Objective To explore the effects of fosinopril and metoprolol on cardiac remodeling and the plasma levels of angiotensin II(AngⅡ),endothelin-1(ET-1) and nitric oxide(NO) in patients with congestive heart failure(CHF).Methods A total of 80 patients with CHF were divided into 3 groups,fosinopril(20 mg/d),metoprolol(from 6.25 mg,twice a day to 100 mg,twice a day or the highest tolerant dose) and combination groups(as in single use),and all were treated for 12 months.Electrocardiography including ejection fraction(EF)and left ventricular end diastolic diameter(LVEDD)was carried out,and plasma levels of the 3 neurohormones were detected with conventional methods at admission and 12 months after the treatment.(Results Plasma) levels of AngⅡ,ET-1 and NO were decreased significantly in all groups.No significant difference was seen between fosinopril and metoprolol groups,but the plasma levels was decreased remarkably(P

Objective To prospectively assess the association between cardiac resynchronization therapy (CRT )and left atrial volume .Methods Fourteen patients with chronic heart failure ,sinus rhythm and left bundle branch block were enrolled in this study and prepared for CRT implantation .Clinical and echocardiographic evaluations were performed before CRT implantation and 6 months later .Followings were parameters of the left atrial and left ventricular volume:left ventricular end-systolic volume index (LVESVi)and end-diastolic volume index(LVEDVi) ,maximal LAV index(iLAVmax) ,etc ,LAV index before atrial systole(iL-AVpre)and minimal LAV(iLAVmin) .CRT responders were defined as those whose reduction rates were more than 10% in iL-VESV in 6-month follow-up .Results Responders(71 .4% )and non-responders(28 .6% )had similar baseline ,clinical characteristics and pre-implantation LV volumes .However ,iLAVmax in the responders was remarkably lower than that in non-responders .CRT therapy significantly reduced LVESVi(P<0 .01) ,LVEDVi(P<0 .001) ,iLAVmax(P=0 .004) ,iLAVpre(P=0 .003)and iLAVpost (P<0 .01) ,but when compared with the non-responders ,iLAVmax was much lower in responders .Correlation analysis showed that there was a significant correlation of LVEDVi with iLAVmax ,iLAVpost(P<0 .05) .Conclusion CRT therapy can significant-ly improve iLAVpre and iLAVmax may be a predictor of LV reverse remodeling .

In order to complete the seven-year microbiology teaching better,we used the theme discussion of PBL teaching in our teaching activities,i.e.by setting a certain scenario and giving some background information before the students'group discussion. The practice proved that the theme discussion of PBL teaching method in theoretical teaching and learning changed the traditional case-based discussion chiefly by teachers; as a result,the relative immobilization of the teaching mode became more flexible. These methods of teaching and learning not only stimulated the students'initiative and enthusiasm,but also helped them to think critically. Our assessment of PBL teaching and learning showed that the themes discussion of PBL in medical microbiology achieved better results.

Objective To evaluate the accuracy of lung volume in pneumothorax by CT.Methods 1.17 artificial lung models were scanned by SHIMADZU SCT-7000 TH whole body scanner system and their volumes were determined by using the volume-calculated function of CT.2.The actual volume of the models were equal to the volume of water injection by the models.Results The actual and calculated volumes of lung were 2893.47?29.22 ml and 2894.32?28.47 ml respectively.A good correlation(?=0.9860,?

Biomarkers ; analysis ; Environmental Monitoring ; methods ; Humans ; Occupational Exposure ; analysis ; Xylenes ; analysis

Objective To study the possibility of targeting endothelial cells with the 131I labeled monoclonal antibody (mAb) for the treatment of hepatocarcinoma. Methods An animal model of human hepatocarcinoma in nude mice was reproduced, and tumor inhibiting activity of the 131I labeled mAb was tested. 30 nude mice with hepatocarcinoma were randomly divided into 3 groups: in group A the mice were treated with mAb 200?g/200?l twice a week; group B mice were treated with the 131I labeled mAb 200?g/200?l twice a week; control group mice were given normal saline in equal volume. Tumor growth in mice was observed. After the mice were sacrificed, the tumor was histologically examined and the intra-tumor microvessel density (TMVD) recorded. Results The tumor growth inhibition effect in mice treated with mAb was 74.55%. This effect was enhanced when treated with the 131I labeled mAb, as evidenced by an increase of tumor growth inhibition rate to 86.36%. Pathologically, Massive necrosis of tumor cells around the degenerated vessels was observed in the mAb treated mice. TMVD was significantly lower in the mAb treated mice than that in the untreated mice (P

Neovascular age-related macular degeneration (AMD) is the main cause of vision loss in AMD patient.Now,the application of anti-vascular endothelial growth factor(VEGF) has become the preferred therapy for the treatment of neovascular AMD,and it has been proved to improve the visual acuity and reduce the blindness rate.But the formation of choroidal neovascularization (CNV) in AMD is a comprehensive and integrated pathological process.It is difficult to cure the CNV with only anti-VEGF drug for all the neovascular AMD patients.Therefore,the targeting drugs toward other signal pathway associated with CNV are being in different phases of clinical trials.Resent years,with the development of science and technology,small interference RNA(siRNA) and genome technology has been applied to treat neovascular AMD and show a potential prospect,and gene therapy and stem cell therapy are the most remarkable and predominant ways for AMD.Doubtlessly,these therapies offer novel choice for the management of neovascular AMD.

Objective: To observe the curative effect of image guided radiation therapy on orbital inflammatory pseudotumors. Methods:28 cases of orbital inflammatory pseudotumors in our hospital from September 2009 to September 2012 were treated with image guided radiation therapy (IGRT) with a single dose of 2GY/F, 1F/d and a total dose of 20GY/10F. Radiation therapy was completed in 2 weeks at 10 times. Results: 23 patients (82%) were completely cured and 3 patients (11%) were partly cured with the total efficiency of 93%. Conclusion:After IGRT treatment, the symptoms in the patients like eye pain, exophthalmos, eye movement disorders and other symptoms were disappeared. IGRT could relieve pain, improve the cure rate and reduce recurrence.

Objective To establish a method for determination of chrysophanol,emodin and rhein in Huangzhi capsules.Method Chrysophanol,emodin and rhein in Huangzhi capsules was determined by TLC-scanning with a mixture of n-hexane-ethylacetate-formic acid(30∶10∶0.5) as the developing system.The detection wavelength was 435 nm and 610 nm.Results The calibraction curve of chrysophanol,emodin and rhein was linear in the range of 0.014 65～0.073 25,0.013 35～0.066 75,0.012～0.072 ?g respectively.Conclusion The method was simple,accurate with good repeatability,and suitable for the content determination of chrysophanol,emodin and rhein in Huangzhi capsules.

Objective To prepare and characterize nanoparticles in-loaded one-way release-controlled chitosan membrane,and to explore the release-controlled rule of the film in vitro.Methods The chitosan nanoparticles were prepared by inverse crosslinking-emulsion method.The one-way release-controlled membrane was prepared by a casting method.Transmission electron microscope (TEM) was used to evaluate the morphological properties and particle size analyzer was used to analyze particle size distribution.The morphology of the membrane was inspected through scanning electron microscope (SEM).MTT assay was applied to determine the biological safety of chitosan nanoparticles.The distribution of the nanoparticles was observed by fluorescence microscope.The in vitro release studies were adopted to evaluate the release-controlled rule.Results The four kinds of nanoparticles had spherical shapes and uniform particle size.The size of the hyaluronic acid-coated chitosan nanoparticle was (255.40±39.10) nm.Hyaluronic acid-coated chitosan nanoparticles showed the best property of sustained release and biocompatibility.The membrane had a loose inner layer and a dense outer layer,and the distribution of the nanoparticles was uniform in the inner layer of the membrane.The release of protein from membrane was unidirectional and the membrane displayed good controlled release property.Conclusions The nanoparticles in-loaded one-way release-controlled chitosan membrane presents good one-way sustained release performance.It is potentially useful in delivery system of growth factors.