Adolescent ; Adult ; Child ; Child, Preschool ; Heart Defects, Congenital ; surgery ; Humans ; Young Adult

Biomarkers ; analysis ; Environmental Monitoring ; methods ; Humans ; Occupational Exposure ; analysis ; Xylenes ; analysis

Adult ; Ascorbic Acid ; therapeutic use ; Drug Therapy, Combination ; Drugs, Chinese Herbal ; administration & dosage ; therapeutic use ; Female ; Humans ; Infusions, Intravenous ; Male ; Myocarditis ; drug therapy ; virology ; Phytotherapy ; Virus Diseases ; drug therapy

Animals ; Autoantigens ; genetics ; metabolism ; physiology ; Centromere Protein A ; Chromosomal Instability ; Chromosomal Proteins, Non-Histone ; genetics ; metabolism ; physiology ; Gene Expression Regulation, Neoplastic ; Humans ; Kinetochores ; metabolism ; Mitosis ; physiology ; Rectal Neoplasms ; genetics ; metabolism ; pathology ; Spindle Apparatus ; metabolism

Acquired Immunodeficiency Syndrome ; diagnosis ; therapy ; Female ; Humans ; Male ; Syphilis ; diagnosis ; therapy

Adolescent ; Asthma ; blood ; drug therapy ; Case-Control Studies ; Child ; Child, Preschool ; Female ; Humans ; Interleukin-17 ; blood ; Male

Objective: To investigate the diterpenoid alkaloids from Aconitum episcopale. Methods: The obtained compounds were isolated by different chromatographic methods and their structures were identified by physicochemical properties and spectral data. Results: Ten diterpenoid alkaloids were isolated and identified as talatizamine (1), chasmanine (2), crassicauline A (3), foresaconitine (4), acoforestinine (5), yunaconitine (6), 3-deoxy-8-deacetyl-yunaeonitin (7), leucanthumsine D (8), pengshenine B (9), and macrorhynine B (10). Conclusion: Compounds 2-5 and 7-10 are isolated from this plant for the first time.

OBJECTIVE: To study the phenolic constituents from Caesalpinia decapetala (Roth) Alston. METHODS: The ethanol crude extract of C. decapetala was fractionalized by using petroleum ether and chloroform. The chloroform part was isolated by a series of chromatography methods, and the structures of purified compounds were elucidated by their physicochemical properties and spectroscopic data. RESULTS: Eleven phenolic compounds were isolated and identified as methyl 2,3,5-trihydroxybenzoate (1), protocatechuic acid methyl ester (2), N-trans-feruloyl tyramine (3), trichostachine (4), cinnamylpiperidine (5), gallic acid (6), methyl 3,4,5-trihydroxybenzoate (7), ethyl 3,4,5-trihydroxybenzoate (8), resveratrol(9), 3,4,3',5'-tetrahydroxydistyrene (10), and protosappanin A (11). CONCLUSION: Compounds 1-5 are isolated from this plant for the first time.

Objective: To investigate the expressions of hypoxia-inducible factor-1α (HIF-1α), glucose transporter 1 (GLUT1), multidrug resistance associated protein (MRP1), and glutathione-S-transferases (GST-π) in non-small cell lung cancer (NSCLC) tissues and their relationship with the biological features of lung cancer. Methods: The expressions of HIF-1α, GLUT1, MRP1, and GST-π mRNA in 36 samples of NSCLC tissues and 12 samples of corresponding adjacent tissues were detected by RT-PCR and semiquantitative agarose gel electrophoresis. Their expression in various TNM staging cancer tissues was analyzed by SAS8.0 statistic software. Results: The expressions of the four genes significantly increased in NSCLC tissues than in the corresponding adjacent tissues (P < 0.01). The expressions of the four genes were significantly higher in the group with lymph node and distant metastasis than that in the groups without lymph node and distant metastasis (P < 0.01 or P < 0.05). The expressions of HIF-1α and GLUT1 mRNA were significantly increased in T3 and T4 staging cancer tissues than that in T1 and T2 staging cancer tissues (P < 0.01 or P < 0.05). The expressions of MRP1 and GST-π mRNA had no significant difference between NSCLC tissues at T1, T2, T3 and T4 staging(P >0.05). Conclusion: HIF-1α, GLUT1, MRP1, and GST-π were over-expressed in NSCLC tissues, which were associated with lymph node and distant metastasis. Expressions of HIF-1α and GLUT1 were elevated with the increase in the TNM staging but expressions of MRP1 and GST-π did not correlate with TNM staging. HIF-1α mRNA expression was positively related with the expression of GLUT1 mRNA (P < 0.01). The expression of HIF-1α mRNA was significantly related with the expression of MRP1 and GST-π mRNA in linear dependency (P <0.05). The expression of GLUT1 mRNA was not significantly related with the expression of MRP1 and GST-π mRNA (P > 0.05). The over-expressions of the four genes may be useful markers indicating the carcinogenesis, Progression, and deterioration of NSCLC.

Objective To study the clinical significance of molecular markers and routine coagulation tests in patients with cerebral infarction and to set up a serial programs of laboratory diagnosis,monitoring and treatment of cerebral infarction.Methods Prothrombin fragment 1+2(F1+2),thrombin antithrombin III complex(TAT), D-dimer(D-D),Von willebrand Factor(vWF),antithrombin(AT), protein C(PC),prothrombin time(PT),activated partial thromboplastin time(APTT) and thrombin time (TT) were determined in 90 patients with cerebral infarction and 60 normal control subjects.Results The levels of F1+2,TAT,D-D,vWF were significantly higher in patients with cerebral infarction than that in control subjects. But the levels of PC,AT,PT,APTT,TT in patients with cerebral infarction were no different from that in control subjects.Conclusion There were hypercoagulable states in patients with cerebral infarction.The activity of prothrombin is higher, thrombin is generated more, the activity of fibrinolysis is higher too, but anticoagulation system is not sufficiently activated .Meanwhile, perhaps endothelial lesion would be the main role of coagulation system activating and pathogenesis.Molecular markers such as F1+2,TAT,D-D,VWF can be as diagnositic signs but routine coagulable tests can not display the hypercoagulable states in patients with cerebral infarction.