Objective To obtain stable-expressing cell lines which could constantly express tropic1808 gene and investigate the effect of tropic1808 by detecting the expression level of NF-H in PC12 cells. Methods The recombinant tropic1808 plasmid was constructed and transfected into PC12 cells.PC12 cells stable-expressing tropic1808 were established by G418 selection and confirmed by Western blot.The expression of NF-H in the PC12 stable-expressing tropic1808 was observed by RT-PCR,real time PCR,Western blot analysis and cytohistochemistry methods. Results Western blot confirmed that tropic1808 gene could express stably in PC12 cells.The data of RT-PCR,real time PCR,Western blot and cytohistochemistry all demonstrated that the expression of NF-H was increased in PC12 cells stable-expressing tropic1808.Conclusion PC12 cell lines stable-expressing tropic1808 were obtained and the expression of NF-H up-regulated in the PC12 cells stable-expressing tropic1808 gene.

Cell biology is an important course for all the students of medical school,and this course should be given with different content for the students with different academic background.The major content of cell biology course for the freshmen should be the cellular structure and the sub-cellular structure,and for the senior undergraduates,the content should include molecular basis of the cell function,and for the postgraduates,this course should focused on the molecular mechanisms of the cell function.To teach students in accordance of their background and aptitude is a good way to get a satisfied effect in a course for both teachers and students.

Anticoagulants ; therapeutic use ; Child, Preschool ; Fontan Procedure ; adverse effects ; Heparin ; therapeutic use ; Humans ; Male ; Protein-Losing Enteropathies ; drug therapy ; etiology

Abdominal pain is one of the common symptoms in children with diseases.Acute abdominal pain accounts for some parts of all primary presenting diseases in the emergency department.The clinical features and treatments of fulminant myocarditis,Henoch-Schonlein purpura and diabetic ketoacidosis initially presented with abdominal pain in children were reviewed in the article.

Objective To explore the relationship between interleukin-8 (IL-8) gene 1633C/T polymorphism and late-onset Alzheimer's disease in order to provide genetic reference data for late-onset Alzheimer's disease.Methods Polymorphism distribution of IL-8 gene 1633C/T in 80 LOAD and 80 normal controls were detected by polymerase chain reaction-restriction fragment length polymorphism(PCRRFLP) analysis.The alleles and gene frequencies type were calculated by the direct counting.Type of gene was proceeded with the Hardy-Weinberg balance estimate.The other statistical analyses were carried out with SPSS13.0 software.The genotype frequencies and allele frequencies were compared with the Chisquare test (x2 test).Results For IL-8 gene1633 C/T,the genotype frequencies (CC,20.0％ ; CT,41.2％ ; TT,38.8％) in the LOAD group were not significantly different from those (CC,11.3％ ; CT,40.0％ ; TT,48.7％) in the control group (P ＞ 0.05).Conclusions IL-8 gene 1633 C/T polymorphism is probably not related to the genetic susceptibility of late-onset Alzheimer's disease.

Myocardial infarction (MI) is a major cause of death and disability worldwide.The definition of myocardial infarction has been updated.Released in 2012,Third Universal Definition of Myocardial Infarction of ESC/ACCF/AHA/WHF Expert Consensus,made the clinical application value of troponin clear and suggested to monitor the dynamic changes of cTn in patients with suspected,emphasized the importance of clinical differential diagnosis.In consensus,it also noted that the application of high sensitive troponin can lead to false positives results,and pointeds out that in the assessment of cardiac injury associated with percutaneous or surgical coronary procedures,the rational cut-off value of cTn remains controversival.In addition to acute coronary syndrome,cTn elevations are common in many disease states,and with the improvement of detection sensitivity,low level increase of cTn,will be more.In acute and chronic heart failure,the cTn is elevated,and ACS may be the cause of worsening heart failure,So we should identify whether the cTn elevation is pure from the myocardial injury of heart failure or myocardial necrosis caused by ACS.Nowadays,the increased incidence of myocardial infarction has much to do with the wide application of cTn.Clinical applications of cardiac biomarkers and imaging techniques are flexible as the differences in the diagnostic techniques of myocardial infarction among countries and regions result.

Objective To evaluate the effect of a new schistosomiasis control strategy based on the infection source control in four-lake regions of Hubei Province. Methods The new strategy based on the infection source control included the safe water sup-ply,feces harmless treatment,replacing cattle with machine,captive livestock,chemotherapy,Oncomelania hupensis snail con-trol in susceptible areas,hardening ditches,etc,and the new control strategy was implemented for 7 years in 9 counties(cities or districts)of the four-lake regions. The schistosomiasis situations were investigated before and after the intervention of the new strat-egy. Results The coverage rates of feces harmless toilets(three format toilets),biogas digesters,and safe water supply were 42.00%,23.16% and 93.76%,respectively. The number of ditch hardening was 1 960;the area of eliminating snails was 1 378.42 hm2;the number of persons who received the health education was 3 524 818 accounting for 92.17%of the total popula-tion;the number of person-time of taking active protection measures was 516 636 963. The average annual decline rates of schisto-some infection were 24.42%and 38.38%in residents and cattle,respectively. The endemic villages decreased by 4.77%,the vil-lages with snails decreased by 7.05%,and the villages with snails accounted between 63.19%and 66.21%of the total endemic vil-lages. The area with snails decreased by 90.35%,and the advance schistosomiasis patients decreased by 5.55%,and the fatality rate of advanced schistosomiasis patients decreased by 1.24%. No acute schistosomiasis patients occurred. Type One and Two en-demic(heavy endemic)villages decreased to zero;Type Three endemic(moderate endemic)villages decreased by 10.22%;but Type Four endemic(mild endemic)villages increased by 66.38%;and Type Five endemic(no endemic for 5 years)villages de-creased by 0.22%. The standards of schistosomiasis transmission controlled have achieved on schedule. Conclusion The imple-mentation of the new schistosomiasis control strategy based on the infection source control in four-lake regions of Hubei Province can effectively control schistosomiasis.

20 patients with myofascial pain dysfunction syndrome(MPDS)were were treated by NTI-tss splint only during night for 2 months.After treatment,VAS decreased(P <0.01)and active maximum mouth opening degree increased(P <0.01).NTI-tss splint is ef-fective in the treatment of MPDS.Its side effect needs to be further observed.

Objective To estimate the effective dose during 64-slice CT examination on head,chest and abdomen and determine the conversion coefficient of effective dose and dose length product(DLP).Methods The plain CT scan wag performed in 48 cases on head,50 cases on chest and 45 cages on abdomen using GE Lightspeed 64-slice spiral CT.1The Kv,Ma,scan time,CTDIvol,and DLP were recorded in each examination.Effective dose on patients Was estimated using the SR 250 software.Results The effective dose on head,chest and abdomen was(3.1±0.1),(6.9±0.1)and(8.0±0.1)mSv,and the conversion Coeffieient was 0.0025,0.0191 and 0.0166 mSv·mGy-1·cm-1 respectively.Conclusion CTDIvol、DLP and effective dose were recommended for patient dose control during CT examination.The conversion factor of effective dose and DLP were recommended for radiation risk evaluation during different X-ray examinations.

Objective to compare the calculated effective radiation dose variations in cardiac interventional procedures of coronary angiography(CAG)and percutaneous transluminal coronary angioplasty (PCI)by using the different tissue weighting factors recommended by ICRP Publication 60 with that recommended by ICRP Publication 103.Methods Measurements of organ doses were carried out with an anthropomorphic phantom equipped with thermoluminecent dosimetry.The effective radiation doses were separately calculated using the tissue weighting factors provided by ICRP 60 and provided by ICRP 103,and the results obtained from ICRP 60 were compared with that obtained from ICRP 103.Conversion factors of effective radiation dose to dose area product(DAP)were also calculated according to different publication. Results The variations in the effective dose were 6.88%for CAG and 8.46%for PCI.The effective dose variations in CAG and PCI were 7.25%and 8.76%for female and were 6.51%and 8.17%for male, respectively.The effective conversion factors of DAP were 0.10～0.11 and 0.13～0.14 for CAG and PCI, respectively,according to ICRP 60 and 103.Conclusion The variation of conversion factors of effective dose indicates that in making estimation of effective radiation dose by using the widely accepted conversion factors,the adjustment of organ weighting factors recommended by ICRP 103 should be considered.