1.Prevalence survey on pterygium among people aged 40 and above in Hengli Town of Dongguan
Zhong-Ling, LUO ; Li-Xin, ZHANG ; Guo-Ping, ZHU ; Gan-Mei, XIANG ; Xiao-Fang, LUO
International Eye Science 2014;(7):1292-1294
AlM:To investigate the prevalence of pterygium of the household population aged 40 and above in Hengli Town of Dongguan.
METHODS: Using the method of cluster random sampling, select 3 628 people aged 40 and above in four villages and one community for visual examination, intraocular pressure check, slit lamp examination and questionnaire.
RESULTS: The actual number of subjects was 3 393 people, and examination rate was 93. 52%. We detected 843 patients with pterygium. The prevalence of pterygium was 24. 85%.
CONCLUSlON:There is high prevalence of pterygium in Dongguan area. The prevalence of pterygium is related with age and working environment, but has no relation with gender.
2.Expression and clinical significance of phosphorylated AKT and phosphatase and tensin homolog in colorectal carcinoma
Huahuang LING ; Tao LI ; Aihua LUO ; Mei GAO ; Feihong LI ; Hanguo JIANG
Cancer Research and Clinic 2014;26(4):253-256
Objective To study the expression and clinical significance of phosphorylated AKT (pAKT) and phosphatase and tensin homolog (PTEN) in colorectal carcinoma tissues.Methods The expression of pAKT and PTEN were detected by immunohistochemical SP method in 112 case of colorectal carcinomas tissue.Results The positive rates of pAKT and PTEN expression were 79.5 % (89/112) and 47.3 % (53/112) in colorectal cancerous tissues,respectively,which showed a statistically significance when compared with those in adjacent normal and adenoma tissues.The positive rate of pAKT expression was closely related with the invasive depth,clinical staging and lymph node metastasis of colorectal carcinomas.The positive expression of PTEN was also closely related with the invasive depth,degree of differentiation,clinical staging and lymph node metastasis of colorectal carcinomas.The expressions of pAKT and PTEN were negative correlaed.Conclusion The results showed that the expression of pAKT and PTEN were closely related with progression and metastasis of colorectal carcinomas,which may provide a new therapeutic target for colorectal carcinomas by blocking PTEN/PI3K/AKT signaling pathway.
3.Comparative proteomics study of different processing technology for pilose antler using iTRAQ technology coupled with 2D LC-MS.
Mengya JIN ; Ling DONG ; Yuanming LUO ; Li YU ; Mei MO ; Chengbo HOU ; Zhiyuan LI
Acta Pharmaceutica Sinica 2015;50(12):1637-44
This study was designed to use iTRAQ technology coupled with 2D LC-MS/MS to study the comparative proteomics of different processing technology for pilose antler. 1015 proteins were identified with 2D LC combined with MOLDI TOF/TOF mass spectrometry. Comparative analysis with Protein Pilot (Version 4.5) revealed that 87 proteins were changed (P ≤ 0.05, the ratio of > 1.50 or < 0.60 as the threshold selection of difference proteins), of which 24 were up regulated and 33 were down regulated in the traditional frying process (TFP) compared with the fresh pilose antler (P ≤ 0.05). 7 significant different proteins (P ≤ 0.001), most of these significantly changed proteins were found to be involved in calcium ion binding and ATP binding associated with human healthy. Freeze drying with protective agent (FDP) (Trehalose) can improve the content of significantly different proteins (P ≤ 0.001) including Collagen alpha-1 (XII) chain (COL12A1) and Collagen alpha-1 (II) chain (COL2A1). The significant function involves in platelets activating, maintenance of spermatogonium, and disorder expression in tumor cells. The functional annotation by Hierarchical clustering and GO (gene ontology) showed that the main molecule functions of the proteins significantly changed in these processes were involved in binding (52.7%), catalytic (25.3%), structural molecule and transporter (6.6%).
4.Comparative proteomics study of different processing technology for pilose antler using iTRAQ technology coupled with 2D LC-MS.
Meng-ya JIN ; Ling DONG ; Yuan-ming LUO ; Li YU ; Mei MO ; Cheng-bo HOU ; Zhi-yuan LI
Acta Pharmaceutica Sinica 2015;50(12):1637-1644
This study was designed to use iTRAQ technology coupled with 2D LC-MS/MS to study the comparative proteomics of different processing technology for pilose antler. 1015 proteins were identified with 2D LC combined with MOLDI TOF/TOF mass spectrometry. Comparative analysis with Protein Pilot (Version 4.5) revealed that 87 proteins were changed (P ≤ 0.05, the ratio of > 1.50 or < 0.60 as the threshold selection of difference proteins), of which 24 were up regulated and 33 were down regulated in the traditional frying process (TFP) compared with the fresh pilose antler (P ≤ 0.05). 7 significant different proteins (P ≤ 0.001), most of these significantly changed proteins were found to be involved in calcium ion binding and ATP binding associated with human healthy. Freeze drying with protective agent (FDP) (Trehalose) can improve the content of significantly different proteins (P ≤ 0.001) including Collagen alpha-1 (XII) chain (COL12A1) and Collagen alpha-1 (II) chain (COL2A1). The significant function involves in platelets activating, maintenance of spermatogonium, and disorder expression in tumor cells. The functional annotation by Hierarchical clustering and GO (gene ontology) showed that the main molecule functions of the proteins significantly changed in these processes were involved in binding (52.7%), catalytic (25.3%), structural molecule and transporter (6.6%).
Animals
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Antlers
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chemistry
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Chromatography, Liquid
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Collagen
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chemistry
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Down-Regulation
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Freeze Drying
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Gene Expression Regulation
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Proteomics
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Tandem Mass Spectrometry
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Technology, Pharmaceutical
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methods
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Up-Regulation
5.A meta-analysis on therapeutic efficiency of self-ligating versus conventional brackets for orthodontic correction
Yao LIU ; Yun HE ; Jie ZENG ; Feng-Ling DAI ; Yu-Ru LUO ; Jing LUO ; Mei JU
Chinese Journal of Tissue Engineering Research 2018;22(18):2946-2952
BACKGROUND: The type of brackets is closely related to the efficiency of orthodontic treatment. A self-ligating bracket is characterized by low friction, simple operation, mild pain and discomfort, and it has been an issue of concern in orthodontics. In recent years, studies on the therapeutic efficiency of self-ligating brackets versus conventional brackets for orthodontic correction have been reported, but there is no conclusion. OBJECTIVE: To systematically compare the conventional and self-ligating brackets concerning treatment efficiency. METHODS: A computer-based search of PubMed, EMBASE, MEDLINE, WanFang, VIP and CNKI was performed for randomized controlled trials concerning self-ligating brackets versus conventional brackets for orthodontic correction. The keywords were "orthodontic brackets/ self-ligating, random" in English and "appliance/self-ligating/ conventional/orthodontic correction efficiency" in Chinese. The retrieve time was set from inception to December 2017. Stata12.0 software was used for meta-analysis following literature screening, data extraction and risk assessment of bias. RESULTS AND CONCLUSION: A total of 9 randomized controlled trials involving 603 patients were included. The results of meta-analysis on the efficiency of the two groups showed that there was no significant difference in overall treatment time, number of visits, time of alignment and time of space closure (P > 0.05). Only the time of chairside operation showed significant difference between the two groups (P < 0.05). These findings indicate that compared with the conventional bracket, the self-ligating bracket has no obvious advantage in the therapeutic efficiency.
7.Puerarin decreases lens epithelium cell apoptosis induced partly by peroxynitrite in diabetic rats.
Li-Na HAO ; Yi-Qun LING ; Xiu-Mei LUO ; Yu-Xiang MAO ; Qi-Yan MAO ; Shou-Zhi HE ; Yi-Ling LING
Acta Physiologica Sinica 2006;58(6):584-592
The present study was designed to observe if puerarin decreases lens epithelium cell (LEC) apoptosis induced partly by peroxynitrite (ONOO(-)). One hundred and eight rats were randomly divided into control group (n=36), streptozotocin (STZ) group (n=36) and STZ + puerarin group (n=36). The rats in the control group intraperitoneally (i.p.) received 0.5 ml of saline. The rats in STZ group and STZ + puerarin group received intraperitoneal injection of STZ (45 mg/kg). Three days later, the rats in STZ + puerarin group were given puerarin (140 mg/kg per day, i.p.). On days 20, 40 and 60 of the experiment, morphologic changes of lenses were observed with slit lamp. Then the animals were sacrificed for further analysis. The amount and percentage of apoptotic LECs were determined by flow cytometry. Nitrotyrosine (NT, the foot print of ONOO(-)) was examined by immunohistochemistry. Apoptosis-related genes (iNOS, etc.) were analyzed by gene array. The results showed that in the control group, all the lenses were clear. In STZ group, gradually severe opacity of the lens was observed on days 20, 40 and 60. But in STZ + puerarin group, mild opacity of the lens was observed on day 20 and more severe on day 40, but markedly decreased on day 60. In the control group, mild apoptosis of LECs was observed. In STZ group, time-dependent increase in apoptosis of LECs was observed. In STZ + puerarin group, mild apoptosis of LECs was observed on day 20, significantly increased on day 40, but markedly decreased on day 60. There was no expression of NT in the lens in the control group, but an increased expression of NT in STZ group. In STZ + puerarin group, mild expression of NT was observed on day 20, significantly increased on day 40, but markedly decreased on day 60. There was no expression of iNOS in the lens in the control group, but continuous up-regulation of iNOS expression in STZ group. In STZ + puerarin group, mild expression of iNOS was observed on day 20, significantly increased on day 40, but markedly decreased on day 60. Except the changes of iNOS related to NO production, the other apoptosis-related genes, including BCL-2 and SOD were down-regulated, while NF-kappaB and TNFR1-FADD-caspase signal transduction way were up-regulated in STZ group. The results were opposite in STZ + puerarin group and the control group. These findings show that NT is expressed in diabetic rat lens, which proves that LEC apoptosis in diabetic lens is partly induced by ONOO(-) which may be a new oxidative damage way to form cataract. Puerarin partly decreases LEC apoptosis induced by ONOO(-) and is a potential medicine for therapy of diabetic cataract. The mechanism of puerarin dealing with diabetic cataract may be related to its direct inhibition of LEC apoptosis and antagonism of ONOO(-) in diabetic rats.
Animals
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Apoptosis
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Cataract
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chemically induced
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Diabetes Mellitus, Experimental
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Epithelial Cells
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drug effects
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Isoflavones
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pharmacology
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Lens, Crystalline
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cytology
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Nitric Oxide Synthase Type II
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metabolism
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Peroxynitrous Acid
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Rats
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Tyrosine
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analogs & derivatives
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metabolism
8.Analysis of misdiagnosis of two cases with lung disease.
Xiao-hong CAI ; Yi-mei JIN ; Hai-lin ZHANG ; Yun-chun LUO ; Zheng-xia ZHANG ; Zhi-guang ZHAO ; Xian-ping HUANG ; Ling-xiang JIN
Chinese Journal of Pediatrics 2005;43(6):467-468
Adolescent
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Biopsy
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Cystic Adenomatoid Malformation of Lung, Congenital
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diagnosis
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diagnostic imaging
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therapy
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Diagnosis, Differential
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Diagnostic Errors
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adverse effects
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prevention & control
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Humans
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Infant
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Lung Diseases
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diagnosis
;
diagnostic imaging
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therapy
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Male
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Middle Lobe Syndrome
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diagnosis
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diagnostic imaging
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surgery
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Tomography, X-Ray Computed
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Treatment Outcome
10.Comparative study on effects of static pressure stimulation on release of PGE2 and IL-6 in fibroblasts in the rat "Zusanli" and its adjacent areas.
Bo CHEN ; Yong-Fen LUO ; Jin CUI ; Ling-Mei FENG ; Xiao-Fang YANG ; Lin FENG
Chinese Acupuncture & Moxibustion 2007;27(2):135-140
OBJECTIVETo provide some possible theoretical and experimental basis for the modern biomedical mechanism of "receiving stimulation, preventing and treating diseases", by exploring the effects of pressure signal's biological transformation of rat "Zusanli" (ST 36) fascia tissue fibroblasts under mechanical stimulation.
METHODSPressure was given on rat "Zusanli" (ST 36) and its adjacent area's fascia tissue cells cultured in vitro and identified by morphology. The contents of prostaglandin E2 (PGE2) and interleukin-6 (IL-6) in culture medium were detected.
RESULTSThe fascia tissue cells in "Zusanli" and its adjacent area are almost consisted of fibroblasts. The pressure stimulation significantly accelerated the synthesis and release of PGE2 and IL-6.
CONCLUSIONAcupoint and non-acupoint fibroblasts can directly receive mechanical stimulation, and then the mechanical signals were transformed as biological ones.
Acupressure ; Acupuncture Points ; Animals ; Dinoprostone ; secretion ; Fascia ; cytology ; Fibroblasts ; secretion ; Interleukin-6 ; secretion ; Male ; Rats ; Rats, Sprague-Dawley ; Signal Transduction ; physiology