1.Study on subcretin in 8 minorities of Yunnan province.
Shan-shan WANG ; Lin LU ; Xi-rong WANG ; Lan NING ; Bin BAI ; Du-qiang JIANG ; Mei WANG ; Guang-wu AN
Chinese Journal of Epidemiology 2003;24(1):26-29
OBJECTIVETo estimate the prevalence of subcretins in 8 minorities of Yunnan province and to provide scientific basis for public health policy-making, as well as for prevention and control of iodine deficiency disorders.
METHODSFour thousand two hundred and twenty-two minority school children aged 8-12 years selected from 29 schools in 8 minority counties were measured by Combined Raven's Test in China (CRT-C(2)) and Jinyi Psychomotor test Battery (JPB).
RESULTSAverage of intelligence quotient (IQ) on 4,222 children was 91 +/- 19. Among 277 pupils with IQ between 55 - 69, 119 of them showed abnormal on JPB test and 5.4% of the children was found to have Goiter under ultrasonography. Median level of urinary iodine was 466.9 microg/L, and the qualified rate of iodized salt was 94.9%.
CONCLUSIONThe estimated prevalence rate of subcretin was 2.8%.
Child ; Child Development ; China ; epidemiology ; Congenital Hypothyroidism ; epidemiology ; Ethnic Groups ; Female ; Humans ; Intelligence Tests ; Male ; Prevalence
2.Detecting haplotypes of four Y-STR by multiplex amplification.
Qiu-ling LIU ; De-jian LU ; Ni-Mei XU
Journal of Forensic Medicine 2005;21(1):9-14
OBJECTIVE:
To establish a multiplexing Y-STR system and study haplotype frequencies of 4 Y-specific loci in China Han population.
METHODS:
DYS439, DYS390, GATA-A7.2 and DYS393 loci were amplified simultaneously and were analyzed by polyacrylamide gel electrophoresis and silver staining.
RESULTS:
When 558 unrelated male individuals from the Han population in China were tested by the multiplex system, DYS439, DYS390, GATA-A7.2 and DYS393 show 7,7,7,6 alleles, respectively. 180 different haplotypes were detected. The power of discrimination of this system was 0.9853.
CONCLUSION
The multiplex amplified system of these 4 Y-specific loci and their database are useful for human origin exploration and forensic practice.
Alleles
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China
;
Chromosomes, Human, Y
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DNA/isolation & purification*
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Electrophoresis, Polyacrylamide Gel
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Ethnicity/genetics*
;
Female
;
Forensic Medicine
;
Gene Frequency
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Haplotypes
;
Humans
;
Male
;
Polymerase Chain Reaction
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Polymorphism, Genetic
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Silver Staining
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Tandem Repeat Sequences/genetics*
6.Persistent chest pain for 4 months and progressive lower limb weakness for 2 months in a boy.
Hong-Ling WEI ; Shan LU ; Yan-Mei CHANG
Chinese Journal of Contemporary Pediatrics 2019;21(8):820-823
A boy, aged 5 years, was admitted due to chest pain for 4 months, right lower limb weakness for 2 months, and weakness of both lower limbs for 10 days. There were no symptoms of defecation/urination disorders or disturbance of consciousness, and the boy had upper motor neuron paralysis in both lower limbs, without cranial nerve involvement or sensory disorder. Spine magnetic resonance imaging revealed tumor in the spinal canal between cervical vertebra 6 and thoracic vertebra 2, which put pressure on the spinal cord. He was transferred to the department of neurosurgery for surgical treatment and fully recovered after tumor resection, and no recurrence was observed after 6 years of follow-up. The pathological diagnosis was clear cell meningioma (WHO grade II). For children with chest pain and dyskinesia, spinal meningioma should be considered.
Chest Pain
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etiology
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Child, Preschool
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Humans
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Lower Extremity
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Magnetic Resonance Imaging
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Male
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Meningeal Neoplasms
;
complications
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Meningioma
;
complications
;
Neoplasm Recurrence, Local
7.Comparison of Outcomes of Transcatheter and Surgical Procedure in Perimembranous Ventricular Septal Defect Patients with Tricuspid Regurgitation.
Xiao Ke SHANG ; Liang ZHONG ; Rong LU ; Gang Cheng ZHANG ; Mei LIU ; Qun Shan SHEN ; Xin ZHOU ; Chang Yu QIN ; Hong Mei ZHOU
Annals of the Academy of Medicine, Singapore 2016;45(7):322-325
Adolescent
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Adult
;
Cardiac Catheterization
;
economics
;
methods
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Cardiac Surgical Procedures
;
economics
;
methods
;
Cardiac Valve Annuloplasty
;
Child
;
China
;
epidemiology
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Echocardiography
;
Female
;
Heart Septal Defects, Ventricular
;
complications
;
diagnostic imaging
;
surgery
;
Humans
;
Length of Stay
;
statistics & numerical data
;
Male
;
Operative Time
;
Postoperative Complications
;
epidemiology
;
Septal Occluder Device
;
Tricuspid Valve Insufficiency
;
complications
;
diagnostic imaging
;
surgery
;
Young Adult
8.Dystrophin expression and pathology of diaphragm muscles of mdx mice after xenogenic bone marrow stem cell transplantation.
Ya-ni ZHANG ; Cheng ZHANG ; Mei-juan YU ; Shu-hui WANG ; Mei-shan LI ; Hui HUANG ; Fu XIONG ; Shan-wei FENG ; Tai-yun LIU ; Xi-lin LU
Journal of Southern Medical University 2006;26(1):53-58
OBJECTIVETo investigate the effect of bone marrow stem cell transplantation (BMT) on the diaphragm muscles of mdx mice, a mouse model of Duchenne muscular dystrophy (DMD).
METHODSThe bone marrow-derived stem cells form male SD rats was transplanted through the tail vein into 18 female 8-week-old mdx mice, which were sacrificed at 4, 8 and 12 weeks after BMT (6 at each time point), respectively. The diaphragm muscles of the mice were subjected to HE staining, immunofluorescence detection of dystrophin, reverse transcription (RT)-PCR analysis of dystrophin mRNA transcripts and PCR analysis of Sry (sex-determining region on the Y chromosome) gene, with age-matched female C57 mice and untreated mdx mice as the controls.
RESULTSThe proportion of centrally nucleated fibers (CNF) in the diaphragm muscle of the recipient mdx mice was (15.58+/-0.91) %, (12.50+/-1.87) % and (10.17+/-1.17) % at 4, 8 and 12 weeks after BMT, respectively, significantly smaller than that of untreated mdx mice [(19.5+/-1.87) %], and the fibers after BMT showed less inflammatory infiltration. Compared with the untreated mice, the recipient mdx mice showed green fluorescence on significantly more diaphragm muscle cell membranes [with the proportion of dystrophin-positive fibers of (1.00+/-0.32) %, (6.00+/-1.05) % and (11.92+/-1.11) % at 4, 8, and 12 weeks after BMT]. RT-PCR of dystrophin mRNA also demonstrated significantly higher relative levels of dystrophin in the recipient mdx mice (0.19+/-0.05, 0.26+/-0.06 and 0.36+/-0.04 at 4, 8 and 12 weeks after BMT) than in untreated mdx mice, and Sry gene was present in the recipient mice.
CONCLUSIONBMT can partially restore dystrophin expression and ameliorate the pathology in the diaphragm muscles of mdx mice, and has great potential to produce general therapeutic effect in patients with DMD.
Animals ; Bone Marrow Transplantation ; methods ; Diaphragm ; metabolism ; pathology ; Dystrophin ; biosynthesis ; genetics ; Female ; Male ; Mice ; Mice, Inbred C57BL ; Mice, Inbred mdx ; Muscular Dystrophy, Duchenne ; metabolism ; pathology ; surgery ; Rats ; Rats, Sprague-Dawley ; Transplantation, Heterologous
9.Pathogenesis of severe acute respiratory syndrome.
Ding-Mei ZHANG ; Jia-Hai LU ; Nan-Shan ZHONG
Chinese Medical Journal 2008;121(17):1722-1731
Cell Adhesion Molecules
;
physiology
;
Chemokines
;
physiology
;
Cytokines
;
physiology
;
Genetic Predisposition to Disease
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Humans
;
Lectins, C-Type
;
physiology
;
Peptidyl-Dipeptidase A
;
physiology
;
Receptors, Cell Surface
;
physiology
;
Severe Acute Respiratory Syndrome
;
etiology
;
genetics
;
immunology
10.Fetal atrial flutter in two cases with structurally normal hearts.
Shan LU ; Tong-Yan HAN ; Yan-Mei CHANG ; Yun-Pu CUI ; Xiao-Feng CHEN
Chinese Journal of Contemporary Pediatrics 2014;16(3):313-314
Atrial Flutter
;
diagnosis
;
drug therapy
;
physiopathology
;
Female
;
Humans
;
Infant, Newborn
;
Male