Objective To investigate the relationship between STAT3 signal transduction pathway and proliferation as well as apotosis of vascular smooth muscle cells in rat.Methods VSMCs of rats were treated with AG490(a selective JAK2 inhibitor).MTT assay and flow cytometry were used to measure the proliferation and apoptosis.(RT-PCR) was performed to detect the levels of JAK2 mRNA expression from VSMCs.The expression of STAT3,(P-STAT3),JAK2,P-JAK2,cyclin D1 and BCL-2 proteins was measured by Western blot.Results High expression of JAK2 mRNA was detected in VSMCs and there was obvious decline of JAK2 mRNA after AG490 treating((P

Arthritis, Juvenile ; physiopathology ; Humans ; Joints ; physiopathology

OBJECTIVE:To establish the quality standard for Anti-virus chewable tablet. METHODS:TLC was used for the qualitative identification of Forsythia suspensa,Anemarrhena asphodeloides and Pogostemon cablin in Anti-virus chewable tablet, and HPLC was conducted to determine the content of forsythin in F. suspensa. The column was Diamonsil C18 with mobile phase of acetonitrile- water (20∶80,V/V) at the flow rate of 1.0 ml/min,the detection wavelength was 230 nm,column temperature was 25℃,and injection volume was 10μl. RESULTS:The test sample and the reference sample displayed same color spots on the cor-responding position in TLC diagram. The linear range of forsythin was 0.053 5-2.675 μg(r=0.999 9);RSDs of precision,stabili-ty and reproducibility tests were no more than 1.74%;average recovery was 98.94%(RSD=1.84%,n=6). CONCLUSIONS:The method is simple,rapid,accurate,reliable and reproducible,and can be used for quality control of Anti-virus chewable tablet.

Anti-Infective Agents ; administration & dosage ; therapeutic use ; Biomarkers ; blood ; Child, Preschool ; Dermatomyositis ; complications ; diagnosis ; therapy ; Edema ; etiology ; therapy ; Electromyography ; Extremities ; pathology ; physiopathology ; Hormones ; administration & dosage ; therapeutic use ; Humans ; Male ; Muscle, Skeletal ; pathology ; physiopathology ; Respiratory Tract Infections ; etiology ; therapy ; Tomography, X-Ray Computed

By integrating data of sci-tech novelty retrieaval and internet data,the paper makes a precise analysis of the potential demands,interest,interest,tendency,preferences and habits of users of novelty retrieval.From the perspective of big data technology,it analyzes the feasibility of designing targeted and deep-level services for mass user data,puts forward the framework and method of conducting deep-level and precise service recommendation based on users of sci-tech novelty retrieval,and studies the opportunity to provide these users with deep-level and precise services.

OBJECTIVE: To evaluate the efficacy of reduced glutathione in the treatment of drug-induced liver disease. METHODS: Literatures were retrieved from PubMed, Medline, EMBASE, Cochrane Library, CBM, VIP, CNKI databases by computer and from relevant Chinese medical journals by hand. Trial selection, quality evaluation and information extraction were performed according to including and excluding criteria. Meta-analysis was conducted using RevMan 5.0 software. RESULTS: The meta-analysis of 13 included RCTs showed that total effective rate, ALT and total bilirubin in trial group were significantly different from control group(P

Biomedical Research ; Communication ; Health Knowledge, Attitudes, Practice ; Medical Oncology ; Suggestion

Referred to books and literatures, the data were summarized and analyzed, and compared with western medicine phar-maceutical service to find the current problems in traditional Chinese medicine ( TCM) pharmaceutical service. According to the prob-lems and characteristics of TCM pharmaceutical service, and based on the theoretical characteristics of Chinese medicine, some sugges-tions for the development of TCM pharmaceutical service were proposed.

Objective:To reveal the status and characteristics of psychiatric nursing researches nearly five years in our country and provide some references for psychiatric nursing research development in our country in the future.Methods:The literatures related to psychiatric nursing from 2011 to 2015 were searched in China academic journal network publishing pool.Then the keywords frequency analysis and cluster analysis were conducted by using the bibliography co-occurrence analysis system (Bicomb2.0) and SPSS18.0 software and the analysis results were displayed visually.Results:In 8215 original keywords,after cleaning normatively,6643 keywords reserved finally,and there were 17 high frequency keywords among them.Through the word frequency analysis and cluster analysis,it is found that the psychiatric nursing research nearly five years in China could be classified into three major focus:psychiatric nursing safety management,psychological and rehabilitation nursing with schizophrenia patients,and promoting high quality nursing care to help improve the nursing quality.Conclusion:Psychiatric nursing research in China from 2011 to 2015 focuses on psychiatric nursing safety and quality management,and the patients with schizophrenia have been paid close attention,especially on their psychology and rehabilitation care.

Objective To investigate the effects of soluble CD40 ligand combined with LY294002,a specific inhibitor of phosphatidylinositol 3-kinase,on proliferation and invasion of gastric cancer cells (AGS ) and its potential mechanisms. Methods The effect of gradient concentration of sCD40L and its combination with LY294002 on cellular proliferation was determined using MTT assay. The ability of cellular invasion and migration was evaluated by wound healing and Transwell invasion assay. The protein expression of PI3K, p-Akt and vascular endothelia growth factor (VEGF) was tested by Western blotting. Results The inhibitory effect of cellular proliferation in sCD40L, LY294002 and sCD40L + LY294002 groups was 70. 1%, 65. 7% and 41. 3%, respectively, with significant difference (P<0. 05). And wound healing was also delayed in cells treated with sCD40L plus LY294002 when compared with those treated with sCD40L or LY294002. These results indicated that the inhibitory effect of cellular proliferation, migration and invasion was enhanced when cells were treated with sCD40L plus LY294002. The protein expression of PI3K,p-Akt and VEGF increased in cells treated with sCD40L, but reduced drastically in cells treated with sCD40L plus LY294002. Conclusion Inhibiting PI3K/Akt signaling pathway may enhance the inhibitory effect of proliferation, migration and invasion by sCD40L on AGS cells, which provide a new way for biochemical therapy of gastric cancer.