy. Results:Compared to that of pre-chemotherapy,the expression level of CK19 in the patients significantly declined after chemotherapy(t=4.659,P=0.000 1).The level of CK19 mRNA in patients with SCLC was higher than that of patients with NSCLC(t=1.944,P=0.041).The change of CK19 mRNA correlated well with the type during the treatment.Relatively the shift of SCLC is more obvious(t=6.073,P=0.000 1).Conclusions:The real-time fluorescent quantitative-PCR approach is useful for measuring the relative number of CTCs in a patients' peripheral blood to monitor the effectiveness of treatment,and for designing more comprehensive and reasonable therapeutic regimes at earlier dates for patients.

OBJECTIVE:To investigate the characteristics and regular pattern of the adverse drug reactions (ADR) occurred in our hospital.METHODS:A total of 174 ADR cases reported by our hospital from May 2006 to June 2008 were categorized and analyzed statistically.RESULTS:The ADR were induced by 16 categories(83 kinds) of drugs,predominantly by the antimicrobial agents(12 kinds,60 cases) and Chinese drugs preparations(17 kinds,66 cases).The ADR of 57 cases(26.89%) manifested chiefly as lesions of skin and its appendages,followed by gastrointestinal lesion(39 cases,18.40%).CONCLUSION:Timely ADR reporting is conducive to ADR incidence reduction and clinical rational drug use.

Objective: To investigate the clinical value of immature reticulocyte count in early monitoring the suppression of bone marrow function in patients with chest tumor during postoperative chemotherapy.Methods:Forty-one patients with chest tumor(22 with lung cancer?19 with esophagus cancer) received chemotherapy after radical operation.Several indexes including reticulocyte count and white blood cell(WBC) were detected with sysmex SE-9000 automated analyzer during the procedure of postoperative chemotherapy.Results:Compared with the value before chemotherapy,HFR+MFR was significantly lower than that at 7 days after chemotherapy(P

OBJECTIVE: To implement the policy of New Medical Reform, and to promote pharmaceutical care of our hospital. METHODS: The policy of New Medical Reform was carried out and specific implementation methods for the improvement of pharmaceutical care of our hospital were analyzed. RESULTS & CONCLUSION: Prescription evaluation was applied to provide pharmaceutical care in the clinic indirectly. Clinical pharmacists went into the wards to provide direct pharmaceutical care. They supervised the utilization of antimicrobial drugs, carried out pharmacy propaganda and wrote Clinical Drug News. ADR monitoring was also carried out. The policy of New Medical Reform was implemented through above methods to promote pharmaceutical care of our hospital.

ObjectiveTo investigate the effect of psychological intervention on quality of life (QOL) of patients with mammary carcinoma.Methods64 patients with mammary carcinoma were divided into intervention group (n=36) and control group (n=28), and psychological status and QOL of patients of two groups were analyzed after treatment.ResultsThe psychological status and QOL of intervention group was better than that of control group (P<0.05～0.001).ConclusionThe psychological intervention in full stage being in hospital can improve QOL of patients with mammary carcinoma, and it is clinically significant to improve cure rate and survival rate of patients.

Objective To explore the clinical effect of laparoscopy combined with deep hyperthermia in the treatment of pelvic abscess.Methods Clinical data of 56 patients with pelvic abscess treated by laparoscopy combined with deep hyperthermia were retrospectively analyzed.Results 56 cases were completed in laparoscopic surgery,before surgery,after diagnostic accordance rate was 76.8%,average operation time was 51 minutes,postoperative average hospitalization was 5.1 days.After operation they were performed in vitro hyperthermia to increase the efficacy. Patients were followed up for 6 months,no recurrence.32 patients with fertility requirements of the fallopian tube,1 to 3 months after the operation of bilateral fallopian tube radiography,20 cases of patency,8 cases of tubal patency and poor.Conclusion Laparoscopy combined with deep hyperthermia in vitro is an ideal and safe treatment,which is conducive to protect the patients'reproductive function,especially for young patients with no pregnancy.

Glutamate is a major excitatory neurotransmitter in the central nervous system(CNS)and plays an important role in neuronal damage induced by cerebral ischemia. Several mechanisms contribute to modulation of glutamate release during cerebral ischemia, such as vesicular release dependent on calcium, release by reversed operation of glutamate transporters, release through swelling-activated anion channel and receptor-modulating release, etc. This review addresses the mechanisms of glutamate release during cerebral ischemia.

Objective To discuss the distribution of irregular antibodies of red blood cells and its clinical significance .Methods Micro-column gel anti-globulin technique was used to screen and identify the irregular antibodies in 6 352 patients .Results In 6 352 patients ,there were 62 cases(27 males ,35 females)having positive results in irregular antibody detection ,and the positive rate was 0 .97% .There were 54 cases having transfusion history and/or pregnancy history ,including 43 cases of irregular antibodies of Rh system(containing 34 cases of anti-E antibody) ,7 cases of MNSS system ,2 cases of Lewis system ,2 cases of Kidd system ,2 cases of P system ,1 cases of Diego system ,and 5 cases with non-specificity .All of the patients whose irregular antibodies of Rh blood group system were positive had transfusion history and/or pregnancy history .Conclusion Most of the patients with irregular anti-body positive are females ,who mostly have transfusion history and/or pregnancy history ,and the irregular antibodies of Rh system are the most common .