Adolescent ; Child ; Child, Preschool ; Humans ; Infant ; Infant, Newborn ; International Cooperation ; Pediatrics ; Practice Guidelines as Topic ; standards ; Sepsis ; diagnosis ; physiopathology

In order to increase the yield and quality of the medicinal plant and enhance the competitive power of industry of medicinal plant in our country, this paper analyzed the status, problem and countermeasure of the tissue culture of medicinal plant on large scale. Although the biotechnology is one of the most efficient and promising means in production of medicinal plant, it still has problems such as stability of the material, safety of the transgenic medicinal plant and optimization of cultured condition. Establishing perfect evaluation system according to the characteristic of the medicinal plant is the key measures to assure the sustainable development of the tissue culture of medicinal plant on large scale.
Drug Industry ; methods ; standards ; Medicine, Chinese Traditional ; methods ; standards ; Plants, Genetically Modified ; Plants, Medicinal ; genetics ; growth & development ; Quality Control ; Tissue Culture Techniques ; methods ; standards

Adult ; Eye Injuries ; surgery ; Female ; Humans ; Orbital Fractures ; surgery

Objective To investigate the effect of low-dose hydrocortisone(HC)on hippocampus nuclear factor kappa B((NF-?B)),I?B expression in lipopolysaccharide(LPS)induced septic rats and the role of NF-?B signal transcription pathway in pathogenesis.Methods Fifty-four rats were randomly divided into 3 groups: control group(A group,n=6),model group(B group,n=24),low-dose HC treatment group(C group,n=24).The septic rat model was established by intraperitoneal injection LPS(1 mg/kg),as the intervention by caudal vein injection low-dose HC(6 mg/kg),each of B and C group was subdivied into 2,8,16,24 hours respectively after LPS injection(n=6).At serial time points,the animals in each group were sacrificed,brain tissue samples were harvested to determine NF-?B,I?B expression by immunhistochemistry in hippocampus.Results In B group: NF-?B expression was up regulated compared with A group(P

Nowadays, nanotechnologies have shown wide application foreground in the biomedical field of medicine laboratory tests, drug delivery, gene therapy and bioremediation. However, in recent years, nanomaterials have been labeled poisonous, because of the disputes and misunderstandings of mainstream views on their safety. Besides, for the barriers of technical issues in preparation like: (1) low efficacy (poor PK & PD and low drug loading), (2) high cost (irreproducibility and difficulty in scale up), little of that research has been successfully translated into commercial products. Currently, along with the new theory of "physical damage is the origin of nanotoxicity", biodegradability and biocompatibility of nanomaterials are listed as the basic principle of safe application of nanomaterials. Combining scientific design based on molecular level with precision control of process engineering will provide a new strategy to overcome the core technical challenges. New turning point of translational medicine in nanotechnology may emerge.
Biocompatible Materials ; Nanostructures ; toxicity ; Nanotechnology ; Translational Medical Research

Cutaneous Fistula ; Digestive System Abnormalities ; therapy ; Endoscopy, Gastrointestinal ; methods ; Female ; Humans ; Infant ; Treatment Outcome

To explore the relationship between microecological environment and Paeonia lactiflora the effects of growth years of P. lactillora on rhizosphere bacterial communities were studied by PCR-DGGE and the paeoniflorin content determined by HPLC. Results showed that the soil pH increased with growing years of P. lactillora. In the fourth year, soil pH and enzyme activity reached the highest level, while organic matter content was the lowest. The bacterial diversity had a positive correlation with growing years varied from 3.38 to 3.61. Sequencing results demonstrated that Gammaproteobacteria, llphaproteobacteria, Actinobacteria, Acidobacte- ria and Firmicutes were predominant bacteria kinds in the soil of P. lactillora. Gammaproteobacteria was only detected in the bulk soil, while llphaproteobacteria, Acidobacteria G1l, Actinobacteria were only in the rhizosphere soil and the bacterial community among different growing years were similar except few species. HLPC results showed that paeoniflorin content was 3.26%, 3.30%, 3.36%, 3.41% separately from one to four-year-old P. lactiflora with an upward trend. The correlation analysis indicated that the paeoniflorin content had a positive correlation with soil pH and bacterial diversity, conversely, had a negative correlation with organic matter con- tent. During the growth years the rhizosphere bacterial diversity increased without changes of predominant bacteria and the paeoniflorin content increased without significant differences while its production increased significantly, which was different from the plants showing replanting diseases. This is in line with the farming practice choosing 4-year-old P. lactllora, but not the 1-3 year old one. In addition, the accumulation of paeoniflorin is closely related to soil pH, organic matter content and bacteria diversity, confirming that the geoherblism of P. lactiflora is closely related with microbial environment in the soil.
Bacteria ; classification ; Biodiversity ; Glucosides ; metabolism ; Hydrogen-Ion Concentration ; Monoterpenes ; metabolism ; Paeonia ; growth & development ; metabolism ; microbiology ; Rhizosphere ; Soil ; Soil Microbiology ; Temperature

To explore the mechanism of soil microbial ecology, the differences of fungal diversities in rhizosphere of different provenances of Fritillaria thunbergii were analyzed. The diversities and compositions of rhizo-fungi of the samples were analyzed by using DGGE and 454 pyrosequencing. DGGE results showed the Shannon index of Ninbo provenance planted in Ninbo was the highest one. And its dominant fungi were Ascomycota, Deuteromycota and Zygomycota. Except the same fungi, every provenance planted in Ninbo had its own special ones. From the 454 pyrosequencing, the fungal diversity in Panan producing was the highest which was similar with DGGE result. Among the ten phylum detected in its rhizosoil, Fungi_incertae_sedis, Ascomycota, Mucoromycotina, Basidiomycota and Chytridiomycota almost amounted to 90% of the whole community. The fungal types and amounts in Panan were more than those in Ninbo indicating the differences between producing areas and the advantage of macro genome sequencing. There were 10 phyla, 29 families, 28 genus and 159 species of fungi in Panan provenance, 6 phyla, 20 families, 19 genus, 136 species in Ninbo provenance, 8 phyla, 37 families, 47 genus, 289 species in Nantong provenance and 7 phyla, 25 families, 24 genus, 102 species in the bulk soil. Some genus such as Dothidea, Capnobotryella and Conidiobolus were only existed in Nantong provenance, while Pyrenochae- ta, Glomus and Pseudonectria were only in Panan provenance, which implied these species could grew because F. thunbergii influenced the existence of fungi. Experiments of provenance and producing area of F. thunbergii showed that the fungal diversity of indigenous provenance was higher than that of exotic provenance and each provenance had unique fungal species in the rhizosphere, which indicated that the diversity and structure was shaped cooperatively by the species and soil type. These fungal species are interacted with the soil-rhizhosphere-microbe microecological system, which in turn influence the growth of F. thunbergii.
Ecosystem ; Fritillaria ; genetics ; microbiology ; Fungi ; genetics ; Rhizosphere ; Soil ; Soil Microbiology ; Species Specificity

Objective To evaluate the effect of different nebulization inhalation methods on blood oxygen degree of saturation(SaO2) in infants with asthma.Methods Sixty-two infants with asthma were randomly assigned into 3 groups: air-high-frequency flow stonized inhalation group(n=22),oxygen-high-frequency flow stonized inhalationthe group(n=20) and ultrason jet nebulization group(n=18).Three groups all were gived budesonide suspl.SaO2 was monitored during nubulization.Results There were significant differences of SaO2 levels between oxygen-high-frequency flow stonized inhalationthe group and air-high-frequency flow stonized inhalationthe group,ultrason jet ne-bulization group after 10 min and during inhalation(Pa

Objective To explore the changes of humoral immunity in neonates with hypoxic-ischemic encephalopathy(HIE)and observe the influence of perinatal factors on humoral immune function.Methods Sixty-two neonates with HIE were enrolled and 30 healthy neonates were selected as control group.The percentages of CD19,CD25 of B lymphocyte were detected by Flow Cytometr.The levels of IgG,IgM,IgA and complement C3,C4 were detected by immune rate nephewlometry.The results were analyzed by SPSS 11.5 software.Results 1.The percentages of CD19+(17.93?3.10)%,CD19+CD25+(0.64?0.42)% and the levels of IgM(0.13?0.05)g/L,IgA(0.14?0.07)g/L and complement C3(0.62?0.12)g/L,C4(0.10?0.03)g/L in neonates with HIE were significantly lower than those in the healthy term neonates(Pa0.05).2.There were dignificant diffe-rences of the percentages of CD19+,CD19+CD25+ and the levels of IgM,IgA,complement C3,C4 among the different stages of HIE(Pa