Cell biology is an important course for all the students of medical school,and this course should be given with different content for the students with different academic background.The major content of cell biology course for the freshmen should be the cellular structure and the sub-cellular structure,and for the senior undergraduates,the content should include molecular basis of the cell function,and for the postgraduates,this course should focused on the molecular mechanisms of the cell function.To teach students in accordance of their background and aptitude is a good way to get a satisfied effect in a course for both teachers and students.

Objective To obtain stable-expressing cell lines which could constantly express tropic1808 gene and investigate the effect of tropic1808 by detecting the expression level of NF-H in PC12 cells. Methods The recombinant tropic1808 plasmid was constructed and transfected into PC12 cells.PC12 cells stable-expressing tropic1808 were established by G418 selection and confirmed by Western blot.The expression of NF-H in the PC12 stable-expressing tropic1808 was observed by RT-PCR,real time PCR,Western blot analysis and cytohistochemistry methods. Results Western blot confirmed that tropic1808 gene could express stably in PC12 cells.The data of RT-PCR,real time PCR,Western blot and cytohistochemistry all demonstrated that the expression of NF-H was increased in PC12 cells stable-expressing tropic1808.Conclusion PC12 cell lines stable-expressing tropic1808 were obtained and the expression of NF-H up-regulated in the PC12 cells stable-expressing tropic1808 gene.

Anticoagulants ; therapeutic use ; Child, Preschool ; Fontan Procedure ; adverse effects ; Heparin ; therapeutic use ; Humans ; Male ; Protein-Losing Enteropathies ; drug therapy ; etiology

Bovine Lactoferricin is a fragment of polypeptide which derives from N-terminal of bovine lactoferrin when it is digested by pepsin in acid condition. It has many biological functions. This study was designed to research the antibiosis spectrum of LfcinB and the key functional active site of the LfcinB by amino acid substitution and peptide sequence modification. Antimicrobial spectrum of the artificial synthesized LfcinB was determined by agar-well diffusion method. The antibacterial active sites were confirmed by minimal inhibitory concentration assays. After the Cysteine at the third site and the tryptophan at the eighth site of LfcinB were substituted by alanine, or two cysteine of LfcinB were respectively, the minimal inhibitory concentration of the three artificially modified LfcinBs was assayed. Results showed that LfcinB had a broad-spectrum of antibiosis, it could restrain various bacterials, such as Gram-positive bacteria, Gram-negative bacteria, fungus and mycetes. LfcinB was stable to heat and pH, it could not be inactivated by many protease. The tryptophan at the eighth site and the intramolecular disulfide bond formed between two cysteins played a key role for antibiosis, as the functional active sites of LfcinB.

Objective To establish a high sensitive spectrophotometry for determination of trace cadmium in the water. Methods A complicated ion-association complex of Cd(Ⅱ)-potassium iodide-malachite green was formed in the phosphate acid, and the addition of gelatine could enhance the sensitivity of the reaction.The maximum absorption of the ion-association complex was at 680 nm,the effect of experimental conditions such as the reagents concentration,the temperature and the influence of foreign matters were considered.Results In the optimum condition(6.0 ml of 40% potassium iodide-aseorbic acid solution,0.5 ml of 5.0 mol/L phosphate acid solution,0.5 ml of 0.5% gelatine solution,1.5 ml of 1.0?10~(-3)mol/L malachite green solution in a 25ml volumetric flask,diluted with water and mixed well and determined immediately),the linear regression equation was △A=0.011+ 0.957 c,r=0.998 5.Beer's law was obeyed in the range of 0.02 ?g/ml to 0.80 ?g/ml for Cd(Ⅱ)and the limit detection was 0.02 ?g/ ml.The composing ratio of the complex was MG:Cd:I=2:1:4,and its apparent molar absorptivity coefficient was 1.08?10~5 L/(mol? cm).The recovery rates of Cd(Ⅱ)were 97.0%-101.5%,RSDs were 1.36%-3.58%.Conclusion This method is sensitive,simple, rapid and is applicable to the determination of the trace Cd(Ⅱ)in water.

Objective: To investigate the effects of Zhu Yun Pill(ZYP) in the treatment of infertility due to luteal phase defect(LPD).Methods: 163 infertility patients due to LPD were randomly divided into three groups in terms of ZYP group,progesterone group and combination group(ZYP and progesterone).Then the therapeutic efficacy was judged by the pregnancy rate(PR),endometrial biopsy,the level of serum hormone and basal body temperature before and after the treatment.Result: 40 women were pregnant in ZYP group(cure rate,33.33%),8 in the progesterone group(cure rate,26.67%) and 9 in the combination group(cure rate, 69.23%).The symptomatic amelioration and total effective rate of ZYP group were obviously higher than single exogenous progesterone group(P

Objective To explore the relationship between interleukin-8 (IL-8) gene 1633C/T polymorphism and late-onset Alzheimer's disease in order to provide genetic reference data for late-onset Alzheimer's disease.Methods Polymorphism distribution of IL-8 gene 1633C/T in 80 LOAD and 80 normal controls were detected by polymerase chain reaction-restriction fragment length polymorphism(PCRRFLP) analysis.The alleles and gene frequencies type were calculated by the direct counting.Type of gene was proceeded with the Hardy-Weinberg balance estimate.The other statistical analyses were carried out with SPSS13.0 software.The genotype frequencies and allele frequencies were compared with the Chisquare test (x2 test).Results For IL-8 gene1633 C/T,the genotype frequencies (CC,20.0％ ; CT,41.2％ ; TT,38.8％) in the LOAD group were not significantly different from those (CC,11.3％ ; CT,40.0％ ; TT,48.7％) in the control group (P ＞ 0.05).Conclusions IL-8 gene 1633 C/T polymorphism is probably not related to the genetic susceptibility of late-onset Alzheimer's disease.

Proteome is defined as all proteins expressed in a cell, a tissue or an organism. Proteomics, a science of understanding the life activity law at the whole protein level, takes proteome as a research object; it is also a scientific method for quantitative detection of one or more protein populations or proteomes in a cell or tissue. Cerebral ischemic preconditioning has been one of the research hotspots in the field of neuroscience for decades. Its neuroprotec-five mechanism is associated with the changes of protein expression levels after cerebral ischemia, Therefore, proteomics has become more and more important in its mechanism study. This article mainly presents the major techniques of proteomics and their application in the studies of cerebral ischemic preconditioning.

Objective To assess the dose and image quality using a C-arm CT (DynaCT) system and compare the difference of image quality and radiation dose between DynaCT and Multi slice CT (MSCT). Methods Effective doses were measured from an anthropomorphic phantom with embedded thermoluminescence dosimeters (TLDs).For DynaCT,automatic exposure control (AEC) and high-quality (20 s rotation time) scans were used. For MSCT,scanning protocols for head were examined with conventional exposure conditions. Catphan phantom(Phantom Lab,USA) was used to evaluate the image quality for DynaCT and MSCT. Results For DynaCT,the effective doses from head scanning were 1.10 mSv; for MSCT,the effective doses from head scanning were 2.07 mSv,and there was a significant difference of organ dose between DynaCT and MSCT(P

Objective To investigate the influence of organ dose and effective dose on patients for different Z value during head,chest and abdomen CT scan. Methods With equispaced displacement on axis Z,organ dose and effective dose was estimated by using SR250 software with commonly used CT exposure parameters. Results Effective dose changed gradually for different Z value,some organ dose was sensitive to the Z location. Conclusion It is necessary to avoid X-ray sensitive organ in CT examination to minimize radiation risk.