OBJECTIVE: To discuss whether the "Trusteeship of Hospital Drugstore" of mode is conducive to drug price reduction and reduction of patients' medical expense for the benefit of patients and whether this model is helpful for hospital management. METHODS: It analyzed the possible problems involved in "Trusteeship of Hospital Drugstore": such as it may lead to a "new monopolization of drug management", emergence of new "type medicine representatives", formation of "benefit league", physicians' prescribing "big prescription", thus failing to pose a threat to the "false high" price of drugs in the price chain but bringing about difficulty to hospital drug control and decreasing of drug quality etc. RESULTS & CONCLUSIONS: In view of the fact that current medical level and service income being unable to embody their actual value and the misalignment between supply and demand information of drugs,"Trusteeship of Hospital Drugstore" is still unable to free hospital from the trivial and complicated pharmaceutical affairs, nor can it surely bring down the "false high" drug price and reduce patients' medical expenses for the benefit people.

Objective To explore the effect of individualized nursing intervention on life quality and efficacy of senile patients undergoing percutaneous coronary intervention (PCI) during perioperative period. Methods 212 senile patients with PCI were divided randomly into the control group and the study group with 106 patients in each. The individualized nursing intervention and routine nursing were used in the study group, while only routine nursing was used in the control group. The patients in both groups were investigated in 3 days after PCI with questionnaire designed by the author to compare life quality and efficacy during perioperative period. Results The occurring rate of side-effect of the study group was lower than that of the control group, and the satisfaction degree, quality of life and efficacy were also higher than those of the control group. Conclusions Effective individualized nursing intervention can cor-rect the patients' pre-treatment behavior, provide more health information to the patients and give them the rules of self-nursing, so as to reduce the occurrence rate of complication, improve the patients quality of life and curative effect.

Objective To study the association of insulin resistance ( IR ) with aortic compliance impairment in patients with essential hypertension (EH). Methods Thirty-five patients with EH and 35 healthy control subjects were enrolled. The two groups were matched by sex, age and weight Blood pressure was measured and high resolution color Doppler examination was performed. Pressure-strain elastic modulus ( EP) and stiff index were calculated to reflect the change of aortic compliance in all subjects. Fasting plasma glucose (FPG) was assayed with oxidase technique, serum fasting insulin ( Fins) was assayed with chemoluminescent technique, Calculate insulin resistance index (HOMA-IR) was calculated using FPG and FINS. Results In the EH group, Fins, HOMA-IR and EP of ascending aorta were ( 10. 15 ± 1.03) mU /L, 2. 21 ± 0. 68, (250. 79 ± 85. 52 ) kN/m2, respectively,and the stiff index( β) was 15. 60 ±7. 55. In the control group, Fins,HOMA-IR, EP and stiff index β were (6. 80 ± 3. 05) mU/L, 1. 48 ± 0. 16, (183. 90 ± 64. 65 ) kN/m2 and 10. 86 ± 4. 92, respectively. There were significant difference on these above mentioned indices between the two groups (P < 0. 05 ). In the EH group, HOMA-IR were positively correlated with the ascending aorta EP and stiff index β ( r = 0.558 and 0.601 respectively, P < 0. 05 ). Conclusions Aortic compliance impairment and IR are found in patients with EH. There is significant association of IR with aortic compliance impairment Hyperinsulinemia related to insulin resistance might be an important influential factor which promote large artery remodeling in patients with EH.

Objective To verify the analytical performance of the Roche cobas 8000 automatic biochemical analyzer.Methods The intra-batch and inter-batch precisions,accuracy,linearity and reference interval were validated according to the Clinical and La-boratory Standards Institute (CLSI)documents (EP5-A2,EP15-A2,EP6-A;C28-A2 ).Results The intra-batch precision was 0.5%-2.3% and the inter-batch precision was 0.8%-3.11%,which were all less than 1/4 Tea(CLIA'88).The detection results of various items had better correlation with the target values(r2 >0.99);the linear range of various detected items were in line with the linear range provided by the specification;the quotative biological reference range conformed to the group of this laboratory services.Conclusion The various performance indexes of the Roche cobas 8000 automatic biochemical analyzer conform to the qual-ity requirements and this analyzer can be used in clinical biochemical detection work..

Dendrobii Caulis was traditionally used for promoting middle energizer, strengthening enterogastric function to light body weight, and prolonging life according to Shennong's Classic of Materia Medica and many other previous ancient books of traditional Chinese medicine. The stomach and intestine tonic effect is still approved in modern society and broadly applied in clinic, whose efficacy has been featured as replenishing yin for maintaining gastric tonicity, enhancing the production of body fluid, and clearing away heat in Chinese Pharmacopoeia 2015. This review firstly made a summary of ancient literature on strengthening enterogastric function of Dendrobii Caulis, combined with literature of modern pharmacological research and clinical application from literature search system, such as CNKI, etc. It was summarized that Dendrobii Caulis had broad bioactivities in enterogastric system including gastric mucosa protection, digest-promotion, intestinal flora modulation, intestinal immunity regulation, stimulation of digestive juice secretion, etc. Furthermore, present situation of product development was analyzed based on patent and health product search system. This review could provide a reference for further development and clinical application of Dendrobii Caulis.

OBJECTIVE: To establish the RP-HPLC specific chromatogram of Dendrobium officinale capsules for the quality control. METHODS: An Ultimate C18 column (4.6 mm × 250 mm, 5 μm) maintained at 30°C was used for all analysis. Chromatographic separation was carried out using gradient elution of methanol and 0.2% phosphoric acid aqueous solution at a flow rate of 1.0 mL · min. The detection wavelength was set at 280 nm. The similarity evaluation system for chromatographic fingerprint of TCM (National Pharmacopoeia Committee) was adopted to construct the specific chromatogram. RESULTS: The HPLC specific chromatogram of Dendrobium officinale capsules was constructed with four common specific peaks with naringenin peak as the reference. CONCLUSION: The method shows good precision and repeatability. It can be used for the quality control of Dendrobium officinale capsules and can be taken as a reference for the identification and quality control of other Dendrobium officinale-related health foods.

Objective To determine the estrogenic activity of dibutyl phthalate DBP. Methods The tested compound was dibutyl phthalate. Human estrogen-dependent MCF-7 breast cancer cells were cultured in RPMI 1640 medium containing 10% fetal bovine serum FBS. Five days before the addition of the tested compounds the cells were rinsed by phosphate balanced solution PBS and the medium was substituted with a phenol red-free RPMI 1640 medium containing 5% dextral charcoal-stripped FBS. The respective tested compound was added in fresh medium and the control cells received only the vehicle ethanol. The proliferation of MCF-7 cells was analyzed by the MTT assay growth curves mitotic index and coloning efficiency. Results Compared with the control the proliferation of tested cells treated with DBP like estradiol was markedly enhanced and the activity of the cell proliferation reached the maximum at 10-5 mol/L DBP. During log phase the mitotic index of the test cells treated with DBP and estradiol was significantly increased. The cell coloning efficiency was enhanced which was treated by 10-5 mol/L DBP only for 48 hours. The results showed the time-dependent and dose-dependent model. Conclusion Dibutyl phthalate may enhance the proliferation of human breast cancer MCF-7 cells in vitro that demonstrates an estrogenic activity of dibutyl phthalate.

Complement system is an important constituent in immune system.Membrane attack complex (C5b-9)is the final ingredient in the complement activation.Recently,a few studies showed that C5b-9 deposition was found in the diabetics with chronic complications,indicating that complement activation might be involved in the pathogenesis of diabetic complications.Our aim is to summarize some newly published works and show a prelimimary work we did in this area.We hope that more attention should be paid to the role of autoimmune mechanism in diabetic complications.

Objective To investigate the clinical efficacy of pre-hospital mild hypothermia in patients with severe craniocerebral injury,and to explore the possible mechanism by the changes of serum Malondialdehyde (MDA).Methods 120 cases of severe craniocerebral injury were divided into pre-hospital mild hypothermia group (group A,65 cases) and control group(group B,55 cases) by random number table.Patients in group A were gave pre-hospital mild hypothermia treatment,and patients in group B were gave mild hypothermia therapy only after hospitalization.Comparison of the change of serum MDA at admission,the 7th and 14th day between two groups,and the functional outcomes were evaluated by GOS score in 6 months after treatment.Results The MDA levels of two group at seventh days and 14 days were higher than that at admission [(10.4 ± 1.5)nmol/L and (8.2 ± 1.2)nmol/L in group A,(12.6 ± 1.9)nmol/L and (10.0 ± 1.4)nmol/L in group B,P ＜0.05],and it was the highest at 7th day,and its in group A was significantly lower than that in group B at 7th,14th day [(6.9 ± 1.1) nmol/L in group A,(6.6 ±1.0) nmol/L in group B,P ＜ 0.05].The good prognosis rate was 47.7％ (31 cases) in group A,higher than the 38.2％ (21 cases) in group B;The mortality rate was 12.1％ (8 cases) in group A,lower than the 21.4％ (12 cases)the group B,and the difference was statistically significant (Z =-2.132,P ＜ 0.05).Conclusion Pre-hospital mild hypothermia treatment can improve the prognosis of patients with severe craniocerebral injury and alleviate the damage of brain tissues.

Objective To investigate the relationship between HLA DRB1 gene polymorphism and susceptibility to leukopenia in patients with Graves′ disease (GD). Methods The HLA DRB1 alleles were typed by the polymerase chain reaction based sequence specific primer (PCR SSP) method in 45 GD patients with leukopenia, 50 GD patients without leukopenia and 90 normal controls. The allele frequencies in the leukopenic GD group were compared with those in the GD patients without leukopenia and control group. Results (1)Gene frequencies of HLA DRB1*08 (P