1.Promote multidisciplinary study on anthracycline-induced cardiotoxicity in children.
Shu-bao CHEN ; Mei-rong HUANG ; Jing-yan TANG
Chinese Journal of Pediatrics 2013;51(8):565-568
Anthracyclines
;
administration & dosage
;
adverse effects
;
Antineoplastic Agents
;
administration & dosage
;
adverse effects
;
Biomarkers
;
blood
;
Cardiomyopathies
;
chemically induced
;
diagnosis
;
prevention & control
;
Cardiotonic Agents
;
therapeutic use
;
Child
;
Child, Preschool
;
Echocardiography
;
Heart
;
drug effects
;
Heart Diseases
;
chemically induced
;
diagnosis
;
prevention & control
;
Humans
;
Risk Factors
;
Survival Analysis
;
Troponin I
;
analysis
2.Correlation between p53 gene mutations and p53 protein overexpression in esophageal squamous cell carcinoma.
Guang LI ; Zhao-xia LIU ; Jing HUANG ; Jun-mei WANG ; Quan-hong WANG
Chinese Journal of Pathology 2005;34(12):802-804
Adult
;
Aged
;
Carcinoma, Squamous Cell
;
genetics
;
metabolism
;
Esophageal Neoplasms
;
genetics
;
metabolism
;
Female
;
Gene Expression Regulation, Neoplastic
;
Genes, p53
;
Humans
;
Loss of Heterozygosity
;
Male
;
Middle Aged
;
Point Mutation
;
Tumor Suppressor Protein p53
;
genetics
;
metabolism
3.Quantitative models of Raman spectroscopy for five kinds of traditional Chinese medicine containing CaCO3 based on an improved siPLS.
Long CHEN ; Ming-yang YUAN ; Jing MING ; Yi-mei LIU ; Bi-sheng HUANG ; Ke-li CHEN
China Journal of Chinese Materia Medica 2015;40(18):3608-3615
The aim of this paper is to apply Raman spectroscopy technique to develop rapid quantitative models for five kinds of Traditional Chinese Medicine containing CaCO3. In the experiment, Raman spectras of 67 batch of sample including Otolithum Sciaenae, Galaxeae Os, Ophicalcitum, Calcite, Stalactite and their mixture which had different content of CaCO3 were collected, and the quantitative models were established by using an improved siPLS to optimize the characteristic spectral bands and using the CaCO3 contents which were measured by EDTA titration method as references. Compared with the results by EDTA titration, the established quantitative model for CaCO, content showed a prediction result that the average relative deviation of the prediction results is 2. 71% and the average recovery rate was 100.46%, when the content is between 0.465 4-0.999 7, and when the characteristic spectral bands of 1 290-1 280, 730-714, 700-690, 660-650, 465-460, 455-445, 405-385 cm(-1) had been optimized. The result also showed that the model using Raman spectroscopy and based on an improved siPLS can get a rapid determination for contents of 5 kinds of Traditional Chinese Medicine containing CaCO3.
Calcium Carbonate
;
chemistry
;
Drugs, Chinese Herbal
;
chemistry
;
Least-Squares Analysis
;
Models, Statistical
;
Plants, Medicinal
;
chemistry
;
Spectrum Analysis, Raman
;
methods
4.Effect of ERK1/2 on rat pulmonary artery smooth muscle cells Kv1.5 channel in the process of hypoxia.
Yuan-ypan WANG ; Meng-xiao ZHENG ; Mei-ping ZHAO ; Lin-jing HUANG ; Wan-tie WANG
Chinese Journal of Applied Physiology 2015;31(5):418-426
OBJECTIVETo explore the effect of ERK1/2 MAPK pathway on the expression of Kv1.5 channel, a voltage-gated potassium ion channel, in rat pulmonary artery smooth muscle cells (PASMCs) and its mechanisms during the process of hypoxia.
METHODSThe PASMCs derived from SD rats were cultivated primarily. The third to sixth generation of PASMCs were divided into 5 groups randomly: (1) Normal group (N); (2) Hypoxic group (H); (3) Demethy sulfoxide(DMSO) group (HD); (4) U0126 group (HU): 10 micromol/L U0126; (5) Anisomycin group (HA): 10 micromol/L anisomycin. There were three dishes of cells in each group. The cells in normal group were cultured in normoxic incubator (5% CO2, 37 degrees C), the cells in other groups were added to 0.05% DMSO in the hypoxic incubator (5% CO2, 2% O2, 37 degrees C), all cells were cultured for 60 h. RT-PCR and Western blot were used to detected the espressions of Kv1.5 mRNA and protein in PASMCs.
RESULTSCompared with N group, the expressions of Kv1.5 mRNA and protein in H, HD and HA groups were reduced significantly (P < 0.05); Compared with H group and HD groups, Kv1.5 mRNA and protein expressions in HU group were increased sharply (P < 0.05). Compared with the HU group, Kv1.5 mRNA and protein expressions in HA groups were significantly lower (P < 0.05).
CONCLUSIONLow oxygen reduced Kv1.5 mRNA and protein expressions, U0126 could resistant the Kv1.5 channel lower expression caused by hypoxia. Anisomycin had no significant effect on Kv1.5 channel expression under hypoxia, but the expression of Kv1.5 was still significantly lower than the normal oxygen group. These data suggest that hypoxia may cause hypoxic pulmonary hypertension by interfering ERK1/2 signaling pathway to inhibit Kv1.5
Animals ; Cell Hypoxia ; Hypertension, Pulmonary ; Kv1.5 Potassium Channel ; metabolism ; MAP Kinase Signaling System ; Mitogen-Activated Protein Kinase 1 ; metabolism ; Mitogen-Activated Protein Kinase 3 ; metabolism ; Muscle, Smooth, Vascular ; cytology ; Myocytes, Smooth Muscle ; metabolism ; Oxygen ; Pulmonary Artery ; cytology ; RNA, Messenger ; Rats ; Rats, Sprague-Dawley
5.Model index observations in SIVmac251-infected rhesus macaques.
Yu ZHANG ; Jing WANG ; Xiang-mei LIU ; Fan-gui MIN ; Peng-jv GUO ; Ren HUANG
Chinese Journal of Virology 2014;30(6):675-682
In this study, five rhesus macaques were inoculated intravenously with SIVmac251 to establish a model of simian autoimmune deficiency syndrome (SAIDS). Peripheral blood samples were collected at different time points to monitor changes in the total T cell number and T lymphocyte subset. Plasma viral loads, cytokine expression levels and anti-SIV antibody levels were also assayed to acquire certain basic indexes to evaluate disease progression in the rhesus macaque SAIDS model. During the acute stage of infection, plasma viral loads reached a peak at week 1 post-inoculation and lasted for approximately 3 to 44 weeks. The CD3+ CD4+ T lymphocyte count in peripheral blood also transitorily decreased. During the same period, the level of interferon-gamma show an increasing trend, whereas IL-12 levels decreased; IL-2, IL-4, IL-10 and TNF-alpha were maintained at normal levels or could not be detected. During the asymptomatic and ARC phases, plasma viral loads persisted above 10(4) RNA copies/mL and either increased or declined during the later stages of disease; CD3+ CD4+ counts showed a steadily declining trend and the ratio of CD4 to CD8 decreased during late-stage disease. Moreover, antibodies against viral proteins were detected in the plasma and showed a significant increasing trend, while there were no apparently changes in the levels of IFN-gamma, IL-12, IL-2, IL-4, IL-10 and TNF-alpha. In conclusion, the characteristics of the SIV animal models in our study are similar to those of patients with AIDS. Therefore, the rhesus macaque SIVmac251 infection models can be applied for further studies into AIDS.
Animals
;
Antibodies, Viral
;
blood
;
CD4 Lymphocyte Count
;
CD4-Positive T-Lymphocytes
;
virology
;
Cytokines
;
genetics
;
immunology
;
Disease Models, Animal
;
HIV Infections
;
genetics
;
immunology
;
virology
;
HIV-1
;
physiology
;
Humans
;
Macaca mulatta
;
Male
;
Simian Acquired Immunodeficiency Syndrome
;
genetics
;
immunology
;
virology
;
Simian Immunodeficiency Virus
;
physiology
;
Viral Load
6.Isolation, identification and genetic analysis of a murine norovirus strain.
Wen YUAN ; Yu ZHANG ; Jing WANG ; Xiang-Mei LIU ; Wei-Bo ZHAO ; Ren HUANG
Chinese Journal of Virology 2014;30(4):359-368
Murine norovirus (MNV) was first discovered in mice in 2003. MNV is a member of the genus Norovirus in the family Caliciviridae. It is one of the most important and prevalent pathogens of laboratory mice, and almost all mouse strains are susceptible to MNV infection. In this study, a MNV strain was isolated from the cecal contents of infected mice and identified by the cytopathic effect (CPE) assay, virus plaque assay, 50% tissue culture infectious dose (TCID50) assay, electron microscopy, indirect immunofluorescence assay (IFA) and nucleotide sequencing. On infection, the RAW264.7 cell line showed obvious cytopathic effects within 24 to 48 hours post-inoculation, as infected cells became rounded, bright and shrunken, with ultimate disintegration of the cell sheet. After the isolation of the MNV virus, the virus was plaque-purified in RAW264.7 cells. The TCID50 of the virus was 10(5.25/0.1 mL. Electron microscopic observations of the purified virus showed the presence of spherical and non-enveloped viral particles that were 30 to 35 nm in diameter. According to the identification results, the isolate was named as MNV Guangzhou/K162/09/CHN. Thereafter, five overlapping gene fragments that covered the entire open reading frame (ORF) were amplified by RT-PCR, and the 3'-untranslated region (UTR) and 5'-UTR were amplified using the 3'-rapid amplification of cDNA ends (RACE) and the 5'-RACE method, respectively. Each of the gene fragments were cloned and sequenced, and whole genome sequences of the strain were obtained by assembling the cDNA fragment sequences. The results showed that the length of the complete genome was 7 380 nucleotides (GenBank accession number: HQ317203). The comparison of nucleotide and deduced amino acid sequences of the isolate was performed against other MNV strains in the GenBank database. A phylogenetic tree based on VP1 nucleotide sequences was constructed using MEGA5.0 software. The homology of nucleotides between the MNV Guangzhou/K162/09/CHN strain and other MNV isolates ranged from 87.4% to 89.7%. Phylogenetic analysis showed that there was a close genetic relationship between the Guangzhou/K162/09/CHN strain and MNV strains isolated from Japan (S7-P2 and S7-PP3 isolates), Korea (K4 isolate), and Germany (Berlin/04/06/DE and Berlin/05/06/DE isolates). This is the first report of the isolation and identification of MNV in China, and the first report of the genetic analysis of its complete genome.
Animals
;
Caliciviridae Infections
;
veterinary
;
virology
;
Mice
;
Molecular Sequence Data
;
Norovirus
;
classification
;
genetics
;
isolation & purification
;
physiology
;
Open Reading Frames
;
Phylogeny
;
Rodent Diseases
;
virology
;
Sequence Homology, Amino Acid
;
Viral Proteins
;
chemistry
;
genetics
7.Regulative mechanisms of mammalian target of rapamycin signaling pathway in glomerular hypertrophy in diabetic nephropathy and interventional effects of Chinese herbal medicine.
Jing-Jing YANG ; Yan-ru HUANG ; Yi-gang WAN ; Shan-mei SHEN ; Zhi-min MAO ; Wei WU ; Jian YAO
China Journal of Chinese Materia Medica 2015;40(16):3125-3131
Glomerular hypertrophy is the main pathological characteristic in the early stage of diabetic nephropathy (DN), and its regulatory mechanism is closely related to mammalian target of rapamycin (mTOR) signaling pathway activity. mTOR includes mTOR complex 1 (mTORC1) and mTOR complex 2(mTORC2), in which, the upstream pathway of mTORC1 is phosphatidylinositol-3-kinase (PI3K)/serine-threonine kinase(Akt)/adenosine monophosphate activated protein kinase(AMPK), and the representative signaling molecules in the downstream pathway of mTORC1 are 4E-binding proteins(4EBP) and phosphoprotein 70 S6Kinase(p70S6K). Some Chinese herbal extracts could improve cell proliferation via intervening the expressions of the key molecules in the upstream or downstream of PIK/Akt/mTOR signaling pathway in vivo. As for glomerular mesangial cells(MC) and podocyte, mTOR plays an important role in regulating glomerular inherent cells, including adjusting cell cycle, energy metabolism and matrix protein synthesis. Rapamycin, the inhibitor of mTOR, could suppress glomerular inherent cell hypertrophy, cell proliferation, glomerular basement membrane (GBM) thickening and mesangial matrix deposition in model rats with DN. Some Chinese herbal extracts could alleviate glomerular lesions by intervening mTOR signaling pathway activity in renal tissue of DN animal models or in renal inherent cells in vivo and in vitro.
Animals
;
Diabetic Nephropathies
;
drug therapy
;
enzymology
;
genetics
;
pathology
;
Drugs, Chinese Herbal
;
administration & dosage
;
Humans
;
Hypertrophy
;
drug therapy
;
enzymology
;
genetics
;
pathology
;
Kidney Glomerulus
;
drug effects
;
metabolism
;
pathology
;
Signal Transduction
;
drug effects
;
TOR Serine-Threonine Kinases
;
genetics
;
metabolism
8.Clinical analysis of thoracoscopy of 30 coalworker's pneumoconiosiswith pleural effusion cases.
Yandong LIANG ; Ruiling JIANG ; Chunxiao YU ; Cheng HUANG
Chinese Journal of Industrial Hygiene and Occupational Diseases 2015;33(7):541-543
OBJECTIVETo investigate the diagnostic value of thoracoscopy on idiopathic coalworker's pneumoconiosis with pleural effusion in general medicine.
METHODRoutine (general medicine) thoracoscopyof patients suffering from iIdiopathiccoalworker's pneumoconiosis with pleural effusion, pathological examination of lesions obtained (direct vision).
RESULTPathological examination revealed grayish-white miliary nodules with multiple protruding nodules, irregular focal pleura thickening, pulmonary congestion, edema, fibrous adhesion. Thorascopy produced a diagnostic rate of 93.3%. Confirmed cases includes 13 cases of tuberculous pleurisy, 11 cases of malignant pleural effusion, 4 cases of cardiac insufficiency with pleural effusion and 2 cases of idiopathic pleural effusion, with no serious complications.
CONCLUSIONThoracoscopy of idiopathic coalworker's pneumoconiosis with pleural effusion is a safe, accurate diagnostic methodin general medicine, and could benefit the establishment of a treatment method quickly, visual observation of the lesions of patients suffering from coalworker's pneumoconiosis with pleural effusion using thoracoscopy, and at the same time offer preliminary investigationof the correlation between the intensity and compactibilityof coal macule distribution and clinical stages of coalworker's Pneumoconiosis.
Anthracosis ; diagnosis ; Heart Failure ; diagnosis ; Humans ; Lung ; pathology ; Pleural Effusion ; diagnosis ; Pleural Effusion, Malignant ; diagnosis ; Pulmonary Edema ; diagnosis ; Thoracoscopy ; Tuberculosis, Pleural ; diagnosis
9.Epidemiologic features of Kawasaki disease in Shanghai from 2003 through 2007.
Xiao-jing MA ; Cen-yan YU ; Min HUANG ; Shu-bao CHEN ; Mei-rong HUANG ; Guo-ying HUANG ; null
Chinese Medical Journal 2010;123(19):2629-2634
BACKGROUNDThe epidemiologic pictures of Kawasaki disease (KD) in Shanghai from 1998 through 2002 were reported, while the current status of KD in the following five years remains unknown.
METHODSA questionnaire form and diagnostic guidelines for KD were sent to 50 hospitals providing pediatric medical care in Shanghai, China. All patients with KD diagnosed during January 2003 through December 2007 were recruited.
RESULTSIn total, 1187 cases of KD were enrolled. The incidence of KD was 36.78 to 53.28 (mean 46.32 ± 6.51) per 100 000 children under the age of 5 years between 2003 and 2007, which was higher than the year from 1998 to 2002 of (27.32 ± 7.11) per 100 000, (t = 4.406, P = 0.002). Ages at onset ranged from 12 days to 13.6 years (median 1.8 years). It occurred more frequently in summer and spring. Coronary arterial lesions (CAL), defined as ectasia or aneurysm, accounted for 19.8% (232 cases). Flattened or inverted T wave was the most frequent finding (194 cases, 20.5%) by electrocardiogram. Intravenous gamma-globulin was administrated to 1028 cases (86.6%). The occurrence of CAL seemed less frequent in the patients received gamma-globulin from day 5 to day 9 after the onset with the regimen of 1000 mg/kg once or 1000 mg/kg twice.
CONCLUSIONSThe incidence of KD was increasing in Shanghai. Treatment with intravenous gamma-globulin from day 5 to day 9 after the onset with the regimen of 1000 mg/kg once or 1000 mg/kg twice resulted in less coronary sequelae.
Adolescent ; Age of Onset ; Child ; Child, Preschool ; China ; epidemiology ; Female ; Humans ; Infant ; Infant, Newborn ; Male ; Mucocutaneous Lymph Node Syndrome ; epidemiology ; Surveys and Questionnaires
10.Construction, fermentation and purification of high polymer spider dragline silk protein containing RGD peptide.
Chao-Ran RUAN ; Jing-Xing HUANG ; Mei-Hong WEI ; Min LI
Chinese Journal of Biotechnology 2007;23(5):858-861
Spider silk is a natural protein fibroin with excellent character as it is light and tenacious. It has a wild potential applications in the biomedical field due to its good biocompatibility and degradation. Arginine-glycine-aspartic acid (RGD) is a highly conserved amino acid sequence of many adhesion protein. Biological materials binding with RGD peptide in the surface can promote cells adhesion, migration and proliferation. Our lab had constructed the 16 muhimers with the introduced RGD peptide codons which involve cell adhesion for the first time. It was found that the mechanical capability of the 16 mulimer protein was very limited because of the big gap in molecular weight with nature spider proteins when it was used to made biomaterial scaffold.In this paper,based on the 16 multimers of the highly, repetitive sequence of spider dragline silk and with RGD peptide condons which has been constructed by our lab forestall, it was used to construct the 32 and 64 multimers sequence of spider dragline silk by the strategy of "head to tail". The 32 and 64 multimers were ligated into prokaryotic expression vector pET-30a, and then the B121 (DE3) pLysS. The fragments were in agreement with the desired through digestion, agarose gel electrophoresis respectively. By registration into the GenBank data-base, the serial numbers of DQ469929 and DQ837297 were gained respectively. The expression of recombinant protein was introduced by the addition of IPTG. SDS-PAGE analysis shows that the molecular weight of products expressed here are 102 kD and 196.6kD in agreement with the desired respectively. It was the first time for the high polymer spider dragline silk protein expressed in prokaryotic biology. Furthermore, a larger quantity of synthetical proteins with high density fermentation were searched after, and a suit of high efficient purification methods for 32 multimers protein were established.
Animals
;
Escherichia coli
;
genetics
;
metabolism
;
Fermentation
;
Fibroins
;
biosynthesis
;
genetics
;
Molecular Sequence Data
;
Oligopeptides
;
biosynthesis
;
chemistry
;
genetics
;
Polymers
;
metabolism
;
Protein Engineering
;
Recombinant Proteins
;
biosynthesis
;
genetics
;
isolation & purification
;
Transformation, Bacterial