Objective To evaluate the efficacy and adverse effects of bleomycin combined with Interleukin-2 for malignant pleural effusions in patients with NSCLC. Methods Malignant pleural effusions were excreted cleanly from indwelling chest tube, bleomycin and Interleukin-2 were injected thorax. Results Total survival rate is 88.5 %, only part patients have fever, chest pain, tetter, nausea. Conclusion Bleomycin combined with Interleukin-2 treat patients with malignant pleural effusion from NSCLC, the efficacy are better, the adverse effects are tolerabe.

Objective:To establish the method of culturing neural stem cells(NSCs) from rat brain. Methods:E14～16 SD embryonic rat brain and postnatal one-day(P1) rat brain were respectively chose to culture neural stem cells,and two digestive methods that simple mechanical digestion and mechanical combined with chemical digestion were used to determine the best conditions in primary culture.Immunocytochemistry and immunofluorescence were used to identify NSCs,neurons and neuroglial cells. Results:Both the primary cells from two different kinds of rat brains expressed nestin antigen and the differentiated cells expressed GFAP and MAP-2 antigen.Conclusion:The key point of primary NSCs culture was obtaining the monoplast suspension.Overdigestion led to cell injury even death. NSCs from embryonic rat brain had better proliferation ability and could form cell clones earlier and more than NSCs from newborn rat brain,but they were easier to be contaminated. Obtaining newborn rat brain and isolation NSCs from newborn rat brain were easier,but NSCs from newborn rat brain had worse proliferation ability.

Objective:To explore the prevalence of fatty liver and its risk factors in civil servants of a certain city.Methods:The 10097 pieces of health examination materials for the civil servants were identified using logistic regression analysis .Results:The morbidity rate of fatty liver was 20.33%.Male prevalence,which was significantly higher,was 5.49 times of female prevalence.With the growth of age and improvement of health care level,the morbidity rate of fatty liver increased gradually.Factors like gender,age,health care level,drink,body mass index(BMI),blood sugar,uric acid,triglyceride,cholesterol and aminotransferase(ALT) were closely related to the onset of fatty liver.The most correlated factors were BMI,triglyceride and gender,with their values being 3.780,2.802 and 2.650 respectively.Conclusion:Civil servants with heavy weight,high triglyceride and male gender are susceptible population of fatty liver.

Objective To explore the relationship between interleukin-8 (IL-8) gene 1633C/T polymorphism and late-onset Alzheimer's disease in order to provide genetic reference data for late-onset Alzheimer's disease.Methods Polymorphism distribution of IL-8 gene 1633C/T in 80 LOAD and 80 normal controls were detected by polymerase chain reaction-restriction fragment length polymorphism(PCRRFLP) analysis.The alleles and gene frequencies type were calculated by the direct counting.Type of gene was proceeded with the Hardy-Weinberg balance estimate.The other statistical analyses were carried out with SPSS13.0 software.The genotype frequencies and allele frequencies were compared with the Chisquare test (x2 test).Results For IL-8 gene1633 C/T,the genotype frequencies (CC,20.0％ ; CT,41.2％ ; TT,38.8％) in the LOAD group were not significantly different from those (CC,11.3％ ; CT,40.0％ ; TT,48.7％) in the control group (P ＞ 0.05).Conclusions IL-8 gene 1633 C/T polymorphism is probably not related to the genetic susceptibility of late-onset Alzheimer's disease.

Objective To explore the effect of air pressure wave on the prevention of lower limb deep venous thrombosis in patients with cerebral stroke and the application value of color Doppler ultrasound (CDUS).Methods 72 stroke patients were randomly divided into experimental group (n =37) and control group (n =35) by random digits table.The control group received routine nursing,conventional and rehabilitation therapy.The experimental group was treated by the air pressure wave for 15 days on the basis of the control group.DVT formation of lower extremities and hemodynamic changes before and after treatment was observed by CDUS,and the results were compared with the X-ray phlebography examination results.Results CDUS displayed the incidence rate of DVT in the experimental group was significantly lower than that in the control group (2.7％ vs 20.0％,x2 =5.449,P ＜0.05).The changes of blood flow velocity and the average reflux time of femoral,popliteal vein in the experimental group had significant differences between before and after treatment (t =8.503,4.254,8.587,2.062,all P ＜ 0.05) ;and in the control group,only popliteal vein blood flow velocity had statistically significant difference (t =6.895,2.116,4.169,all P ＜ 0.05).Compared with the X-ray angiography,8 cases of DVT diagnosed by CDUS were consistent,but 1 case of DVT in the control group was missed diagnosis.Conclusion Air pressure wave can effectively reduce the occurrence of DVT in patients with cerebral stroke.CDUS can accurately diagnose the DVT formation,and can help to understand the hemodynamic change,it has important clinical value for monitoring the preventive effect of DVT.

[Objective]To observe radiographic morphology of femoral head at skeletal maturity of the Salter innominate osteotomy for treatment of patients with Legg-Perthes disease and evaluate sphericity of the femoral head and congruity of the hip.Based on system review of literatures,thes attempted to determine the indications and methods of treatment.[Method]Sixteen patients of Legg-Perthes disease were treated by Salter innominate osteotomy before 1994.The mean age of patients was six years and seven months at operation.Of them,only were 12 patients followed up 10 years and ten months avergely and mean ages of patients were 17 years and 2 months at last follow-up.Growth of plate of femoral head and Y cartilage of acetabulum was closed,indicating skeletal maturity.[Result]According to Stulberg classification of outcome,there were four cases in Stulberg class Ⅰ and six patient in class Ⅱ and one patient in class Ⅲ and one patients in class Ⅳ and no case in class Ⅴ.Lower limb of shortening was averagely 1.5 cm(ranged 0.8~2.0 cm)and the range of motion of hips was almost normal and no abnormal gait was observed.[Conclusion]The Salter innominate osteotomy could keep sphericity of the femoral head and congruity with acetabulum of the hip for children with Legg-Perthes disease and also could be in aid of preventing premature arthritis of hip.

OBJECTIVE:To evaluate the sedative and analgesic effects and safty of fentanyl-propofol and midazolam-propofol by intravenous injection in gastroscopy for aged-patients.METHODS:128aged-patients were randomly divided into3groups:fentanyl-propofol group(groupⅠ),midazolam-propofol group(groupⅡ)and ordinary gastroscopy group(groupⅢ).The effects of sedation and analgesia and the variation of SBP,HR and SDNN were observed.RESULTS:Patients in groupⅠandⅡfelt much less discomfortable than those in groupⅢ(P

Health promotion constitutes an important part of community healthcare service. The authors gave a detailed account of the basic concepts and rationale of health promotion and analyzed its difference from disease prevention, pointing out that factors affecting individuals acceptance of suggested healthy conducts included individual cognition, modifying factor and viability of the conducts. At the same time, the authors, using the health promotion theory, made a detailed analysis of responses to a mammary gland screening among senior and middle aged community women. They argued that the health level of the whole population could be promoted and the mortality rate of malignant tumors lowered through helping individuals in understanding their disease, making use of the influence of external factors like the mass media, and proving convenient and good medical services.

Fracture Fixation ; methods ; Humans ; Mandibular Condyle ; injuries ; Mandibular Fractures ; complications ; therapy ; Maxillary Fractures ; complications ; therapy ; Multiple Trauma ; complications ; mortality

Objective: To investigate the relationship between G505A polymorphism in the encoding region of thrombin-activated fibrinolysis inhibitor (TAFI) and cerebral infarction in Chinese Han population. Methods: A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique was used to detect G505A polymorphism in the encoding region of TAFI in 130 patients with cerebral infarction and 118 healthy individuals. Results: The GG genotype of TAFI G505A accounted for 35.4% (46/130) and GA or AA genotype accounted for 64.6% (84/130) in the cerebral infarction group; and they were 49.2% (58/118) and 50.8% (60/118) respectively in the control group. The difference was statistically significant (P = 0.028). The G and A allele frequencies were 60.4% (157/260) and 39.6% (103/260) respectively in the cerebral infarction group, and they were 69.9% (165/236) and 30.1% (71/236) respectively in the control group. The difference was statistically significant (P = 0.026). Multivariate logistic regression analysis showed that G505A polymorphism in the encoding region of TAFI was an independent risk factor for cerebral infarction (OR = 2.660, 95% CI 1.330-5.317, P = 0.006). Conclusion: The TAFI G505A polymorphism may be one of the risk factors for cerebral infarction.