Objective To evaluate the difference of internal diameter of bronchial artery in big lung cancer,small lung cancer,and normal lung with multiple slice CT.Methods MSCT angiographies of 44 patients with lung cancer confirmed by pathology were retrospectively analyzed,and 29 patients were with big lung cancer(≥3 cm)and 15 patients with small lung cancer(

Objective To investigate the effect of maternal bedside nursing care on postpartum depression of primipara.Methods Between January 2010 and December 2010,in our hospital normal full-term delivery of 400 cases were randomly divided into 2 groups with 200 cases each group.Patients in control group received traditional nursing model,namely the baby shower,massage,immunization and maternal health education were concentrated,while the experiment group implemented maternal bedside nursing care.The incidence of postpartum depression of primipara in two groups were observed.Results In the observation group,puerperal period depression incidence rate was significantly lower than that in the control group (5.5％ vs 12.5％ ;x2 =7.088,P ＜ 0.01).Conclusions The implementation of mother-to-child bedside nursing care can reduce the incidence of depression in puerperium.

Objective To investigate the bad effect of breast augmentation with PAAH injection and the technique to remove PAAH from breast effectively and safely.Methods 43 cases(86 sides)underwent operation to remove the PAAH from breast through submammary incision,followed by dressing with pressure for 3days.The patients received colored doppler ultrasonography and immunologic test before and 3 months after operation.Results Postoperative uhrasonography showed residual PAAH in breast in one case.Among the 20cases who had preoperative breast pain,the pain relieved completely in 10 cases and improved in the other 10cases.All the patients had some abnormal results in immunologic test which improved 3 months after operation.Conclusions Breast augmentation with PAAH injection can result in breast pain and other complications.It may also have bad effect on the immune system.PAAH should be removed as soon as possible.The technique through submammary incision to remove PAAH is one of the safe and reliable methods.

Objective To investigate the bad effect of breast augmentation with PAAH injection and the technique to remove PAAH from breast effectively and safely.Methods 43 cases(86 sides)underwent operation to remove the PAAH from breast through submammary incision,followed by dressing with pressure for 3days.The patients received colored doppler ultrasonography and immunologic test before and 3 months after operation.Results Postoperative uhrasonography showed residual PAAH in breast in one case.Among the 20cases who had preoperative breast pain,the pain relieved completely in 10 cases and improved in the other 10cases.All the patients had some abnormal results in immunologic test which improved 3 months after operation.Conclusions Breast augmentation with PAAH injection can result in breast pain and other complications.It may also have bad effect on the immune system.PAAH should be removed as soon as possible.The technique through submammary incision to remove PAAH is one of the safe and reliable methods.

Objective To investigate the anti-oxidative effects of alprostadil on contrast-induced nephropathy(CIN)after percuteous coronary intervention (PCI)in patients with chronic kidney disease(CKD).Methods A total of 200 patients with CKD were enrolled in our hospital.According to the random number table was divided into alprostadil 100 cases,100 cases of conventional treatment group.The levels of serum creatinine (Scr),creatinine clearance (eGFR),serum cystatin C (ScysC)and 8-hydroxy-deoxyguanine (8-OHdG)were observed before and after operation at 72 h and 7 d after operation.Results The incidence of CIN in the alprostadil group was significantly lower than that in the conventional treatment group (6% vs 12%,P<0.05).There was no significant difference in the level of Scr,eGFR,ScysC and 8-OHdG between the alprostadil group and the conventional treatment group (P>0.05).The level of Scr in the alprostadil group was significantly lower than that in the conventional treatment group at 72 h and 7 d after operation.The level of eGFR was significantly higher than that of the conventional treatment group (P<0.05).The levels of ScysC and 8-OHdG in the two groups were significantly higher than those before operation at 72 h and 7 d(P>0.05).The levels of ScysC and 8-OHdG in the alprostadil group were significantly lower than those in the conventional treatment group at 72 h and 7 d after PCI(P<0.05).Conclusion Alprostadil may improve the oxidative stress in patients with CKD and provide a preventive effect on CIN.

OBJECTIVETo identify the HBV genotype-specific tag sequence.

METHODSThe large S region sequences from 930 HBV genomes were aligned to identify the genotype-specific tag sequences. PCR was used to check the genotyping effect of these tags.

RESULTSTwo tag sequences, sequence between 149-169 and sequence between 461-483, were identified in the large S region. Using primers specific to these tag sequences, the genotype of HBV can be specifically identified.

CONCLUSIONThese tag sequences can be used for HBV genotyping.

Base Sequence ; DNA Primers ; Gene Library ; Genes, Viral ; Genotype ; Hepatitis B virus ; classification ; genetics ; Hepatitis B, Chronic ; virology ; Humans ; Molecular Sequence Data ; Polymerase Chain Reaction ; methods ; Protein Precursors ; genetics ; Sequence Analysis, DNA

With the increasingly more serious environmental pollution in China in recent years, effective intervention with PM25-induced health risks has become a major scientific issue to be addressed urgently in medical research field in China. NOD-like receptors (NLRs) are a family of cytoplasmic pattern-recognition receptors that have critical roles in innate immunity. On the basis of study progresses in international cardiovascular disease research "Fine particulate matter exposure is a modifiable risk factor for the morbidity and mortality of cardiovascular diseases", and with reference to the current understanding of pulmonary inflammation and oxidative stress in PM2.5-induced acute coronary syndrome, this study intended to investigate whether intracellular pattern recognition NL-RP3 plays a important role in the inital event of PM2.5 induced vessel inflammation as a foreign matter in the process of plaque destabilization and to thoroughly explore the underlying mechanisms responsible for PM2.5-induced acute cardiovascular events. On the other hand, it also studies the feasibility of using traditional Chinese medicine to treat plaque destabilization cause by PM2.5 exposure and discuss it's pathogenesis and intervention strategy based on TCM theory. This paper in order to provide scientific basis for social focal issues in public health proactively and offers the references for relevant research.
Air Pollutants ; toxicity ; Animals ; Environmental Exposure ; adverse effects ; Humans ; Medicine, Chinese Traditional ; methods ; Particulate Matter ; toxicity ; Plaque, Atherosclerotic ; chemically induced ; drug therapy ; mortality

OBJECTIVETo investigate the bad effect of breast augmentation with PAAH injection and the technique to remove PAAH from breast effectively and safely.

METHODS43 cases (86 sides) underwent operation to remove the PAAH from breast through submammary incision, followed by dressing with pressure for 3 days. The patients received colored doppler ultrasonography and immunologic test before and 3 months after operation.

RESULTSPostoperative ultrasonography showed residual PAAH in breast in one case. Among the 20 cases who had preoperative breast pain, the pain relieved completely in 10 cases and improved in the other 10 cases. All the patients had some abnormal results in immunologic test which improved 3 months after operation.

CONCLUSIONSBreast augmentation with PAAH injection can result in breast pain and other complications. It may also have bad effect on the immune system. PAAH should be removed as soon as possible. The technique through submammary incision to remove PAAH is one of the safe and reliable methods.

Adult ; Breast ; surgery ; Breast Implantation ; adverse effects ; Breast Implants ; adverse effects ; Device Removal ; methods ; Female ; Humans ; Mammaplasty ; adverse effects ; Young Adult

OBJECTIVETo develop a novel colorimetric method for detecting the tumor biomarker vascular endothelial growth factor (VEGF) based on aptamer and magnetic beads.

METHODSThe capture aptamer was hybridized to urease functionalized single-stranded DNA (ssDNA) and immobilize on the surface of magnetic beads by specific biotin-avidin binding. In the presence of VEGF, aptamers bound to VEGF to form a specific stem-loop structure to release the urease functionalized ssDNA. After separation, the supernatant was transferred to a tube and urea and phenol red were added. Urease hydrolyzed urea to produce ammonia to cause an increase of the pH value and a color change of phenol red. The results were inspected with either the naked eyes or by a UV spectrophotometer.

RESULTSUnder optimized conditions, the detection system showed a good linear relationship for VEGF detection in the range of 0.1 to 10 pmol/L with a detection limit as low as 0.06 pmol/L. The results of VEGF detection in the serum of patients with lung cancer were consistent with those using an ELISA Kit. The results of examination of 10 serum samples with this aptamer-based method and ELISA kit showed that the accuracy of this method was 90%.

CONCLUSIONThis aptamer-based system provides an simple and convenient method for VEGF detection with a high sensitivity and selectivity.

Aptamers, Nucleotide ; Biomarkers, Tumor ; Colorimetry ; DNA, Single-Stranded ; Humans ; Lung Neoplasms ; Nucleic Acid Hybridization ; Vascular Endothelial Growth Factor A ; analysis

Objective To investigate the correlation between morning blood pressure surge and Coronary Microvascular Dysfunction.Methods 58 cases of patients with hypertension in our hospital were given 24 h ambu-latory blood pressure monitoring(ambulatory blood pressure monitoring,ABPM).The coronary microvascular dys-function was estimated by the index of microcirculatory resistance(IMR). All cases were given biochemical test-ing,included TCH,TG,HDL-c,LDL-c and SUA.Results According to whether ABMP was arise,the patient were divided into MBPS group(n=21)and the Non-MBPS group(n=37).24 h,day,night and morning peak of systolic blood pressure were significantly higher in the morning peak group than in the average morning peak group. Multiple linear regression analysis showed that,MBPS,24 h average systolic blood pressure,day average systolic blood pressure,night average systolic blood pressure,and age were independent risk factors for coronary artery disease.Conclusion Morning blood pressure surge is closely related to severity of coronary microcirculation dysfunction. It is an independent risk factor for coronary microcirculation dysfunction. To control the blood pres-sure in patients with hypertension effectively can reduce morning peak target organ damage.