1.Biodistribution of 99 Tcm O-labeled MAG3-2-nitroimidazole hypoxia imaging agents with different spacers in S180 scarcoma-bearing mice
Lei MEI ; Zhi YANG ; Taiwei CHU
Chinese Journal of Nuclear Medicine and Molecular Imaging 2017;37(1):25-30
Objective To evaluate the hypoxia selectivity of 99 Tcm O?labeld MAG3?nitroimidazole complexes with different spacers. Methods Four kinds of 99 Tcm O?labeled MAG3?2?nitroimidazole hypoxia imaging agents with different spacers were synthesized and radiolabeled. The stability and lipid solubility of BzMAG3?2NIEA(1), BzMAG3?2NIPA(2), BzMAG3?2NIHA(3) and BzMAG3?2NIUA(4) were measured. The uptake was investigated by biodistribution experiment using S180?bearing mice. Two?sample t test was used for statistical analysis. Results All 4 99 Tcm O?MAG3 complexes were stable and negatively charged, showing an increasing trend in fat solubility with the increase of spacer length. In biodistribution study, tumor uptake of 99TcmO?1 and 99TcmO?2 with medium? and short?carbon chain were (0.67±0.18) and (0?65±0.18) %ID/g 2 h post injection, which were (0.19±0.03) and (0.39±0.05) %ID/g for 99TcmO?3 and 99TcmO?4 with long?carbon chain (t=2.78-5.88, all P<0.05). Conclusion Molecular structure of spacers has a significant effect on the physicochemical properties and tumor targeting of 99 Tcm O?labeled MAG3?2?nitroimidazole hypoxia imaging agents, such that the medium?and short?carbon chain spacers show the best hypoxia?selective property.
2.Advances in lymphangiogenesis and metastasis of tumor.
Xiao-chu YAN ; Dong-mei YU ; Feng-xuan LIU
Chinese Journal of Pathology 2005;34(6):370-372
Animals
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Humans
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Lymph Nodes
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pathology
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Lymphangiogenesis
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physiology
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Lymphatic Metastasis
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Lymphatic Vessels
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metabolism
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pathology
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Neoplasms
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metabolism
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pathology
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Vascular Endothelial Growth Factor C
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metabolism
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physiology
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Vascular Endothelial Growth Factor D
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metabolism
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physiology
3.Effect of manual massage on relapse rate and quality of life of grand mal epilepsy patients
Dongjin SUN ; Renxiang SHANG ; Hua YU ; Ruiqing YANG ; Mei CHU
Chinese Journal of Practical Nursing 2013;29(36):12-14
Objective The aim of the study is to study the effect of manual massage on relapse rate and quality of life of grand mal epilepsy patients.Methods Using prospective research method,clinical data of patients with epileptic seizures and without attack up to 3 years and undergoing withdrawal of drugs for one year were collected.The grand mal epilepsy patients were randomly divided into the experimental group and the control group (76 cases in each group).The experimental group was given manual massage.The control group was not given manual massage.Both groups were given follow-up for 1 year.The relapse rate and quality of life were compared between two groups.Results The relapse rate of the ex perimental group was 17%,and that of the control group was 33%,the difference was significant.The score of life quality in the experimental group was significantly better than that of the control group.Conclusions Manual massage can reduce relapse rate and improve quality of life for patients with epileptic seizures after withdrawal of drugs,which is worthy of wide application.
4.A study on efficacy of Carbamazepine with manual massage on grand mal epilepsy
Mei CHU ; Xiaoyan CHEN ; Hongyan YU ; Hong ZHANG ; Shujuan YU
Chinese Journal of Practical Nursing 2013;29(33):9-10
Objective The aim of the study is to explore the efficacy of Carbamazepine with manual massage on grand mal epilepsy.Methods Using a prospective research method,the grand mal epilepsy patients were randomly divided into the experimental group(40 cases)and the control group(40 cases).The experimental group was given manual massage based on oral Carbamazepine treatment.The control group was given oral Carbamazepine treatment.The treatment effect was compared between two groups.Results The curative efficacy of the experimental group was 92.5%,higher than 80.0% of the control group.Conclusions Carbamazepine with manual massage for treatment of grand mal epilepsy can obviously improve curative efficacy,which is worthy of popularization and application.
5.Expression of BAFF and its specific receptor BAFF-R in patients with B-cell non-Hodgkin′s lympho-ma and their significance
Jiang PU ; Shaopeng CHU ; Mei WANG ; Chen QIAN ; Xianjuan SHEN
Chinese Journal of Microbiology and Immunology 2015;(11):821-826
Objective To investigate the expression of B-cell activating factor ( BAFF ) and its specific receptor BAFF-R in patients with B-cell non-Hodgkin′s lymphoma ( B-NHL) and to analyze the cor-relations between BAFF and the development of B-NHL.Methods RTQ-PCR and Western blot assay were used to measure the expression of BAFF and its specific receptor BAFF-R in patients with B-NHL.Fluores-cence immunocytochemical staining was used to determine the localization of BAFF and BAFF-R in Raji cells, a B-NHL cell line.The expression of BAFF in tumor tissues from patients with B-NHL of different his-tologic subtypes was measured by immunohistochemistry.WST proliferation and TUNEL assays were used to evaluate the effects of BAFF and BAFF-R on the proliferation, survival rate and apoptosis of Raji cells.Lin-ear correlations between the concentrations of lactate dehydrogenase ( LDH) and the expression of BAFF and BAFF at mRNA and protein levels in patients with B-NHL were analyzed.Results BAFF and its specific receptor BAFF-R were expressed in Raji cells and played an important role in the survival and proliferation of B-NHL cell line.The expression of BAFF in tumor cells from patients with B-NHL varied with the different histologic subtypes of B-NHL.Patients with small B-cell malignant lymphoma, large B-cell lymphoma ( LBCL) , mucosa-associated lymphoid tissue lymphoma ( MALT lymphoma) and follicular lymphoma showed higher levels of BAFF, while those with mantle cell lymphoma showed lower levels of BAFF.Compared with the healthy subjects, patients with B-NHL showed significantly increased expression of BAFF at mRNA and protein levels.The levels of LDH were closely related to the expression of BAFF at mRNA and protein lev-els.Conclusion BAFF and its specific receptor BAFF-R might play an important role in the growth and survival of malignant B cells.
6.Treatment of bochdalek′s hernia and defect of diaphragm in children
chao-mei, XIANG ; chen-chu, ZHU ; dai-xian, GONG
Journal of Applied Clinical Pediatrics 1992;0(05):-
Objective To study the diagnosis and treatment of bochdalek′s hernia in children, especially to introduce the methods of repairing the defect of diaphragm using rib-flap and terylene fabric.Methods The diagnosis, misdiagnosis and methods of repairing of diaphragm in 10 cases of bochdalek′s hernia were retrospectively reviewed, and the findings of reconstruction of diaphragm in 2 cases of defect of diaphragm were analyzed.Results All of the 12 cases were misdiagnosed for the first time. Ten cases were cured through abdominal surgery and 2 cases were cured through reconstruction of diaphragm.Conclusions Bochdalek′s hernias in children are lack of specific clinical manifestation and often misdiagnosed before operation. Early surgical treatment is necessary once the diagnosis is established, and the defect of diaphragm should be reconstructed. The repairment of diaphragm using rib-flap and terylene fabric is a practical methods.
7.New modified iris suture technique for pupillary dilation in aphakic eyes during vitreoretinal surgery
Feng-Mei, HAN ; Quan-Hong, HAN ; Yan-Hua, CHU
International Eye Science 2011;11(1):4-5
To describe a modified simple iris suture for pupillary dilation technique during vitrectomy in cases with a miotic pupil.Four translimbal incisions were created with a sharp straight blade at 1:30,10:30,4:30,and 7:30 o'clock,respectively.The straight needle of 10-0 polypropylene suture and a Sinskey IOL hook was used to displace the pupillary margin toward the limbus.In 3 cases,four sutures caused a 6-mm to 9-mm square-shaped pupil,and the pupil was allowed to return to a smaller size at the end of the operation.It is simple and may reduce postoperative complications.
8.REGULATION OF INTESTINAL DYSBACTERIOSIS OF BURNED RATS BY MICROECOSYSTEM REGULATORS
Long MEI ; Yonglie CHU ; Dexin XIONG ; Xiaofeng ZHU
Journal of Pharmaceutical Analysis 2001;13(1):39-41
Objective To investigate the feasibility of using synbiotics and probiotics to prevent and cure intestinal dysbacteriosis after burn. Methods Burned rats were fed with synbiotics and probiotics reagents, and the amounts of major intestinal florae in caecal contents were detected. Results The major physiological anaerobes were mostly stable, and the conditioned pathogens had no abnormal. Conclusion The micro-ecosystem regulator can quickly supplement the decreased physiological anaerobes caused by burning,and avoid the occurrence of dysbacteriosis.
9.Effects of postpartum lactation of puerperal women with qi and blood deficiency treated by catgut embedding therapy.
Li-Ping LI ; Ye-Hua BAO ; Jia-Mei CHU
Chinese Acupuncture & Moxibustion 2014;34(2):189-190
Acupuncture Therapy
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Adult
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Catgut
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utilization
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Female
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Humans
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Lactation
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Lactation Disorders
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physiopathology
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therapy
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Postpartum Period
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physiology
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Qi
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Young Adult
10.Changes of Blood Lipid,Blood Rheology and Microcirculation of Model Rats of Diabetes Ⅱ with Heart Blood Stasis Syndrome
Meng-Zhou XIE ; Hao-Mei TIAN ; Chu-Tao CHEN ;
Journal of Traditional Chinese Medicine 1992;0(12):-
Objective To explore the relations among blood lipid,blood rheology and microcirculation of bulbar conjunctiva of model rats of diabetesⅡwith heart blood stasis syndrome.Methods The rats were fed with high fat and caloric feed.Intraperitoneal injection of streptozotocin(STZ)in small dosage was applied to make the model of diabetesⅡ.Then the model of heart blood stasis syndrome was made through introperitoneal injecting dexamethasone and adrenalin.Eight hours after empty stomach,the rats were anaesthetized to measure the microcireulation of bulbar conjunctiva and test the blood lipid and carotid blood rheology.Results The indices of blood lipid,blood rheology and bulbar eonjunetiva microcirculation of model rats were all changed to abnormal,tallying with the changes of cardiovascular complications of diabetes.