Bovine Lactoferricin is a fragment of polypeptide which derives from N-terminal of bovine lactoferrin when it is digested by pepsin in acid condition. It has many biological functions. This study was designed to research the antibiosis spectrum of LfcinB and the key functional active site of the LfcinB by amino acid substitution and peptide sequence modification. Antimicrobial spectrum of the artificial synthesized LfcinB was determined by agar-well diffusion method. The antibacterial active sites were confirmed by minimal inhibitory concentration assays. After the Cysteine at the third site and the tryptophan at the eighth site of LfcinB were substituted by alanine, or two cysteine of LfcinB were respectively, the minimal inhibitory concentration of the three artificially modified LfcinBs was assayed. Results showed that LfcinB had a broad-spectrum of antibiosis, it could restrain various bacterials, such as Gram-positive bacteria, Gram-negative bacteria, fungus and mycetes. LfcinB was stable to heat and pH, it could not be inactivated by many protease. The tryptophan at the eighth site and the intramolecular disulfide bond formed between two cysteins played a key role for antibiosis, as the functional active sites of LfcinB.

For natural products are one of the important sources for drug discovery, libraries and databases of natural products are significant for the development and research of natural products. At present, most of compound libraries at abroad are synthetic or combinatorial synthetic molecules, resulting to access natural products difficult; for information of natural products are scattered with different standards, it is difficult to construct convenient, comprehensive and large-scale databases for natural products. This paper reviewed the status of current accessing libraries and databases for natural products at abroad and provided some important information for the development of libraries and database for natural products.
Animals ; Biological Products ; chemistry ; pharmacology ; Databases, Factual ; Drug Design ; Humans ; Internet

Objective To explore the effect of diversified and teamwork health education mode on disease-related knowledge of coronary artery disease patients.Methods 147 cases of patients with coronary artery disease hospitalized in the general hospital of Tianjin medical university were divided into the treatment group which was given diversified health education of teamwork mode and the control group which was given traditional health education.The self-designed questionnaire was used to evaluate the effect of teamwork health education mode.Results Level of knowledge in the treatment group was significantly improved when compared with the control group,the increase amplitude of knowledge level in the treatment group was higher than the control group,and the scores of the treatment group were higher than the control group in the dimensions of diet,prevention,clinical manifestation,treatment,activity,the differences were statistically significant.Conclusions With diversified,multidisciplinary,and teamwork health education mode the patients' level of knowledge was significantly improved,the correct and positive awareness about disease were increased,and the patients were urged to take healthy behavior,so that their physical and mental state will maintain the best.

Objective To explore the influence of uncertainty in illness in compliance of respiratory function exer-cise and rehabilitation after surgery in patients with coronary artery bypass graft (CABG) surgery. Methods Mishel uncer-tainty in illness scale and compliance of respiratory function exercise scale were used to collect data in 108 patients with CABG. The correlation of the uncertainty in illness and compliance of respiratory function exercise was analyzed. Patients were divided into three groups (high score, medium score and low score groups) according to the detection of uncertainty in illness. Values of compliance of respiratory function exercise, postoperative care time, postoperative ventilation time, postop-erative hospital stay duration, 6-min walking test (6MWD) and left ventricular ejection fraction (LVEF) were compared be-tween three groups. Results The uncertainty in illness was in medium level (95.51±9.48) in patients with CABG surgery, which was negatively correlated with compliance of respiratory function exercise (r=-0.926, P<0.01). The postoperative venti-lation time and postoperative care time were less in low score group than those of medium score group and high score group. The compliance of respiratory function exercise, 6-min walking test and postoperative LVEF were better in low score group than those of medium score group and high score group. Conclusion Medical personnel should take targeted interventions to reduce the uncertainty in illness in patients, enhance the compliance of respiratory function exercise, reduce the postopera-tive complications and promote early recovery of patients.

The study is to describe the main activities of NGOs involved in programs of China AIDS Global Fund Round 5 and analyze the advantage and necessity of their involvement in HIV/AIDS control. The experience and problems of NGOs ' involvement in AIDS Global Fund Programs,as well as appropriate approaches to address them are discussed.

Objective: To explore the effect of 5-Aza-2′-deoxycytidine (5-Aza-CdR) on the molecular biological behaviors of SW480 cells and the expression of 15-hydroxyprostaglandin dehydrogenase (PGDH). Methods: Colorectal carcinoma SW480 cells were treated with 5-Aza-CdR. Cell proliferation was determined by MTT assay and plate colony formation test. The methylation status of the tumor suppressor gene PGDH was detected by methylation-specific PCR. The expression of PGDH protein was measured by Western blotting and the cell cycle was analyzed by flow cytometry. Results: It was shown that 5-Aza-CdR decreased the proliferation rate of SW480 cells compared with control group. The clony formation rate of SW480 cells decreased significantly after treatment with 5-Aza-CdR in 5 and 10μmol/L compared with the control group (35.4% and 26.5% vs 59.35%, P <0.01). The methylation degree in the promoter of PGDH gene was decreased and the expression of PGDH protein was detected after 5-Aza-CdR treatment. 5-Aza-CdR treatment induced G1 phase arrest of the SW480 cells. Conclusion: The hypermethylation of CpG islands in the promoter of PGDH gene results in the loss of PGDH protein expression in human colorectal carcinoma cell line. The demethylating agent 5-Aza-CdR could restore PGDH gene expression. These findings provide theoretic evidence for clinical treatment of human colorectal carcinoma with demethylation agent 5-Aza-CdR.

Humans ; Infant ; Lymphohistiocytosis, Hemophagocytic ; complications ; Male ; Mucocutaneous Lymph Node Syndrome ; complications

This study taking gestodene (GEST) as a model, investigated the factors affecting reservoir-type intravaginal ring (IVR)'s drug release. This paper reported a gestodene intravaginal ring of reservoir design, comprising a gestodene silicone elastomer core encased in a non-medicated silicone sheath, separately manufactured by reaction injection moulding at 80 degrees C and heating vulcanization at 130 degrees C is reported. The test investigated the factors affecting drug release through a single variable method, taking the drug release rates of 21 days as standards. When changing the thickness of the controlling sheath outside, the ratio of the first day of drug release and mean daily release (MDR), named the relatively burst effect, is closing to 1 with the thickness of controlling sheath increasing, while the 1.25 mm sheath corresponding to 1.04 controlled the burst release effectively; a positive correlation (r = 0.992 2) existed between the average drug release (Q/t) and drug loading (A) within a certain range. The C6-165 controlling sheath with high solubility of GEST is easier to achieve controlled release of the drug; GEST crystalline power is more effective to implement controlled release of drugs among difficent states of the drug. A 1/4 fractional segment core gives a relatively burst effect of 1.76, while the 1/1 and 1/2 are 1.93 and 1.87 separately, at the same drug loading, concluding that use of a fractional segment core would allow development of a suitable GEST reservoir IVR. In summary, GEST reservoir-type IVR could be adjusted by the thickness of controlling sheath, the loading of drug, the material properties of controlling sheath, the dispersion state of drug, the additive composition and structure of intravaginal ring, to control the drug release behavior and achieve the desired drug release rate.
Administration, Intravaginal ; Contraceptive Agents, Female ; administration & dosage ; Contraceptive Devices, Female ; Delayed-Action Preparations ; Drug Delivery Systems ; methods ; Norpregnenes ; administration & dosage ; chemistry ; Silicone Elastomers ; chemistry ; Solubility

Bone Marrow Transplantation ; immunology ; Case-Control Studies ; Haploidy ; Humans ; Immunity, Cellular ; immunology ; Recovery of Function ; Transplantation Conditioning ; Transplantation, Homologous