1.Application analysis of XT-4000i blood cell analyzer in body fluid cell count
Junjie CHEN ; Rui SHI ; Mei XU ; Fei CHEN
International Journal of Laboratory Medicine 2014;(23):3255-3256
Objective To investigate the application value of the XT-4000i blood cell analyzer in body fluid cell count.Methods 113 cases of body fluid specimen were collected in the hospitalized patients from September 2013 to February 2014.Then RBC and WBC counts in the collected specimens were detected whitin 1 h by XT-4000i blood cell analyzer and the manual detection method, the RBC count values were divided into 3 levels:L1 100~1 000 ×106/L,L2 1 001 ×106 ~100 000 ×106/L and L3 > 100 000 × 106/L;WBC count values were divided into 2 levels:L1 1~50×106/L and L2 >50×106/L.The correlation between the two kinds of test methods was analyzed.Results The results of RBC and WBC counts detected by the XT-4000i blood cell analyzer and the manual method had a higher correlation.The correlation coefficients were 0.931,0.996,0.865,0.942 and 0.988.Conclusion The XT-4000i blood cell analyzer can be applied in clinical fluids cell count.
2.Role of self-made auxiliary device in precise radiotherapy for pelvic tumor
Fei BAI ; Mei SHI ; Jie LI ; Lihua ZHANG ; Lin XU
Chinese Journal of Radiation Oncology 2016;25(6):615-617
Objective To investigate the role of self-made auxiliary device in pelvic tumor radiotherapy with phantom immobilization using Varian cone-beam CT (CBCT).Methods A total of 50 patients with pelvic tumor were enrolled and randomly divided into study group and control group according to the order of enrollment.The patients in the study group were immobilized with thermoplastic phantom and self-made auxiliary device,and those in the control group were immobilized with thermoplastic phantom.CBCT scan and online matching were regularly performed before radiotherapy to obtain the setup errors of the left-right (x),cranial-caudal (y),and anterior-posterior (z) directions.The independent-samples t-test was used for comparison between groups.Results The set-up errors in the x-,y-,and z-directions in the study group were 1.56± 1.00 mm,1.60± 1.29 mm,and 1.36± 1.00 mm,respectively,and those in the control group were 1.76±1.33 mm,2.76±1.69 mm,and 1.92±0.91 mm,respectively (P=0.551,0.009,and 0.043).Conclusions Self-made auxiliary device helps to eliminate the errors in the cranial-caudal direction and solve the problem of involuntary activities of the lower limbs.
3.Recent development of natural and reconstituted lipoprotein based nano drug delivery vehicles.
Ying XU ; Xue-Feng JIN ; Qi-Neng PING ; Hong-Fei LIU ; Mei CHEN ; Xi-Ming XU
Acta Pharmaceutica Sinica 2014;49(1):23-29
Lipoproteins are biological lipids carriers. The natural and reconstituted lipoprotein based drug delivery systems have been extensively developed in recent years. This article reviews the development of natural and reconstituted low-density lipoprotein and high-density lipoprotein based vehicles in the antitumor area.
Animals
;
Antineoplastic Agents
;
administration & dosage
;
chemistry
;
Apolipoproteins B
;
administration & dosage
;
chemistry
;
Drug Carriers
;
administration & dosage
;
chemistry
;
Humans
;
Lipoproteins
;
administration & dosage
;
chemistry
;
Lipoproteins, HDL
;
administration & dosage
;
chemistry
;
Lipoproteins, LDL
;
administration & dosage
;
chemistry
;
Nanoparticles
;
Neoplasms
;
drug therapy
;
Peptides
;
administration & dosage
;
chemistry
;
Pharmaceutical Vehicles
;
chemistry
4.Calreticulin upregulation induced by hypoxic preconditioning relieves oxidative stress injury in rat cardiomyocytes.
Fei-Fei XU ; Xiu-Hua LIU ; Xiao-Mei ZHU
Acta Physiologica Sinica 2008;60(1):29-37
The present study was aimed to investigate whether calreticulin (CRT) was involved in the protective effect of hypoxic preconditioning (HPC) against oxidative stress injury in rat cardiomyocytes. Neonatal cardiomyocytes were prepared from Sprague-Dawley rats aged 24 h, and cultured in DMEM medium containing 10% fetal bovine serum. The cultured cardiomyocytes were randomly divided into 8 groups as follows: (1) hydrogen peroxide stress (H(2)O(2) group); (2) brief hypoxic exposure for 20 min to simulate HPC (HPC group); (3) hypoxic exposure for 20 min followed by normoxic reoxygenation for 24 h before hydrogen peroxide stress (HPC + H(2)O(2) group); (4) SB203580 (a specific inhibitor of p38 MAPK) + HPC + H(2)O(2) group; (5) CRT antisense oligonucleotide transfection (AS group); (6) AS + H(2)O(2) group; (7) AS + HPC + H(2)O(2) group; (8) control group. Morphological observation, lactate dehydrogenase (LDH) leakage and flow cytometry were employed to assess cell apoptosis and necrosis. RT-PCR and Western blot were used to detect CRT expression and activity of p38 MAPK. All experiments were repeated at least four separate times. The results obtained were as follows: (1) HPC relieved cell injury caused by H(2)O(2). Compared with that in H(2)O(2) group, the cell survival rate increased by 18.0% (P<0.05), apoptotic rate and LDH leakage in culture medium decreased by 19.4% and 53.0%, respectively (P<0.05) in HPC + H(2)O(2) group. (2) H(2)O(2) induced CRT over-expression (7.1-fold increase compared with control, P<0.05), while HPC resulted in mild CRT up-regulation (2.4-fold increase compared with control, P<0.05), suggesting that HPC can relieve the over-expression of CRT induced by H(2)O(2). (3) CRT AS transfection weakened the protection of HPC. Compared with that in HPC + H(2)O(2) group, the cell survival rate decreased by 4% (P<0.05), and apoptotic rate and LDH leakage in culture medium increased by 2.6% and 39.0%, respectively (P< 0.05) in AS + HPC + H(2)O(2) group. (4) The protection of HPC and HPC-induced upregulation of CRT were almost eliminated when SB203580 was administered before HPC. These results suggest that HPC up-regulates CRT expression through the p38 MAPK signaling pathway and protects cardiomyocytes from oxidative stress injury.
Animals
;
Apoptosis
;
Calreticulin
;
metabolism
;
Cell Hypoxia
;
Cell Survival
;
Cells, Cultured
;
Hydrogen Peroxide
;
Imidazoles
;
pharmacology
;
Ischemic Preconditioning, Myocardial
;
L-Lactate Dehydrogenase
;
metabolism
;
Myocytes, Cardiac
;
pathology
;
Oxidative Stress
;
Pyridines
;
pharmacology
;
Rats
;
Rats, Sprague-Dawley
;
Signal Transduction
;
Up-Regulation
;
p38 Mitogen-Activated Protein Kinases
;
antagonists & inhibitors
5.Application of forehead dot marking in radiotherapy for brain tumors
Lihua ZHANG ; Fei BAI ; Jie LI ; Lin XU ; Xiaoli LIU ; Mei SHI
Chinese Journal of Radiation Oncology 2016;25(4):385-387
Objective To investigate the role of forehead dot marking in immobilization during radiotherapy for brain tumors.Methods A total of 84 patients with brain tumors were immobilized using the full-head mask from 2013 to 2014;among these patients, 36 received dot marking at the center of the forehead ( group A) , 27 received routine immobilization ( group B) , and 21 received dot marking at one side of the forehead ( group C) .Kilovoltage cone-beam computed tomography ( CBCT) was used to compare the deviation in spatial position.The t-test was used to compare the deviation of the first CBCT in group A with the errors of subsequent CBCT.Results The passing rates of 212 times of CBCT in group A and 158 times of CBCT in group B were 95.2%and 93.8%.The errors in x, y, and z directions in groups A and B were 0.868±0.618 mm vs.1.319±0.935 mm (P=0.005), 1.118±0.692 mm vs.1.149±0.807 mm (P=0.824) , and 0.868±0.790 mm vs.0.936±0.919 mm ( P=0.665) .In group C, the error in the x direction was 1.162±0.866 mm, similar to those in groups A and B ( P=0.070 and 0.430) .In group A, the error in the x direction showed a difference between the first time and the sixth time (0.790±0.656 mm vs.1.280± 0.724 mm, P=0.030) .Conclusions Compared with the routine technique, dot marking at the center of the forehead can improve the accuracy of positioning in the x direction and reduce rotation error, and provides a new method for immobilization in radiotherapy for brain tumors.
6.Inhibitory and dispersive effects of betaine on formation of Staphylococcus aureus biofilm
Fei JIN ; Yi WEN ; Yuqiao XU ; Yaning MEI ; Wenying XIA ; Jue WANG ; Fang NI
Chinese Journal of Clinical Laboratory Science 2017;35(4):261-263
Objective To observe the formation of Staphylococcus aureus biofilm and the inhibitory and dispersive effects of betaine on the biofilm.Methods The inhibitory and dispersive effects of 0.1% betaine on the biofilm from 20 strains of Staphylococcus aureus were examined by crystal violet assay.Results All the 20 strains of Staphylococcus aureus formed biofilm.The biofilm of methicillinsensitive Staphylococcus aureus (MSSA) was formed in 24 hours with peak value of absorbance (A590 nm) (1.99 ± 0.53).The biofilm of methicillin-resistant Staphylococcus atureus(MRSA) was formed in 48 hours with peak value of absorbance(A590 nm) (1.13 ±0.47).After adding betaine,the absorbance(A590 nm) of MSSA biofilm fell down to(1.74 ± 0.61) in 24 hours,while the absorbance(A590 nm) of MRSA biofilm fell down to(0.40 ± 0.12) in 48 hours,which was significantly reduced compared with the controls (t =2.43,5.84,P < 0.05 respectively).When adding betaine after the biofilm formed,the absorbancies (A590 nm) of both MSSA and MRSA showed no significant difference compared with the controls (P > 0.05).Conclusion Betaine could inhibit biofilm formation of Staphylococcus aureus at concentration of 0.1%,but it could not disperse the mature biofilm of Staphylococcus aureus.
7.Granulocytic sarcoma of uterine cervix: report of a case.
Qiu-Yu LIU ; Mei XU ; Yuan-Rui HAO ; Li LI ; Ling-Fei KONG
Chinese Journal of Pathology 2010;39(11):773-774
Adult
;
Carcinoma, Small Cell
;
metabolism
;
pathology
;
Diagnosis, Differential
;
Female
;
Follow-Up Studies
;
Humans
;
Hysterectomy
;
Leukosialin
;
metabolism
;
Lymphoma
;
metabolism
;
pathology
;
Peroxidase
;
metabolism
;
Sarcoma, Myeloid
;
metabolism
;
pathology
;
surgery
;
Uterine Cervical Neoplasms
;
metabolism
;
pathology
;
surgery
;
Uterine Cervicitis
;
metabolism
;
pathology
8.In vitro and in vivo study of fluorescent probe PLGA particles prepared by premix membrane emulsification method.
Tao HU ; Fei-Yan SHI ; Lin-Mei PAN ; Hua-Xu ZHU ; Li-Wei GUO
China Journal of Chinese Materia Medica 2014;39(23):4583-4589
Relatively uniform-sized nanoparticles made of poly (lactic-co-glycolic acid) (PLGA) were prepared by premix membrane emulsification method. After the drug loading property was completed, the dynamic tissue distribution of nanoparticles was recorded. With the average particle size and span as indexes, membrane pore size, number of passing membrane times, membrane pressure, volume ratio of oil-water phase and the concentration of poly(vinyl alcohol) (PVA) in external water phase were investigated by single factor test, the optimum preparation technology of blank PLGA nanlparticles was as following: pore size of SPG membrane was 1 μm, membrane pressure was 1. 15 MPa, the number of passing membrane time was 3, the mass fraction of PVA of 2%, volume ratio of oil-water phase of 1 : 5. Prepared nanoparticles were round with smooth surface, the mean diameter was 332.6 nm, span was 0.010, the confocal laser scanning microscope (CLSM) concluded that fluorescent substance is uniform composizion in PLGA nanoparticle, and the in vivo imaging technology in mice include that the nanoparticles show good liver and spleen targeting property.
Animals
;
Drug Carriers
;
chemistry
;
Drug Delivery Systems
;
instrumentation
;
Emulsions
;
chemistry
;
Fluorescent Dyes
;
chemistry
;
Lactic Acid
;
chemistry
;
Mice
;
Mice, Nude
;
Nanoparticles
;
chemistry
;
Particle Size
;
Polyglycolic Acid
;
chemistry
9.Changes of photopic negative response in patients with exudative age-related macular degeneration and proliferative diabetic retinopathy after intravitreal injections of bevacizumab
Changwa MEI ; Changzheng CHEN ; Yiqiao XING ; Chao FENG ; Zengping LIU ; Guoge HAN ; Fei XU ; Lionfang YI
Ophthalmology in China 2009;18(4):243-246
Objective To evaluate the changes of the waveform of the photopic negative response in flash-electroretinogram, visual acuity and central retinal thickness in the treatment of intravitreal injections of bevacizumub. Design Retrospective self-comparative case series. Partidpants 8 subjects (9 eyes) with exudative age-related macular degeneration and 3 subjects (3eyes) with proliferative diabetic retinopathy. Method Evaluation protocol included examinations of the Early Treatment Diabetic Retinopathy study visual acu-ity, visual field, intraocular pressure, fundus fluorescein angiography, optical coherence tomography and flash-electroretinogram. Intravit-real injections of bevacizumab, 1.25 mg (0.05ml), were given under an operating microscope and aseptic conditions. All the subjects were followed-up one month later. Main outcome Measure The amplitudes of PhNR, visual acuity and central retinal thickness. Re-sult At 1 months, the mean amplitudes of PhNR and mean visual acuity in all cases had no obvious change (n=12, P>0.05).The central retinal thickness reduced obviously (n=12, P<0.05), but it was neither significantly correlated with PhNR (r=0.294, P=0.145) nor with visual acuity(r=-0.358, P=0.073). Conclusion The single intravitreal injection of bevacizumab is showed promising in absorption of in-traretinal edema and subretinal fluid in patients with exudative age-related macular degeneration and proliferative diabetic retinopathy, but the changes of visual function (including PhNR) might need further investigation. (Ophthalmol CHN, 2009, 18: 243-246)
10.Nonhuman Primate Chronic Stroke Model with Middle Cerebral Artery Endovascular Embolism
Qiang WANG ; Tong ZHANG ; Chunyu ZHAO ; Jianmin XU ; Mei WEN ; Zhisheng FEI
Chinese Journal of Rehabilitation Theory and Practice 2012;18(5):401-405
Objective To establish chronic cerebral infarction animal model in nonhuman primate. Methods 10 adult male rhesus monkeyswere embolized the middle cerebral artery (MCA) in contra-lateral of handedness, and divided into M1 segment embolism group (n=3), upper trunk embolism group (n=5), and lower trunk embolism group (n=2). Acute neurological deficit was evaluated with standard neurologicalscale, and the motor function in chronic stage was assessed with a task of retrieving food pill in wells. Results Animals in M1 segmentembolism group all died 38~62 h after surgery. Upper trunk embolism group survived, and MRI showed front parietal cortex infarctioncontra-lateral paralyzed side. All of them paralyzed one side in acute stage, and 4 of them persisted dysfunction in chronic stage, that couldnot finish the task of retrieving food pill in wells; only one completed the task. The lower trunk embolism group paralyzed one side in acutestage, but recovered quickly and completely, that finished the task within 7 d. Conclusion Embolism of MCA upper trunk can cause infarctionof precise and proper size with one side limb dysfunction in the acute stage and long-term dysfunction in most animals, which is feasiblefor treatment and neural plasticity research in recovery.