Programmed cell death(PCD)of human leukemic HL-60 cell and human poorly differentiated gastric adenocarcinoma cell BGS-180 induced by efoposid(VP-16) was preliminarily observed comparatively in the same experimental condition through rate of cell death, DNA agarose gels electrophoresis and terminal deoxynucleotidyl transferase(TdT) mediated biotin-11-dUTP nickend labeling(TUNEL).It was found that apoptosis is the main pattern of HL-60 cell death induced by low does VP-16 in a short time, and it can be suppressed when protein kinase C(PKC) is activated. The main pattern of BGS-180 cell death induced by VP-16 is necrosis, and PCK activation does not affect its necrosis rate. Extracellular Ca2+ reduction do not affect BGS-180 and HL-60 cell death rate. The mechanism of VP-16 action on BGS-180 and HL-60 cell is different, apoptosis is not the main pattern of cell BGS-180 death induced by VP-16.

To evaluate the incidence of sub-clinical neck metastasis and its surgical treatment in patients with oral squamous cell carcinoma (OSCC) without clear evidences for lymph nodes metastasis, a total of 205 patients with OSCC in N0 and N1 stage(UICC 1987′s classification) were included in the study.Their clinical and histopathological files were retrospectively studied and patients were followed up after operation. Histopathological positive lymph nodes were found in 10.8%(13/120) of patients,and postoperative lymph nodes metastasis occurred in 16.5%(14/85) of patients without neck dissections.There were no significant differences between N0 and N1 groups.Neck recurrence occurred in 11.9%(5/42) of patients after functional neck dissections.The rate was higher in patients with bucal lesions.Patients undergoing radical neck dissections also had neck recurrence with an incidence of 6.4%.But most of them occurred on contralateral side. It suggested that subclinical metastasis of OSCC with clinically negative neck lymph nodes could not be neglected.Functional neck dissection should include the lymph nodes of level Ⅲ,especially for cancers located on bucca mucosa and tongue.Bilateral neck dissection is necessary for large primary lesions spreading to the contralateral side.

Tea polyphenols were tried to induce apoptosis of human cultured hepatocellular carcinoma cell line HepG-Ⅱ. MTT assay, DNA agarose gel electrophoresis, transmission electron microscopy , fluorescence decoration and DNA end labeling method (Tunel) were used to identify apoptosis. Having been treated by tea polyphenols in 250μg/ml , HepG-Ⅱcell apoptosis was induced. The induction of apoptosis was the dose dependent. Chromatin condensation, apoptotic body formation, fluorescence of yellow green and pellet were observed. Agarose gel electrophoresis analysis revealed DNA cleavages( DNA ladder). The results indicated that tea polyphenole could induce apoptosis of cultured human hepatocellular carcinoma cell line HepG-Ⅱ. It suggested that tea polyphenols could be used to treat human liver carcinoma.

We examined eutopic,ectopic endometria and peritoneal fluid macrophages from 22 patients with endometriosis(EMS) and 14 women without EMS.To obtain evidence for the induction of programmed cell death,apoptotic cells were identified using a modified terminal deixynucleotidyltransferasebiotin nick end-labeling method(TUNEL).To evaluate cell death repressor activity,bcl-2,bax and fas genes expression was examined using immunohistochemical staining. The results showed that bcl-2 expression in eutopic,ectopic endometrium and peritoneal fluid macrophages with EMS was significantly increased compared with no EMS(P＜0.01).Bax expression in eutopic,ectopic and peritoneal fluid macrophages with EMS was significantly decreased compared with no EMS(P＜0.05). Fas expression in eutopic,ectopic endometrium was decreased compared with no EMS(P＜0.05). The expression of apoptotic genes were different in eutopic,ectopic endometrium and peritoneal fluid macrophages from EMS and no EMS.Apoptotic rate in EMS was lower than no EMS,and its acceptance was decreased,which could bear implications for the growth and survival of ectopic endometrial tissue.

To explore the influence of angiotensinⅡ(AngⅡ) and aldosterone (Ald) on synthesis of collagen and activity of precollagenase secreted by cardiac fibroblasts.Sirus Red method was employed to evaluate collagen synthesis of cultured cardiac fibroblasts; typeⅠ collagen was measured by competitive ELISA; collagenase activity was tested by fluorescence spectrophotometry.At the doses of 10-9～10-7mol/L, AngⅡ could enhance gross and typeⅠ collagen production in a dose-dependent manner. Collagenase activity decreased with increasing concentration of AngⅡ. These effects of AngⅡcould be completely abolished by its receptor antagonist, saralasin. Ald might enhance gross and typeⅠ collagen production only at a higher concentration (10-7～10-8mol/L), whereas lower concentration (10-9mol/L) had no effect on it. These effects of Ald could be abolished by spironolactone,a specific Ald receptor antagonist. In addition, Ald could significantly decrease the activity of collagenase secreted from cultured cardiac fiboblasts at concentrations of 10-9～10-7mol/L within 24h，which could not be antagonized by 10-6mol/L spironolactone. The results suggested that AngⅡand Ald enhance cardiac fibroblasts collagen synthesis and inhibit collagenase activity ,resulting in net collagen accumulation resulted and acceleration of cardiac fibrosis.

This paper investigate the methods to detect engraftment rate of the four patients with hematological disorders who accepted nonmyeloablative allogeneic peripheral blood stem cell transplantation(NAPBSCT). To find out the best method, their engraftment rates were detected serially at different time after NAPBSCT by means of either FISH, or conventional chromosome analysis combined with R-banding analysis concurrently.The results were carefully compared with one another. All these four sex-mismatched cases were engrafted partially,and two of them changed to full engrafment. The results show no statistically significant difference in 3 groups (conventional method, FISH for hypermetaphase, FISH for interphase nuclei). But the results strongly indicate that FISH is a rapid, precise, objective,and reliable menthod for detection of the engraftment rate,and it is suitable for sex-mismatched NAPBSCT.

The author briefs the main research progress and way of the new developed nonmyeloablative allogeneic stem cell transplantation(NAST).The definition,conditioning regimen ,graft versus host disease(GVHD),graft versus leukemia(GVL) effects and clinical research are disussed.NAST is developed from theories and ideas of tranditional allogeneic stem cell transplantation,is a new way for hematological diseases ,malignant tumors and congenital immunodeficiencies.The author proposes cooperative researches on problems related with NAST.

42cases with spondylotisthesis were treated with RF system. The types of spondylotisthesis were investigated and result was analysed. The results : follow-up of an average of 5 years showed obvious relief of the symptoms. It suggested that RF system has the advantages of high reduction rate restoration of the intervertebral height and lumbo-sacral physiological curvature and easy manipulation.

Periosteal autograft from different sources have been used to repair 1.5cm bone defects of radius in 10 rabbits.On the left side,sharp-dissected grafts were implanted and the animals were sacrificed 4,8,14,30 and 60 days after operation.Bone formation was studied with X-ray and histologic technique.The results showed that the way of sharp-dissecting can preserve the periosteum completely,and good bone formation was found in this group.It suggested that the sharp-dissecting of the periosteum is the important key for periosteal graft.

To investigate the temporal variation of cell-mediated immunity in injured tissue of maxillofacial firearm trauma. Cells expressing messenger RNA(mRNA) for IL-2 were demonstrated by dot blotting and in situ hybrization in injured tissue of maxillofacial trauma inflicted by bullet, in conjunction with immunohistochemistry for IL-2 in normal and postinjury conditions. It was found that, apart from a transient eleratim in level within 24h post injury, both IL-2 production and IL-2 mRNA expression were consistently suppressed in cells at the site of the wound compared with the normal. The changes in the tissues of indirect injury by the projectile were significant. These results indicate that the cell-mediated immunity was suppressed in maxillofacial firearm trauma and the principal cellular abnormalities that resulted in altered T cell activation and IL-2 production postinjury was downregulation of mRNA before IL-2 gene transcription. It also suggested that the indirect injury by projectile contribute mainly to the depression of cell-mediated immunity.