Cell biology is an important course for all the students of medical school,and this course should be given with different content for the students with different academic background.The major content of cell biology course for the freshmen should be the cellular structure and the sub-cellular structure,and for the senior undergraduates,the content should include molecular basis of the cell function,and for the postgraduates,this course should focused on the molecular mechanisms of the cell function.To teach students in accordance of their background and aptitude is a good way to get a satisfied effect in a course for both teachers and students.

Objective To obtain stable-expressing cell lines which could constantly express tropic1808 gene and investigate the effect of tropic1808 by detecting the expression level of NF-H in PC12 cells. Methods The recombinant tropic1808 plasmid was constructed and transfected into PC12 cells.PC12 cells stable-expressing tropic1808 were established by G418 selection and confirmed by Western blot.The expression of NF-H in the PC12 stable-expressing tropic1808 was observed by RT-PCR,real time PCR,Western blot analysis and cytohistochemistry methods. Results Western blot confirmed that tropic1808 gene could express stably in PC12 cells.The data of RT-PCR,real time PCR,Western blot and cytohistochemistry all demonstrated that the expression of NF-H was increased in PC12 cells stable-expressing tropic1808.Conclusion PC12 cell lines stable-expressing tropic1808 were obtained and the expression of NF-H up-regulated in the PC12 cells stable-expressing tropic1808 gene.

Anticoagulants ; therapeutic use ; Child, Preschool ; Fontan Procedure ; adverse effects ; Heparin ; therapeutic use ; Humans ; Male ; Protein-Losing Enteropathies ; drug therapy ; etiology

Cushing's syndrome is caused by the excessive secretion of cortisol from the adrenal cortex.Hypertension is one of the most distinguishing features of Cushing's syndrome,as it is present in about 80% of adult patients.The various pathogenetic mechanisms that have been proposed for the development of cortisol-induced hypertension in Cushing's syndrome and its management are discussed.

Aim To observe the effect of metformin(MF)on inhibinB,activinA and follistatin in rat of polycystic ovary(PCO),analyzing the possible therapeutic mechanism and therapeutical effect from ovaries local cytokine.Methods The PCO rat models were set by insulin combined with human chorionic gonadotrophin(HCG).The levels of serum gonadal hormone and insulin(INS)were detected by radioimmunoassay.Using the sandwich ELISA technique the expressed level of serum inhibinB(INHB),activinA(ACTA)and follistatin(FS)was detected.Results Metformin could decrease the level of testosterone(T),luteotropic hormone(LH)and INS in rat serum,but it increased the level of ACTA in rat serum and the rate of ACTA/INHB.There was negative correlation between INS and ACTA,and positive correlation between INS and INHB.Conclusion MF can regulate the expression of ovaries local cytokines such as INHB,ACTA and FS.

Objective to compare the calculated effective radiation dose variations in cardiac interventional procedures of coronary angiography(CAG)and percutaneous transluminal coronary angioplasty (PCI)by using the different tissue weighting factors recommended by ICRP Publication 60 with that recommended by ICRP Publication 103.Methods Measurements of organ doses were carried out with an anthropomorphic phantom equipped with thermoluminecent dosimetry.The effective radiation doses were separately calculated using the tissue weighting factors provided by ICRP 60 and provided by ICRP 103,and the results obtained from ICRP 60 were compared with that obtained from ICRP 103.Conversion factors of effective radiation dose to dose area product(DAP)were also calculated according to different publication. Results The variations in the effective dose were 6.88%for CAG and 8.46%for PCI.The effective dose variations in CAG and PCI were 7.25%and 8.76%for female and were 6.51%and 8.17%for male, respectively.The effective conversion factors of DAP were 0.10～0.11 and 0.13～0.14 for CAG and PCI, respectively,according to ICRP 60 and 103.Conclusion The variation of conversion factors of effective dose indicates that in making estimation of effective radiation dose by using the widely accepted conversion factors,the adjustment of organ weighting factors recommended by ICRP 103 should be considered.

Objective To identify factors influencing behavioral changes among injection drug users(IDUs)by assessing the outcome of the Kunming Sunshine Homeland Project(SHP)in 2007,so as to design more effective and relevant interventions for HIV/AIDS prevention and control in China.Method Data obtained from SHP were analyzed by Logistic regression,in which the relationship between related factors and effectiveness of the interventions were examined.Results Based on Fishbein and Guinan’s hypothesis(1996),among the three factors,i.e.attitude,perceived behavioral norm and self-efficacy,the perceived behavioral norm was the most effective one to change IDUs’ behaviors.Conclusions In designing HIV/AIDS prevention and control projects targeted to IDUs,perceived behavioral norms should be given high priority in combination with other 2 factors,and the approaches applied should match with the objective of the designed project.

Objective To evaluate the effect of a new schistosomiasis control strategy based on the infection source control in four-lake regions of Hubei Province. Methods The new strategy based on the infection source control included the safe water sup-ply,feces harmless treatment,replacing cattle with machine,captive livestock,chemotherapy,Oncomelania hupensis snail con-trol in susceptible areas,hardening ditches,etc,and the new control strategy was implemented for 7 years in 9 counties(cities or districts)of the four-lake regions. The schistosomiasis situations were investigated before and after the intervention of the new strat-egy. Results The coverage rates of feces harmless toilets(three format toilets),biogas digesters,and safe water supply were 42.00%,23.16% and 93.76%,respectively. The number of ditch hardening was 1 960;the area of eliminating snails was 1 378.42 hm2;the number of persons who received the health education was 3 524 818 accounting for 92.17%of the total popula-tion;the number of person-time of taking active protection measures was 516 636 963. The average annual decline rates of schisto-some infection were 24.42%and 38.38%in residents and cattle,respectively. The endemic villages decreased by 4.77%,the vil-lages with snails decreased by 7.05%,and the villages with snails accounted between 63.19%and 66.21%of the total endemic vil-lages. The area with snails decreased by 90.35%,and the advance schistosomiasis patients decreased by 5.55%,and the fatality rate of advanced schistosomiasis patients decreased by 1.24%. No acute schistosomiasis patients occurred. Type One and Two en-demic(heavy endemic)villages decreased to zero;Type Three endemic(moderate endemic)villages decreased by 10.22%;but Type Four endemic(mild endemic)villages increased by 66.38%;and Type Five endemic(no endemic for 5 years)villages de-creased by 0.22%. The standards of schistosomiasis transmission controlled have achieved on schedule. Conclusion The imple-mentation of the new schistosomiasis control strategy based on the infection source control in four-lake regions of Hubei Province can effectively control schistosomiasis.

Myocardial infarction (MI) is a major cause of death and disability worldwide.The definition of myocardial infarction has been updated.Released in 2012,Third Universal Definition of Myocardial Infarction of ESC/ACCF/AHA/WHF Expert Consensus,made the clinical application value of troponin clear and suggested to monitor the dynamic changes of cTn in patients with suspected,emphasized the importance of clinical differential diagnosis.In consensus,it also noted that the application of high sensitive troponin can lead to false positives results,and pointeds out that in the assessment of cardiac injury associated with percutaneous or surgical coronary procedures,the rational cut-off value of cTn remains controversival.In addition to acute coronary syndrome,cTn elevations are common in many disease states,and with the improvement of detection sensitivity,low level increase of cTn,will be more.In acute and chronic heart failure,the cTn is elevated,and ACS may be the cause of worsening heart failure,So we should identify whether the cTn elevation is pure from the myocardial injury of heart failure or myocardial necrosis caused by ACS.Nowadays,the increased incidence of myocardial infarction has much to do with the wide application of cTn.Clinical applications of cardiac biomarkers and imaging techniques are flexible as the differences in the diagnostic techniques of myocardial infarction among countries and regions result.

20 patients with myofascial pain dysfunction syndrome(MPDS)were were treated by NTI-tss splint only during night for 2 months.After treatment,VAS decreased(P <0.01)and active maximum mouth opening degree increased(P <0.01).NTI-tss splint is ef-fective in the treatment of MPDS.Its side effect needs to be further observed.