Anti-Bacterial Agents ; administration & dosage ; adverse effects ; therapeutic use ; Bacteria ; drug effects ; metabolism ; Bacterial Infections ; drug therapy ; Drug Resistance, Bacterial ; drug effects ; Humans ; Infant, Newborn ; Superinfection ; drug therapy

Objective To investigate the effects of Kidney nourishing decoction in the treatment of reproduce patients in serum hormone .Methods 120 cases with reproduce patients from June 2014 to June 2016 in hangzhou traditional chinese medical hospital were selected,and divided into control group and experiment group, 60 cases in each group.The control group was given artificial cycle treatment, the experiment group was treated with Kidney nourishing decoction, two groups were treated for three months.Serum sex hormone and AMH content, clinical effect and pregnancy rate were compared before and after treatment.Results Compared with before treatment, levels of FSH, LH, E2 and FSH/LH in two groups decreased, AMH content increased after treatment,the thickness of endometrium and ovarian antral follicle number increased,symptom scores decreased after treatment (P <0.05);Compared with the control group, levels of FSH, LH, E2 and FSH/LH in the experiment group were lower, AMH content were higher, the thickness of endometrium and ovarian antral follicle number in the experiment group were higher,and the symptom scores were lower (P<0.05).The total effective rate of the study group was 90.00% higher than the total effective rate of the control group 73.33% (P<0.05).There was no significant difference in adverse reaction rate between the two groups, the pregnancy rate in the experiment group 30.00% was higher than the control group 11.67% ( P <0.05 ) .Conclusion Kidney nourishing decoction can reduce the sex hormone and increase the content of AMH , and increase the pregnancy rate in the treatment of reproduce patients.

Objective To detect the changes of serum complement 3(C_3) and C_4 in neonates with hypoxic-ischemic encephalopathy(HIE) and its effects on non-specific immunological function.Methods Sixty-seven neonates with HIE and 30 normal full-term infants were recruited in the study.According to characteristic of immunological function and severity of HIE,the neonates were divided into 1-3 day group(n=32)and 4-6 day group(n=35).In each group,it was divided into mild group and moderate-severe group.The complement in serum was detected by radial immunodiffusion assay with Behring Nephelometer 100 Analyzer.Results In 1-3 day group,levels of C_3,C_4 in serum were lower than those in control group(P

0.05).In 4-6 day group:percentages of CD3+ and CD4+ cells and ratio of CD4+/CD8+ in neonates with HIE were all obviously lower than those in control group(P0.05).The level of percentages of CD3+ and CD4+ cells and ratio of CD4+/CD8+ in 9 subjects with moderate-severe HIE were remarkably higher during recovery stage than those during acute stage(P

Objective To investigate the calcium mechanism involved in the A? 25-35-induced neurotoxicity.Methods PC12 cells viability was detected by using MTT assay. Relative change of intracellular calcium concentration was measured by laser confocal microscope.Results MTT assay showed that the cell viability of the three groups (10 ?mol/L A? 25-35?10 ?mol/L A? 25-35+5 ?mol/L nifedipine?10 ?mol/L A? 25-35+10 ?mol/L nifedipine) was decreased by 34.5%, 25.1% and 11.0%,as compared with the control group. 10 ?mol/L nifedipine could protect PC12 cells from A? 25-35-induced damage. A? 25-35 of different concentration (0.1 ?mol/L, 1 ?mol/L, 10 ?mol/L, 20 ?mol/L and 30 ?mol/L) resulted in the elevation of intracellular calcium (about 6.38%, 6.42%, 62.2%, 69.3% and 107.5%) with a dose-dependent manner. Potassium (5 mmol/L, 15 mmol/L and 30 mmol/L) could increase the intracellular calcium after one minute pretreatment of different concentration A? 25-35. Both above mentioned actions were sensitive to the medium calcium and were antagonized by nifedipine, a L-voltage-gated calcium channel antagonist.Conclusion Soluble A? 25-35 may damage the neuronal calcium homeostasis in early stage and make neurons more vulnerable to physiological or pathological stimuli.

Brain Injuries ; nursing ; Custodial Care ; methods ; Humans ; Infant, Newborn ; Spectroscopy, Near-Infrared

Epilepsy, Generalized ; etiology ; Humans ; Infant, Newborn ; Male ; Malformations of Cortical Development ; complications

Arthritis, Juvenile ; classification ; diagnosis ; therapy ; Child ; Female ; Follow-Up Studies ; Humans ; Male ; Prognosis

China ; Humans ; Hypoxia-Ischemia, Brain ; diagnosis ; therapy ; Infant, Newborn