This study aimed to find out that a cup of tea is or is not safe for human health from microbial contamination and to point out the antimicrobial property of made tea liquor. Different made tea brands were collected randomly from different super shop of Dhaka city. The Association of Official Agricultural Chemists (AOAC), 2005 was used as official methods of analysis. The Standard Plate Count (SPC) technique was used for total microbial load, yeast and fungal count. Most Probable Number (MPN) technique was used for the enumeration of coliform in tea samples. Bacteria, yeast, mould and coliform were observed before and after boiling in all studied the samples. Before boiling, total microbial load and coliform were found at significantly higher of its’ acceptable limit (p<0.05) whereas yeast and fungus were found of its’ acceptable limit (p>0.05). After boiling, only coliform was observed significantly higher in all except Tetley tea at its’ non-acceptable limit (p<0.05). Fecal coliform was not present at every stage of this study. Made tea liquor has shown to have antimicrobial property. Boiling in tea preparation and its’ liquor antimicrobial property considerably reduced the level of microbial load to safe level for public consumption.

Objective: To investigate the therapeutic effects of methanol extract of Citrus macroptera Montr. fruit in α-amylase inhibitory activity (in vitro) and hypoglycemic activity in normal and glucose induced hyperglycemic rats (in vivo). Methods: Fruits of Citrus macroptera without rind was extracted with pure methanol following cold extraction and tested for presence of phytochemical constituents, α-amylase inhibitory activity, and hypoglycemic effect in normal rats and glucose induced hyperglycemic rats. Results: Presence of saponin, steroid and terpenoid were identified in the extract. The results showed that fruit extract had moderate α-amylase inhibitory activity [IC

OBJECTIVETo investigate the therapeutic effects of methanol extract of Citrus macroptera Montr.fruit in α-amylase inhibitory activity (in vitro) and hypoglycemic activity in normal and glucose induced hyperglycemic rats (in vivo).

METHODSFruits of Citrus macroptera without rind was extracted with pure methanol following cold extraction and tested for presence of phytochemical constituents, α-amylase inhibitory activity, and hypoglycemic effect in normal rats and glucose induced hyperglycemic rats.

RESULTSPresence of saponin, steroid and terpenoid were identified in the extract. The results showed that fruit extract had moderate α-amylase inhibitory activity [IC50 value=(3.638±0.190) mg/mL] as compared to acarbose. Moreover at 500 mg/kg and 1 000 mg/kg doses fruit extract significantly (P<0.05 and P<0.01 respectively) reduced fasting blood glucose level in normal rats as compared to glibenclamide (5 mg/kg). In oral glucose tolerance test, 500 mg/kg dose significantly reduced blood glucose level (P<0.05) at 2 h but 1 000 mg/kg dose significantly reduced blood glucose level at 2 h and 3 h (P<0.05 and P<0.01 respectively) whereas glibenclamide (5 mg/kg) significantly reduced glucose level at every hour after administration. Overall time effect is also considered extremely significant with F value=23.83 and P value=0.0001 in oral glucose tolerance test.

CONCLUSIONThese findings suggest that the plant may be a potential source for the development of new oral hypoglycemic agent.