Objective To obtain stable-expressing cell lines which could constantly express tropic1808 gene and investigate the effect of tropic1808 by detecting the expression level of NF-H in PC12 cells. Methods The recombinant tropic1808 plasmid was constructed and transfected into PC12 cells.PC12 cells stable-expressing tropic1808 were established by G418 selection and confirmed by Western blot.The expression of NF-H in the PC12 stable-expressing tropic1808 was observed by RT-PCR,real time PCR,Western blot analysis and cytohistochemistry methods. Results Western blot confirmed that tropic1808 gene could express stably in PC12 cells.The data of RT-PCR,real time PCR,Western blot and cytohistochemistry all demonstrated that the expression of NF-H was increased in PC12 cells stable-expressing tropic1808.Conclusion PC12 cell lines stable-expressing tropic1808 were obtained and the expression of NF-H up-regulated in the PC12 cells stable-expressing tropic1808 gene.

Cell biology is an important course for all the students of medical school,and this course should be given with different content for the students with different academic background.The major content of cell biology course for the freshmen should be the cellular structure and the sub-cellular structure,and for the senior undergraduates,the content should include molecular basis of the cell function,and for the postgraduates,this course should focused on the molecular mechanisms of the cell function.To teach students in accordance of their background and aptitude is a good way to get a satisfied effect in a course for both teachers and students.

Anticoagulants ; therapeutic use ; Child, Preschool ; Fontan Procedure ; adverse effects ; Heparin ; therapeutic use ; Humans ; Male ; Protein-Losing Enteropathies ; drug therapy ; etiology

Objective To develop a RP-HPLC method to determine adenosine in Chongcaoqizhi granule. Method The separation was performed in a Kromasil ODS-1 column (250 mm?4.6 mm, 5 ?m) with a mobile phase of phosphate buffer solution (pH 6.5) - methanol (85∶15). The flow was 0.7 mL/min and the detective wavelength was set at 260 nm. Results The standard adenosine showed a good linear correlation at range of 1.016~12.192 ?g/mL. The linear regression equation and correlation coefficient (r) were Y =169339X -82318, r =0.9990, respectively. The average recovery of the loading was 98.02% and the RSD was 1.72%. The average content of adenosine was 12.45 ?g/g. Conclusion This method is accurate, reproducible and highly selective, and can be used for quality control of Chongcaoqizhi granule.

Objective to compare the calculated effective radiation dose variations in cardiac interventional procedures of coronary angiography(CAG)and percutaneous transluminal coronary angioplasty (PCI)by using the different tissue weighting factors recommended by ICRP Publication 60 with that recommended by ICRP Publication 103.Methods Measurements of organ doses were carried out with an anthropomorphic phantom equipped with thermoluminecent dosimetry.The effective radiation doses were separately calculated using the tissue weighting factors provided by ICRP 60 and provided by ICRP 103,and the results obtained from ICRP 60 were compared with that obtained from ICRP 103.Conversion factors of effective radiation dose to dose area product(DAP)were also calculated according to different publication. Results The variations in the effective dose were 6.88%for CAG and 8.46%for PCI.The effective dose variations in CAG and PCI were 7.25%and 8.76%for female and were 6.51%and 8.17%for male, respectively.The effective conversion factors of DAP were 0.10～0.11 and 0.13～0.14 for CAG and PCI, respectively,according to ICRP 60 and 103.Conclusion The variation of conversion factors of effective dose indicates that in making estimation of effective radiation dose by using the widely accepted conversion factors,the adjustment of organ weighting factors recommended by ICRP 103 should be considered.

Objective To identify factors influencing behavioral changes among injection drug users(IDUs)by assessing the outcome of the Kunming Sunshine Homeland Project(SHP)in 2007,so as to design more effective and relevant interventions for HIV/AIDS prevention and control in China.Method Data obtained from SHP were analyzed by Logistic regression,in which the relationship between related factors and effectiveness of the interventions were examined.Results Based on Fishbein and Guinan’s hypothesis(1996),among the three factors,i.e.attitude,perceived behavioral norm and self-efficacy,the perceived behavioral norm was the most effective one to change IDUs’ behaviors.Conclusions In designing HIV/AIDS prevention and control projects targeted to IDUs,perceived behavioral norms should be given high priority in combination with other 2 factors,and the approaches applied should match with the objective of the designed project.

Objective To assess the dose and image quality using a C-arm CT (DynaCT) system and compare the difference of image quality and radiation dose between DynaCT and Multi slice CT (MSCT). Methods Effective doses were measured from an anthropomorphic phantom with embedded thermoluminescence dosimeters (TLDs).For DynaCT,automatic exposure control (AEC) and high-quality (20 s rotation time) scans were used. For MSCT,scanning protocols for head were examined with conventional exposure conditions. Catphan phantom(Phantom Lab,USA) was used to evaluate the image quality for DynaCT and MSCT. Results For DynaCT,the effective doses from head scanning were 1.10 mSv; for MSCT,the effective doses from head scanning were 2.07 mSv,and there was a significant difference of organ dose between DynaCT and MSCT(P

Objective To investigate the influence of organ dose and effective dose on patients for different Z value during head,chest and abdomen CT scan. Methods With equispaced displacement on axis Z,organ dose and effective dose was estimated by using SR250 software with commonly used CT exposure parameters. Results Effective dose changed gradually for different Z value,some organ dose was sensitive to the Z location. Conclusion It is necessary to avoid X-ray sensitive organ in CT examination to minimize radiation risk.

Objective To strengthen the awareness that hematological tumors present with certain rheumatic symptoms for early diagnosis and treatment to a hidden neoplasm.Methods 15 patients admitted to rheumatic ward with suspected rheumatic disease were finally diagnosed as hematological tumors.Their rheumatic manifestations and occult malignancies were collected.Results A variety of rheumatic manifestations were presented.Among 15 patients,10(66.7%)had fever,6(40%)skin lesion,3(20%)arthritis,6(40%)vasculitis,3 adult onset of Still's disease,2 unelassified,and one of the each following disease:amyloidosis,dermatomyositis,polymyalgia rheumatica,and panniculitis.9 of lymphoma,3 leukemia,3 multiple myeloma were finny diagnosed. Conclusion Rheumatic disorders associated with hematological tumors include a variety of conditions.An extensive investigation for occult malignancies with some rheumatic symptoms is recommended when accompanied by specific findings of suggestive malignancy.

Objective to compare the calculated effective radiation dose variations in cardiac interventional procedures of coronary angiography (CAG) and percutaneous transluminal coronary angioplasty (PCI) by using the different tissue weighting factors recommended by ICRP Publication 60 with that recommended by ICRP Publication 103. Methods Measurements of organ doses were carried out with an anthropomorphic phantom equipped with thermoluminecent dosimetry. The effective radiation doses were separately calculated using the tissue weighting factors provided by ICRP 60 and provided by ICRP 103, and the results obtained from ICRP 60 were compared with that obtained from ICRP 103. Conversion factors of effective, radiation dose to dose area product (DAP) were also calculated according to different publication.Results The variations in the effective dose were 6.88% for CAG and 8.46% for PCI. The effective dose variations in CAG and PCI were. 7.25% and 8.76% for female and were 6.51% and 8.17% for male,respectively. The effective conversion factors of DAP were. 0.10 ～ 0.11 and 0.13 ～ 0.14 for CAG and PCI,respectively, according to ICRP 60 and 103. Conclusion The variation of conversion factors of effective dose indicates that in making estimation of effective radiation dose. by using the widely accepted conversion factors, the adjustment of organ weighting factors recommended by ICRP 103 should be considered.