Objective To analyze the effects and applications of 4 kinds of bioreactors on cell culture. Methods The results of different bioreactors for cell culture were observed. Results The bioreactors with different mechanical properties, mass transfer and fluid shear stress had different impacts on morphological and functional organizations. Conclusion Rotating bioreactor is more suitable for three-dimensional suspension growth and similar to organisms internal environment than the others. It is indicated that bioreactors will be integrated intelligent devices including culture, control, research, and production in the future.

Objective To assess the status of self-esteem of college students of higher vocational medical school and to provide targeted guidance.Methods Totally 1014 college students of higher vocational medical school were assessed using self-esteem scale and the results were analyzed by SPSS 13.0.Results The self-esteem levels among different student levels were different.The self-esteem level was lower in students of technical secondary school than in students of vocational college and junior college.The self-esteem levels were different among factors of different grades,family location,duty and post,satisfaction degree for school,individual health and family education (P ＜ 0.05 ) ;while they are not significantly different among factors of gender,major,being singleton or not and family types ( P ＞ 0.05 ).Conclusions School should further strengthen the mental health education and use kinds of methods to improve the self-esteem of college students.

Objective To evaluate clinical characteristics and prognostic factors of synchronous multiple primary esophageal carcinomas (SMPEC).Methods Clinical data including sex,age,smoking history,regions,lymph node status,length of tumor,therapeutic methods and survival data of 52 patients with SMPEC were analyzed.The rates of OS depending on the different factors were calculated using the method of Kaplan-Meier analysis.Log-rank test was used for univariate survival analysis and Cox's proportional hazards regression model was used for multivariate survival analysis.Results The 1-,3-,5-year OS rates and the median survival time (MST) were 65.4 ％,17.3 ％,7.7 ％ and 15.0 months for the whole cohort,75.0 ％,33.3 ％,16.7 ％ and 19.5 months for the surgery subgroup,62.5 ％,10.0 ％,5.0 ％ and 14.5 months for the 40 non-operative patients.Multivariate analysis of prognostic factors identified that tumor length and M stage were independent prognostic factors for the whole cohort,while tumor length,M stage and chemotherapy were independent prognostic factors for the radiotherapy subgroup patients.Conclusion The tumor length and M stage are independent prognostic factors for the SMPEC patients.Combined radiotherapy with chemotherapy seems to bring survival benefit and maybe a better management choice for unresectable and non-operative SMPEC.

Cancer is one of the three major killers that threatened human health in the twenty-first century,making the early diagnose and high-efficiency therapy crucial.The recent-developed nanotechnology brings new hope for this.Among different nanomaterials,magnetic nanoparticles show positive effects in diagnosis and therapies since they can be located with certain magnetic fields and can vibrate and heat up in the alternating magnetic field.With the ever increasing attention paid to the study,magnetic nanoparticles will play a key role in cancer diagnosis and therapy.This review gives a comprehensive discussion on development of the research and applications of magnetic nanoparticles,including single metallic,double metallic and alloyed magnetic nanoparticles.Moreover,it also prospects the future developing orientations of magnetic nanotechnology.Magnetic nanoparticles are widely used these days,with a speedy research development both in theory and application.However,the investigations into the toxicity of magnetic nanoparticles and the medical and commercial standards have to continue before magnetic nanoparticles are determined as valid and with low toxicity.

Objective: To know the occasion and how the military in-patients understood the contents of health education, to discuss how to conduct the health education pertinently and how to make the military in-patients master the information and skill to get well effectively in a short time. Methods: By referring to literature samples and the main contents of health education of sections in our hospital, we designed a questionnaire including the main contents of health education、the fashion and the occasion of conduction in different phase of hospitalization, together with three subjects and 44 items, and we investigated 312 military in-patients in the way of questionnaire in combination of opening and closing. Results: Military in-patients considered the correlative knowledge of getting well as the shoe pinches of the main contents of health education. The communication between doctors、nurses and pation should be strengthened; Communication through talking and offering written materials were the two primary fashions of health education; The medical personnel and military in-patients disagreed in the time arranging of health education. Conclusion: We suggest the adoption of the educational method of ”advantage health education contents increased by degrees” to conduct health education; paramedic in all levels should pay attention to the feelings of military in patients, pay attention to the rational arrangement of the health education time and contents. We emphasize the training of paramedic's communication skills of talking and writing.

Objective To investigate and analyze the status quo of job satisfaction of nurses in a large 3-A-grade hospital in Tianjin. Methods Using stratified random sampling method, the general information questionnaire and the nurse job satisfaction scale were used to investigate the cross-sectional survey of 435 clinical nurses in large 3-A-grade hospital in Tianjin. Results The score of job satisfaction was (123.54±15.52) points, which was at the middle level. According to single factor analysis, there were significant differences in the influence of nurses′ job satisfaction on age, working life, department, monthly average night shift frequencies, monthly gross income (F=2.238-7.622, all P<0.05). There was a significant difference in job satisfaction among nurses with different income(F=2.642, P=0.049), among whichwork load,relationship with colleaguesandjob itselfhad the most significant impact on job satisfaction(F=2.667, 6.087, 4.023, all P<0.05). Conclusions Nursing managers should be rational allocation of human resources, reduce the workload of nurses, to build a harmonious atmosphere in the team, to create a good working environment, provide a better career development platform for nurses, implement flexible management, improve nurses′job satisfaction.

Humans ; Lichen Planus, Oral ; therapy ; Outcome Assessment (Health Care) ; Treatment Outcome

Adult ; Chromosome Aberrations ; Female ; Humans ; Infant, Newborn ; Karyotyping ; Male ; Pedigree

OBJECTIVE: To establish an effective HPLC method for determinating the contents of salviaflaside and rosmarinic acid in Prunella vulgaris. METHODS: An Agilent Eclipse XDB-C18 column (4.6 mm × 250 mm, 5 μm) was used with gradient mobile phase of acetonitrile-water(1.0% acetic acid) at a flow rate of 1.1 mL · min-1. UV detection wavelength was 319 nm, and the column temperature was 30°C, the sample injection volume was 10 μL. RESULTS: Good linearity was obtained in the range of 0.0067-4.2 μg and 0.047-9.4 μg for salviaflaside and rosmarinic acid, respectively. The method recoveries of salviaflaside and rosmarinic acid were 99.99% and 100.2%, RSDs were 1.13% and 1.64%, respectively. The contents of salviaflaside and rosmarinic acid were more than 0.1‰ and 1%, respectively. CONCLUSION: The HPLC method is simple, rapid, accurate and suitable for the determination of salviaflaside and rosmarinic acid, which can be used for the quality control of Prunella vulgaris L. Copyright 2012 by the Chinese Pharmaceutical Association.