Aim The release process of an anticancer agent, DOX_HAP in rabbit liver was observed.Methods DOX and DOX_HAP were separately injectoed into rabbit livers under the guide of B type ultrasound. Dox concentrations in plasma and liver tissue of the rabbits in both groups were measured by fluorescent stectrophotometer and compared. Results The concentration of complex could existed in the liver tissue about 5 d(1 000 ng?g-1) when it was given in a dose of 2 mg?g-1. At the 10 min the concentration in plasma would be reduced about 1/2 as compared with that in the control group. Conclusions The DOX_ HAP complex not only could increase and insist in an effective concentration of DOX at the local injecting area of liver tissue, but also reduce its concerntration in the plasma. Therefore, it could decrease the toxicity and side_effect of DOX.

Objective To explore the clinical characteristics of posthepatitic cirrhosis accompanied with peptic ulcer (called as hepatogenic ulcer, HU) and the effect of quadruple therapy in this event Methods A total of 105 cases of PU were subjected in this study, and 126 patients with simple peptic ulcer (PU) served as control Endoscopy was carried out to observe the peptic ulcer, bleeding, and healing after treatment Urease testing was employed to detect Helicobacter pylori (Hp) in gastric mucosa All patients were followed up for 2 years Results Most cases of PU were confirmed to have duodenal ulcer The positive rate of Hp was 72 4% in HU group before treatment Ulcer healing rates of quadruple therapy were 65 7% and 88 5% respectively in HU group and PU group During the follow up, the rates of recurrence and rebleeding were 68 4% and 30 2% in HU group, and 48 9% and 16 6% in PU group,with very significant difference between them ( P

OBJECTIVE:To establish a method for simultaneous determination the contents determination of hydroxysafflor yel-low A and salvianolic acid B in Compound danshen oral solution. METHODS:HPLC was performed on the column of SHIMADZU ODS-C18 with mobile phase of methanol-0.5% phosphoric acid (35:65,V/V) at flow rate of 1.0 ml/min,dection wavelength was 403 nm for hydroxysafflor yellow A and 286 nm for salvianolic acid B and column temperature was 35 ℃, the volume injection was 20μl. RESULTS:The linear range was 2.01-20.10μg/ml for hydroxysafflor yellow A(r=0.999 9)and 39.80-398.00μg/ml(r=0.999 9) for salvianolic acid B. RSDs of precision,stability and reproducibility tests were lower than 2%;recoveries were 98.26%-101.25%(RSD=0.94%,n=9)and 98.70%-101.35%(RSD=0.71%,n=9),respectively. CONCLUSIONS:The method is feasible and reproducible,and can be used for the contents determination of hydroxysafflor yellow A and salvianolic acid B in Com-pound danshen oral solution.

Objective To understand the clinical characteristics of primary small vessel vasculitis with involvement of lungs in children and analyze the causes of misdiagnosis.Methods Clinical data from 16 patients with primary small vessel vasculitis were collected and their pathological,antineutrophil cytoplasmic antibody(ANCA),pulmonary lesion manifestations,image and misdiagnosis were further analyzed.Results Among 16 cases,9 cases were microscopic polyarateritis(MPA) and 4 cases were Wegeners granulomatosis(WG) and 3 cases were Churg-Strause syndrome(CSS).The ages of onset were from 3 to 14 years old with average age of 7.5 years old.Seventy-five percent(12/16) were ANCA positive.The patients with lung involvement had cough,chest pain,dyspnea,hemoptasis and asthma.Findings on lung X-ray films were variable.Some showed multiple patch shadows,others showed single or multiple masses or nodular shadows with or without cavity formation.After the treatments with corticosteroid and cyclophosphamide,10 cases turned better;including 8 cases with normal on lung X-ray films.Three cases given up;3 cases died.Conclusion It is very difficult to make diagnosis of primary small vessel vasculitis with involvement of lungs and should be paid more attentions to this issue.

Objective An epitope in E1 region of hepatitis C virus (HCV) was constructed and highly expressed in a foreign epitope presenting vector based on an insect virus, flock house virus capsule protein encoding gene as a vector. Methods Using genetic engineering techniques, an epitope in E1 region of HCV was constructed in a foreign epitope presenting vector based on an insect virus, flock house virus capsule protein encoding gene as a vector and lastly constructed a chimeric gene. The chimeric gene can be put into an expressing plasmid, and expressed in either eukaryotic or prokaryotic system. The foreign epitope can be expressed in this system on the surface of the vector stereotic structure with its native conformation, and which can improve its immunogenecity. Results An epitope in E1 region of HCV was successfully cloned in three positions (aa106、aa153、aa305) of a foreign epitope presenting system based on an insect virus, flock house virus capsule protein encoding gene as a vector.The recombinant epitope in this system could be highly expressed more than 40% of total cell protein in E.coli.BL21 cell in somewhat strict conditions without IPTG inducing. Conclusions We have made this chimeric gene highly expressed in E.coli.BL21, by the way different from routine method. The results suggest that the expression method and this epitope presenting system can be used in studying immunological and biological properties of HCV and developing diagnostic reagents as well as epitope-based subunit vaccines for controlling HCV infection.

[Objective]To compare curative effects of radial head resection with internal fixation in treatment of Mason type-Ⅱ、Ⅲ fracture of radial head.[Method]Forty-five patients with Mason type-Ⅱ、Ⅲ fracture of radial head were dividered into 2 groups(A and B).The patients in group A(n=23) were performed with radial head resection.The patients in group B(n=22) were performed with open reduction and internal fixation.The results of the two groups were assessed for statistical analysis on the following aspects: pain,motion,strength and X-ray finding.Broberg and Morrey criterion were used to compared curative effect of elbow function between the two groups.[Result]The patients in the two groups were followed up for a mean time of 8 years(group A) or 3 years(group B).The difference of the pain VAS score,mean flexion/extension of the elbow,Broberg and Morrey elbow scores,carrying angle and shift of radius between the two groups were with statistical significance(P0.05).[Conclusion]In comparing with the radial head resection,open reduction and internal fixation can obtain more satisfactory elbow motion,greater muscle strength and better joint function m the treatment of Mason type-Ⅱ、Ⅲ fracture of radial head.

Objective:The study aimed at investigating the impact factors of FHA.Methods:The 33-pair subjects were both implemented laboratory check about ?-EP,ACTH,FSH,LH ,E2 in blood and questionnaire about Life Event Scale,EPQ and Coping Style.Results: As for the results of the single factor logistic,the impact factors were very complex.Perhaps the mechanism of FHA resulted from multi-factor interact.The main factors determining whether women suffered from FHA or not were life event,?-EP and withdrawing coping style.Conclusion:The psycholosocial factors of life events,personality and coping styles play important roles in the onset of FHA.

OBJECTIVE:To establish a RP-HPLC method for the determination of hydroxy safflor yellow A in Orthopae_dics lotion 1.METHODS:The separation was performed on Phenomenex Luna C18 with mobile phase composed of methanol-acetonitrile-water(26∶ 2∶ 72).The flow rate was 1.0ml/min;the detective wavelength was 403nm and the column temperature was 30℃.RESULTS:At a sample size of 0.1 044? g～ 1.2 528? g(r=0.9 998),hydroxy safflor yellow A was noted to be of good linear relation with peak area score.The average recovery was 98.08%(RSD=0.52%).CONCLUSION:The method is simple,accurate,specific,sensitive,reproducible,and suitable for the quality control of Orthopaedics lotion 1.

China ; epidemiology ; Databases, Bibliographic ; Humans ; Lead Poisoning ; epidemiology ; Occupational Exposure ; Workplace

Objective To investigate the relationship between angiotensinogen(AGT)gene M235T polymorphism and the onset of chronic heart failure(CHF).Methods Polymerase chain reaction combined with restrictive fragment length polymorphism was used to detect the M235T variants of AGT gene in 111 CHF cases,accompanied with 110 healthy controls.Results Three kinds of AGT genotypes were detected,which were MM、MT and TT genotype separately.The frequencis of TT genotpye were higher in CHF cases than in healthy controls,and there was statistical significance in old aged group(P =0.042).The odds ratio(OR)with CHF of TT genotype was 3.367 compared with non-TT genotypes in old aged group.Conclusion The M235T polymorphism of AGT gene is associated with the occurrence of CHF in old aged group and TT genotype might be one of the genetic risk factors of CHF in such place of old aged population.