Objective To detect multidrug resistance gene(mdr1) transcription in peripheral blood lymphocyte(PBL) before and after chemotherapy and to determine its correlation with chemotherapeutic effect.Methods The peripheral blood sample was taken from each lung cancer patients(n=32) before chemotherapy and at 3rd week after chemotherapy and subjected to fluorescent quantitative real-time RT-PCR.Simultaneously the tumor size was determined by chest X-ray or CT.Results The mdr1 mRNA level in PBL after chemotherapy increased significantly as compared with that before chemotherapy [(4.316 6?0.923 1) vs(3.881 8?0.828 0),t=-4.207,P=0.000].There was an negative correlation between mdr1 mRNA level and the tumor size(r=-0.389,P=0.028).The level of mdr1 transcription in SCLC patients was significantly less than that of NSCLC patients before chemotherapy [(2.831 4?0.398 4) vs(4.231 9?0.603 4),t=6.101,P=0.000].Conclusion In terms of an anticancer drug resistant mechanism,there could be a difference between NSCLC and SCLC.Longitudinal study of mdr1 level in PBL is valuable for evaluating the treatment(response.)

Objective:In order to develop a new standard of curative effect,this study was designed to track the number of CTCs in patients with lung cancer during chemotherapy.Methods:Samples of peripheral blood was taken from each lung cancer patients(n=32) on the day before chemotherapy as well as the third week after the chemotherapy cycle.The samples were subjected to real-time fqRT-PCR.Meanwhile the tumour size was determined by chest X-ray or computed tomograghy. Results:Compared to that of pre-chemotherapy,the expression level of CK19 in the patients significantly declined after chemotherapy(t=4.659,P=0.000 1).The level of CK19 mRNA in patients with SCLC was higher than that of patients with NSCLC(t=1.944,P=0.041).The change of CK19 mRNA correlated well with the type during the treatment.Relatively the shift of SCLC is more obvious(t=6.073,P=0.000 1).Conclusions:The real-time fluorescent quantitative-PCR approach is useful for measuring the relative number of CTCs in a patients' peripheral blood to monitor the effectiveness of treatment,and for designing more comprehensive and reasonable therapeutic regimes at earlier dates for patients.

OBJECTIVE:To investigate the characteristics and regular pattern of the adverse drug reactions (ADR) occurred in our hospital.METHODS:A total of 174 ADR cases reported by our hospital from May 2006 to June 2008 were categorized and analyzed statistically.RESULTS:The ADR were induced by 16 categories(83 kinds) of drugs,predominantly by the antimicrobial agents(12 kinds,60 cases) and Chinese drugs preparations(17 kinds,66 cases).The ADR of 57 cases(26.89%) manifested chiefly as lesions of skin and its appendages,followed by gastrointestinal lesion(39 cases,18.40%).CONCLUSION:Timely ADR reporting is conducive to ADR incidence reduction and clinical rational drug use.

Objective:To evaluate the real-time fluorescent quantitative-polymerase chain reaction approach(fqRT-PCR) of cytokeratin 19(CK19)targets for its feasibility to estimate the number of circulating tumor cells(CTCs)in the patients with lung carcinoma during radiotherapy and to correlate our observation to clinical findings.Methods:Samples of 5ml peripheral blood were taken from each lung cancer patients(n=30)both before and after the radiotherapy course.Meanwhile tumour size was determined by chest X-ray or computed tomography.The blood samples were subjected to real time RT-PCR assay.All patients with lung cancer were treated with primary definitive and mediastinal radiotherapy.Results:Compared to with that before the-treatment,the value of CK19 mRNA in PB after therapy decreased dramaticall(y5.093 2?1.062 8 vs 4.249 3 ?0.832 3,t=3.192,P=0.007).The change of CK19 mRNA level before and after radiotherapy was closely related to the typ(esquamous cell cancer/adenocarcinoma vs.small cell cancer,0.538 9?0.903 0 vs 1.682 6?0.946 7,t=2.146 5,P=0.051).Meanwhile,there was a close link between the grade(Well/Mod vs Poor)and the change of CK19 mRNA(0.502 4 vs 1.527 1,t=2.017,P=0.065).The change of CK19 mRNA level in peripheral blood(PB)was related to variation of tumour burden during radiotherapy(r=0.057 5,P=0.025).Of the 30 cases studied,24 cases were positive before radiotherapy (24/30,80%).The positive rate was 53%(16/30)after radiotherapy,meaning that 8 cases were converted intonegative after radiotherapy. Conclusion:The disseminated cancer cells cannot be completely eradicated by radiotherapy,suggesting further more systemic adjuvant treatment should be conducted.Early assessment of the therapeutic response might be possible by serial quantitative of CTCs.Detection of tumour cells disseminated in peripheral blood can provide clinically important data that are of value for tumour staging and possibly for prognostication.

Objective: To investigate the clinical value of immature reticulocyte count in early monitoring the suppression of bone marrow function in patients with chest tumor during postoperative chemotherapy.Methods:Forty-one patients with chest tumor(22 with lung cancer?19 with esophagus cancer) received chemotherapy after radical operation.Several indexes including reticulocyte count and white blood cell(WBC) were detected with sysmex SE-9000 automated analyzer during the procedure of postoperative chemotherapy.Results:Compared with the value before chemotherapy,HFR+MFR was significantly lower than that at 7 days after chemotherapy(P

OBJECTIVE: To implement the policy of New Medical Reform, and to promote pharmaceutical care of our hospital. METHODS: The policy of New Medical Reform was carried out and specific implementation methods for the improvement of pharmaceutical care of our hospital were analyzed. RESULTS & CONCLUSION: Prescription evaluation was applied to provide pharmaceutical care in the clinic indirectly. Clinical pharmacists went into the wards to provide direct pharmaceutical care. They supervised the utilization of antimicrobial drugs, carried out pharmacy propaganda and wrote Clinical Drug News. ADR monitoring was also carried out. The policy of New Medical Reform was implemented through above methods to promote pharmaceutical care of our hospital.

ObjectiveTo investigate the effect of psychological intervention on quality of life (QOL) of patients with mammary carcinoma.Methods64 patients with mammary carcinoma were divided into intervention group (n=36) and control group (n=28), and psychological status and QOL of patients of two groups were analyzed after treatment.ResultsThe psychological status and QOL of intervention group was better than that of control group (P<0.05～0.001).ConclusionThe psychological intervention in full stage being in hospital can improve QOL of patients with mammary carcinoma, and it is clinically significant to improve cure rate and survival rate of patients.

Objective To explore the clinical effect of probiotics combined with saline enema in the treatment of infantile diarrhea and its effect on serum inflammatory factors.Methods 186 cases of infantile diarrhea were selected,they were randomly divided into three groups according to the random number table method:probiotics group,montmorillonite group and control group.Patients in probiotics group were administered with clostridium butyricum combined with saline enema,and patients in the montmorillonite group were treated by montmorillonite combined with saline enema,and the control group was given routine treatment.Then,the clinical symptom relief time,total effective rate,serum inflammatory factor concentration and adverse reaction were observed and compared.Results The total effective rate of the probiotics group was 98.39%,and that of the montmorillonite group was 96.77%,both of which were significantly better than 80.65% of the control group,and the differences were statistically significant(χ2 =11.408,P =0.000;χ2 =8.432,P =0.018).After treatment,the serum levels of inflammatory factor tumor necrosis factor -α(TNF -α)in the probiotics group,montmorillonite group,control group were (8.30 ±2.33)pg/mL,(8.54 ± 2.47)pg/mL,(12.49 ±3.44)pg/mL,and the difference was statistically significant(F =5.120,P =0.014),also, the serum levels of interleukin -6(IL -6)in the three groups were (10.08 ±5.46)pg/mL,(11.24 ±5.84)pg/mL, (14.02 ±5.97)pg/mL,the difference was statistically significant(F =34.015,P =0.000).There were 2 cases of constipation in the montmorillonite group,the incidence rate was 3.23%,and there was no adverse reaction in the other two groups.Conclusion The effect of probiotics combined with saline enema in the treatment of infantile diarrhea is similar to that of montmorillonite,which can promote the children to alleviate the symptoms,shorten the length of hospital stay,reduce inflammation,and has no adverse reactions such as constipation,it is worth to be recom-mended widely for clinical application.

Objective To evaluate the value of lung function and blood gas analysis in early diagnosis and treatment of inter‐stitial lung disease(ILD) .Methods The pulmonary function and blood gas of 26 cases of ILD patients were tested ,and indicator change was analyzed by paired t test .Results Detection comparison showed that the indicators were significantly improved :VC in‐creased 24 .6% (P<0 .01) ,FVC increased 18 .1% (P<0 .01) ,DLCO increased 25 .6% (P<0 .01);blood gas analysis of PaCO2 decreased 21 .2% (P>0 .05) ,PaO2 increased 8 .2 % (P>0 .05) .Conclusion Lung function and blood gas measurement are impor‐tant in the early diagnosis and treatment of ILD ,which can provide the necessary basis for clinical diagnosis ,treatment and progno‐sis .

Objective Explore the species of pathogenic microorganism and analysis the antibiotic resistance in Emergency department patients in West China Hospital, finding out the evidence for clinical therapy. Methods Select the patients from May 2013 to April 2015 in Emergency department, analyses the species of pathogenic microorganism and the result of drug sensitivity test.Results In the 1 622 cases, there are 1 237 cases of gram-negative bacilli, 324 cases of gram positive coccus, 60 cases of fungus and 1cases of gram-negative coccus, accounted for 76.3%, 19.9%, 3.7%and 0.1%respectively.The top five is E.coli, acinetobacter baumannⅡ, klebsiella pneumoniae, pseudomonas aeruginosa and staphylococcus aureus, accounted for 21.4%, 16.7%, 12.4%, 11.5% and 6.5%; according to the sample species, the detection rate of sputum is highest, accounted for 46.6%, next is blood, urine, secretion; in regard to multiple resistant bacteria, detection rate of ESBL-E.coil, ESBL-KPN , CRAB and MRSA is 62.0% , 26.0%, 80.5% and 26.5%; the detection rate of VREF is 9.4% and does not find Vancomycin resistant enterococcus bacteria. Conclusions The species of pathogenic microorganism in emergency department is rich, especially gram-negative bacilli as E.coli, acinetobacter baumannⅡ, and the drug resistance situation is not optimistic. Emergency department shall communicate with microbiology laboratory timely, pay attention to the situation of the species and the drug resistance result, take rational use of antibiotic according to the results of the analysis of drug resistance.