4.Application of pulsed field gel electrophoresis on the karyotype analysis of Candida albicans
Medical Journal of Chinese People's Liberation Army 1981;0(04):-
Objective To investigate the karyotype of Candida albicans DNA by pulsed field gel electrophoresis (PFGE), and evaluate the significance of the karyotype used as a method for molecular epidemiology controlling. Methods 14 isolates of C. albicans from various clinical samples were evaluated for molecular relatedness by PFGE. Isolates were identified by MircroScanWalkAway 96 RY-ID. Results The karyotype analysis revealed a total of 9 different genotypes among 14 isolates of C. albicans from 11 patients. Among the isolates, 4 were isolated from difference parts of a patient and had same karyotype, implying that they were from the same isolate and differed to different sub-clones. In another 10 isolates from different patients, 3 had same karyotype, and the karyotypes of the remainder 7 isolates were different. Conclusion PFGE may well reveal the autoploidy and polymorphism of the isolates of C. albicans with different origin, thus may be used as an effective method for molecular epidemiological study.
5.Analysis of surveillance on iodized salt in Baotou during 2008-2010
Zhi-hong, LIU ; Xue-mei, DU ; Dong-mei, ZHAO
Chinese Journal of Endemiology 2011;30(2):179-180
Objective To find out the consumption situation of iodized salt in Baotou, identify problems and take appropriate intervention measures, and to provide scientific basis for further consolidating the results of control measures, strengthening and improving the sustainable elimination of iodine deficiency disorders. Methods Three batches of each quarter, 54 salt samples were sampled in Donghe wholesale division and Qingkun wholesale division in Baotou city salt company during 2008 - 2010; each place of Damaoqi, Baiyun district, and Qingshan district were divided into five sampling areas according to the direction of east, west, south, north, and central position, one school was selected in each district, 30 students aged 8 to 10 from each school were selected, and home salt samples were taken, and salt iodine was tested by direct titration(GB/T 13025.7-1999). Results Qualified rate of wholesale iodized salt was 100%(378/378) during 2008 - 2010, and mean salt iodine was 30.4 mg/kg;qualified rate of household iodized salt was 99.8%(2417/2421 ), and mean salt iodine was 30.4 mg/kg; iodized salt coverage rate was 99.6% (2421/2430) and consumption rate of qualified iodized salt was 99.4% (2417/2430).Conclusions Qualified rate of iodized salt, coverage rate of qualified iodized salt and consumption rate of qualified iodized salt are 90% or more, which has reached the standard of sustainable elimination of iodine deficiency disorders.
8.Autoimmune pancreatitis: report of a case.
Ke SUN ; Hong-tian YAO ; Mei KONG ; Xiao-dong TENG
Chinese Journal of Pathology 2012;41(2):140-141
Autoimmune Diseases
;
diagnosis
;
immunology
;
pathology
;
surgery
;
Humans
;
Immunoglobulin G
;
blood
;
Magnetic Resonance Imaging
;
Male
;
Middle Aged
;
Pancreatectomy
;
Pancreatitis
;
diagnosis
;
immunology
;
pathology
;
surgery
9.Thighbone and cervical bone fraction internal fixing guide pin director
Xiao-Bin DONG ; Zhu-Hong WANG ; Yue-Mei SUN ;
Chinese Medical Equipment Journal 1993;0(05):-
This paper reports a new type of thighbone and cervical bone fraction internal fixing aim di- rector,it has the advantage of entering pin accuratly,simple operating,short time for x-ray irradiating, little suffering to the patient and being economic.
10.Forensic analysis of death caused by fat embolism: A study of 20 autopsy cases.
Lan ZHOU ; Jiao MU ; Hong-Mei DONG ; Ji ZHANG
Journal of Forensic Medicine 2013;29(6):431-433
OBJECTIVE:
To analyze the general and forensic pathological characteristics of death due to fat embolism syndrome (FES) and to provide reference data for forensic identification.
METHODS:
Twenty autopsy cases due to FES were selected from the forensic center of a medical college from 1999 to 2012. The general and forensic pathological characteristics such as the ways and types of injuries, clinical manifestation and the pathological changes were summarized.
RESULTS:
Fat embolism mainly occurred after long bone fracture or a large area of soft tissue injury with the majority of cases being fat embolism of lung and occasional cases being combined embolisms of lung and brain as well. The onset of symptoms appeared shortly after the injury or surgery. Lipid droplets could be observed within small pulmonary vessels and verified by special staining.
CONCLUSION
There are particular characteristics in death due to FES in concern with types of injuries, onset of symptoms and pathological findings. In order to find out the direct evidence of FES, special staining (oil red O staining) can be used in the forensic identification.
Autopsy
;
Cause of Death
;
Death
;
Embolism, Fat/mortality*
;
Forensic Pathology
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Fractures, Bone/complications*
;
Humans
;
Male
;
Middle Aged
;
Pulmonary Embolism/etiology*
;
Soft Tissue Injuries/complications*