Aim The release process of an anticancer agent, DOX_HAP in rabbit liver was observed.Methods DOX and DOX_HAP were separately injectoed into rabbit livers under the guide of B type ultrasound. Dox concentrations in plasma and liver tissue of the rabbits in both groups were measured by fluorescent stectrophotometer and compared. Results The concentration of complex could existed in the liver tissue about 5 d(1 000 ng?g-1) when it was given in a dose of 2 mg?g-1. At the 10 min the concentration in plasma would be reduced about 1/2 as compared with that in the control group. Conclusions The DOX_ HAP complex not only could increase and insist in an effective concentration of DOX at the local injecting area of liver tissue, but also reduce its concerntration in the plasma. Therefore, it could decrease the toxicity and side_effect of DOX.

Objective To explore the clinical characteristics of posthepatitic cirrhosis accompanied with peptic ulcer (called as hepatogenic ulcer, HU) and the effect of quadruple therapy in this event Methods A total of 105 cases of PU were subjected in this study, and 126 patients with simple peptic ulcer (PU) served as control Endoscopy was carried out to observe the peptic ulcer, bleeding, and healing after treatment Urease testing was employed to detect Helicobacter pylori (Hp) in gastric mucosa All patients were followed up for 2 years Results Most cases of PU were confirmed to have duodenal ulcer The positive rate of Hp was 72 4% in HU group before treatment Ulcer healing rates of quadruple therapy were 65 7% and 88 5% respectively in HU group and PU group During the follow up, the rates of recurrence and rebleeding were 68 4% and 30 2% in HU group, and 48 9% and 16 6% in PU group,with very significant difference between them ( P

Objective To investigate the relationship between angiotensinogen(AGT)gene M235T polymorphism and the onset of chronic heart failure(CHF).Methods Polymerase chain reaction combined with restrictive fragment length polymorphism was used to detect the M235T variants of AGT gene in 111 CHF cases,accompanied with 110 healthy controls.Results Three kinds of AGT genotypes were detected,which were MM、MT and TT genotype separately.The frequencis of TT genotpye were higher in CHF cases than in healthy controls,and there was statistical significance in old aged group(P =0.042).The odds ratio(OR)with CHF of TT genotype was 3.367 compared with non-TT genotypes in old aged group.Conclusion The M235T polymorphism of AGT gene is associated with the occurrence of CHF in old aged group and TT genotype might be one of the genetic risk factors of CHF in such place of old aged population.

Objective:The study aimed at investigating the impact factors of FHA.Methods:The 33-pair subjects were both implemented laboratory check about ?-EP,ACTH,FSH,LH ,E2 in blood and questionnaire about Life Event Scale,EPQ and Coping Style.Results: As for the results of the single factor logistic,the impact factors were very complex.Perhaps the mechanism of FHA resulted from multi-factor interact.The main factors determining whether women suffered from FHA or not were life event,?-EP and withdrawing coping style.Conclusion:The psycholosocial factors of life events,personality and coping styles play important roles in the onset of FHA.

Objective An epitope in E1 region of hepatitis C virus (HCV) was constructed and highly expressed in a foreign epitope presenting vector based on an insect virus, flock house virus capsule protein encoding gene as a vector. Methods Using genetic engineering techniques, an epitope in E1 region of HCV was constructed in a foreign epitope presenting vector based on an insect virus, flock house virus capsule protein encoding gene as a vector and lastly constructed a chimeric gene. The chimeric gene can be put into an expressing plasmid, and expressed in either eukaryotic or prokaryotic system. The foreign epitope can be expressed in this system on the surface of the vector stereotic structure with its native conformation, and which can improve its immunogenecity. Results An epitope in E1 region of HCV was successfully cloned in three positions (aa106、aa153、aa305) of a foreign epitope presenting system based on an insect virus, flock house virus capsule protein encoding gene as a vector.The recombinant epitope in this system could be highly expressed more than 40% of total cell protein in E.coli.BL21 cell in somewhat strict conditions without IPTG inducing. Conclusions We have made this chimeric gene highly expressed in E.coli.BL21, by the way different from routine method. The results suggest that the expression method and this epitope presenting system can be used in studying immunological and biological properties of HCV and developing diagnostic reagents as well as epitope-based subunit vaccines for controlling HCV infection.

[Objective]To compare curative effects of radial head resection with internal fixation in treatment of Mason type-Ⅱ、Ⅲ fracture of radial head.[Method]Forty-five patients with Mason type-Ⅱ、Ⅲ fracture of radial head were dividered into 2 groups(A and B).The patients in group A(n=23) were performed with radial head resection.The patients in group B(n=22) were performed with open reduction and internal fixation.The results of the two groups were assessed for statistical analysis on the following aspects: pain,motion,strength and X-ray finding.Broberg and Morrey criterion were used to compared curative effect of elbow function between the two groups.[Result]The patients in the two groups were followed up for a mean time of 8 years(group A) or 3 years(group B).The difference of the pain VAS score,mean flexion/extension of the elbow,Broberg and Morrey elbow scores,carrying angle and shift of radius between the two groups were with statistical significance(P0.05).[Conclusion]In comparing with the radial head resection,open reduction and internal fixation can obtain more satisfactory elbow motion,greater muscle strength and better joint function m the treatment of Mason type-Ⅱ、Ⅲ fracture of radial head.

Child ; Humans ; Liver Diseases ; Mitochondrial Diseases

China ; epidemiology ; Databases, Bibliographic ; Humans ; Lead Poisoning ; epidemiology ; Occupational Exposure ; Workplace

OBJECTIVE:To establish a RP-HPLC method for the determination of hydroxy safflor yellow A in Orthopae_dics lotion 1.METHODS:The separation was performed on Phenomenex Luna C18 with mobile phase composed of methanol-acetonitrile-water(26∶ 2∶ 72).The flow rate was 1.0ml/min;the detective wavelength was 403nm and the column temperature was 30℃.RESULTS:At a sample size of 0.1 044? g～ 1.2 528? g(r=0.9 998),hydroxy safflor yellow A was noted to be of good linear relation with peak area score.The average recovery was 98.08%(RSD=0.52%).CONCLUSION:The method is simple,accurate,specific,sensitive,reproducible,and suitable for the quality control of Orthopaedics lotion 1.

Objective To compare the effectiveness and safety of recombinant luteinizing hormone (rLH) combined with recombinant follicle-stimulating hormone (rFSH) and rFSH alone in women undergoing in vitro fertilisation/intracytoplasmic sperm microinjection (IVF/ICSI) applied gonadotrophin-releasing hormone (GnRH) antagonist. Methods The databases including PubMed, Embase, Cochrane Library, ClinicalTrials.gov, CNKI, VIP and Wanfang Data were electronically searched to collect the randomized controlled trials (RCT) applied GnRH antagonist using rLH+rFSH or rFSH alone in IVF/ISCI cycles from inception to Dec. 2018. Following the Cochrane system evaluation and according to the criteria for inclusion and exclusion, two reviewers independently screened literature, extracted data and evaluated the bias risk for inclusion in studies, and then meta-analysis was conducted by RevMan 5.3 software. Results A total of 10 RCT studies involving 1965 patients were included, of them 988 cases in rFSH+rLH group and 977 cases in rFSH alone group. Meta-analysis showed no significant difference between rFSH alone group and rLH+rFSH group in clinical pregnancy rate (RR=1.02, 95%CI 0.82-1.27, P=0.85), ongoing pregnancy rate (RR=1.06, 95%CI 0.86-1.32, P=0.57), miscarriage rate (RR=1.38, 95%CI 0.75-2.54, P=0.29), incidence of adverse events canceled due to ovarian hyporesponsiveness (RR=0.90, 95%CI 0.42-1.93, P=0.78), and the incidence of adverse events canceled due to ovarian hyperstimulation syndrome (OHSS) (RR=1.06, 95%CI 0.56-1.99, P=0.86). Conclusions Current evidence shows that, compared with rFSH alone group, the rLH+rFSH group showed no effect on the clinical pregnancy rate, ongoing pregnancy rate, miscarriage rate, incidence of adverse events canceled due to ovarian hyporesponsiveness, and the incidence of adverse events canceled due to OHSS. The above conclusions need to be verified by more high quality research since the quality and quantity limited of included studies.