Humans ; Humidity ; adverse effects ; standards ; Incubators, Infant ; Infant, Newborn ; Intensive Care Units, Neonatal ; Water Supply ; standards

Child ; Hemofiltration ; Humans ; Male ; Paraquat ; poisoning ; Poisoning ; therapy ; Treatment Outcome

To evaluate the items of critical values and alert limits of the test results , to optimize the critical values report procedure , to modify the laboratory information system ( LIS ) and the hospital information system ( HIS ) , the critical values monitoring platform was designed .Through the monitoring platform,the critical value report rate and critical value report timely rate could be calculated , so reduce medical risks and improve the level of hospital management .

Effect of S83143, a praziquantel derivative, and its phosphate salt S85009, sulfate salt S85010 and hydrochloride salt S85021 on different developmental stages of Schistosoma japonicum in mice were ob- served. When infected mice were treated with S83143 suspension at a single oral dose of 400 mg/kg or with 3 different salts of S83143 at a single intramuscular dose of 100 mg' ( S83143 base )/kg, the worm reduction rate was 69~87% in 2 h old schistosomula and 47~96% in 32~33 d old adult worms, respectively, but had no effect on 3 ~14 d schistosomula. The results showed that the susceptibility of different developmental stages of schistosome to S83143 was similar to that of praziquantel.The acute LD50 of ig S83143 suspension was more than 10g/kg, while ED50 was 352?65 mg/kg. With im S85010 solution, the LD50 and ED50 were 176?14 and 66?10 mg/kg respectively. Therefore, the therapeutic index of S83143 was over 28, which was higher than 2.67 of S8S010.

Recently,studies have demonstrated that several signaling pathways including Wnt,Notch,and Hedgehog which are involved in the regulation of the stem cells are abnormally activated in lung cancer.They are closely associated with some properties of the lung cancer stem cells,such as high tumorigenic,high metastasis,drug resistance and so on.In addition,several studies have shown that the population of the lung cancer stem cells,which are resistant to radiotherapy and chemotherapy significantly,highly express drug resistance proteins.Therefore,how to target lung cancer stem cells and ultimately cure the disease is becoming a hotspot in the cancer targeted therapy.

Objective To evaluate the effect of a new schistosomiasis control strategy based on the infection source control in four-lake regions of Hubei Province. Methods The new strategy based on the infection source control included the safe water sup-ply,feces harmless treatment,replacing cattle with machine,captive livestock,chemotherapy,Oncomelania hupensis snail con-trol in susceptible areas,hardening ditches,etc,and the new control strategy was implemented for 7 years in 9 counties(cities or districts)of the four-lake regions. The schistosomiasis situations were investigated before and after the intervention of the new strat-egy. Results The coverage rates of feces harmless toilets(three format toilets),biogas digesters,and safe water supply were 42.00%,23.16% and 93.76%,respectively. The number of ditch hardening was 1 960;the area of eliminating snails was 1 378.42 hm2;the number of persons who received the health education was 3 524 818 accounting for 92.17%of the total popula-tion;the number of person-time of taking active protection measures was 516 636 963. The average annual decline rates of schisto-some infection were 24.42%and 38.38%in residents and cattle,respectively. The endemic villages decreased by 4.77%,the vil-lages with snails decreased by 7.05%,and the villages with snails accounted between 63.19%and 66.21%of the total endemic vil-lages. The area with snails decreased by 90.35%,and the advance schistosomiasis patients decreased by 5.55%,and the fatality rate of advanced schistosomiasis patients decreased by 1.24%. No acute schistosomiasis patients occurred. Type One and Two en-demic(heavy endemic)villages decreased to zero;Type Three endemic(moderate endemic)villages decreased by 10.22%;but Type Four endemic(mild endemic)villages increased by 66.38%;and Type Five endemic(no endemic for 5 years)villages de-creased by 0.22%. The standards of schistosomiasis transmission controlled have achieved on schedule. Conclusion The imple-mentation of the new schistosomiasis control strategy based on the infection source control in four-lake regions of Hubei Province can effectively control schistosomiasis.

Objective To explore the distribution of high-arsenic water in Shaanxi Province in order to provide theoretical basis for the control of arsenism.Methods The contents of arsenic of 4042 water sampled from 1898 nature villages in 12 counties were assessed using semi-quantitative reagent-knit,and the water samples of Shanyang County,the targeted area,was quantified using quantitative atomic fluorescent speemetry.Results The contents of arsenic of 6 water samples in 2 villages exceeded 0.05 mg/L,accounting for 0.15%(6/4042),were 0.46,0.53,0.63,0.69,0.74,1.53 mg/L,respestively,and located in Shanyang County.A crowd of 1146 exposed to hish asenic water.among whom 555 were children.Conclusions Arsenic in drink water in Shanyang County in Shaanxi Province is higher than the standard,to which a large number of people are exposed,water-improving and defluoridotion must be carried out as soon as possible,reduces harm of high arsenic.

OBJECTIVETo explore the mechanism of natural moxibustion on regulating immune imbalance in asthma rats.

METHODSSeventy SD male rats were divided into a normal group, a placebo group, a dexamethasone group, a big-cake for long-course moxibustion group, a big-cake for short-course moxibustion group, a small-cake for long-course moxibustion group and a small-cake for short-course moxibustion group, ten rats in each one. The rat model of asthma was established by egg albumen sensitization and stimulation in all the groups except the normal group. The natural moxibustion was used in all moxibustion groups, in which big cake of 1 cmX 1 cm size was used in the big-cake groups and small cake of 0.5 cmX 0. 5 cm size was used in the small cake groups. According to relevant acupoints, the natural moxibustion was performed, 5 h per time, once a day. Four times of treatment was considered one course, and three courses were required in the long-course groups and one course was required in the short-course groups. Intraperitoneal injection of dexamethasone was applied in the dexamethasone group, which had the same course as long-course moxibustion group. After the treatment, changes of EOS in peripheral blood of asthma rats were observed; enzyme linked immunosorbent assay (ELISA) was applied to test the contents of IgE, IL-4 and IFN-gamma in the lung tissue; real-time Q-PCR method was adopted to measure the expression level of transcription factor T-bet and GATA binding protein 3 (GATA-3) in the lung tissue.

RESULTSCompared with the normal group, the EOS in whole blood as well as IL-4 and IgE in plasma were all increased in the placebo group (all P< 0. 01), IFN-gamma in plasma was obviously decreased (P<0. 01); while the levels of EOS, IgE and IL-4 were significantly reduced (all P<0. 01), the content of IFN-gamma was increased (P<0. 01) in all moxibustion groups and dexametnasone group. Compared with the normal group, the expression of T-bet mRNA in the placebo group was significantly reduced (P<0. 01). Each treatment group could significantly increase the expression of T-bet mRNA and reduce that of GATA-3 mRNA (P<0. 01). Compared with the short-course moxibustion groups, the expression of T-bet mRNA was obviously increased in the long-course moxibustion group and dexamethasone group (both P<0.01), and that of GATA-3 mRNA was reduced (P<0.01). There was no significant difference between long-course moxibustion group and dexamethasone group (P> 0. 05), and also no significant difference could be seen between big-cake moxibustion group and small-cake moxibustion group (P>0. 05).

CONCLUSIONThe natural moxibustion could obviously reduce airway inflammation in asthma rats. With time passing, the efficacy is enhanced, indicating evident timeliness, which has no apparent relationship with the size of moxibustion cake.

Animals ; Asthma ; genetics ; metabolism ; therapy ; Cytokines ; genetics ; metabolism ; Humans ; Male ; Moxibustion ; Rats ; Rats, Sprague-Dawley ; Transcription Factors ; genetics ; metabolism

Female ; Humans ; Male ; Sepsis ; complications ; Wernicke Encephalopathy ; diagnosis

Objective To explore the change of occludin mRNA in the lung tissue under hyperoxia induced lung injury condition and their regulation of platelet-derived growth factor B(PDGFB).Methods Three hundred and twenty newborn rats were divided into 4 groups accor-ding to different oxygen concentrations(FiO2):experimental group 1(FiO2=800 mL/L),experimental group 2 (FiO2=600 mL/L),experimental group 3(FiO2=400 mL/L) and room-air control group(FiO2=210 mL/L).Rats were killed at 1st,3rd,5th,7th and 14th day respectively during the experiment,the expression of occludin was examined by reverse transcription polymerase chain reaction(RT-PCR) method.The expression of PDGFB in the lung tissue was also observed by immunohistochemistry.SPSS 11.5 software was used to analyze the data.Results The expressions of PDGFB in the lung tissue in experimental group 1 and experimental group 2 were lower than those of the control group at 1th day(Pa