ObjectiveTo investigate the practical value of non-invasive positive pressure ventilation (NIPPV) in the treatment of acute left heart failure(ALHF).MethodsThe clinical effects and complications of NIPPV performed on 36 cases with severe ALHF on the basis of conventional treatment were evaluated and compared with previous treatment without NIPPV.ResultsOf 36 cases with ALHF treated by NIPPV,30 cases improved,6 cases were altered to receive invasive ventilation or dead.Total effective rate was 83.3%.Compared with 42 cases with severe ALHF treated only by conventional treatment in the past 5 years,with only 26 cases being successfully salvaged and effective rate being 61.9%,significant difference (P

Objective To explore the effect of the self-made pelvic bag on patients with unstable pelvic fracture.Methods Toally 24 patients with unstable pelvic fracture from January 2013 to June 2014 were set as control group and used conventional nursing.Toally 30 patients from July 2014 to December 2015 were set as observation group.The self-made pelvic bag was used in the observation group for pelvic fixation and suspension traction.The degree of pain was compared between the pre-and post-use of the bag.Result The pains after using pelvic bag in the observation group were significantly lower than that of the control group (P＜0.001).Conclusions The self-made pelvic bag can be effective for the pelvic fixation and suspension for the patients with pelvic traction.At the same time it can relieve the pain.

Adolescent ; Adult ; Aged ; Aged, 80 and over ; Chemical and Drug Induced Liver Injury ; etiology ; Child ; Child, Preschool ; Drug Eruptions ; etiology ; Drug Hypersensitivity ; etiology ; Drugs, Chinese Herbal ; adverse effects ; Female ; Humans ; Infant ; Kidney Diseases ; chemically induced ; Male ; Middle Aged

Objective To observe the influence of wind-dispelling therapy on therapeutic effect of blood-cooling and kidney-tonifying herbs for chronic idiopathic thrombocytopenic purpura(CITP),and to investigate its immunoregulatory effect.Methods Forty-two CITP patients were randomized into 2 groups:the treatment group(N=22) received therapy of dispelling wind,cooling blood and tonifying kidney,and the control group(N=20) received therapy of cooling blood and tonifying kidney.Six weeks constituted one treatment course,and the treatment lasted 3 courses.Serum interleukin 2(IL-2),soluble IL-2 receptor(sIL-2R),Fas protein,Fas-L protein,and platelet-associated immunoglobulin G(PAIgG) levels and lymphocyte subtypes were compared before and after treatment.Results In the treatment group,14 patients were clinically cured,5 markedly effective,2 effective,1 ineffective and the total effective rate was 95.5%;in the control group,8 patients were clinically cured,4 markedly effective,3 effective,5 ineffective and the total effective rate was 75.0%.The therapeutic effect in the treatment group was superior to that in the control group(P

[Summary] The genomic DNA was extracted from peripheral blood leukocyte of the patient with thyroid hormone resistance syndrome and 14 members of his family.The exons 1-10 of thyroid hormone receptor β (TRβ) gene were amplified by PCR.The products of PCR were sequenced directly to detect the gene mutation.The results showed that 3 members of this family were confirmed to have the C→T transition mutation at nucleotide 1 303 site within exon 10 of TRβ gene,and the missense mutation results in the substitution of histidine to tyrosine (H435Y).The heterozygous mutation may lead to the occurrence of thyroid hormone resistance syndrome.

Animals ; Cells, Cultured ; Excitatory Postsynaptic Potentials ; Glutamic Acid ; physiology ; Hippocampus ; cytology ; Neurons ; cytology ; physiology ; Patch-Clamp Techniques ; Rats ; Rats, Sprague-Dawley

Long non-coding RNA (lncRNA) is a class of RNA molecules, transcripted by RNA polymeraseⅡ, which consists of more than 200 nucleotides and protein-coding function. Many studies have indicated that lncRNA plays an important role in epigenetics, transcription and post-translational processing. The abnormal expression of lncRNA significantly correlates with occurence,development, and metastasis of renal cell carcinoma (RCC) and prognosis of the patients with RCC. This paper summarizes the advances in the research on lncRNA in RCC to reveal the mechanisms of the disease at the molecular level, in order to provide new methods of prevention, diagnosis, treatment and prognostic assessment of RCC.

Objective To investigate the situation of iodized salt practicing measures, the nutritional status of iodine and the trend of iodine deficiency disorders in Futian District of Shenzhen City, and to evaluate the effectiveness of preventive measures. Methods Every year from 2011 to 2015 in Futian District, five sub-districts were selected according to their position of the east, the west, the south, the north and the center, one school was selected in each sub-district, then sixty students were selected from each school to detect iodine content of their household salt samples;over twenty children (half males and half females) aged 8-10 of those sixty students were selected randomly to measure their thyroid volumes and detect their urinary iodine content;one or two drinking water samples were collected in each sub-district to test iodine content. Thyroid volume was examined by ultrasound method; the salt iodine was tested by the method of direct titration; iodine content in urine and drinking water samples was tested by arsenic cerium catalytic spectrophotometry. Results Between 2011 and 2015, totally one thousand four hundred and eighty-eight edible salt samples were tested. The iodized salt coverage rate, the qualification rate of iodized salt and the consumption rate of qualified iodized salt was 96.1%(1 430/1 488), 99.2%(1 419/1 430), and 95.4%(1 419/1 488), respectively . Four hundred and sixty-one children aged 8-10 were examined;nine children were diagnosed goiter, and the goiter rate was 1.95%. A total of 661 children urine samples were collected. The median urinary iodine was 229.5 μg/L; urinary iodine in 100 - 199 μg/L accounted for 29.8%(197/661), and in 200 - 299 μg/L accounted for 31.0%(205/661). Forty-eight drinking water samples were tested, the iodine content of the drinking water was 10.3 - 22.1 μg/L, and the median water iodine was 16.8 μg/L. Conclusions The iodine deficiency disorders surveillance indicators in Futian District of Shenzhen City all meet the national standards for elimination of the disease. Yet, the level of iodine nutrition needs to be further observed, and both the iodized salt market supervision and the health education should be strengthened.

Objective To investigate the clinical characteristics of purulent meningitis caused by streptococcus pneumoniae.Methods We studied clinical features of 12 children with purulent meningitis caused by streptococcus pneumoniae who were hospitalized from Jan 2007 to Oct 2011 in our hospital.Results Twelve children were penicillin-resistant streptococcus pneumoniae.The ages ranged from 2 months to 10 years.Nine cases(75％) were aged less than 5 years,and 5 cases(41.6％) were aged less than 2 years.All cases were with fever,and with the nervous system symptoms involvement.All cases were with complications:9 cases(75％) with septicemia,8 cases (66.7％) with pneumonia.The white blood cells,blood C-reactive protein,peripheral blood procalcitonin,erythrocyte sedimentation rate,white cells and protein in cerebrospinal fluid were mostly significantly high,sugar in cerebrospinal fluid were low significantly.Eleven cases were eventually treatmented by vancomycin plus 3rd to 4rd generations of cephalosporins antibiotics or other antibiotics.Eight cases were cured,2 cases with serious sequela,and 2 cases died.Conclusion We should attach importance to the meningitis caused by streptococcus pneumoniae.For clinical suspects pneumonia streptococcus meningitis,to given the powerful therapy by vancomycin plus the other antibiotics,as early as possible.

To investigate the weight reduction of hydatid cyst and level of splenocyte subsets in mice immunized with recombinant Bifidobacteria bifidum (Bb) Bb-Eg95-EgA31 vaccine of Echinococcus granulosus (Eg) and challenged with Eg protoscoleces,BALB/c mice were immunized with the vaccine subcutaneously,intramuscularly,intranasally or orally.Then the mice were challenged with 50 Eg protoscoleces on 8~(th) week after vaccination and killed on 25~(th) week after infection.The weight of hydatid cyst was measured and the reduction of the weight was calculated.The spleens were collected for splenocytes preparation.CD_4~+ and CD_8~+ T cells were determined by flow cytometry(FCM),and the blank vector,Bb or MRS were set as control.The hydatid cyst weight was reduced by 45.33%,41.33%,70.67% and 62.67% respectively for the 4 immunization groups.Number of CD_4~+ and CD_8~+ subsets in the immunization groups was more than that in the control group.CD_4~+ and the ratio of CD_4~+/CD_8~+ in the intranasal or oral immunization group was much higher than that in the subcutaneous or intramuscular immunization group.It's concluded that CD_4~+ and CD_8~+T cells may play an important role in the protection induced by recombinant Eg95-EgA31 vaccine of Eg.In addition,intranasal and oral vaccinations could be the better ways for immunization.