OBJECTIVE:To establish the quality standard for Anti-virus chewable tablet. METHODS:TLC was used for the qualitative identification of Forsythia suspensa,Anemarrhena asphodeloides and Pogostemon cablin in Anti-virus chewable tablet, and HPLC was conducted to determine the content of forsythin in F. suspensa. The column was Diamonsil C18 with mobile phase of acetonitrile- water (20∶80,V/V) at the flow rate of 1.0 ml/min,the detection wavelength was 230 nm,column temperature was 25℃,and injection volume was 10μl. RESULTS:The test sample and the reference sample displayed same color spots on the cor-responding position in TLC diagram. The linear range of forsythin was 0.053 5-2.675 μg(r=0.999 9);RSDs of precision,stabili-ty and reproducibility tests were no more than 1.74%;average recovery was 98.94%(RSD=1.84%,n=6). CONCLUSIONS:The method is simple,rapid,accurate,reliable and reproducible,and can be used for quality control of Anti-virus chewable tablet.

Acupuncture ; Acupuncture Therapy ; Adult ; Aged ; Aged, 80 and over ; Female ; Hiccup ; therapy ; Humans ; Male ; Middle Aged

Objective To analyze the treatment results of intracavitary hyperfractionated brachytherapy for nasopharyngeal carcinoma and to determine the optimal dose of external radiation. Methods From February 1996 to June 1998, 128 patients with T1 2 nasopharyngeal carcinoma were treated with external radiotherapy followed by intracavitary brachytherapy. The majority of them had residual tumor less than 10 mm after external radiotherapy. The external radiation doses were divided into 3 groups: 56 Gy, 60 Gy and 66 Gy. Brachytherapy was delivered with 2.5 3.0 Gy per fraction, 2 fractions a day with an interval of 6 hours. The total doses ranged from 12 to 24 Gy with a median of 18 Gy. Results The disease free survival rates at 3 and 4 years for all patients were 84.2% and 74.9%, respectively. The corresponding local relapse free survival rates were 97.1% and 92.7%, respectively. The 3 year disease free survival rates and local relapse free survival rates were 83.6% and 100% for 56 Gy group, 88.4% and 90.9% for 60 Gy group, and 84.6% and 93.3% for 66 Gy group, respectively,all with differences in significant. Radiation complications were rare. Conclusions We suggest that patients with residual tumor less than 10 mm after external radiotherapy should receive intracavitary brachytherapy as a boost. The dose of external radiotherapy for T1 2 stage NPC can be decreased to 56 Gy.

Objective To study the results of external beam radiotherapy plus 192 Ir hyperfractionated high dose rate interstitial brachytherapy (HHDR-IBT) for cancer of the oral cavity and oropharynx. Methods Fourty-eight patients with cancer of the oral cavity and oropharynx were treated by external beam radiotherapy (T 1,T 2 30～50 Gy,T 3,T 4 50～60 Gy) followed by 192 Ir HHDR-IBT delivering 15～35 Gy (30～35 Gy for T 1,T 2 and 15～30 Gy for T 3,T 4 in 250～350 cGy per fraction,two fractions per day). Over 3～5 days. Results The complete response rates at 3 months were T 1100% (5/5),T 2 85%(23/27),T 3 46%(6/13),T 4 0%(0/3) and the partial reponse rates:T 2 15% (4/27), T 3 54%(7/13), T 4 100%(3/3). The 3-year survival and disease-free survival rates were 79.4% and 55.8%,respectively.Multivariate analysis showed that TNM stage and pathalogic type were prognostic factors.Conclusions Carcinoma of the oral cavity and oropharynx treated with external beam radiotherapy combined with 192 Ir hyperfractionated high dose rate interstitial brachytherapy ia able to give a good local control for T 1,T 2 lesions with good functional preservation. For T 3,T 4 lesions,this method is able to offer a high palliation.

Objective To investigate persimmon leaf extract on secondary prevention of ischemic cerebrovascular disease, and the impact on blood pressure, blood sugar, blood lipid, hemorheology, blood rheology indexes and blood homocysteine levels in patients with ischemic cerebrovascular disease.Methods 200 cases of patients with ischemic cerebral vascular disease were chosen, which were randomly divided into observation group and control group with 100 cases in each group.Both of the groups were given conventional therapy.And the observation group received persimmon leaf extract intervention.Then the rate of recurrence of ischemic cerebrovascular disease was observed after 3 months and 6 months.At the same time, the indexes of blood pressure and blood sugar, blood lipid, hemorheology, blood flow and blood homocysteine were recorded and compared in the second morning after being selected and after 3 months and 6 months treatment.Results After treatment for 3 months, the recurrence rate of observation group was 4.40%, 3.70% of control group; after treatment for 6 months, 8.24% of observation group, 13.75% of control group, and there were no significant differences between two groups of recurrence of ischemic cerebrovascular disease.After 6 months, systolic and diastolic blood pressure in both groups were lower than before treatment.and they were lower in observation group than control group (all P<0.05).After 3, 6 months, TC, TG, LDLC levels decreased and HDLC increased compared with before treatment, and the above indexes improved more in observation group than control group (all P<0.05).After 3, 6 months, FBG, 2hPG, HbA1C levels decreased compared with before treatment, and the above indexes decreased more at the same time point in observation group than control group (all P<0.05).After 3, 6 months, HCY levels decreased compared with before treatment, and HCY decreased more at the same time point in observation group than control group ( all P<0.05 ).After 6 months, blood rheology indexes levels improved more compared with before treatment, and they improved more at the same time point in observation group than control group (all P<0.05).After 6 months, main artery blood flow velocity levels increased compared with before treatment, and they increased more at the same time point in observation group than control group ( all P<0.05 ) .Conclusion Persimmon leaf extract intervention therapy can effectively improve blood pressure , sugar, lipid, hemorheology, homocysteine and prevent platelet aggregation and thrombosis.

Objective To study the induction of IL-6 on expression and activity of tissue factor (TF)in peripheral blood monocytes(PBMCs).Methods rhIL-6 100 ng/L and rhIL-6 100 ng/L+ rhIL-6 MoAb 10?g/L were used respectively to stimulate the PBMCs for 24 h,PBMCs without any stimulation as control group,to study the changes of antigen content,mRNA expression and the ac- tivity of the TF.Results The antigen content,mRNA expression and activity of TF were increased significantly in 100 ng/L rhIL-6 group as compared with rhIL-6 100 ng/L+rhIL-6 MoAb 10?g/L and control groups(P＜0.01).Conclusions rhIL-6 can induce the increase of antigen expression,activity and mRNA expression in PBMCs,and these effects can be suppressed successfully by rhIL-6 MoAb. This study demonstrated that there was potential relations between cytokines and thrombogenesis, which may play an important role the pathogenesis of chronic rejection.

The relationship between vascular endothelial growth factor-C (VEGF-C)/vascular endothelial growth factor receptor 3 (VEGFR-3) expression and clinicopathologic features of the patients with non-small cell lung cancer (NSCLC) was investigated. The expression of VEGF-C and VEGFR-3 was assessed in 65 patients with NSCLC by immunohistochemistry. The significance of VEGF-C and VEGFR-3 expression was analyzed statistically. The results showed that VEGF-C and VEGFR-3 were highly expressed in cytoplasm and membrane in lung cancer tissues with the positive rate being 55.4 % and 52.3 % respectively, while there was no expression in the normal lung tissues. The expression of VEGF-C was significantly increased in adenocacinoma as compared to other types of NSCLC (P<0.05). The VEGFR-3 expression was closely related with lymph node metastasis (P<0.01) and TNM stage (P<0.05). There was a positive correlation between the VEGF-C and VEGFR-3 expression in NSCLC patients (r=0.658, P<0.01). It is suggested that VEGFR-3 plays an important role in the lymphatic metastasis of NSCLC. The interaction between VEGF-C and VEGFR-3 may be deeply involved in the mechanism of lung cancer metastasis.

Objective To discuss whether it is necessary to integrate metabolic indices into diagnosis of polycystic ovary syndrome (PCOS).Methods Taking ESHRE/ASRM diagnosis as the gold standard,51 women with PCOS and 47 women without PCOS were selected and divided into the intervention group and control group respectively from the Department of Gynecology in the Second Affiliated Hospital of Kunming Medical University between January 2013 and July 2014.Logistic regression based on principal component analysis and significant variables chosen through single factor analysis were used to establish the new diagnostic model which combined reproductive indices and metabolic indices.We evaluated the validity and reliability of the new diagnostic model by using ROC curve analysis.Finally,we analyzed the consistence and difference between the new diagnostic model and the gold standard.Results Thirteen significant variables were chosen using single factor analysis.ROC analysis showed that an area under the curve was 0.976 (P＜0.001) and the optimal cut-off point was 0.526 with a sensitivity of 96.08％,a specificity of 93.62％ and a consistency of93.88％.The new diagnostic model had superior validity and reliability.The two diagnostic methods had strong consistence (Kappa=0.877,P＜0.001) and no difference (x2=0.167,P=0.688).Conclusion Considering that the integration of metabolic indices does not change the diagnosis result,we come to a conclusion that it is unnecessary to integrate metabolic indices into diagnosis of PCOS.

Aim To observe the expression of migration inhibitory factor (MIF) in the enteric neurons,and to explore the nervous regulation on MIF activity in experimental colitis.Methods Colitis was induced in sensitized rat and mouse by 2,4-dinitrochlorobenzene(DNCB)enema.MIF activity was measured both in the mesentery lymphocyte(by MTT)and in the enteric neurons(by immunofluorescence double staining).6-OHDA was intraperitonealy (ip) administered to mouse before DNCB treatment.Norepinephrine(NE) was added to lymphocyte culture in vitro during MIF preparation.Results The expression of MIF protein in enteric neuron was increased in DNCB-induced colitis in rat.ip 6-OHDA in colitis mouse(38～150 mg?kg-1) resulted in a further increase of MIF activity than ip vehicle in colitis mouse (P