Objective To discuss the measurement of bone tumor volume on the basis of three dimensional images segmentation technology. Methods Twenty patients with lacunar bone tumor from Tianjin hospital and Tongji hospital were included in the study from January 2010 to August 2010. There were 11 males and 9 females. Each patient was exposed to spiral CT preoperatively. Then these primitive CT dates were imported into digital orthopedics clinical research platform (SuperImage orthopedics edition 1.1, Cybermed Ltd). The volume and maximum diameter of bone tumor were measured before operation by three-dimensional reconstruction technology. The actual tumor volume was measured during the operation. The tumor volume was also calculated from plain X-rays and CT scans as ellipsoidal or cylindrical depending on the tumor configuration and presence or absence of a soft tissue component. Results The tumor volume was measured to be (14.92±7.34) mm3, (16.65±6.95) mm3 and (34.29±15.70) mm3 using three-dimensional reconstruction technology, intraoperative elevation, and traditional radiograph measurement separately. It was found that there was no difference regarding the outcomes of measurement between three-dimensional reconstruction technology and gross intraoperative measurement. But obvious difference was detected between gross intraoperative measurement and traditional radiograph measurement. Coefficient of correlation between diameter and volume of bone tumor was 0.325 (P=0.162). Conclusion Digital measurement is a precise, efficient,convenient and repeatable method for bone tumor measurement.

Objective To investigate the impact of reduced glutathione(GSH) on the prolifera- tion,oxidative stress and transforming growth factor?1(TGF-?1) expression of human hepatocytes and hepatic stellate cells(HSCs)(LX-2 cell line).Methods Human hepatocytes and HSCs were incubated with various concentrations of GSH(0.5—50 mmol/L or 0.5—10 mmol/L).The effects of GSH on the proliferation of hepatocytes and HSCs were studied by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphennyhera- zolium bromide colorimetric assay.Human hepatocytes and HSCs were co-cultured with GSH and ferric nitrilotriacetic acid,superoxide dismutase (SOD) activity and malondialdehyde (MDA) contents were detected.HSCs were incubated with high(5.0 mmol/L),media(2.5 mmol/L) and low (0.5 mmol/L) concentrations of GSH,the expressions of TGF-?1 mRNA and protein were detected by ELISA and real- time PCR.Results In concentration ranged from 2.5 to 10 mmol/L,the GSH could promote the pro- liferation of hepatocytes but no HSCs,significantly increased the activity of SOD and decrease the con- tents of MDA in hepatocytes and HSCs,and inhibited the expression of TGF-?1 in HSCs.Conclusions GSH can not only promote the proliferation of hepatocytes,but also protect hepatocytes and HSCs from oxidative stress,and inhibit the secretion of TGF-?1 in HSCs.GSH may play a role in hepatocellular protection,antioxidation and anti-fibrosis.

Objective To explore the role of glutamate in inducing cortical neuron damage in newborn rats.Methods The model of damage induced by glutamate was established on cultured cortical neurons in newborn rats with primary cultivation technique.To evaluate the severity of neuron injury, the changes of morphology were observed by inverted microscopy, the cell viability and rate of LDH releasing from neuron were detected by MTT assay and biochemical method,respectively;the rate of neuronal apoptosis was measured by flow cytometer system.Results Under the inverted microscopy, neurons showed obvious toxic damage in glutamate treatment group. Compared with controls,the cell viability significantly decreased (t=4.58 P

Objective：The aim of this study was to investigate the effect of p53 gene alterated expression on human carcinogenesis. Methods： p53 protein expression in 1 364 patients with malignant tumors and 628 benign lesions was detected by immunohistochemistry (LSAB). Results： The frenquency of p53 protein positive was significant higher in malignant tumors [56.67％ (733/1364)] than those in related benign lesions [6.7％ (42/623) (P< 0.001)]; It was revealed the similar situation as compared squamous cell cancer (615), adenocarcinoma (382), and other types of malignancies (367) with their related benign lesions respectively (P< 0.001). Conclusion： Alterated expression of p53 protein (gene multation or protein accumulation) may involve one of the critical events of human carcinogenesis. Detection of p53 overexpression may be a useful biomarkers for assessment of maligant tumors.

Objective To evaluate the feasibility of reconstructing horizontal periodontal bone defects by tissue engineering based on bone marrow stromal cells(BMSCs)as seed cells and enamel matrix proteins(EMPs)as growth factors. Methods Two healthy rhesus monkeys were selected, and BMSCs were isolated from iliac marrow and serial subcultivation was conducted. The cells of induced BMSCs at passage 3 were harvested and mixed with Bio-oss collagen. The models of horizontal periodontal bone defects were established surgically in each buccal side of the posterior teeth, and were divided into four groups (blank control group, material group, cells/material group and cells/material/EMPs group). The histological and Micro-CT observation were carried out 8 weeks later. Results In the blank control group, the defects were filled with fibrous connective tissue. There was newly-formed alveolar bone in the material group. In the cells/material group, periodontal regeneration could be observed, while the newly-formed cementum was irregular and less in quantity. In the cells/material/EMPs group, the amount of newly-formed alveolar bone was larger, and the newly-formed cementum was continuous and regular. Conclusion The tissue engineering technique of BMSCs as seed cells in combination with EMPs induction can significantly promote the regeneration of periodontal tissue.

Adolescent ; Adult ; Antibodies, Viral ; blood ; Female ; Hemagglutination Tests ; Humans ; Influenza A virus ; immunology ; Influenza B virus ; immunology ; Influenza Vaccines ; classification ; immunology ; Influenza, Human ; immunology ; prevention & control ; Male ; Middle Aged ; Safety

OBJECTIVESTo observe the effects of pioglitazone on morphological changes and connective tissue growth factor (CTGF) expression of the transforming growth factor beta (TGF b)-induced rat hepatic stellate cells (HSCs) in vitro, and to investigate the anti-fibrotic mechanism of pioglitazone.

METHODSCultured rat HSCs were divided into a no-treatment control group, a TGF b-treated group, and a TGFb plus different dosage pioglitazone-treated group. The morphological changes of the cultured HSCs were observed. The expression of CTGF was assessed by immunohistochemistry and flow cytometry. The level of collagen type III in the culture supernatant was measured by ELISA.

RESULTSTGFb induced morphological changes, and increased the expressions of CTGF and collagen type III of the HSCs (P less than 0.05). Pioglitazone prevented the TGFb induced morphological changes of the HSCs. The expression of CTGF and the levels of collagen type III in the pioglitazone group were lower than the TGF b-treated group (P less than 0.05). This prevention effect was dose-dependent (P less than 0.05).

CONCLUSIONPioglitazone blocks the excretion of CTGF and collagen type III of cultured HSCs, preventing the development of liver fibrosis.

Animals ; Cells, Cultured ; Collagen Type III ; secretion ; Connective Tissue Growth Factor ; metabolism ; Hepatic Stellate Cells ; drug effects ; metabolism ; Rats ; Thiazolidinediones ; pharmacology ; Transforming Growth Factor beta ; pharmacology

OBJECTIVE: To investigate the protective effect of losartan against on injury induced by ox-LDL in endothelial cells and the relationship with asymmetric dimethylarginine (ADMA). METHODS: Endothelial injury was induced by incubation with ox-LDL 100 mg/L in cultured HUVECs for 24 h, and the levels of ADMA, lactate dehydrogenase (LDH), nitric oxide (NO) and tumor necrosis factor-alpha (TNF-alpha) in the conditioned medium were measured. The activity of dimethylarginine dimethylaminohydrolase (DDAH) of cultured endothelial cells was also determined. RESULTS: Incubation of endothelial cells with ox-LDL 100 mg/L for 24 h induced a marked elevation of the levels of ADMA, LDH and TNF-alpha in the conditioned medium and a significant decrease in the activity of DDAH and the content of NO (P < 0.05). Pretreatment with losartan (10(-8) - 10(-6) mmol/L) significantly inhibited the increased levels of ADMA, LDH and TNF-alpha, attenuated the decreased levels of NO and the decreased activity of DDAH induced by ox-LDL (P < 0.05). CONCLUSION Losartan may preserve ox-LDL-induced endothelial cell injury by increasing the DDAH activity and decreasing the ADMA level.
Amidohydrolases ; metabolism ; Arginine ; analogs & derivatives ; analysis ; Cells, Cultured ; Endothelium, Vascular ; pathology ; Humans ; L-Lactate Dehydrogenase ; analysis ; Lipoproteins, LDL ; adverse effects ; Losartan ; pharmacology ; Nitric Oxide ; analysis ; Protective Agents ; pharmacology ; Umbilical Veins ; cytology

OBJECTIVETo evaluate the clinical efficacy and safety of Chinese drugs for the treatment of children's infectious mononucleosis (CIM).

METHODSSixty CIM patients were assigned into the treated group and the control group, patients in the treated group were administered with Chinese herbal decoction, and those in the control group were treated with intravenous dripping of ganciclovir 10 mg/kg per day, for a treatment course of 14 days.

RESULTSThe total effective rate was 96.0% in the treated group and 97.1% in the control group, showing insignificant difference between groups. The efficacy in the treated group was superior to that in the control group on the fever clearance time (3.0+/-1.5 days vs 4.9+/-3.9 days ) and the disappearance time of cervical lymph node swelling (0.8+/-1.0 score vs 1.5+/-1.2 score), showing statistical significance (all P<0.05). T-cell subsets were markedly improved in both groups after treatment. Adverse reaction occurred in four cases of the control group.

CONCLUSIONUsing Chinese herbs for clearing heat, removing toxin, activating blood circulation, and dissolving stasis is effective and safe for the treatment of CIM. It can effectively improve the clinical symptoms and shows a certain effect on immune regulation.

Antigens, CD ; immunology ; Child ; Herpesvirus 4, Human ; genetics ; Humans ; Infectious Mononucleosis ; drug therapy ; immunology ; Medicine, Chinese Traditional ; Polymerase Chain Reaction

OBJECTIVETo investigate the effects of Hg2+ on voltage-dependent calcium channels and intracellular free calcium in trigeminal ganglion neurons of rats and explore the toxicity mechanism of Hg2+ on these neurons.

METHODSWhole cell patch-clamp technique was used to determine ICa of voltage-dependent calcium channels in trigeminal ganglion neurons of rats. Intracellular free calcium was measured to explore [Ca2+]i dynamic changes from a single cell level by laser scanning confocal microscopy and fluorescence probe techniques.

RESULTS0.01, 0.10, 1.00 and 10.00 micromol/L Hg2+ could reduce voltage-dependent calcium channel currents ICa by (1.80+/-0.32)%, (23.04+/-9.46)%, (58.20+/-7.90)% and (82.00+/-5.77)% in trigeminal ganglion neurons. The inhibiting effects reached their maximum in 5 minutes and could not be reversed significantly during wash with Hg2+-free solution. Also, 0.01, 0.10 and 1.00 micromol/L Hg2+ increased intracellular free calcium concentrations by (2.50+/-0.83)%, (82.81+/-35.36)% and (222.70+/-62.48)% in trigeminal ganglion neurons. Pre-administrated trigeminal ganglion neurons with nifedipine for 10 minutes could decrease the effects and delay the effecting time.

CONCLUSIONThe inhibition of Hg2+ on the voltage-dependent calcium channel currents ICa depends on voltage-dependent calcium channels. And the increase of intracellular free calcium concentration in trigeminal ganglion neurons induced by Hg2+ is related to the release of intracellular stored calcium. However, the relationship between them needs further investigation.

Animals ; Calcium ; metabolism ; Calcium Channels ; drug effects ; metabolism ; physiology ; Cells, Cultured ; Female ; Male ; Mercury ; pharmacology ; Rats ; Rats, Sprague-Dawley ; Trigeminal Ganglion ; cytology ; drug effects ; metabolism ; physiology