1.Therapeutic effect of botulinum toxin A to muscle spasticity after cerebral vascular diseases
Mei ZHANG ; Zhixiang LI ; Jialan DAI
Journal of Clinical Neurology 1995;0(04):-
Objective To observe the therapeutic efficacy and the safety on botulinum toxin A (BTX A) to muscle spasticity after cerebral vascular diseases (CVD).Methods 42 patients with muscle spasticity after CVD were injected with Chinese type A botulinum toxin(CBTX A).The dosage and number of injection point were determined according to the affected musclar size and the degree of muscle spasms.The injection sites were determined according to anatomy surface landmarks for location.The myodynamia of the limbs,the muscular tone (modified Ashworth score) and the function before and after injection were observed.Results The muscular tone modified Ashworth score,pace velocity,stride length and ankle ROM were remarkbly progressive after injection(P
2.Construction of culturing objective and curriculum system for rural order directed free medical students
Hui GAO ; Wuxuan MEI ; Dai LI ; Shaohua PENG
Chinese Journal of Medical Education Research 2013;(7):657-659
Culturing objective and curriculum system for rural order directed free medical students were proposed scientifically and detail measure in designing general course module , basic course module, clinical course module, preventive care course module and professional practice module were put forward based on the analysis of guiding ideology of training rural order directed free medical educational talents.
3.Anti-inflammatory and analgesic effects in vivo and bacteriostasis effect in vitro of diuretic mixture
Wenxian GUO ; Weibo DAI ; Quanxi MEI ; Hongnian LI
Drug Evaluation Research 2017;40(2):201-205
Objective To investigate the analgesic and anti-inflammatory effects of diuretic mixture and its bacteriostasis effect in vitro,and to provide scientific basis for clinical application.Methods Ear swelling test induced by xylene,twisting reaction test induced by acetic acid,capillary permeability increase in abdominal cavity of mice induced by acetic acid,and pain test induced by formalin were used to observe the analgesic and anti-inflammatory effects of diuretic mixture at high,middle and low doses (crude drug 18.0,9.0,and 4.5 g/kg).Bacteriostatic activities of diuretic mixture were tested by K-B paper disc diffusion method.Results Diuretic mixture alleviated ear edema in mouse model at high dose (P < 0.01).Diuretic mixture at high,middle,and low dose could effectively decrease the twisting reaction (P < 0.01),inhibit capillary permeability (P < 0.05 or 0.01),and ease the ache degree of mice induced by formalin in the first phase (P < 0.01),but there was no significant difference in the degree of pain intensity of phase Ⅱ.The inhibitory rates of diuretic mixture on Staphylococcus aureus and Escherichia coli were 95.04% and 37.44%,respectively.Conclusion Diuretic mixture has significant effects on analgesia and anti-inflammation and against S.aureus and E.coli in vitro.
4.Clinical characteristics of ventricular preexcitation dilated cardiomyopathy induced by accessory pathway
Chencheng DAI ; Wenxiu LI ; Yanyan XIAO ; Mei JIN ; Ling HAN
Chinese Journal of Applied Clinical Pediatrics 2014;29(9):683-686
Objective To discuss the characteristics,diagnosis,mechanism and the prognosis of dilated cardiomyopathy (DCM) induced by accessory pathway (AP).Methods The clinical,electrophysiological and echocardiographic characteristics in four cases,who were diagnosed as AP-induced DCM in the Department of Pediatric Cardiology of Beijing Anzhen Hospital,Capital Medical University from Mar.2011 to Aug.2012,were analyzed before and after ablation.Results The electrocardiograms of the 4 patients all indicated type B ventricular preexcitation.The locations of the APs were the right-sided anteroseptum and the free wall.Dyschronous contractions between posterior wall of left ventricle and interventricular septum were demonstrated by M-Mode echo.The basal segments of the interventricular septum turned thin and moved similar to an aneurysm,with typical bulging during end-systole,which was observed in all cases by two-dimension echo.Dyschronous left ventricular contraction was shown by speckle tracing technique.All patients received successful radio frequency current ablations.Their physical activities and growth improved greatly in the 4 cases.The echocardiographic data demonstrated that their left ventricular contraction recovered to synchrony shortly after the ablation,left ventricular ejection fraction recovered to normal and left ventricular end diastolic diameter decreased to almost normal gradually during the follow-up.Conclusions Overt right-sided APs localized in anteroseptum or free wall may have adverse effects on ventricular wall motion and left ventricular function.They can even result in DCM.Dyssynchronous ventricular contraction induced by right-sided overt accessory pathway may be the vital mechanism.AP-induced DCM is an indication for ablation with good prognosis.
5.Relationship between the protective effect of sodium oxybate on neuronal damage induced by hypoxia-reoxygenation and GABA_A receptor in primary cultured rat cortical neurons
Shuling GU ; Mei LI ; Jiayong CUI ; Bin YAO ; Tijun DAI ;
Chinese Journal of Clinical Pharmacology and Therapeutics 2002;0(05):-
AIM: To investigate the relationship between the protective effect of sodium oxybate on neuronal damage induced by hypoxia reoxygenation and GABA A receptor in primary cultured rat cortical neurons. METHODS: The primary cultured rat cortical neurons were used to make the hypoxia reoxygenation damage model. The morphology of cell was observed. The lactate dehydrogenase (LDH) effluxed into the media as an indicator of neuronal injury was detected after 6 h of the reoxygenation injuries. The malonyldialdehyde (MDA) contents, superoxide dismutase (SOD) and glutathione peroxidase (GPX) activities were determined at the same time. RESULTS: The hypoxia reoxygenation caused neuronal swelling and widespread neuronal degeneration, increased LDH efflux and MDA contents, and decreased SOD and GPX activities. Sodium oxybate assuaged neuron damage, decreased LDH efflux and MDA contents (P
6.Bone SPECT and CT fusion imaging in pediatric neuroblastoma
Wei, DONG ; Mei, LI ; Hao-jie, DAI ; Hui-dong, CHEN
Chinese Journal of Nuclear Medicine 2011;31(6):386-389
ObjectiveTo evaluate the diagnostic performance of bone SPECT and CT fusion imaging in bone metastases from pediatric neuroblastoma.MethodsTwenty-four pediatric patients with neuroblastoma were included in this retrospective study.All patients underwent planar imaging and SPECT integrated with CT.Lesion visibility,diagnostic certainty and diagnostic performance were evaluated with KolmogorovSmirnov test andx2 test.ResultsLesion visibility of SPECT alone,SPECT integrated with CT were significantly better than that of planar imaging ( both H =69.000,P < 0.05 ).SPECT and CT fusion imaging,SPECT alone both detected five more bone lesions than planar bone imaging (77 vs 72).The diagnostic accuracy of SPECT imaging (62.34%,48/77 )was significantly higher than that of planar imaging (45.45%,35/77; x2 =4.416,P < 0.05 ).The sensitivity,specificity and accuracy of SPECT and CT fusion imaging for diagnosing malignant bone lesions were significantly higher than those of planar imaging:82.35% (42/51) vs 53.19% ( 25/47),88.46% ( 23/26 ) vs 40.00% ( 10/25 ),84.42% ( 65/77 ) vs 45.45% (35/77 ; x2 =12.571,14.016,25.667,all P < 0.01 ).The diagnostic specificity and accuracy of SPECT and CT fusion imaging were significantly higher than those of SPECT alone ( 53.85%,14/26 ;62.34%,48/77) (x2 =7.589,9.606,both P <0.01 ).However,there was no significant difference of sensitivity between the two methods (x2 =2.942,P > 0.05 ).Diagnostic certainty by SPECT and CT fusion imaging was significantly higher than that by SPECT alone ( H =28.000,P < 0.05 ) and by planar imaging (H =21.000,P < 0.05).ConclusionSPECT and CT fusion imaging can detect more bone lesions in patients with pediatric neuroblastoma.It is helpful for diagnosing bone metastases from pediatric neuroblastoma.
7.Volatile Oil Constituents of Two Acremonium Endophyte Isolates from Aquilaria sinensis
Xiu-Huan ZHANG ; Wen-Li MEI ; Hao-Fu DAI ;
Microbiology 1992;0(01):-
The volatile oils of endophyte isolates R1 (Acremonium sp.) and R2 (Acremonium sp.) from Aqui-laria sinensis were extracted by solvent method. Twenty and sixteen ingredients were identified from the volatile oils of R1 and R2 by GC-MS, respectively. Fatty acids, such as oleic acid, linoleic acid, hexa-decanoic acid etc., were the main components, whereas terpenoids like 1,8-cineole, zingiberene, ar-curcumene were also found in both oils. It’s the first report about the volatile oil constituents of endophyte isolates from A. sinensis.
8.Screening of Antitumor and Antimicrobial Activities of the Endophytic Fungi Isolated from Cephalotaxus hainanensis Li
Wen-Jun DAI ; Hao-Fu DAI ; Ping CHEN ; Jiao WU ; Wen-Li MEI ;
Microbiology 2008;0(08):-
Seventy-two endophytic fungi isolated from Cephalotaxus hainanensis Li were screened for antitumor and antimicrobial activities. The results showed that 9 strains presented cytotoxic activities to at least one of the tested tumor cells, five strains exhibited strong antimicrobial activity against Staphylococcus aureus, one strain had inhibitory effect on Phytophthora parasitica. It was evidently that the endophytic fungi of C. hainanensis are potential resources to find valuable bioactive components.
9.Antibacterial components from artificially induced dragon's blood of Dracaena cambodiana.
He-mei JIANG ; Hui WANG ; Jun WANG ; Hao-fu DAI ; Yan-ping LUO ; Wen-li MEI
China Journal of Chinese Materia Medica 2015;40(20):4002-4006
Ten compounds were isolated from the artificially induced dragon's blood of Dracaena cambodiana by various column chromatographies on silica and sephadex LH-20 gel. Based on spectral analysis of NMR and MS, their structures were identified as 3, 4-dihydroxyallylbenzene (1), 3', 4', 5'-trimethoxycinnamylalcohol (2), pinoresinol (3), (2R)-7, 4'-dihydroxy-8-methylflavane (4), (2R)-7,4'-dihydroxy-5-methoxy-8-methylflavane(5),(2S)-7,3'-dihydroxy-4'-methoxy-8-methylflavane(6) ,(2S)-4',7-dihydroxy-6, 8-dimethylflavane(7), 4,2',4'-trihydroxychalcone(8), 4,4'-dihydroxy-2-methoxydihydrochalcon(9) and Cambodianin E (10). Antibacterial activity assay showed that compounds 1, 4 and 10 have inhibitory effect on Fusarium oxysporum f. sp. cuben, Fusarium oxysporum f. sp. niveum, Fusarium oxysporum f. sp. vasinfectum and Ralstonia solanacearum.
Antifungal Agents
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chemistry
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isolation & purification
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pharmacology
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Dracaena
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chemistry
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Drugs, Chinese Herbal
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chemistry
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isolation & purification
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pharmacology
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Fusarium
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drug effects
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Molecular Structure
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Plant Extracts
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chemistry
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isolation & purification
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pharmacology
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Spectrometry, Mass, Electrospray Ionization
10.Polypoid ganglioneuroma combined with juvenile polyp: case report and literature review.
Yan-mei HE ; Wen-yan ZHANG ; Dai-yun CHEN ; Li-li JIANG ; Lei LI ; Wei JIANG
Chinese Journal of Pathology 2006;35(4):250-252
Adolescent
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Colon, Ascending
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pathology
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Colonic Neoplasms
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pathology
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surgery
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Colonic Polyps
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pathology
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surgery
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Diagnosis, Differential
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Follow-Up Studies
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Ganglioneuroma
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pathology
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surgery
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Humans
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Male