1.Plasma from patients with systemic lupus erythematosus inhibits suppressive activity of mesenchymal stem cells against lupus B lymphocytes.
Ying-Jie NIE ; Li-Mei LUO ; Yan ZHA ; Li SUN ; Ji LUO ; Run-Sang PAN ; Xiao-Bin TIAN
Journal of Southern Medical University 2016;36(8):1090-1093
OBJECTIVETo investigate whether plasma from patients with systemic lupus erythematosus (SLE) inhibits the suppressive effects of mesenchymal stem cells (MSCs) on lupus B lymphocytes.
METHODSMSCs isolated and expanded from the bone marrow of healthy donors were co-cultured with B cells purified from the peripheral blood of SLE patients in the presence of fetal bovine serum or pooled plasma from SLE patients, and the proliferation and maturation of the B lymphocytes were analyzed.
RESULTSs Co-culture with normal MSCs obviously inhibited the proliferation of lupus B cells and suppressed the maturation of B lymphocytes, which showed lowered expressions of CD27 and CD38. The pooled plasma from SLE patients significantly inhibited the suppressive effects of normal MSCs on B cell proliferation and maturation.
CONCLUSIONPlasma from SLE patients negatively modulates the effects of normal MSCs in suppressing lupus B cell proliferation and maturation to affect the therapeutic effect of MSC transplantation for treatment of SLE. Double filtration plasmapheresis may therefore prove beneficial to enhance the therapeutic effects of MSC transplantation for SLE.
B-Lymphocytes ; pathology ; Cell Proliferation ; Coculture Techniques ; Humans ; Lupus Erythematosus, Systemic ; blood ; Lymphocyte Activation ; Mesenchymal Stromal Cells ; cytology ; Plasma
2.Floating-Harbor syndrome: a case report and literature review.
Rong-Min LI ; Ya-Chao LU ; Zhen LI ; Jie-Ying WANG ; Jie CHANG ; Shu-Qin LEI ; Qiao ZENG ; Yan-Mei SANG
Chinese Journal of Contemporary Pediatrics 2019;21(12):1208-1211
Floating-Harbor syndrome (FHS) is an autosomal dominant genetic disease caused by SRCAP mutation. This article reports the clinical features of a boy with FHS. The boy, aged 11 years and 7 months, attended the hospital due to short stature for more than 8 years and had the clinical manifestations of unusual facial features (triangularly shaped face, thin lips and long eyelashes), skeletal dysplasia (curvature finger), expressive language disorder, and retardation of bone age. Genetic detection revealed a novel heterozygous mutation, c.7330 C>T(p.R2444X), in the SRCAP gene. The boy was diagnosed with FHS based on these clinical manifestations and gene detection results. FHS is rare in clinical practice, which may lead to missed diagnosis and misdiagnosis, and gene detection may help with the clinical diagnosis of FHS in children.
Abnormalities, Multiple
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Adenosine Triphosphatases
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Child
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Craniofacial Abnormalities
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Growth Disorders
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Heart Septal Defects, Ventricular
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Humans
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Male
3.Semen parameters in men recovered from COVID-19.
Tong-Hang GUO ; Mei-Ying SANG ; Shun BAI ; Hui MA ; Yang-Yang WAN ; Xiao-Hua JIANG ; Yuan-Wei ZHANG ; Bo XU ; Hong CHEN ; Xue-Ying ZHENG ; Si-Hui LUO ; Xue-Feng XIE ; Chen-Jia GONG ; Jian-Ping WENG ; Qing-Hua SHI
Asian Journal of Andrology 2021;23(5):479-483
The novel coronavirus disease (COVID-19) pandemic is emerging as a global health threat and shows a higher risk for men than women. Thus far, the studies on andrological consequences of COVID-19 are limited. To ascertain the consequences of COVID-19 on sperm parameters after recovery, we recruited 41 reproductive-aged male patients who had recovered from COVID-19, and analyzed their semen parameters and serum sex hormones at a median time of 56 days after hospital discharge. For longitudinal analysis, a second sampling was obtained from 22 of the 41 patients after a median time interval of 29 days from first sampling. Compared with controls who had not suffered from COVID-19, the total sperm count, sperm concentration, and percentages of motile and progressively motile spermatozoa in the patients were significantly lower at first sampling, while sperm vitality and morphology were not affected. The total sperm count, sperm concentration, and number of motile spermatozoa per ejaculate were significantly increased and the percentage of morphologically abnormal sperm was reduced at the second sampling compared with those at first in the 22 patients examined. Though there were higher prolactin and lower progesterone levels in patients at first sampling than those in controls, no significant alterations were detected for any sex hormones examined over time following COVID-19 recovery in the 22 patients. Although it should be interpreted carefully, these findings indicate an adverse but potentially reversible consequence of COVID-19 on sperm quality.
Adult
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Asthenozoospermia/virology*
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COVID-19/physiopathology*
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China
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Gonadal Steroid Hormones/blood*
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Humans
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Male
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Progesterone/blood*
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Prolactin/blood*
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SARS-CoV-2
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Semen/physiology*
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Semen Analysis
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Sperm Count
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Sperm Motility
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Spermatozoa/physiology*
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Time Factors