Objective To establish a high sensitive spectrophotometry for determination of trace cadmium in the water. Methods A complicated ion-association complex of Cd(Ⅱ)-potassium iodide-malachite green was formed in the phosphate acid, and the addition of gelatine could enhance the sensitivity of the reaction.The maximum absorption of the ion-association complex was at 680 nm,the effect of experimental conditions such as the reagents concentration,the temperature and the influence of foreign matters were considered.Results In the optimum condition(6.0 ml of 40% potassium iodide-aseorbic acid solution,0.5 ml of 5.0 mol/L phosphate acid solution,0.5 ml of 0.5% gelatine solution,1.5 ml of 1.0?10~(-3)mol/L malachite green solution in a 25ml volumetric flask,diluted with water and mixed well and determined immediately),the linear regression equation was △A=0.011+ 0.957 c,r=0.998 5.Beer's law was obeyed in the range of 0.02 ?g/ml to 0.80 ?g/ml for Cd(Ⅱ)and the limit detection was 0.02 ?g/ ml.The composing ratio of the complex was MG:Cd:I=2:1:4,and its apparent molar absorptivity coefficient was 1.08?10~5 L/(mol? cm).The recovery rates of Cd(Ⅱ)were 97.0%-101.5%,RSDs were 1.36%-3.58%.Conclusion This method is sensitive,simple, rapid and is applicable to the determination of the trace Cd(Ⅱ)in water.

Objective: To evaluate the clinical effect of individualized enteral nutritional (EN) support in severe stroke patients.Methods: Totally 120 severe stroke patients with nutritional risk were divided into two groups according to single or double admission number.The 60 patients in the intervention group received an individualized EN with different formula and concentrations according to the clinical status,and supplemented trace elements as occasion required.In the routine group, the patients received EN with standard formula.The energy supply was 83-125 kJ· kg-1·d-1 in both groups.Blood albumin(Alb), pre-albumin(PA), hemoglobin(Hb) and vitamin C(VC) were examined before and after the 21-day nutritional support.The lung infection and gastrointestinal (GI) complications were observed, and the hospital stay and cost were recorded as well.Results: After 21 day''s nutrition support, the serum levels of Alb, PA and VC in the intervention group were not statisticly significant than before (P＞0.05), and the serum levels of Alb, PA and VC were significantly higher than those in the routine group(P＜0.05).The incidence of complications in the in the intervention group was lower than that in the routine group (P＜0.05), and the length of hospital stay and hospitalization was shorter than that in the routine group (P＜0.05).Conclusion: Individualized enteral nutritional support can significantly improve nutritional status, reduce inflammation reactions and GI complications in severe stroke patients, which is worthy of clinical use.

The incidence of severe acute pancreatitis in children is lower.But it has abrupt onset and atypical symptoms.The fatality rate is relatively high.This paper will review the progress on the diagnosis and treatment of severe acute pancreatitis.

Child ; Humans ; Inflammatory Bowel Diseases ; etiology ; pathology

Objective: To study the effect of short exposure to high levels of fluoride on cellular structure and the DSP expression of odontoblasts of mice molars. Methods: 32 4-day-old ICR mice were randomly divided into two groups. The experimental animals were received a single intraperitoneal dose of 20 mg(n =8) andlO(n=8) mg NaF/kg( body weight) respectively. Equal doses of NaCl were given to the controls (n = 8 for each group). The injected volume was kept constant (10 μ/g). After 24 hours all mice were sacrificed. HE staining and immunohistochemical staining were used to observe the structural changes and the expression of DSP in odontoblasts of mouse molar at different stages. SPSS 13.0 software package was used to analyze the results. Results: The secretory odontoblasts distorted and lost its normal column contour. The immunohistochemistry results demonstrated that the expression of DSP was more intense than that in control group. Results of statistics analysis showed there were significant differences between the two groups(P<0.01). No remarkable differences were found in mature odontoblasts. Conclusion: Short exposure to high concentration of fluoride can enhance the expression of DSP in the secretory odontoblasts,and inhibit differentiation of the odontoblasts and matrix secretion. This lead to the abnormal development of dentinogenesis.

Objective To study the clinical characteristics of the surgical treatment of cardiac valve disease in elderly patients.Methods Between January 2004 and February 2009,43 patients aged ≥65 underwent cardiac valve operation were enrolled as elderly group,85 patients aged <65 underwent the same operation served as control group in the same period,analyzed the clinical characteristics and difference between the two groups.Results The mortality of elderly group was higher than that of control group [11.6%(5/43) vs 2.4% (2/85),P<0.05].In elderly group,the duration of staying in ICU [(73.8±15.9) h],mechanical ventilation [(40.4±10.1)h],postoperative hospitalization time [(23.7±6.3) d]and the morbidity of postoperative complications [23.3%(10/43)]were higher than those of control group [(43.1±8.6)h,(28.5±5.8) h,(15.6±3.8) d,5.9%(5/85)](P<0.05 or <0.01).Conclusions Although there are high risks in patients aged 65 years and elderly with cardiac valve disease,the treatment results would be satisfactory ff select the right operating time and do full preparation with reasonable surgery strategy,the mortality and morbidity of postoperative complications could be reduced.

Objective To introduce the clinical apply of middle and small skin defect in finger injury with lateral arm free perforator flap.Methods (1) Ten cadavers were injected with a modified lead oxide-gelatin mixture.Later,CT scan,three-dimensional reconstruction of the cutaneous perforator vessels on the later arm.Then simulate the flap design.All cadavers were dissected before CT scanning.(2) Eighteen cases of middle and small skin defects in finger injury were treated with lateral arm free flap.The defect area were from 3 cm × 4 cm to 6 cm × 9 cm.Results Our research showed that the average caliber diameter of perforators of profunda brachial artery(PBA) was (0.71 ± 0.15) mm,and Posterior radial collateral artery(PRCA) was (0.94±0.22)mm.The results of pedicle length of perforator of PBA was (2.74 ±0.42) cm:(2.96 ±0.37) cm,and PRCA was (4.78 ±0.63) cm:(4.86 ±0.51) cm.3D reconstructive results showed that the perforators of PBA and PRCA dominated the lateral upper arm area.The flap of 18 cases survived after the operation.The wound of providing area was directly sutured or skin grafting and got healing.All cases were followed up for 6 months to 3 years,and the average follow-up time was 11 months.There was a little swelling on the outlooks of the flap,but the texture and sensation of the flap was good.Conclusion The lateral arm free perforator flap has a stable vessel pedicle,good texture and sensation,so it was a good method to repair middle and small skin defect in finger injury.

Objective To quantitatively detect serum Golgi protein 73 (GP73) and alpha-fetoprotein (AFP) in the patients with hepatocellular carcinoma (HCC) ,chronic hepatitis liver cirrhosis and normal healthy individuals ,to compare the detection rate of GP73 and AFP and to investigate the clinical significance of GP73 for diagnosing HCC .Methods The serum samples in normal healthy individuals and the patients with chronic hepatitis liver cirrhosis and HCC were collected and detected GP 73 and AFP by the enzyme-linked immunosorbent assay (ELISA) and the chemiluminescence method .Results The detection rates of GP73 and AFP in HCC were 75 .61% and 40 .24% respectively ,GP73 was significantly higher than AFP (P<0 .01);In chronic hepatitis liver cirrhosis ,the detection rates of GP73 and AFP were 12 .58% and 41 .67% respectively ,the detection rate of GP73 was significantly lower than that of AFP (P<0 .01);In normal healthy individuals ,the detection rates of GP73 and AFP were 1 .67% and 6 .67% re-spectively ,the difference between them had no statistical significance (P>0 .05) .The specificity of GP73 and AFP in the normal healthy individuals and the patients with chronic hepatitis kive cirrhosis were 93 .42% and 77 .78% respectively .Areas under the ROC curves of GP73 and AFP were 0 .934 1 and 0 .806 6 respectively .The HCC detection rate of the combined detection of GP73 and AFPT was 87 .80% .Conclusion The GP73 detection rate and content are extremely low in the normal healthy individuals ,the GP73 detection rate is 12 .58% in chronic hepatitis liver cirrhosis and up to 75 .6% in HCC .The ROC curve analysis and the detec-tion rate are basically consistent .GP73 has the high detection rate and strong specificity in the patients with HCC ,which is the pre-ferred marker for early diagnosis of HCC .The joint detection of GP73 and AFP can improve the early diagnosis rate of HCC .

AIM: To study if the effect of arsenic sulfide combined with IFN-α can be increased on K562 cells. METHODS: Telomerase activity was determined by PCRELISA. Flow cytometry was used to analyze the cell apoptosis. The final concentration of IFN-α and arsenic sulfide was 10 000 U/mL and 0.6 mg/L. RESULTS: The rates of apoptosis was 37.8% and 37% in K562 cells treated with IFN-α or arsenic sulfide alone for 8 days; The rates of apoptosis and inhibition of telomerase activity was 59.9% and 81.2% in K562 cells treated with IFN-α and arsenic sulfide simultaneously for 8 days, or 60.37% and 78.8% in K562 cells was treated with arsenic sulfide for 5 days after affected by IFN-α for 3 days. 71.3% telomerase activity was inhibited in K562 cells by arsenic sulfide alone for 8 days. CONCLUSION: Combination of arsenic sulfide and IFN-α can increase the apoptosis and inhibit the telomerase activity of K562 cells obviously comparing with the two drugs used alone. IFN-α maybe promote arsenic sulfide inducing apoptosis of K562 cells.

Objective To investigate the intra-aneurysmal pressure curve in the presence of endoleak after endovascular exclusion (EVE) for abdominal aortic aneurysm(AAA). Methods Infrarenal aortic aneurysms were created with bovine jugular vein segements or patches. Then they were underwent incomplete endovascular exclusion of the aneurysm and formation of endoleaks. The pressures of blood flow outside the graft into the sac were measured. Results The intrasac pressure was higher than systemic pressure in the presence of endoleak. After sealing the endoleak, pressure decreased siginificantly, and the pressure curve showed approximately linear. Conclusion The change of intra-aneurysmal pressure curve reflected the load on aneurysmal wall after EVE, and can also help to determine the endoleak existance.